Prupe.4G133000_v2.0.a1

Phosphodiesterase responsible for the U6 snRNA 3' end processing. Acts as an exoribonuclease (RNase) responsible for trimming the poly(U) tract of the last nucleotides in the pre-U6 snRNA molecule, leading to the formation of mature U6 snRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
N/A
Physical Location & Seq
Reverse (-)
7370011 .. 7370936
926 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G133000.1_v2.0.a1.CDS.2

Sequence Viewer

Length: 594 bp
ATGGGGAAAGAGAAGTTTTTGGGTGTCTTGAGAAGGGTTGGCTGGGGTTCTGGGTTCGATGGGAAAGCAAAGAGAGCCTTTAATCCCTTGATTAATTGTATATTTGCAGTTCATATACCACCAGCACCAGGGAAAGAGATGGCCTTATTTTTGAACAAGCTAGCTTCTATGATTTTGCGCAAGGATGAGCACAAGCTTGAACGGGTTGCTTTGGGTAGAGAGTTCCTTATAAGTCTGGGAAAATCTGTACCAATCCGAGTGCACCAGGTTGACTCCCTGGTCACAATGCTTCGGCAGAAGCTTCAGATTCAGAGGCGGTATGAACATAATTCTCTCGCGTGCATGATTTTTACTTCTTTTATTGAACATAATGCTGATTTGCGCAAGTGGGAGGTTTTTGTTAACAATGATCACACCCGGACCTTTATGTCGATAGAAGTTATTGCTGCCGGGTTAGCTGAGATAACAAAGCAGATTCAAGCTGTCAATGAGGTGTATAAGCTTCACAATCTTCCTGAATTTTACAAGGATCCAGGCCCTCATAACTCGGAAAAACAGAGGCCTAAAGTCCATCATTATCAGGGAACCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HVSL

Protein Analysis

198

Amino Acids

22.64

Weight (kDa)

9.75

Isoelectric Point (pI)

