Prupe.4G142100_v2.0.a1

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
N/A
Physical Location & Seq
Reverse (-)
8157896 .. 8159708
1813 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G142100.1_v2.0.a1.CDS.3

Sequence Viewer

Length: 1299 bp
ATGAACGTTGAAGTGGAAATAATCTCCAAGGAGATCATCAAACCATTTTCTCCAACCCCTAAACATCTTCGACGCCATCAGTTTTCCTTTCTTGATCAAATAAATCCCCCAATTTATTCCTCCTTGAAGTCCTTATCCGAGGTCTTGACCCTTTTCTACCCACTAGCCGGCCGACTCAGAGTCGAGAACCATTTTGTAGACTGCAATGACGAGGGAATACCCTACCTTGAAGGTCAAGTAAAACCCAATTGCCAGCTGTGTGATTTTCTCAATGATCCGGTCCCTGATGACCTCCAGAAATTTGTCCCCTTTGAGGCACATGATCATAATGAATTTTCCCTAGGTGTCCAGCTCAACATGTTCGAATGTGGAGGCTTCGCCATTGGTCTAAGCGTTTCTCACAAGCTCGCAGATGGACTATCTATGCTCATGCCCACCAAAACTTGGGCTGCCATTTCCCGCGGAGAATATGATAATCTTGAAGCCAAAATAGAGCATCCGGAATTTGTTTCAGCCACACTCTTCCCACCCAAGGAGATGACCAGGTACTACTCGCACTCCGGTATCACCAAGAACAAAGTAACAAAGAGGTTCGTGTTCGATTCCTCTGCGATAGAGGACCTCAGAGAAAAATATACAAGCTTGGAAAACAATGAGAAACGCCCGTCACGTGTTGAGACCTTGTCGGCTTTCATATGGAGGCGATTTGTGGAAGCTACCAAGGCTGATCCTGATCAAAATATTGATGATAAGAATAAGTTGCATATGGTGATCCAGACTGTGAACCTGCGCCCCAGGATTGATCCACCATTGCAACAAAATTCATTTGGAAATATTTGTCATTTTTTCATGATTAGTAGTAGTGGGGATGATGATTATGATGATGAAGATGAAGAATCTTGTCACGGCATGGTGAGGCGTGTACGAGAAGAAATAAGTAAGATTGACAAGGACTACCTGAAAAGGCTACAACAGGGTGATGAGCACTTCAAATTCATGAACCGACTTGCTCTAACTTCTATTAGATCTGCAGGGAAGGTAGTTACGTCTTACTTCAGTAGTTTGTGCAGATTTCCCCTCTATGATATGGATTTTGGTTGGGGGAGACCGGCATGGGTGGGCATACAGCCACTACCCTTGAACAACCTAATAGTTTTCGTCGACACCAAAGAGACTGGCGGAATAGAGGCATACGTTACCCTGGCGGATCAGCATGTCATGGCTAAATTCGAAACAACAAAGAAGGATTTTTTGTTGTTGAGTTGTGCTACCTCCCACTTGACATTGAAAAACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

433

Amino Acids

49.58

Weight (kDa)

