Prupe.4G125600_v2.0.a1

Component of the DNA topoisomerase VI involved in chromatin organization and progression of endoreduplication cycles. Relaxes both positive and negative superturns and exhibits a strong decatenase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
N/A
Physical Location & Seq
Reverse (-)
6888222 .. 6888898
677 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G125600.1_v2.0.a1.CDS.2

Sequence Viewer

Length: 606 bp
ATGGCGGTCTTTATTGCGGGCGATCTTCGTTTCTGTCAATATGGCGTTGGTATGGTTGATTGTGCGAATGCCCGTGGGCAAGAGGTTACTCTGCACGATGTCGATAGCAGCGGTGGTGACATGACGAGCAACGCAGAGTACATAATACTGGTGGTTGGGAGTTACGGTGCTTACTTTCGATTGACCAAGGATGAGGTTGACCGTAGGTTTCCTTGTATCATTGTCTACACACCAGACCCCGTGCCCGAAGAGGATACGATCTCCTTTGTAAGGAAGATGAAGGAGGAGTTGGATCTCCCAGTGTTAGCCATTGCCGAGAGTAGTCCACGTGATGTGGTGGCGTTAAAAATGCATCCACGGTGTATGCGGCCCATGAATAGCAATAGCAAGGATCTTAAGCTAGCGCAACGTCTCTTGGAAAAAGATGCAATCGTGAAGGAACGTCCACAATGGGTTGAGGAGCTGACGCTAATGGTCGAGACCCGCCGCAAAGTAGAAGTTGACGCATTGTCCCCCTTAGGGAGGAGCTTCTTGTCCAACGTTTATTTTCCAAACAAGATTGGATATGACAATCTTCTAAATTATTACTCATATAAGCTACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04113 map04113
Pfam Domains
Protein Families
No domains found.

Protein Analysis

202

Amino Acids

22.85

Weight (kDa)

5.33

Isoelectric Point (pI)

