Prupe.4G094000_v2.0.a1

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
N/A
Physical Location & Seq
Reverse (-)
4736720 .. 4739998
3279 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G094000.1_v2.0.a1.CDS.3

Sequence Viewer

Length: 1722 bp
ATGCGACTGATCTCATTGCTAGTAATAGTTCTAGAGCTAGTGTTTGTATTAGCTATTAGTACATCATCAACAACAGCAGTAGGTGATCATCAAGCCCTATTGCCTCAAGCCTTGCCTGGCTGCTCTGACCACTGTGGCAACATCACAATCCCATATCCATTCGGCATGGCTAGAGGTTGTTACGGCCGACCCGAGTTTTTTGTCAATTGTACTGCTACTCCTACTACCACTACCGATATTAATTTTAAAGATCTACCTCCTATGCCTGTATATAAACTTACAAGTTACATCCCTAGTGGATTGTTCCCGTTTGAGATGGTATTGGAGCTATGGTACTCTAATCATTTATACTCATGCAGCTATGGCTTCTTTGTGGAACAAGGCGAGTTCACATTCTCCCCCAACATAAGTTTGCAACAACTCAACAAGACTAGCCACCTTCCTTTGGTTCTCAATTGGGAAATTGGGGATCATGGCCCATGCAGTTCAGCTAATAACAGCACGGATTTTGCATGCAAGGGAAATAGCACGTGCGTCAGTTGGCCCAATATCAATGGCTGGTCTACTGGTTACATTTGCTGGTGCTTGCCAGGTTACCAAGGAAACCCATACCACCCAGATGGTTGCCAAGCTATAGGTGTCATGTTGGTTTTACTCGTCGGATCTGCATGGTCCCTATGCTGGGGAATGAAGCAACGAAAGTACATCAAGCTCAAAGAAAAGTACTTCGAAGAAAATGGTGGTTTCTTGTTACAACATAAGCTTGCTAGTCATCATGTGGTTGAGACAACCAGGATCTGTACTGCAGAAGAGCTGGAAAAAGCCACAAACAATTATCACGACAGTAGAGTCCTTGGGAAAGGATCTTATGGAATAGTGTACAAAGGAATATTACCAGATAAGAAAATGGTTGCCATAAAAAAGTCCAGAATTGCTGCCCCAACCCAGACCGAGCAGTTTGTTAATGAGTTGGTTGTTCTTTCTCAAATCAACCATAGAAATGTGGTGAGGCTATTAGGTTGTTGTTTAGAGTCAGAAGTGCCTTTACTAGTTTATGAGTTCATCACCAACGGCACTCTTTTTGAGCACATTCATGGTGAGAGGAGCAGAGGATCATCGCTTGTGTGGGAATTGCGATTGAAGATTGCAGCAGAAACTGCAGGAGCACTAGCATACTTACACTCATCCACTTTCATGCAAATCATAAACCGAGATATCAGCCAAAATTGCCTCGTACATCAAACTCAAGTAGTAACTTTGGTGCAAGGGACACTTGGATACTTAGACCCTGAGTATCTCCACTCAAACCAACTAACAGAGAAGAGTGATGTTTATAGCTTCGGAGTTGTCCTTGTAGAGCTATTGACAAGCAAAGTGGCAATTTCTTATGATAGGCCTGAGGCAGAGAGAAAAGATCGCTTGGAACGTATTCTTGATTGTCGCATAGTCAACAAACCGAATATTGAGACAGTAAGAAATGTGGCCCGTCTTGCAAAAAGATGTTTAAGGTTAAAAGGGGAGGAAAGGCCGACCATGAAAGACGTAGCTATGGAGCTAGAGGGAATGAGAATAGTGGCAAACCATCCATGGGGAAGTAATGTTGATTTGCGTTCAGAAGATCAGACTTTGCTCTTGTCGTGGCCACCTTCAGACGCTTCTGTTGCTGACGTTAGAGATGATTGTGGTTCTACTGCCACAACTTCTGGTACAACTAGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

574

Amino Acids

63.89

Weight (kDa)

6.29

Isoelectric Point (pI)