5.73

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 795
AccB7I CCANNNNNTGG 1 cut(s) 444
AccI GTMKAC 2 cut(s) 198, 1161
AccII CGCG 1 cut(s) 462
AccIII TCCGGA 1 cut(s) 499
AciI CCGC 4 cut(s) 460, 462, 1179, 1205
AclI AACGTT 1 cut(s) 6
AclWI GGATC 5 cut(s) 269, 722, 766, 797, 1215
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 6 cut(s) 299, 332, 503, 820, 992, 1226
AcuI CTGAAG 1 cut(s) 1039
AcvI CACGTG 1 cut(s) 671
AcyI GRCGYC 1 cut(s) 73
AfaI GTAC 2 cut(s) 548, 924
AfiI CCNNNNNNNGG 4 cut(s) 167, 313, 444, 532
AflIII ACRYGT 2 cut(s) 357, 670
AgsI TTSAA 7 cut(s) 11, 127, 230, 482, 991, 1141, 1288
AhdI GACNNNNNGTC 1 cut(s) 179
AjnI CCWGG 3 cut(s) 542, 794, 1200
AluBI AGCT 5 cut(s) 256, 352, 406, 642, 716
AluI AGCT 5 cut(s) 256, 352, 406, 642, 716
Alw21I GWGCWC 1 cut(s) 987
Alw26I GTCTC 3 cut(s) 671, 1099, 1166
AlwI GGATC 5 cut(s) 269, 722, 766, 797, 1215
Aor13HI TCCGGA 1 cut(s) 499
AoxI GGCC 1 cut(s) 169
ApeKI GCWGC 1 cut(s) 449
ApoI RAATTY 6 cut(s) 299, 332, 503, 820, 992, 1226
AspA2I CCTAGG 1 cut(s) 340
AspLEI GCGC 1 cut(s) 792
AspS9I GGNCC 2 cut(s) 280, 619
AsuHPI GGTGA 4 cut(s) 559, 781, 925, 989
AsuII TTCGAA 2 cut(s) 363, 1230
AvaII GGWCC 2 cut(s) 280, 619
AvrII CCTAGG 1 cut(s) 340
BaeI ACNNNNGTAYC 2 cut(s) 547, 580
BbrPI CACGTG 1 cut(s) 671
Bbv12I GWGCWC 1 cut(s) 987
BbvI GCAGC 1 cut(s) 436
BccI CCATC 2 cut(s) 84, 407
BceAI ACGGC 1 cut(s) 922
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 3 cut(s) 544, 796, 1202
BclI TGATCA 3 cut(s) 94, 322, 733
BcoDI GTCTC 3 cut(s) 671, 1099, 1166
BfaI CTAG 2 cut(s) 164, 341
BfmI CTRYAG 1 cut(s) 1029
BfuAI ACCTGC 1 cut(s) 795
BglII AGATCT 1 cut(s) 1025
BisI GCNGC 1 cut(s) 450
BlnI CCTAGG 1 cut(s) 340
BlsI GCNGC 1 cut(s) 451
Bme1390I CCNGG 3 cut(s) 544, 796, 1202
Bme18I GGWCC 2 cut(s) 280, 619
BmeRI GACNNNNNGTC 1 cut(s) 179
BmgT120I GGNCC 2 cut(s) 280, 619
BmiI GGNNCC 1 cut(s) 282
BmrFI CCNGG 3 cut(s) 544, 796, 1202
BmsI GCATC 1 cut(s) 505
BpmI CTGGAG 1 cut(s) 278
Bpu14I TTCGAA 2 cut(s) 363, 1230
BsaAI YACGTR 1 cut(s) 671
BsaBI GATNNNNATC 1 cut(s) 732
BsaHI GRCGYC 1 cut(s) 73
BsaI GGTCTC 2 cut(s) 671, 1099
BsaJI CCNNGG 8 cut(s) 27, 138, 340, 460, 531, 720, 794, 1200
BsaWI WCCGGW 3 cut(s) 277, 499, 560
BsaXI ACNNNNNCTCC 2 cut(s) 542, 572
Bsc4I CCNNNNNNNGG 4 cut(s) 167, 313, 444, 532
Bse118I RCCGGY 2 cut(s) 167, 1108
Bse1I ACTGG 1 cut(s) 1180
Bse3DI GCAATG 2 cut(s) 211, 809
Bse8I GATNNNNATC 1 cut(s) 732
BseAI TCCGGA 1 cut(s) 499
BseBI CCWGG 3 cut(s) 544, 796, 1202
BseDI CCNNGG 8 cut(s) 27, 138, 340, 460, 531, 720, 794, 1200
BseGI GGATG 2 cut(s) 496, 874
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 4 cut(s) 167, 313, 444, 532