41.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 225
AciI CCGC 6 cut(s) 5, 17, 111, 367, 484, 487
AclI AACGTT 1 cut(s) 540
AclWI GGATC 2 cut(s) 300, 399
AcvI CACGTG 1 cut(s) 329
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 3 cut(s) 270, 519, 522
AflII CTTAAG 1 cut(s) 395
AhdI GACNNNNNGTC 1 cut(s) 508
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AluBI AGCT 4 cut(s) 400, 463, 528, 598
AluI AGCT 4 cut(s) 400, 463, 528, 598
Alw26I GTCTC 2 cut(s) 416, 473
AlwI GGATC 2 cut(s) 300, 399
AoxI GGCC 1 cut(s) 368
ApeKI GCWGC 1 cut(s) 108
AspLEI GCGC 1 cut(s) 406
AspS9I GGNCC 1 cut(s) 369
AsuHPI GGTGA 1 cut(s) 128
AsuNHI GCTAGC 1 cut(s) 400
AxyI CCTNAGG 1 cut(s) 517
BaeGI GKGCMC 1 cut(s) 246
BbrPI CACGTG 1 cut(s) 329
BbvI GCAGC 1 cut(s) 120
BciVI GTATCC 1 cut(s) 247
BcoDI GTCTC 2 cut(s) 416, 473
BfaI CTAG 1 cut(s) 401
BfrI CTTAAG 1 cut(s) 395
BfuI GTATCC 1 cut(s) 247
BisI GCNGC 3 cut(s) 109, 368, 487
BlsI GCNGC 3 cut(s) 110, 369, 488
BmeRI GACNNNNNGTC 1 cut(s) 508
BmgT120I GGNCC 1 cut(s) 369
BmrI ACTGGG 1 cut(s) 293
BmsI GCATC 2 cut(s) 361, 415
BmtI GCTAGC 1 cut(s) 404
BmuI ACTGGG 1 cut(s) 293
BsaAI YACGTR 1 cut(s) 329
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 3 cut(s) 73, 186, 356
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bsc4I CCNNNNNNNGG 3 cut(s) 270, 519, 522
Bse1I ACTGG 2 cut(s) 153, 299
Bse21I CCTNAGG 1 cut(s) 517
Bse3DI GCAATG 1 cut(s) 309
BseDI CCNNGG 3 cut(s) 73, 186, 356
BseGI GGATG 2 cut(s) 196, 352
BseLI CCNNNNNNNGG 3 cut(s) 270, 519, 522
BseMI GCAATG 1 cut(s) 309
BseNI ACTGG 2 cut(s) 153, 299
BseRI GAGGAG 3 cut(s) 299, 473, 538
BseSI GKGCMC 1 cut(s) 246
BseXI GCAGC 1 cut(s) 120
BsgI GTGCAG 1 cut(s) 77
BshFI GGCC 1 cut(s) 370
BslFI GGGAC 1 cut(s) 496
BslI CCNNNNNNNGG 3 cut(s) 270, 519, 522
BsmAI GTCTC 2 cut(s) 416, 473
BsmBI CGTCTC 1 cut(s) 416
BsmFI GGGAC 1 cut(s) 496
BsmI GAATGC 1 cut(s) 73
BsnI GGCC 1 cut(s) 370
Bso31I GGTCTC 1 cut(s) 473
Bsp1286I GDGCHC 1 cut(s) 246
Bsp143I GATC 4 cut(s) 22, 258, 292, 391
BspACI CCGC 6 cut(s) 5, 17, 111, 367, 484, 487
BspANI GGCC 1 cut(s) 370
BspOI GCTAGC 1 cut(s) 404
BspPI GGATC 2 cut(s) 300, 399
BspTI CTTAAG 1 cut(s) 395
BspTNI GGTCTC 1 cut(s) 473
BsrDI GCAATG 1 cut(s) 309
BsrI ACTGG 2 cut(s) 153, 299
BssECI CCNNGG 3 cut(s) 73, 186, 356
BssMI GATC 4 cut(s) 22, 258, 292, 391
BssT1I CCWWGG 1 cut(s) 186
Bst4CI ACNGT 3 cut(s) 167, 203, 360
Bst6I CTCTTC 1 cut(s) 243
BstAFI CTTAAG 1 cut(s) 395
BstBAI YACGTR 1 cut(s) 329
BstC8I GCNNGC 2 cut(s) 19, 402
BstDEI CTNAG 1 cut(s) 517
BstDSI CCRYGG 2 cut(s) 73, 356
BstENI CCTNNNNNAGG 2 cut(s) 268, 520
BstF5I GGATG 2 cut(s) 196, 352
BstHHI GCGC 1 cut(s) 406
BstKTI GATC 4 cut(s) 25, 261, 295, 394
BstMAI GTCTC 2 cut(s) 416, 473
BstMBI GATC 4 cut(s) 22, 258, 292, 391
BstSLI GKGCMC 1 cut(s) 246
BstV1I GCAGC 1 cut(s) 120
BstX2I RGATCY 2 cut(s) 292, 391
BstYI RGATCY 2 cut(s) 292, 391
Bsu36I CCTNAGG 1 cut(s) 517
BsuI GTATCC 1 cut(s) 247
BsuRI GGCC 1 cut(s) 370
BtgI CCRYGG 2 cut(s) 73, 356
BtsCI GGATG 2 cut(s) 196, 352
BtsIMutI CAGTG 1 cut(s) 306
Cac8I GCNNGC 2 cut(s) 19, 402
CfoI GCGC 1 cut(s) 406
Cfr13I GGNCC 1 cut(s) 369
CseI GACGC 2 cut(s) 475, 512
Csp6I GTAC 1 cut(s) 139
CviAII CATG 2 cut(s) 121, 373
CviJI RGCY 6 cut(s) 308, 370, 400, 463, 528, 598
CviKI_1 RGCY 6 cut(s) 308, 370, 400, 463, 528, 598
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 1 cut(s) 517
DpnI GATC 4 cut(s) 24, 260, 294, 393
DpnII GATC 4 cut(s) 22, 258, 292, 391
DriI GACNNNNNGTC 1 cut(s) 508
Eam1104I CTCTTC 1 cut(s) 243
Eam1105I GACNNNNNGTC 1 cut(s) 508
