Prupe.4G086700_v2.0.a1

Tyrosine dopa decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
N/A
Physical Location & Seq
Forward (+)
4267511 .. 4269043
1533 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G086700.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 1533 bp
ATGGGTAGCCTAAATTTCGACCATCCGCAAGAAAACAACTCTGCCCACATGTCTGGCCCTCTGGACCTTGTGGAGTTGAGAAGACAAGGCCACATGATAATAGATTTCATTACTGATTATTACCAAAACATAGAGAAACACCCAGTTCTTAGCCAAGTCCAACCAGGCTATCTCAAACAACGCTTGCCAGAATCTGCCCCATACAACCCTGAACCCATTGAAACCATCCTCCGAGACGTGCAGGATCATATTGTCCCTGGCCTAACACATTGGCAAAGCCCTAACCACTTTGCCTACTTCCCTGCAACTATCAGCACCGCAGGGTTTCTCGGCGAAATGCTCACCACTTGCTTCAATGTTGTGGGGTTCAACTGGATGGCATCCCCAGCTGCAACTGAACTCGAAACCATTGTCATGGATTGGCTTGGAGATATGCTCAAACTTCCCAACTCTTTCCTCTTCTCCGGCACCGGCGGCGGTGTGTTGCATGGAAGTACTCATGAGTCTGTCGTTTGCACCATGGCAGCTGCCAGGGACCAAATCCTGAGCCGAATTGGTGAAGAGAATATAGGGAAACTTGTGGTGTACGGCTCTGATCAAACACATAGCGTGATTCAAAAAGTGTCCCAAATTGTTGGGATCCCATCAAAGAACTTCCGGGCCATCGAGACCACAATATCATCATCCTTTACACTGTCCCCTGAAACCCTACGGTTGACTGTTTGTTCAGACATGGAAGCTGGGTTGGTTCCATTCTATCTATGCGCCACCGTGGGAACAACCGCAACCACAGCCGTCGATCCGCTGGGGCCACTCTGCGACGTGGCAAAAGATTACGGCATGTGGGTACATGTGGACGCTGCATATGCCGGAAGTGCTTGCATTTGCCCCGAGTTTCGGCAGTATATCGATGGCATTGAGGGTGCAAATTCGTTCAGCTTCAATGCGCAGAAATGGTTCTTTACAGCTCTCGACTGTTGTTGCCTTTGGGTTAAGAATCCGAGTGCGTTGACAAAATCAATGTCAACGGATCTTGAGGTGTTGAGGAACAAGGCCAGTGAGTCGAAGCGAGTGGTGGACTTCAAAGACTGGCAAATAGCCCTAACCCGGAGGTTCCGGGCCATCAAGCTGTGGCTTGTGCTCAGGAGCTACGGGGTGGCTAATCTTAGGAACTTCCTCAGAAGCCATGTGAAAATGGCGAAGCGTTTTGAAGGGCTTGTGAGGACGGACGAAAGGTTTGAGGTTGTGGTGCCTAGGATTTTTGCTTTGGTGTGTTTTAGGATTTCACCATCCGCAATTAGTAAAGCTAATCCAACTCCAAGTGATGAGAAATGTGTAAACGAAGTAAATTGCAAGTTGCTGGAGGCGATCAATGGGTCGGGTTGGGTGTACATGACCCATGCTGTGGTTGGAGGGATGTATGTGTTGCGTTGCGCCATTGGAGCAAGTCTCACTAAGGAAAAGCACGTAGCCATGGCTTGGAAGGTGGTGCAAGAACATGTAGATGCCATCCTCCCCCTTACCATGTATTAA

Protein Analysis

511

Amino Acids

56.65

Weight (kDa)

6.32

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 948
AccB1I GGYRCC 2 cut(s) 467, 1249
AccB7I CCANNNNNTGG 2 cut(s) 1405, 1479
AciI CCGC 7 cut(s) 26, 318, 474, 477, 783, 803, 1293
AclWI GGATC 5 cut(s) 252, 634, 647, 794, 1038
AcsI RAATTY 2 cut(s) 13, 928
AfaI GTAC 4 cut(s) 496, 587, 849, 1391