BseMI GCAATG 2 cut(s) 211, 809
BseMII CTCAG 2 cut(s) 190, 637
BseNI ACTGG 1 cut(s) 1180
BseX3I CGGCCG 1 cut(s) 169
BseXI GCAGC 1 cut(s) 436
BsgI GTGCAG 1 cut(s) 1087
Bsh1236I CGCG 1 cut(s) 462
Bsh1285I CGRYCG 1 cut(s) 172
BshFI GGCC 1 cut(s) 171
BsiEI CGRYCG 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 987
BsiSI CCGG 5 cut(s) 168, 278, 500, 561, 1109
BslFI GGGAC 2 cut(s) 266, 290
BslI CCNNNNNNNGG 4 cut(s) 167, 313, 444, 532
BsmAI GTCTC 3 cut(s) 671, 1099, 1166
BsmFI GGGAC 2 cut(s) 266, 290
BsnI GGCC 1 cut(s) 171
Bso31I GGTCTC 2 cut(s) 671, 1099
Bsp119I TTCGAA 2 cut(s) 363, 1230
Bsp1286I GDGCHC 1 cut(s) 987
Bsp13I TCCGGA 1 cut(s) 499
BspACI CCGC 4 cut(s) 460, 462, 1179, 1205
BspANI GGCC 1 cut(s) 171
BspCNI CTCAG 2 cut(s) 189, 636
BspEI TCCGGA 1 cut(s) 499
BspFNI CGCG 1 cut(s) 462
BspHI TCATGA 2 cut(s) 849, 996
BspLI GGNNCC 1 cut(s) 282
BspMAI CTGCAG 1 cut(s) 1033
BspMI ACCTGC 1 cut(s) 795
BspPI GGATC 5 cut(s) 269, 722, 766, 797, 1215
BspT104I TTCGAA 2 cut(s) 363, 1230
BspTNI GGTCTC 2 cut(s) 671, 1099
BsrDI GCAATG 2 cut(s) 211, 809
BsrFI RCCGGY 2 cut(s) 167, 1108
BsrI ACTGG 1 cut(s) 1180
BssAI RCCGGY 2 cut(s) 167, 1108
BssECI CCNNGG 8 cut(s) 27, 138, 340, 460, 531, 720, 794, 1200
BssNI GRCGYC 1 cut(s) 73
BssT1I CCWWGG 4 cut(s) 27, 340, 531, 720
Bst2UI CCWGG 3 cut(s) 544, 796, 1202
Bst4CI ACNGT 1 cut(s) 781
Bst6I CTCTTC 1 cut(s) 527
BstACI GRCGYC 1 cut(s) 73
BstBAI YACGTR 1 cut(s) 671
BstBI TTCGAA 2 cut(s) 363, 1230
BstC8I GCNNGC 3 cut(s) 169, 254, 408
BstDEI CTNAG 3 cut(s) 176, 389, 623
BstDSI CCRYGG 1 cut(s) 460
BstF5I GGATG 2 cut(s) 496, 874
BstFNI CGCG 1 cut(s) 462
BstHHI GCGC 1 cut(s) 792
BstMAI GTCTC 3 cut(s) 671, 1099, 1166
BstMCI CGRYCG 1 cut(s) 172
BstMWI GCNNNNNNNGC 1 cut(s) 722
BstNI CCWGG 3 cut(s) 544, 796, 1202
BstNSI RCATGY 2 cut(s) 361, 1217
BstSCI CCNGG 3 cut(s) 542, 794, 1200
BstSFI CTRYAG 1 cut(s) 1029
BstUI CGCG 1 cut(s) 462
BstV1I GCAGC 1 cut(s) 436
BstX2I RGATCY 1 cut(s) 1025
BstYI RGATCY 1 cut(s) 1025
BstZI CGGCCG 1 cut(s) 169
BsuRI GGCC 1 cut(s) 171
BtgI CCRYGG 1 cut(s) 460
BtsCI GGATG 2 cut(s) 496, 874
BveI ACCTGC 1 cut(s) 795
Cac8I GCNNGC 3 cut(s) 169, 254, 408
CciI TCATGA 2 cut(s) 849, 996
CfoI GCGC 1 cut(s) 792
Cfr10I RCCGGY 2 cut(s) 167, 1108
Cfr13I GGNCC 2 cut(s) 280, 619
Cfr42I CCGCGG 1 cut(s) 463
CseI GACGC 1 cut(s) 81
CsiI ACCWGGT 1 cut(s) 542
Csp6I GTAC 2 cut(s) 547, 923
CspCI CAANNNNNGTGG 2 cut(s) 1119, 1154
CviAII CATG 9 cut(s) 320, 358, 430, 850, 910, 997, 1113, 1214, 1219
CviQI GTAC 2 cut(s) 547, 923
DdeI CTNAG 3 cut(s) 176, 389, 623
DriI GACNNNNNGTC 1 cut(s) 179
EaeI YGGCCR 1 cut(s) 169
