Prupe.4G211100_v2.0.a1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
N/A
Physical Location & Seq
Forward (+)
13165502 .. 13168927
3426 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G211100.1_v2.0.a1.CDS.2

Sequence Viewer

Length: 1779 bp
ATGGATCCTTCTGTGTATGAGGCTGCAGAATCGGGTGATGTTGGTTTTTTCAGAAGAGCTGATGCATCAATTGATCTTCTCTGTCAGAAAACCCCAGCTCACAACAGTATTTTTCATATTGCAGCTGAGTACAAACGAATACATTTCTTCAGAGATGTCCTAGATGATCCATCTTTGCTGTTTTGGGCTCCCAATAAGAAGGGCAACACTCCTCTCCACGTCGCTGCGAGAGTAGGTTGTGATGAAATTGTAAAGCTGCTCATTGAACACGCAAAGAAACTTCTGCATATTGAAGGAGCTGACGAGGAAAGAGGACCGACTAATGGTGAAGCTAACAAACTACTTCGAATGACTAATTTTCAAGAAGATACAGCACTGCATGTTGCTGCCAGATGCGGTCACGTTGAGGTGGTGAATTTGTTAATGGCTGAAGATCCTGGATTGTGCTGTTTGATTAATTTTAATAAGGAGTCCCCCTTATTCTTGGCTACTCGGCATGGCTTTCCACTCGTAGCTCGTTCAATTTTGAACGAGTGTCCAATATCTCCTTCTTTTAGGGGGTTTAACGGTGTTACAACTTTGCATGTAGCGGTAACTTATATGGATGAAGAATGGAAAGGTATTGTGGAGACTATGGTGTCGAAATATCCGGATATAATAAAAAAGGCTGATGATGCACTTAGGTGGACTTCCTTACACTACGCAGCATTTGGAGGGAACCTTGAAGCAACTCAACTGTTGATGCAACGTGATAGTTCTGTTTCTTACATCTTGGACAAATCTGGAATGTCAGCTCTCCATGTCGCGGCCTATGCAGGTCACACCGATGTAATGGAAGAGATGATTCGGTGTCGGCCAGATATTTGTGATTTGGTCAATCATTATGGCCAAACGGCTCTACATGCTGCAGTTTTAGGTGGACGAACAAATGTCATCATGCATTCACTGAACGTGTCTAAGCTTGCAGTACTAATAAACGAAGCAGATGTAGATGGAAACACTCCGTTGCATCTGGCTGCCATCAAGAAAAATCCTGAAATTATAAGGGCGTTGACAGGCGACCCTAGAGTGGACAAGATTGCTACCAATGGGAAATTCTCACAGGCCTACAACATTTGTCTTAGCCAGAATATTGGAGAACAGGAAATCTTCGGCAGTAGTAGTCCTAGGGTTCTGGACAACCTAGGCAGCTCCATTGGTCTTCCATATTTCCATGGCCAAATCAGACGTGATTTCTGGAATGTTCCCGAGAAGGATACCATTGAAGGCGAGAAAGAACACGAGCAAAGACGTGGATCCAGACTCCTGATAGCAACGGTTGTTGCAGGCATTACATTTGCAGCAACTTTCGTCGATACTGGAGCATCAGGAAGCGATGGAAGAGGAATAGAGCTTGAAGTGTTTTATGTCTTGAACCTATTATCCCTCCTTTCCTCATTTTTTGTGATTTATAATGAAATAGTAGGCAAAAACTTGGTGATGCCAACGCGTTCAGCTTCAGAGTTCACTCGGCTTTCCGTCTCAGCAATGTTAGTAGCAAATATTGCGTGGTTGTCTGCAACGCAGCGCGGAGACACCGAATTGAGTCTGCTTGAAAGCGTACATTTTATAATCTCTCACATAATTGTTTTTCTTTGCTTCTTTTTTCTGCCGGAACTCACGGAAGCATTGAGGACGAAAGAAAAACAGAGACAATTATACAACAGAGGTTTTTCATTTTCTGCTTGGTATGTATTAGCTTATATAAGGAAGAAATTTCAGAAGCAACACGCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

593

Amino Acids

65.9

Weight (kDa)