AfiI CCNNNNNNNGG 4 cut(s) 897, 1107, 1405, 1479
AflIII ACRYGT 3 cut(s) 48, 850, 1498
AgsI TTSAA 7 cut(s) 221, 355, 370, 617, 943, 1084, 1211
AjiI CACGTC 2 cut(s) 238, 823
AjnI CCWGG 3 cut(s) 163, 256, 530
AluBI AGCT 8 cut(s) 389, 527, 740, 939, 968, 1129, 1149, 1307
AluI AGCT 8 cut(s) 389, 527, 740, 939, 968, 1129, 1149, 1307
Alw21I GWGCWC 1 cut(s) 1143
Alw26I GTCTC 3 cut(s) 228, 662, 1454
AlwI GGATC 5 cut(s) 252, 634, 647, 794, 1038
AlwNI CAGNNNCTG 1 cut(s) 194
Ama87I CYCGRG 1 cut(s) 890
AoxI GGCC 7 cut(s) 55, 88, 259, 660, 809, 1053, 1119
ApeKI GCWGC 4 cut(s) 389, 524, 527, 860
ApoI RAATTY 2 cut(s) 13, 928
ArsI GACNNNNNNTTYG 6 cut(s) 396, 428, 1007, 1039, 1220, 1252
Asp700I GAANNNNTTC 1 cut(s) 956
AspA2I CCTAGG 1 cut(s) 1253
AspLEI GCGC 3 cut(s) 767, 949, 1436
AspS9I GGNCC 6 cut(s) 56, 64, 535, 660, 809, 1119
AsuC2I CCSGG 3 cut(s) 659, 1108, 1118
AsuHPI GGTGA 3 cut(s) 334, 569, 1278
AvaI CYCGRG 1 cut(s) 890
AvaII GGWCC 2 cut(s) 64, 535
AvrII CCTAGG 1 cut(s) 1253
BamHI GGATCC 1 cut(s) 639
BanI GGYRCC 2 cut(s) 467, 1249
BbsI GAAGAC 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 1143
BbvI GCAGC 4 cut(s) 376, 514, 536, 847
BccI CCATC 9 cut(s) 30, 233, 370, 652, 671, 905, 1130, 1297, 1517
BceAI ACGGC 3 cut(s) 604, 779, 853
BciT130I CCWGG 3 cut(s) 165, 258, 532
BclI TGATCA 1 cut(s) 595
BcnI CCSGG 3 cut(s) 659, 1108, 1118
BcoDI GTCTC 3 cut(s) 228, 662, 1454
BfaI CTAG 1 cut(s) 1254
BisI GCNGC 5 cut(s) 390, 475, 525, 528, 861
BlnI CCTAGG 1 cut(s) 1253
BlsI GCNGC 5 cut(s) 391, 476, 526, 529, 862
BmcAI AGTACT 1 cut(s) 496
Bme1390I CCNGG 6 cut(s) 165, 258, 532, 659, 1108, 1118
Bme18I GGWCC 2 cut(s) 64, 535
BmeT110I CYCGRG 1 cut(s) 890
BmgBI CACGTC 2 cut(s) 238, 823
BmgT120I GGNCC 6 cut(s) 56, 64, 535, 660, 809, 1119
BmiI GGNNCC 7 cut(s) 469, 536, 641, 750, 810, 1115, 1251
BmrFI CCNGG 6 cut(s) 165, 258, 532, 659, 1108, 1118
BmrI ACTGGG 1 cut(s) 137
BmsI GCATC 2 cut(s) 389, 1495
BmuI ACTGGG 1 cut(s) 137
BpiI GAAGAC 1 cut(s) 88
BplI GAGNNNNNCTC 4 cut(s) 420, 452, 1434, 1466
BpmI CTGGAG 1 cut(s) 1382
Bpu10I CCTNAGC 2 cut(s) 545, 1142
BpuEI CTTGAG 1 cut(s) 1055
BpuMI CCSGG 3 cut(s) 659, 1108, 1118
Bsa29I ATCGAT 1 cut(s) 909
BsaAI YACGTR 1 cut(s) 1468
BsaI GGTCTC 1 cut(s) 662