EagI CGGCCG 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 527
Eam1105I GACNNNNNGTC 1 cut(s) 179
EarI CTCTTC 1 cut(s) 527
EciI GGCGGA 2 cut(s) 1194, 1220
EclXI CGGCCG 1 cut(s) 169
Eco130I CCWWGG 4 cut(s) 27, 340, 531, 720
Eco31I GGTCTC 2 cut(s) 671, 1099
Eco47I GGWCC 2 cut(s) 280, 619
Eco52I CGGCCG 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 1039
Eco72I CACGTG 1 cut(s) 671
EcoO109I RGGNCCY 1 cut(s) 619
EcoRII CCWGG 3 cut(s) 542, 794, 1200
EcoT14I CCWWGG 4 cut(s) 27, 340, 531, 720
ErhI CCWWGG 4 cut(s) 27, 340, 531, 720
FaeI CATG 9 cut(s) 323, 361, 433, 853, 913, 1000, 1116, 1217, 1222
FaqI GGGAC 2 cut(s) 266, 290
FatI CATG 9 cut(s) 319, 357, 429, 849, 909, 996, 1112, 1213, 1218
FauI CCCGC 1 cut(s) 467
FauNDI CATATG 2 cut(s) 695, 765
FbaI TGATCA 3 cut(s) 94, 322, 733
FblI GTMKAC 2 cut(s) 198, 1161
Fnu4HI GCNGC 1 cut(s) 450
FokI GGATG 2 cut(s) 483, 881
Fsp4HI GCNGC 1 cut(s) 450
FspBI CTAG 2 cut(s) 164, 341
GlaI GCGC 1 cut(s) 791
GluI GCNGC 1 cut(s) 450
GsuI CTGGAG 1 cut(s) 278
HaeIII GGCC 1 cut(s) 171
HapII CCGG 5 cut(s) 168, 278, 500, 561, 1109
HgaI GACGC 1 cut(s) 81
HhaI GCGC 1 cut(s) 792
Hin1I GRCGYC 1 cut(s) 73
Hin1II CATG 9 cut(s) 323, 361, 433, 853, 913, 1000, 1116, 1217, 1222
Hin6I GCGC 1 cut(s) 790
HinP1I GCGC 1 cut(s) 790
HincII GTYRAC 1 cut(s) 1162
HindII GTYRAC 1 cut(s) 1162
HindIII AAGCTT 1 cut(s) 640
HinfI GANTC 4 cut(s) 174, 180, 602, 896
HpaII CCGG 5 cut(s) 168, 278, 500, 561, 1109
HphI GGTGA 4 cut(s) 559, 781, 925, 989
Hpy166II GTNNAC 4 cut(s) 199, 784, 923, 1162
Hpy188I TCNGA 3 cut(s) 139, 179, 626
Hpy8I GTNNAC 4 cut(s) 199, 784, 923, 1162
Hpy99I CGWCG 2 cut(s) 75, 1163
HpyAV CCTTC 3 cut(s) 224, 1030, 1237
HpyCH4III ACNGT 1 cut(s) 781
HpyCH4IV ACGT 4 cut(s) 6, 670, 1046, 1194
HpyCH4V TGCA 5 cut(s) 204, 763, 814, 1031, 1068
HpyF10VI GCNNNNNNNGC 1 cut(s) 722
HpyF3I CTNAG 3 cut(s) 176, 389, 623
HpySE526I ACGT 4 cut(s) 6, 670, 1046, 1194
Hsp92I GRCGYC 1 cut(s) 73
Hsp92II CATG 9 cut(s) 323, 361, 433, 853, 913, 1000, 1116, 1217, 1222
HspAI GCGC 1 cut(s) 790
Kpn2I TCCGGA 1 cut(s) 499
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Ksp22I TGATCA 3 cut(s) 94, 322, 733
KspI CCGCGG 1 cut(s) 463
Lsp1109I GCAGC 1 cut(s) 436
LweI GCATC 1 cut(s) 505
MabI ACCWGGT 1 cut(s) 542
MaeI CTAG 2 cut(s) 164, 341
MaeII ACGT 4 cut(s) 6, 670, 1046, 1194
MaeIII GTNAC 5 cut(s) 580, 666, 902, 1042, 1195
MboII GAAGA 5 cut(s) 59, 514, 899, 905, 941
MfeI CAATTG 1 cut(s) 247
MflI RGATCY 1 cut(s) 1025
MhlI GDGCHC 1 cut(s) 987
MluCI AATT 8 cut(s) 111, 247, 299, 332, 503, 820, 992, 1226
MlyI GAGTC 2 cut(s) 168, 189
MmeI TCCRAC 1 cut(s) 77
MroI TCCGGA 1 cut(s) 499
MroNI GCCGGC 1 cut(s) 167