52.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 230
AasI GACNNNNNNGTC 2 cut(s) 278, 427
Acc16I TGCGCA 2 cut(s) 179, 383
AccII CGCG 1 cut(s) 338
AciI CCGC 1 cut(s) 316
AclWI GGATC 2 cut(s) 524, 537
AcsI RAATTY 1 cut(s) 518
AcuI CTGAAG 1 cut(s) 287
AfaI GTAC 1 cut(s) 249
AfiI CCNNNNNNNGG 1 cut(s) 128
AgsI TTSAA 4 cut(s) 154, 200, 365, 479
AjnI CCWGG 4 cut(s) 127, 264, 276, 532
AluBI AGCT 7 cut(s) 160, 164, 196, 301, 458, 482, 502
AluI AGCT 7 cut(s) 160, 164, 196, 301, 458, 482, 502
Alw21I GWGCWC 2 cut(s) 192, 264
Alw44I GTGCAC 1 cut(s) 260
AlwI GGATC 2 cut(s) 524, 537
AoxI GGCC 3 cut(s) 141, 535, 560
ApaLI GTGCAC 1 cut(s) 260
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 1 cut(s) 518
AseI ATTAAT 1 cut(s) 93
AspLEI GCGC 2 cut(s) 180, 384
AspS9I GGNCC 2 cut(s) 420, 536
AsuC2I CCSGG 2 cut(s) 418, 451
AsuNHI GCTAGC 1 cut(s) 160
AvaII GGWCC 1 cut(s) 420
BaeGI GKGCMC 1 cut(s) 264
BamHI GGATCC 1 cut(s) 529
Bbv12I GWGCWC 2 cut(s) 192, 264
BbvI GCAGC 1 cut(s) 433
BccI CCATC 3 cut(s) 53, 133, 579
BciT130I CCWGG 4 cut(s) 129, 266, 278, 534
BclI TGATCA 1 cut(s) 409
BcnI CCSGG 2 cut(s) 418, 451
BfaI CTAG 1 cut(s) 161
BisI GCNGC 1 cut(s) 447
BlsI GCNGC 1 cut(s) 448
Bme1390I CCNGG 6 cut(s) 129, 266, 278, 418, 451, 534
Bme18I GGWCC 1 cut(s) 420
BmgT120I GGNCC 2 cut(s) 420, 536
BmiI GGNNCC 2 cut(s) 531, 586
BmrFI CCNGG 6 cut(s) 129, 266, 278, 418, 451, 534
BmtI GCTAGC 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 49
BpuMI CCSGG 2 cut(s) 418, 451
BsaJI CCNNGG 2 cut(s) 128, 276
Bsc4I CCNNNNNNNGG 1 cut(s) 128
BseBI CCWGG 4 cut(s) 129, 266, 278, 534
BseDI CCNNGG 2 cut(s) 128, 276
BseGI GGATG 1 cut(s) 190
BseLI CCNNNNNNNGG 1 cut(s) 128
BseMII CTCAG 1 cut(s) 450
BseSI GKGCMC 1 cut(s) 264
BseXI GCAGC 1 cut(s) 433
BseYI CCCAGC 1 cut(s) 42
Bsh1236I CGCG 1 cut(s) 338
BshFI GGCC 3 cut(s) 143, 537, 562
BsiHKAI GWGCWC 2 cut(s) 192, 264
BsiSI CCGG 2 cut(s) 418, 450
BslI CCNNNNNNNGG 1 cut(s) 128
BsnI GGCC 3 cut(s) 143, 537, 562
Bsp1286I GDGCHC 2 cut(s) 192, 264
Bsp143I GATC 2 cut(s) 409, 529
BspACI CCGC 1 cut(s) 316
BspANI GGCC 3 cut(s) 143, 537, 562
BspCNI CTCAG 1 cut(s) 451
BspFNI CGCG 1 cut(s) 338
BspLI GGNNCC 2 cut(s) 531, 586
BspOI GCTAGC 1 cut(s) 164
BspPI GGATC 2 cut(s) 524, 537
BssECI CCNNGG 2 cut(s) 128, 276
BssMI GATC 2 cut(s) 409, 529
Bst2UI CCWGG 4 cut(s) 129, 266, 278, 534
BstC8I GCNNGC 2 cut(s) 162, 340
BstDEI CTNAG 1 cut(s) 459
BstF5I GGATG 1 cut(s) 190
BstFNI CGCG 1 cut(s) 338
BstHHI GCGC 2 cut(s) 180, 384
BstKTI GATC 2 cut(s) 412, 532
BstMBI GATC 2 cut(s) 409, 529
BstMWI GCNNNNNNNGC 2 cut(s) 74, 455
BstNI CCWGG 4 cut(s) 129, 266, 278, 534
BstSCI CCNGG 6 cut(s) 127, 264, 276, 416, 449, 532
BstSLI GKGCMC 1 cut(s) 264
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 1 cut(s) 433
BstX2I RGATCY 1 cut(s) 529
BstYI RGATCY 1 cut(s) 529
BsuRI GGCC 3 cut(s) 143, 537, 562
BtsCI GGATG 1 cut(s) 190
Cac8I GCNNGC 2 cut(s) 162, 340
CfoI GCGC 2 cut(s) 180, 384
Cfr13I GGNCC 2 cut(s) 420, 536
CsiI ACCWGGT 1 cut(s) 264
Csp6I GTAC 1 cut(s) 248
CviAII CATG 1 cut(s) 343
CviQI GTAC 1 cut(s) 248
DdeI CTNAG 1 cut(s) 459
DpnI GATC 2 cut(s) 411, 531
DpnII GATC 2 cut(s) 409, 529
DrdI GACNNNNNNGTC 2 cut(s) 278, 427
DseDI GACNNNNNNGTC 2 cut(s) 278, 427
Eco147I AGGCCT 1 cut(s) 562
Eco47I GGWCC 1 cut(s) 420
Eco57I CTGAAG 1 cut(s) 287
EcoO109I RGGNCCY 1 cut(s) 536
EcoRII CCWGG 4 cut(s) 127, 264, 276, 532