BsaJI CCNNGG 6 cut(s) 256, 519, 531, 771, 1253, 1473
Bsc4I CCNNNNNNNGG 4 cut(s) 897, 1107, 1405, 1479
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 4 cut(s) 143, 377, 1056, 1094
BseBI CCWGG 3 cut(s) 165, 258, 532
BseCI ATCGAT 1 cut(s) 909
BseDI CCNNGG 6 cut(s) 256, 519, 531, 771, 1253, 1473
BseGI GGATG 8 cut(s) 22, 225, 380, 381, 683, 1289, 1422, 1509
BseLI CCNNNNNNNGG 4 cut(s) 897, 1107, 1405, 1479
BseMII CTCAG 3 cut(s) 536, 1156, 1192
BseNI ACTGG 4 cut(s) 143, 377, 1056, 1094
BseXI GCAGC 4 cut(s) 376, 514, 536, 847
BseYI CCCAGC 3 cut(s) 385, 740, 805
BsgI GTGCAG 1 cut(s) 260
BshFI GGCC 7 cut(s) 57, 90, 261, 662, 811, 1055, 1121
BshNI GGYRCC 2 cut(s) 467, 1249
BshVI ATCGAT 1 cut(s) 909
BsiHKAI GWGCWC 1 cut(s) 1143
BsiHKCI CYCGRG 1 cut(s) 890
BsiSI CCGG 6 cut(s) 465, 471, 658, 870, 1108, 1117
BslFI GGGAC 4 cut(s) 239, 548, 610, 682
BslI CCNNNNNNNGG 4 cut(s) 897, 1107, 1405, 1479
BsmAI GTCTC 3 cut(s) 228, 662, 1454
BsmBI CGTCTC 1 cut(s) 228
BsmFI GGGAC 4 cut(s) 239, 548, 610, 682
BsnI GGCC 7 cut(s) 57, 90, 261, 662, 811, 1055, 1121
Bso31I GGTCTC 1 cut(s) 662
BsoBI CYCGRG 1 cut(s) 890
Bsp1286I GDGCHC 1 cut(s) 1143
Bsp1407I TGTACA 1 cut(s) 1389
Bsp143I GATC 6 cut(s) 244, 595, 639, 799, 1030, 1368
Bsp19I CCATGG 2 cut(s) 519, 1473
BspACI CCGC 7 cut(s) 26, 318, 474, 477, 783, 803, 1293
BspANI GGCC 7 cut(s) 57, 90, 261, 662, 811, 1055, 1121
BspCNI CTCAG 3 cut(s) 537, 1155, 1191
BspDI ATCGAT 1 cut(s) 909
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 7 cut(s) 469, 536, 641, 750, 810, 1115, 1251
BspPI GGATC 5 cut(s) 252, 634, 647, 794, 1038
BspT107I GGYRCC 2 cut(s) 467, 1249
BspTNI GGTCTC 1 cut(s) 662
BsrFI RCCGGY 1 cut(s) 470
BsrGI TGTACA 1 cut(s) 1389
BsrI ACTGG 4 cut(s) 143, 377, 1056, 1094
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 6 cut(s) 256, 519, 531, 771, 1253, 1473
BssMI GATC 6 cut(s) 244, 595, 639, 799, 1030, 1368
BssT1I CCWWGG 3 cut(s) 519, 1253, 1473
Bst2UI CCWGG 3 cut(s) 165, 258, 532
Bst4CI ACNGT 5 cut(s) 696, 714, 721, 772, 977
Bst6I CTCTTC 2 cut(s) 464, 555
BstAUI TGTACA 1 cut(s) 1389
BstBAI YACGTR 1 cut(s) 1468
BstC8I GCNNGC 2 cut(s) 185, 880
BstDEI CTNAG 6 cut(s) 149, 545, 1142, 1166, 1178, 1455
BstDSI CCRYGG 3 cut(s) 519, 771, 1473