34.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 848
AccI GTMKAC 1 cut(s) 563
AclWI GGATC 5 cut(s) 477, 670, 803, 871, 1120
AcoI YGGCCR 2 cut(s) 184, 1640
AcuI CTGAAG 1 cut(s) 1632
AcvI CACGTG 1 cut(s) 531
AfaI GTAC 9 cut(s) 61, 211, 335, 704, 725, 802, 881, 1236, 1708
AfiI CCNNNNNNNGG 4 cut(s) 445, 681, 682, 1588
AgsI TTSAA 1 cut(s) 1141
AhlI ACTAGT 1 cut(s) 1048
AjnI CCWGG 3 cut(s) 115, 589, 791
Alw21I GWGCWC 2 cut(s) 1089, 1168
Alw26I GTCTC 2 cut(s) 779, 1460
AlwI GGATC 5 cut(s) 477, 670, 803, 871, 1120
AlwNI CAGNNNCTG 2 cut(s) 798, 1157
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 7 cut(s) 184, 475, 542, 1394, 1482, 1526, 1640
ApeKI GCWGC 4 cut(s) 120, 357, 935, 1148
AseI ATTAAT 1 cut(s) 240
Asp700I GAANNNNTTC 1 cut(s) 1428
AspS9I GGNCC 4 cut(s) 476, 543, 672, 1483
AsuHPI GGTGA 4 cut(s) 95, 1018, 1057, 1109
AsuII TTCGAA 1 cut(s) 729
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 1 cut(s) 672
AxyI CCTNAGG 1 cut(s) 1398
BalI TGGCCA 1 cut(s) 1642
BarI GAAGNNNNNNTAC 2 cut(s) 1029, 1061
BbrPI CACGTG 1 cut(s) 531
Bbv12I GWGCWC 2 cut(s) 1089, 1168
BbvI GCAGC 4 cut(s) 107, 369, 922, 1160
BccI CCATC 3 cut(s) 310, 614, 1590
BceAI ACGGC 2 cut(s) 199, 1087
BciT130I CCWGG 3 cut(s) 117, 591, 793
BciVI GTATCC 1 cut(s) 1271
BclI TGATCA 1 cut(s) 85
BcoDI GTCTC 2 cut(s) 779, 1460
BcuI ACTAGT 1 cut(s) 1048
BfmI CTRYAG 3 cut(s) 633, 804, 1158
BfuI GTATCC 1 cut(s) 1271
BglII AGATCT 1 cut(s) 250
BisI GCNGC 4 cut(s) 121, 358, 936, 1149
BlsI GCNGC 4 cut(s) 122, 359, 937, 1150
BmcAI AGTACT 1 cut(s) 725
Bme1390I CCNGG 3 cut(s) 117, 591, 793
Bme18I GGWCC 1 cut(s) 672
BmeT110I CYCGRG 1 cut(s) 191
BmgT120I GGNCC 4 cut(s) 476, 543, 672, 1483
BmiI GGNNCC 1 cut(s) 674
BmrFI CCNGG 3 cut(s) 117, 591, 793
Bpu14I TTCGAA 1 cut(s) 729
BpuEI CTTGAG 2 cut(s) 90, 1230
BsaAI YACGTR 1 cut(s) 531
BsaJI CCNNGG 3 cut(s) 598, 853, 1586
BsaXI ACNNNNNCTCC 4 cut(s) 202, 232, 317, 347
Bsc4I CCNNNNNNNGG 4 cut(s) 445, 681, 682, 1588
Bse1I ACTGG 1 cut(s) 571
Bse21I CCTNAGG 1 cut(s) 1398
Bse3DI GCAATG 1 cut(s) 14
BseBI CCWGG 3 cut(s) 117, 591, 793
BseDI CCNNGG 3 cut(s) 598, 853, 1586
BseGI GGATG 3 cut(s) 288, 1184, 1582
BseLI CCNNNNNNNGG 4 cut(s) 445, 681, 682, 1588
BseMI GCAATG 1 cut(s) 14
BseMII CTCAG 2 cut(s) 1281, 1389
BseNI ACTGG 1 cut(s) 571
BseRI GAGGAG 1 cut(s) 1117
BseX3I CGGCCG 1 cut(s) 184
BseXI GCAGC 4 cut(s) 107, 369, 922, 1160
BseYI CCCAGC 1 cut(s) 681
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 7 cut(s) 186, 477, 544, 1396, 1484, 1528, 1642