MspA1I CMGCKG 2 cut(s) 256, 462
MspI CCGG 5 cut(s) 168, 278, 500, 561, 1109
MspR9I CCNGG 3 cut(s) 544, 796, 1202
MunI CAATTG 1 cut(s) 247
MvaI CCWGG 3 cut(s) 544, 796, 1202
MvnI CGCG 1 cut(s) 462
MwoI GCNNNNNNNGC 1 cut(s) 722
NaeI GCCGGC 1 cut(s) 169
NdeI CATATG 2 cut(s) 695, 765
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 9 cut(s) 323, 361, 433, 853, 913, 1000, 1116, 1217, 1222
NlaIV GGNNCC 1 cut(s) 282
NmuCI GTSAC 2 cut(s) 666, 902
NspI RCATGY 2 cut(s) 361, 1217
NspV TTCGAA 2 cut(s) 363, 1230
PagI TCATGA 2 cut(s) 849, 996
PciI ACATGT 1 cut(s) 357
PcsI WCGNNNNNNNCGW 1 cut(s) 667
PdiI GCCGGC 1 cut(s) 169
PfeI GAWTC 2 cut(s) 602, 896
PflFI GACNNNGTC 1 cut(s) 682
PflMI CCANNNNNTGG 1 cut(s) 444
PkrI GCNGC 1 cut(s) 451
PleI GAGTC 2 cut(s) 168, 188
PmaCI CACGTG 1 cut(s) 671
PmlI CACGTG 1 cut(s) 671
PpsI GAGTC 2 cut(s) 168, 188
Ppu21I YACGTR 1 cut(s) 671
PpuMI RGGWCCY 1 cut(s) 619
PscI ACATGT 1 cut(s) 357
Psp1406I AACGTT 1 cut(s) 6
Psp5II RGGWCCY 1 cut(s) 619
Psp6I CCWGG 3 cut(s) 542, 794, 1200
PspCI CACGTG 1 cut(s) 671
PspGI CCWGG 3 cut(s) 542, 794, 1200
PspN4I GGNNCC 1 cut(s) 282
PspPI GGNCC 2 cut(s) 280, 619
PspPPI RGGWCCY 1 cut(s) 619
PstI CTGCAG 1 cut(s) 1033
PsuI RGATCY 1 cut(s) 1025
PsyI GACNNNGTC 1 cut(s) 682
PvuII CAGCTG 1 cut(s) 256
RsaI GTAC 2 cut(s) 548, 924
RsaNI GTAC 2 cut(s) 547, 923
SacII CCGCGG 1 cut(s) 463
SalI GTCGAC 1 cut(s) 1160
SatI GCNGC 1 cut(s) 450
Sau96I GGNCC 2 cut(s) 280, 619
SchI GAGTC 2 cut(s) 168, 189
ScrFI CCNGG 3 cut(s) 544, 796, 1202
SduI GDGCHC 1 cut(s) 987
SexAI ACCWGGT 1 cut(s) 542
SfaNI GCATC 1 cut(s) 505
SfcI CTRYAG 1 cut(s) 1029
Sfr303I CCGCGG 1 cut(s) 463
SfuI TTCGAA 2 cut(s) 363, 1230
SgrBI CCGCGG 1 cut(s) 463
SinI GGWCC 2 cut(s) 280, 619
Sse9I AATT 8 cut(s) 111, 247, 299, 332, 503, 820, 992, 1226
SsiI CCGC 4 cut(s) 460, 462, 1179, 1205
SspI AATATT 2 cut(s) 742, 835
SspMI CTAG 2 cut(s) 164, 341
StyD4I CCNGG 3 cut(s) 542, 794, 1200
StyI CCWWGG 4 cut(s) 27, 340, 531, 720
TaaI ACNGT 1 cut(s) 781
TaiI ACGT 4 cut(s) 9, 673, 1049, 1197
TaqI TCGA 6 cut(s) 70, 183, 363, 600, 1161, 1230
TasI AATT 8 cut(s) 111, 247, 299, 332, 503, 820, 992, 1226
TfiI GAWTC 2 cut(s) 602, 896
TseFI GTSAC 2 cut(s) 666, 902
TseI GCWGC 1 cut(s) 449
Tsp45I GTSAC 2 cut(s) 666, 902
TspDTI ATGAA 9 cut(s) 17, 345, 682, 813, 838, 900, 906, 985, 1013
Tth111I GACNNNGTC 1 cut(s) 682
Van91I CCANNNNNTGG 1 cut(s) 444
VpaK11BI GGWCC 2 cut(s) 280, 619
XapI RAATTY 6 cut(s) 299, 332, 503, 820, 992, 1226
XceI RCATGY 2 cut(s) 361, 1217
XmaJI CCTAGG 1 cut(s) 340
XmiI GTMKAC 2 cut(s) 198, 1161
XspI CTAG 2 cut(s) 164, 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.