BstF5I GGATG 8 cut(s) 22, 225, 380, 381, 683, 1289, 1422, 1509
BstHHI GCGC 3 cut(s) 767, 949, 1436
BstKTI GATC 6 cut(s) 247, 598, 642, 802, 1033, 1371
BstMAI GTCTC 3 cut(s) 228, 662, 1454
BstMBI GATC 6 cut(s) 244, 595, 639, 799, 1030, 1368
BstMWI GCNNNNNNNGC 6 cut(s) 386, 474, 791, 866, 875, 1442
BstNI CCWGG 3 cut(s) 165, 258, 532
BstNSI RCATGY 4 cut(s) 52, 844, 854, 1502
BstSCI CCNGG 6 cut(s) 163, 256, 530, 657, 1106, 1116
BstV1I GCAGC 4 cut(s) 376, 514, 536, 847
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 2 cut(s) 639, 1030
BstXI CCANNNNNNTGG 3 cut(s) 53, 415, 635
BstYI RGATCY 2 cut(s) 639, 1030
Bsu15I ATCGAT 1 cut(s) 909
BsuRI GGCC 7 cut(s) 57, 90, 261, 662, 811, 1055, 1121
BsuTUI ATCGAT 1 cut(s) 909
BtgI CCRYGG 3 cut(s) 519, 771, 1473
BtrI CACGTC 2 cut(s) 238, 823
BtsCI GGATG 8 cut(s) 22, 225, 380, 381, 683, 1289, 1422, 1509
BtsIMutI CAGTG 2 cut(s) 692, 1063
Cac8I GCNNGC 2 cut(s) 185, 880
CaiI CAGNNNCTG 1 cut(s) 194
CciI TCATGA 1 cut(s) 499
CfoI GCGC 3 cut(s) 767, 949, 1436
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 6 cut(s) 56, 64, 535, 660, 809, 1119
ClaI ATCGAT 1 cut(s) 909
CseI GACGC 1 cut(s) 866
Csp6I GTAC 4 cut(s) 495, 586, 848, 1390
CviQI GTAC 4 cut(s) 495, 586, 848, 1390
DdeI CTNAG 6 cut(s) 149, 545, 1142, 1166, 1178, 1455
DpnI GATC 6 cut(s) 246, 597, 641, 801, 1032, 1370
DpnII GATC 6 cut(s) 244, 595, 639, 799, 1030, 1368
Eam1104I CTCTTC 2 cut(s) 464, 555
EarI CTCTTC 2 cut(s) 464, 555
Eco130I CCWWGG 3 cut(s) 519, 1253, 1473
Eco31I GGTCTC 1 cut(s) 662
Eco47I GGWCC 2 cut(s) 64, 535
Eco88I CYCGRG 1 cut(s) 890
EcoRII CCWGG 3 cut(s) 163, 256, 530
EcoT14I CCWWGG 3 cut(s) 519, 1253, 1473
ErhI CCWWGG 3 cut(s) 519, 1253, 1473
Esp3I CGTCTC 1 cut(s) 228
FaqI GGGAC 4 cut(s) 239, 548, 610, 682
FauNDI CATATG 1 cut(s) 865
FbaI TGATCA 1 cut(s) 595
Fnu4HI GCNGC 5 cut(s) 390, 475, 525, 528, 861
FokI GGATG 8 cut(s) 9, 212, 367, 388, 670, 1276, 1429, 1496
Fsp4HI GCNGC 5 cut(s) 390, 475, 525, 528, 861
FspBI CTAG 1 cut(s) 1254
FspI TGCGCA 1 cut(s) 948
GlaI GCGC 3 cut(s) 766, 948, 1435
GluI GCNGC 5 cut(s) 390, 475, 525, 528, 861
GsaI CCCAGC 3 cut(s) 389, 744, 809
GsuI CTGGAG 1 cut(s) 1382
HaeIII GGCC 7 cut(s) 57, 90, 261, 662, 811, 1055, 1121