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 2 cut(s) 1089, 1168
BsiHKCI CYCGRG 1 cut(s) 191
BslFI GGGAC 2 cut(s) 658, 1282
BslI CCNNNNNNNGG 4 cut(s) 445, 681, 682, 1588
BsmAI GTCTC 2 cut(s) 779, 1460
BsmFI GGGAC 2 cut(s) 658, 1282
BsnI GGCC 7 cut(s) 186, 477, 544, 1396, 1484, 1528, 1642
BsoBI CYCGRG 1 cut(s) 191
Bsp119I TTCGAA 1 cut(s) 729
Bsp1286I GDGCHC 2 cut(s) 1089, 1168
Bsp1407I TGTACA 1 cut(s) 879
Bsp19I CCATGG 1 cut(s) 1586
BspANI GGCC 7 cut(s) 186, 477, 544, 1396, 1484, 1528, 1642
BspCNI CTCAG 2 cut(s) 1282, 1390
BspLI GGNNCC 1 cut(s) 674
BspMAI CTGCAG 2 cut(s) 808, 1162
BspPI GGATC 5 cut(s) 477, 670, 803, 871, 1120
BspQI GCTCTTC 1 cut(s) 804
BspT104I TTCGAA 1 cut(s) 729
BsrDI GCAATG 1 cut(s) 14
BsrGI TGTACA 1 cut(s) 879
BsrI ACTGG 1 cut(s) 571
BssECI CCNNGG 3 cut(s) 598, 853, 1586
BssT1I CCWWGG 3 cut(s) 598, 853, 1586
Bst2UI CCWGG 3 cut(s) 117, 591, 793
Bst4CI ACNGT 3 cut(s) 134, 845, 1471
Bst6I CTCTTC 2 cut(s) 804, 1316
BstAPI GCANNNNNTGC 1 cut(s) 1157
BstAUI TGTACA 1 cut(s) 879
BstBAI YACGTR 1 cut(s) 531
BstBI TTCGAA 1 cut(s) 729
BstC8I GCNNGC 3 cut(s) 514, 587, 765
BstDEI CTNAG 3 cut(s) 1282, 1290, 1398
BstDSI CCRYGG 1 cut(s) 1586
BstEII GGTNACC 1 cut(s) 593
BstF5I GGATG 3 cut(s) 288, 1184, 1582
BstMAI GTCTC 2 cut(s) 779, 1460
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 5 cut(s) 363, 1157, 1227, 1490, 1661
BstNI CCWGG 3 cut(s) 117, 591, 793
BstNSI RCATGY 1 cut(s) 516
BstPI GGTNACC 1 cut(s) 593
BstSCI CCNGG 3 cut(s) 115, 589, 791
BstSFI CTRYAG 3 cut(s) 633, 804, 1158
BstV1I GCAGC 4 cut(s) 107, 369, 922, 1160
BstX2I RGATCY 4 cut(s) 250, 662, 795, 863
BstXI CCANNNNNNTGG 1 cut(s) 620
BstYI RGATCY 4 cut(s) 250, 662, 795, 863
BstZI CGGCCG 1 cut(s) 184
Bsu36I CCTNAGG 1 cut(s) 1398
BsuI GTATCC 1 cut(s) 1271
BsuRI GGCC 7 cut(s) 186, 477, 544, 1396, 1484, 1528, 1642
BtgI CCRYGG 1 cut(s) 1586
BtgZI GCGATG 1 cut(s) 1101
BtsCI GGATG 3 cut(s) 288, 1184, 1582
BtsIMutI CAGTG 1 cut(s) 130
Cac8I GCNNGC 3 cut(s) 514, 587, 765
CaiI CAGNNNCTG 2 cut(s) 798, 1157
Cfr13I GGNCC 4 cut(s) 476, 543, 672, 1483
CseI GACGC 2 cut(s) 523, 1661
Csp6I GTAC 9 cut(s) 60, 210, 334, 703, 724, 801, 880, 1235, 1707
CspCI CAANNNNNGTGG 2 cut(s) 1356, 1391
CviQI GTAC 9 cut(s) 60, 210, 334, 703, 724, 801, 880, 1235, 1707
DdeI CTNAG 3 cut(s) 1282, 1290, 1398
DraI TTTAAA 1 cut(s) 247
DrdI GACNNNNNNGTC 1 cut(s) 848
DseDI GACNNNNNNGTC 1 cut(s) 848
EaeI YGGCCR 2 cut(s) 184, 1640
EagI CGGCCG 1 cut(s) 184