FaeI CATG 1 cut(s) 346
FalI AAGNNNNNCTT 2 cut(s) 62, 94
FatI CATG 1 cut(s) 342
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 1 cut(s) 447
FokI GGATG 1 cut(s) 197
Fsp4HI GCNGC 1 cut(s) 447
FspBI CTAG 1 cut(s) 161
FspI TGCGCA 2 cut(s) 179, 383
GlaI GCGC 2 cut(s) 179, 383
GluI GCNGC 1 cut(s) 447
GsaI CCCAGC 1 cut(s) 46
HaeIII GGCC 3 cut(s) 143, 537, 562
HapII CCGG 2 cut(s) 418, 450
HhaI GCGC 2 cut(s) 180, 384
Hin1II CATG 1 cut(s) 346
Hin6I GCGC 2 cut(s) 178, 382
HinP1I GCGC 2 cut(s) 178, 382
HincII GTYRAC 2 cut(s) 271, 403
HindII GTYRAC 2 cut(s) 271, 403
HindIII AAGCTT 3 cut(s) 194, 299, 500
HinfI GANTC 3 cut(s) 272, 307, 475
HpaI GTTAAC 1 cut(s) 403
HpaII CCGG 2 cut(s) 418, 450
Hpy166II GTNNAC 3 cut(s) 262, 271, 403
Hpy188I TCNGA 4 cut(s) 257, 306, 312, 550
Hpy188III TCNNGA 2 cut(s) 28, 515
Hpy8I GTNNAC 3 cut(s) 262, 271, 403
HpyAV CCTTC 1 cut(s) 27
HpyCH4V TGCA 3 cut(s) 107, 262, 342
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 455
HpyF3I CTNAG 1 cut(s) 459
Hsp92II CATG 1 cut(s) 346
HspAI GCGC 2 cut(s) 178, 382
Ksp22I TGATCA 1 cut(s) 409
KspAI GTTAAC 1 cut(s) 403
Kzo9I GATC 2 cut(s) 409, 529
Lsp1109I GCAGC 1 cut(s) 433
MabI ACCWGGT 1 cut(s) 264
MaeI CTAG 1 cut(s) 161
MaeIII GTNAC 1 cut(s) 280
MalI GATC 2 cut(s) 411, 531
MboI GATC 2 cut(s) 409, 529
MboII GAAGA 1 cut(s) 503
MfeI CAATTG 1 cut(s) 589
MflI RGATCY 1 cut(s) 529
MhlI GDGCHC 2 cut(s) 192, 264
MluCI AATT 4 cut(s) 94, 328, 518, 589
MlyI GAGTC 1 cut(s) 266
MnlI CCTC 5 cut(s) 306, 385, 484, 549, 552
MseI TTAA 3 cut(s) 81, 93, 402
MspI CCGG 2 cut(s) 418, 450
MspR9I CCNGG 6 cut(s) 129, 266, 278, 418, 451, 534
MunI CAATTG 1 cut(s) 589
MvaI CCWGG 4 cut(s) 129, 266, 278, 534
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 2 cut(s) 74, 455
NciI CCSGG 2 cut(s) 418, 451
NdeII GATC 2 cut(s) 409, 529
NheI GCTAGC 1 cut(s) 160
NlaIII CATG 1 cut(s) 346
NlaIV GGNNCC 2 cut(s) 531, 586
NmuCI GTSAC 1 cut(s) 280
NsbI TGCGCA 2 cut(s) 179, 383
PceI AGGCCT 1 cut(s) 562
PfeI GAWTC 2 cut(s) 307, 475
PkrI GCNGC 1 cut(s) 448
PleI GAGTC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 266
PshBI ATTAAT 1 cut(s) 93
PsiI TTATAA 1 cut(s) 230
Psp6I CCWGG 4 cut(s) 127, 264, 276, 532
PspFI CCCAGC 1 cut(s) 42
PspGI CCWGG 4 cut(s) 127, 264, 276, 532
PspN4I GGNNCC 2 cut(s) 531, 586
PspPI GGNCC 2 cut(s) 420, 536
PsuI RGATCY 1 cut(s) 529
RsaI GTAC 1 cut(s) 249
RsaNI GTAC 1 cut(s) 248
SaqAI TTAA 3 cut(s) 81, 93, 402
SatI GCNGC 1 cut(s) 447
Sau3AI GATC 2 cut(s) 409, 529
Sau96I GGNCC 2 cut(s) 420, 536
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 6 cut(s) 129, 266, 278, 418, 451, 534
SduI GDGCHC 2 cut(s) 192, 264
SexAI ACCWGGT 1 cut(s) 264
SinI GGWCC 1 cut(s) 420
SmlI CTYRAG 1 cut(s) 28
SmoI CTYRAG 1 cut(s) 28
Sse9I AATT 4 cut(s) 94, 328, 518, 589
SseBI AGGCCT 1 cut(s) 562
SsiI CCGC 1 cut(s) 316
SspMI CTAG 1 cut(s) 161
StuI AGGCCT 1 cut(s) 562
StyD4I CCNGG 6 cut(s) 127, 264, 276, 416, 449, 532
TaqI TCGA 2 cut(s) 57, 431
TasI AATT 4 cut(s) 94, 328, 518, 589
TfiI GAWTC 2 cut(s) 307, 475
Tru1I TTAA 3 cut(s) 81, 93, 402
Tru9I TTAA 3 cut(s) 81, 93, 402
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 1 cut(s) 446
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 2 cut(s) 101, 336
VneI GTGCAC 1 cut(s) 260
VpaK11BI GGWCC 1 cut(s) 420
VspI ATTAAT 1 cut(s) 93
XapI RAATTY 1 cut(s) 518
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.