HapII CCGG 6 cut(s) 465, 471, 658, 870, 1108, 1117
HgaI GACGC 1 cut(s) 866
HhaI GCGC 3 cut(s) 767, 949, 1436
Hin6I GCGC 3 cut(s) 765, 947, 1434
HinP1I GCGC 3 cut(s) 765, 947, 1434
HincII GTYRAC 3 cut(s) 717, 1011, 1026
HindII GTYRAC 3 cut(s) 717, 1011, 1026
HinfI GANTC 5 cut(s) 191, 503, 613, 997, 1061
HpaII CCGG 6 cut(s) 465, 471, 658, 870, 1108, 1117
HphI GGTGA 3 cut(s) 334, 569, 1278
Hpy166II GTNNAC 8 cut(s) 586, 717, 856, 1011, 1026, 1078, 1339, 1390
Hpy188I TCNGA 5 cut(s) 233, 595, 730, 1002, 1181
Hpy188III TCNNGA 7 cut(s) 62, 500, 544, 667, 971, 1034, 1144
Hpy8I GTNNAC 8 cut(s) 586, 717, 856, 1011, 1026, 1078, 1339, 1390
Hpy99I CGWCG 2 cut(s) 800, 824
HpyAV CCTTC 2 cut(s) 1205, 1477
HpyCH4III ACNGT 5 cut(s) 696, 714, 721, 772, 977
HpyCH4IV ACGT 3 cut(s) 237, 822, 1467
HpyF10VI GCNNNNNNNGC 6 cut(s) 386, 474, 791, 866, 875, 1442
HpyF3I CTNAG 6 cut(s) 149, 545, 1142, 1166, 1178, 1455
HpySE526I ACGT 3 cut(s) 237, 822, 1467
HspAI GCGC 3 cut(s) 765, 947, 1434
Ksp22I TGATCA 1 cut(s) 595
Kzo9I GATC 6 cut(s) 244, 595, 639, 799, 1030, 1368
LmnI GCTCC 2 cut(s) 1146, 1442
Lsp1109I GCAGC 4 cut(s) 376, 514, 536, 847
LweI GCATC 2 cut(s) 389, 1495
MaeI CTAG 1 cut(s) 1254
MaeII ACGT 3 cut(s) 237, 822, 1467
MalI GATC 6 cut(s) 246, 597, 641, 801, 1032, 1370
MboI GATC 6 cut(s) 244, 595, 639, 799, 1030, 1368
MboII GAAGA 3 cut(s) 93, 451, 572
MflI RGATCY 2 cut(s) 639, 1030
MhlI GDGCHC 1 cut(s) 1143
MluCI AATT 6 cut(s) 13, 552, 630, 928, 1296, 1348
MlyI GAGTC 2 cut(s) 512, 1070
MmeI TCCRAC 3 cut(s) 184, 1337, 1390
MroXI GAANNNNTTC 1 cut(s) 956
MseI TTAA 2 cut(s) 993, 1531
MslI CAYNNNNRTG 2 cut(s) 413, 1503
MspA1I CMGCKG 3 cut(s) 389, 527, 805
MspI CCGG 6 cut(s) 465, 471, 658, 870, 1108, 1117
MspR9I CCNGG 6 cut(s) 165, 258, 532, 659, 1108, 1118
MvaI CCWGG 3 cut(s) 165, 258, 532
MwoI GCNNNNNNNGC 6 cut(s) 386, 474, 791, 866, 875, 1442
NciI CCSGG 3 cut(s) 659, 1108, 1118
NcoI CCATGG 2 cut(s) 519, 1473
NdeI CATATG 1 cut(s) 865
NdeII GATC 6 cut(s) 244, 595, 639, 799, 1030, 1368
NlaIV GGNNCC 7 cut(s) 469, 536, 641, 750, 810, 1115, 1251
NmeAIII GCCGAG 1 cut(s) 309
NsbI TGCGCA 1 cut(s) 948
NspI RCATGY 4 cut(s) 52, 844, 854, 1502
PagI TCATGA 1 cut(s) 499
PciI ACATGT 3 cut(s) 48, 850, 1498