Eam1104I CTCTTC 2 cut(s) 804, 1316
EarI CTCTTC 2 cut(s) 804, 1316
EclXI CGGCCG 1 cut(s) 184
Eco130I CCWWGG 3 cut(s) 598, 853, 1586
Eco147I AGGCCT 1 cut(s) 1396
Eco32I GATATC 1 cut(s) 1216
Eco47I GGWCC 1 cut(s) 672
Eco52I CGGCCG 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 1632
Eco72I CACGTG 1 cut(s) 531
Eco81I CCTNAGG 1 cut(s) 1398
Eco88I CYCGRG 1 cut(s) 191
Eco91I GGTNACC 1 cut(s) 593
EcoO65I GGTNACC 1 cut(s) 593
EcoRII CCWGG 3 cut(s) 115, 589, 791
EcoRV GATATC 1 cut(s) 1216
EcoT14I CCWWGG 3 cut(s) 598, 853, 1586
ErhI CCWWGG 3 cut(s) 598, 853, 1586
FalI AAGNNNNNCTT 2 cut(s) 1257, 1289
FaqI GGGAC 2 cut(s) 658, 1282
FbaI TGATCA 1 cut(s) 85
FblI GTMKAC 1 cut(s) 563
Fnu4HI GCNGC 4 cut(s) 121, 358, 936, 1149
FokI GGATG 3 cut(s) 275, 1171, 1569
Fsp4HI GCNGC 4 cut(s) 121, 358, 936, 1149
GluI GCNGC 4 cut(s) 121, 358, 936, 1149
GsaI CCCAGC 1 cut(s) 685
HaeIII GGCC 7 cut(s) 186, 477, 544, 1396, 1484, 1528, 1642
HgaI GACGC 2 cut(s) 523, 1661
HincII GTYRAC 1 cut(s) 1450
HindII GTYRAC 1 cut(s) 1450
HindIII AAGCTT 1 cut(s) 761
HinfI GANTC 2 cut(s) 849, 1031
HphI GGTGA 4 cut(s) 95, 1018, 1057, 1109
Hpy166II GTNNAC 4 cut(s) 390, 564, 880, 1450
Hpy188I TCNGA 7 cut(s) 127, 662, 1036, 1343, 1615, 1623, 1651
Hpy188III TCNNGA 4 cut(s) 32, 839, 927, 1433
Hpy8I GTNNAC 4 cut(s) 390, 564, 880, 1450
Hpy99I CGWCG 1 cut(s) 662
HpyAV CCTTC 2 cut(s) 449, 1656
HpyCH4III ACNGT 3 cut(s) 134, 845, 1471
HpyCH4IV ACGT 4 cut(s) 530, 1426, 1542, 1668
HpyF10VI GCNNNNNNNGC 5 cut(s) 363, 1157, 1227, 1490, 1661
HpyF3I CTNAG 3 cut(s) 1282, 1290, 1398
HpySE526I ACGT 4 cut(s) 530, 1426, 1542, 1668
Ksp22I TGATCA 1 cut(s) 85
LguI GCTCTTC 1 cut(s) 804
LmnI GCTCC 4 cut(s) 325, 1104, 1163, 1552
Lsp1109I GCAGC 4 cut(s) 107, 369, 922, 1160
MaeII ACGT 4 cut(s) 530, 1426, 1542, 1668
MaeIII GTNAC 6 cut(s) 179, 284, 569, 593, 750, 1252
MboII GAAGA 5 cut(s) 743, 821, 1153, 1333, 1628
MfeI CAATTG 2 cut(s) 205, 454
MflI RGATCY 4 cut(s) 250, 662, 795, 863
MhlI GDGCHC 2 cut(s) 1089, 1168
MlsI TGGCCA 1 cut(s) 1642
MluCI AATT 9 cut(s) 205, 241, 454, 462, 832, 930, 1130, 1225, 1380
MluNI TGGCCA 1 cut(s) 1642
MlyI GAGTC 2 cut(s) 858, 1040
MmeI TCCRAC 1 cut(s) 640
Mox20I TGGCCA 1 cut(s) 1642
MroXI GAANNNNTTC 1 cut(s) 1428
MscI TGGCCA 1 cut(s) 1642
MseI TTAA 5 cut(s) 240, 246, 963, 1505, 1511
MslI CAYNNNNRTG 4 cut(s) 618, 999, 1092, 1193
Msp20I TGGCCA 1 cut(s) 1642
MspR9I CCNGG 3 cut(s) 117, 591, 793
MunI CAATTG 2 cut(s) 205, 454
MvaI CCWGG 3 cut(s) 117, 591, 793