EarI CTCTTC 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 186
Eco31I GGTCTC 1 cut(s) 473
Eco72I CACGTG 1 cut(s) 329
Eco81I CCTNAGG 1 cut(s) 517
EcoNI CCTNNNNNAGG 2 cut(s) 268, 520
EcoT14I CCWWGG 1 cut(s) 186
EcoT22I ATGCAT 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 186
Esp3I CGTCTC 1 cut(s) 416
FaeI CATG 2 cut(s) 124, 376
FaiI YATR 9 cut(s) 42, 53, 122, 143, 365, 374, 567, 592, 594
FaqI GGGAC 1 cut(s) 496
FatI CATG 2 cut(s) 120, 372
FauI CCCGC 2 cut(s) 10, 491
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 3 cut(s) 109, 368, 487
FokI GGATG 2 cut(s) 203, 339
Fsp4HI GCNGC 3 cut(s) 109, 368, 487
FspBI CTAG 1 cut(s) 401
GlaI GCGC 1 cut(s) 405
GluI GCNGC 3 cut(s) 109, 368, 487
HaeIII GGCC 1 cut(s) 370
HgaI GACGC 2 cut(s) 475, 512
HhaI GCGC 1 cut(s) 406
Hin1II CATG 2 cut(s) 124, 376
Hin6I GCGC 1 cut(s) 404
HinP1I GCGC 1 cut(s) 404
HincII GTYRAC 2 cut(s) 199, 502
HindII GTYRAC 2 cut(s) 199, 502
HphI GGTGA 1 cut(s) 128
Hpy166II GTNNAC 5 cut(s) 199, 226, 326, 446, 502
Hpy188III TCNNGA 2 cut(s) 433, 478
Hpy8I GTNNAC 5 cut(s) 199, 226, 326, 446, 502
HpyAV CCTTC 2 cut(s) 274, 430
HpyCH4III ACNGT 3 cut(s) 167, 203, 360
HpyCH4IV ACGT 4 cut(s) 328, 409, 442, 540
HpyCH4V TGCA 3 cut(s) 94, 352, 428
HpyF3I CTNAG 1 cut(s) 517
HpySE526I ACGT 4 cut(s) 328, 409, 442, 540
Hsp92II CATG 2 cut(s) 124, 376
HspAI GCGC 1 cut(s) 404
Kzo9I GATC 4 cut(s) 22, 258, 292, 391
LmnI GCTCC 2 cut(s) 460, 525
LpnPI CCDG 3 cut(s) 134, 246, 312
Lsp1109I GCAGC 1 cut(s) 120
LweI GCATC 2 cut(s) 361, 415
MaeI CTAG 1 cut(s) 401
MaeII ACGT 4 cut(s) 328, 409, 442, 540
MaeIII GTNAC 3 cut(s) 85, 116, 161
MalI GATC 4 cut(s) 24, 260, 294, 393
MboI GATC 4 cut(s) 22, 258, 292, 391
MboII GAAGA 4 cut(s) 17, 260, 286, 566
MflI RGATCY 2 cut(s) 292, 391
MhlI GDGCHC 1 cut(s) 246
MluCI AATT 1 cut(s) 580
MmeI TCCRAC 2 cut(s) 270, 561
MnlI CCTC 6 cut(s) 76, 187, 244, 277, 451, 516
Mph1103I ATGCAT 1 cut(s) 354
MseI TTAA 3 cut(s) 344, 396, 604
MspA1I CMGCKG 1 cut(s) 111
MspCI CTTAAG 1 cut(s) 395
Mva1269I GAATGC 1 cut(s) 73
NdeII GATC 4 cut(s) 22, 258, 292, 391
NheI GCTAGC 1 cut(s) 400
NlaIII CATG 2 cut(s) 124, 376
NmeAIII GCCGAG 1 cut(s) 340
NmuCI GTSAC 1 cut(s) 116
NsiI ATGCAT 1 cut(s) 354
PctI GAATGC 1 cut(s) 73
PkrI GCNGC 3 cut(s) 110, 369, 488
PmaCI CACGTG 1 cut(s) 329
PmlI CACGTG 1 cut(s) 329
Ppu21I YACGTR 1 cut(s) 329
Psp1406I AACGTT 1 cut(s) 540
PspCI CACGTG 1 cut(s) 329
PspPI GGNCC 1 cut(s) 369
PsuI RGATCY 2 cut(s) 292, 391
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
SaqAI TTAA 3 cut(s) 344, 396, 604
SatI GCNGC 3 cut(s) 109, 368, 487
Sau3AI GATC 4 cut(s) 22, 258, 292, 391
Sau96I GGNCC 1 cut(s) 369
SduI GDGCHC 1 cut(s) 246
SfaNI GCATC 2 cut(s) 361, 415
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
Sse9I AATT 1 cut(s) 580
SsiI CCGC 6 cut(s) 5, 17, 111, 367, 484, 487
SspMI CTAG 1 cut(s) 401
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 3 cut(s) 167, 203, 360
TaiI ACGT 4 cut(s) 331, 412, 445, 543
TaqI TCGA 3 cut(s) 102, 178, 477
TasI AATT 1 cut(s) 580
TatI WGTACW 1 cut(s) 138
TauI GCSGC 2 cut(s) 370, 489
Tru1I TTAA 3 cut(s) 344, 396, 604
Tru9I TTAA 3 cut(s) 344, 396, 604
TscAI CASTG 1 cut(s) 306
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 1 cut(s) 108
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 2 cut(s) 293, 389
TspRI CASTG 1 cut(s) 306
Vha464I CTTAAG 1 cut(s) 395
XagI CCTNNNNNAGG 2 cut(s) 268, 520
XmiI GTMKAC 1 cut(s) 225
XspI CTAG 1 cut(s) 401
Zsp2I ATGCAT 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.