PdmI GAANNNNTTC 1 cut(s) 956
PfeI GAWTC 3 cut(s) 191, 613, 997
PflMI CCANNNNNTGG 2 cut(s) 1405, 1479
PkrI GCNGC 5 cut(s) 391, 476, 526, 529, 862
PleI GAGTC 2 cut(s) 511, 1069
PpsI GAGTC 2 cut(s) 511, 1069
Ppu21I YACGTR 1 cut(s) 1468
PscI ACATGT 3 cut(s) 48, 850, 1498
Psp6I CCWGG 3 cut(s) 163, 256, 530
PspFI CCCAGC 3 cut(s) 385, 740, 805
PspGI CCWGG 3 cut(s) 163, 256, 530
PspN4I GGNNCC 7 cut(s) 469, 536, 641, 750, 810, 1115, 1251
PspPI GGNCC 6 cut(s) 56, 64, 535, 660, 809, 1119
PstNI CAGNNNCTG 1 cut(s) 194
PsuI RGATCY 2 cut(s) 639, 1030
PvuII CAGCTG 2 cut(s) 389, 527
RsaI GTAC 4 cut(s) 496, 587, 849, 1391
RsaNI GTAC 4 cut(s) 495, 586, 848, 1390
RseI CAYNNNNRTG 2 cut(s) 413, 1503
SaqAI TTAA 2 cut(s) 993, 1531
SatI GCNGC 5 cut(s) 390, 475, 525, 528, 861
Sau3AI GATC 6 cut(s) 244, 595, 639, 799, 1030, 1368
Sau96I GGNCC 6 cut(s) 56, 64, 535, 660, 809, 1119
ScaI AGTACT 1 cut(s) 496
SchI GAGTC 2 cut(s) 512, 1070
ScrFI CCNGG 6 cut(s) 165, 258, 532, 659, 1108, 1118
SduI GDGCHC 1 cut(s) 1143
SfaNI GCATC 2 cut(s) 389, 1495
SgrAI CRCCGGYG 1 cut(s) 470
SinI GGWCC 2 cut(s) 64, 535
SmiMI CAYNNNNRTG 2 cut(s) 413, 1503
SmlI CTYRAG 1 cut(s) 1034
SmoI CTYRAG 1 cut(s) 1034
Sse9I AATT 6 cut(s) 13, 552, 630, 928, 1296, 1348
SsiI CCGC 7 cut(s) 26, 318, 474, 477, 783, 803, 1293
SspMI CTAG 1 cut(s) 1254
StyD4I CCNGG 6 cut(s) 163, 256, 530, 657, 1106, 1116
StyI CCWWGG 3 cut(s) 519, 1253, 1473
TaaI ACNGT 5 cut(s) 696, 714, 721, 772, 977
TaiI ACGT 3 cut(s) 240, 825, 1470
TaqI TCGA 7 cut(s) 18, 402, 666, 798, 909, 972, 1064
TasI AATT 6 cut(s) 13, 552, 630, 928, 1296, 1348
TatI WGTACW 2 cut(s) 494, 1389
TauI GCSGC 1 cut(s) 477
TfiI GAWTC 3 cut(s) 191, 613, 997
Tru1I TTAA 2 cut(s) 993, 1531
Tru9I TTAA 2 cut(s) 993, 1531
TscAI CASTG 2 cut(s) 699, 1063
TseI GCWGC 4 cut(s) 389, 524, 527, 860
TspDTI ATGAA 1 cut(s) 97
TspGWI ACGGA 2 cut(s) 1043, 1241
TspRI CASTG 2 cut(s) 699, 1063
Van91I CCANNNNNTGG 2 cut(s) 1405, 1479
VpaK11BI GGWCC 2 cut(s) 64, 535
XapI RAATTY 2 cut(s) 13, 928
XceI RCATGY 4 cut(s) 52, 844, 854, 1502
XcmI CCANNNNNNNNNTGG 1 cut(s) 1406
XmaJI CCTAGG 1 cut(s) 1253
XmnI GAANNNNTTC 1 cut(s) 956
XspI CTAG 1 cut(s) 1254
ZrmI AGTACT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.