MwoI GCNNNNNNNGC 5 cut(s) 363, 1157, 1227, 1490, 1661
NcoI CCATGG 1 cut(s) 1586
NlaIV GGNNCC 1 cut(s) 674
NspI RCATGY 1 cut(s) 516
NspV TTCGAA 1 cut(s) 729
PaeI GCATGC 1 cut(s) 516
PceI AGGCCT 1 cut(s) 1396
PciSI GCTCTTC 1 cut(s) 804
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 1423
PdmI GAANNNNTTC 1 cut(s) 1428
PkrI GCNGC 4 cut(s) 122, 359, 937, 1150
PleI GAGTC 2 cut(s) 857, 1039
PmaCI CACGTG 1 cut(s) 531
PmlI CACGTG 1 cut(s) 531
PpsI GAGTC 2 cut(s) 857, 1039
Ppu21I YACGTR 1 cut(s) 531
PshBI ATTAAT 1 cut(s) 240
Psp6I CCWGG 3 cut(s) 115, 589, 791
PspCI CACGTG 1 cut(s) 531
PspEI GGTNACC 1 cut(s) 593
PspFI CCCAGC 1 cut(s) 681
PspGI CCWGG 3 cut(s) 115, 589, 791
PspN4I GGNNCC 1 cut(s) 674
PspPI GGNCC 4 cut(s) 476, 543, 672, 1483
PstI CTGCAG 2 cut(s) 808, 1162
PstNI CAGNNNCTG 2 cut(s) 798, 1157
PsuI RGATCY 4 cut(s) 250, 662, 795, 863
RsaI GTAC 9 cut(s) 61, 211, 335, 704, 725, 802, 881, 1236, 1708
RsaNI GTAC 9 cut(s) 60, 210, 334, 703, 724, 801, 880, 1235, 1707
RseI CAYNNNNRTG 4 cut(s) 618, 999, 1092, 1193
SapI GCTCTTC 1 cut(s) 804
SaqAI TTAA 5 cut(s) 240, 246, 963, 1505, 1511
SatI GCNGC 4 cut(s) 121, 358, 936, 1149
Sau96I GGNCC 4 cut(s) 476, 543, 672, 1483
ScaI AGTACT 1 cut(s) 725
SchI GAGTC 2 cut(s) 858, 1040
ScrFI CCNGG 3 cut(s) 117, 591, 793
SduI GDGCHC 2 cut(s) 1089, 1168
SfcI CTRYAG 3 cut(s) 633, 804, 1158
SfuI TTCGAA 1 cut(s) 729
SinI GGWCC 1 cut(s) 672
SmiMI CAYNNNNRTG 4 cut(s) 618, 999, 1092, 1193
SmlI CTYRAG 2 cut(s) 105, 1245
SmoI CTYRAG 2 cut(s) 105, 1245
SpeI ACTAGT 1 cut(s) 1048
SphI GCATGC 1 cut(s) 516
Sse9I AATT 9 cut(s) 205, 241, 454, 462, 832, 930, 1130, 1225, 1380
SseBI AGGCCT 1 cut(s) 1396
SspI AATATT 2 cut(s) 891, 1462
StuI AGGCCT 1 cut(s) 1396
StyD4I CCNGG 3 cut(s) 115, 589, 791
StyI CCWWGG 3 cut(s) 598, 853, 1586
TaaI ACNGT 3 cut(s) 134, 845, 1471
TaiI ACGT 4 cut(s) 533, 1429, 1545, 1671
TaqI TCGA 1 cut(s) 729
TaqII GACCGA 1 cut(s) 965
TasI AATT 9 cut(s) 205, 241, 454, 462, 832, 930, 1130, 1225, 1380
TatI WGTACW 6 cut(s) 59, 209, 702, 723, 800, 879
Tru1I TTAA 5 cut(s) 240, 246, 963, 1505, 1511
Tru9I TTAA 5 cut(s) 240, 246, 963, 1505, 1511
TscAI CASTG 1 cut(s) 137
TseI GCWGC 4 cut(s) 120, 357, 935, 1148
TspDTI ATGAA 5 cut(s) 704, 1051, 1082, 1183, 1550
TspGWI ACGGA 1 cut(s) 518
TspRI CASTG 1 cut(s) 137
VpaK11BI GGWCC 1 cut(s) 672
VspI ATTAAT 1 cut(s) 240
XbaI TCTAGA 1 cut(s) 31
XceI RCATGY 1 cut(s) 516
XmiI GTMKAC 1 cut(s) 563
XmnI GAANNNNTTC 1 cut(s) 1428
ZrmI AGTACT 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.