6.2

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1043, 1452, 1610
AasI GACNNNNNNGTC 1 cut(s) 637
Acc36I ACCTGC 1 cut(s) 806
AccII CGCG 3 cut(s) 806, 1489, 1569
AccIII TCCGGA 1 cut(s) 649
AciI CCGC 4 cut(s) 396, 590, 806, 1569
AclWI GGATC 5 cut(s) 12, 161, 428, 1290, 1303
AcoI YGGCCR 3 cut(s) 854, 886, 1216
AcsI RAATTY 3 cut(s) 415, 1094, 1754
AcuI CTGAAG 3 cut(s) 133, 450, 1482
AfaI GTAC 3 cut(s) 131, 969, 1602
AfiI CCNNNNNNNGG 3 cut(s) 323, 805, 1069
AflIII ACRYGT 2 cut(s) 951, 1487
AjiI CACGTC 3 cut(s) 220, 1229, 1292
AjnI CCWGG 1 cut(s) 436
AjuI GAANNNNNNNTTGG 4 cut(s) 532, 564, 1079, 1111
AleI CACNNNNGTG 1 cut(s) 682
AloI GAACNNNNNNTCC 2 cut(s) 1406, 1438
Alw26I GTCTC 4 cut(s) 623, 1525, 1566, 1684
AlwI GGATC 5 cut(s) 12, 161, 428, 1290, 1303
Ama87I CYCGRG 1 cut(s) 1247
Aor13HI TCCGGA 1 cut(s) 649
AoxI GGCC 5 cut(s) 807, 854, 886, 1104, 1216
ApoI RAATTY 3 cut(s) 415, 1094, 1754
AseI ATTAAT 1 cut(s) 456
AspA2I CCTAGG 2 cut(s) 1166, 1183
AspLEI GCGC 1 cut(s) 1569
AspS9I GGNCC 1 cut(s) 314
AsuHPI GGTGA 4 cut(s) 47, 338, 424, 1489
AsuII TTCGAA 1 cut(s) 346
AvaI CYCGRG 1 cut(s) 1247
AvaII GGWCC 1 cut(s) 314
AvrII CCTAGG 2 cut(s) 1166, 1183
BalI TGGCCA 2 cut(s) 888, 1218
BamHI GGATCC 2 cut(s) 4, 1295
BanII GRGCYC 1 cut(s) 190
BauI CACGAG 1 cut(s) 1280
BbsI GAAGAC 1 cut(s) 1193
BccI CCATC 4 cut(s) 178, 986, 1028, 1370
BceAI ACGGC 1 cut(s) 909
BciT130I CCWGG 1 cut(s) 438
BciVI GTATCC 1 cut(s) 1249
BcoDI GTCTC 4 cut(s) 623, 1525, 1566, 1684
BfaI CTAG 4 cut(s) 161, 1065, 1167, 1184
BfmI CTRYAG 2 cut(s) 24, 906
BfuAI ACCTGC 1 cut(s) 806
BfuI GTATCC 1 cut(s) 1249
BlnI CCTAGG 2 cut(s) 1166, 1183
BmcAI AGTACT 1 cut(s) 969
Bme1390I CCNGG 1 cut(s) 438
Bme18I GGWCC 1 cut(s) 314
BmeT110I CYCGRG 1 cut(s) 1247
BmgBI CACGTC 3 cut(s) 220, 1229, 1292
BmgT120I GGNCC 1 cut(s) 314
BmiI GGNNCC 4 cut(s) 6, 189, 719, 1297
BmrFI CCNGG 1 cut(s) 438
BmsI GCATC 8 cut(s) 52, 74, 383, 664, 732, 1018, 1373, 1470
BpiI GAAGAC 1 cut(s) 1193
BpmI CTGGAG 1 cut(s) 1380
Bpu14I TTCGAA 1 cut(s) 346
BsaJI CCNNGG 3 cut(s) 1166, 1183, 1213
BsaWI WCCGGW 1 cut(s) 649
Bsc4I CCNNNNNNNGG 3 cut(s) 323, 805, 1069
Bse1I ACTGG 1 cut(s) 1363
Bse3DI GCAATG 1 cut(s) 1533
BseAI TCCGGA 1 cut(s) 649
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 3 cut(s) 1166, 1183, 1213
BseGI GGATG 1 cut(s) 610
BseLI CCNNNNNNNGG 3 cut(s) 323, 805, 1069
BseMI GCAATG 1 cut(s) 1533
BseMII CTCAG 2 cut(s) 117, 1536
BseNI ACTGG 1 cut(s) 1363
BseRI GAGGAG 1 cut(s) 201
BseYI CCCAGC 1 cut(s) 94
Bsh1236I CGCG 3 cut(s) 806, 1489, 1569
BshFI GGCC 5 cut(s) 809, 856, 888, 1106, 1218
BsiHKCI CYCGRG 1 cut(s) 1247
BsiSI CCGG 2 cut(s) 650, 1652
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 3 cut(s) 323, 805, 1069
BsmAI GTCTC 4 cut(s) 623, 1525, 1566, 1684
BsmBI CGTCTC 1 cut(s) 1525
BsmFI GGGAC 1 cut(s) 457
BsmI GAATGC 1 cut(s) 940
BsnI GGCC 5 cut(s) 809, 856, 888, 1106, 1218
BsoBI CYCGRG 1 cut(s) 1247
Bsp119I TTCGAA 1 cut(s) 346
Bsp1286I GDGCHC 1 cut(s) 190
Bsp13I TCCGGA 1 cut(s) 649
Bsp143I GATC 5 cut(s) 4, 73, 166, 433, 1295
Bsp19I CCATGG 1 cut(s) 1213
BspACI CCGC 4 cut(s) 396, 590, 806, 1569
BspANI GGCC 5 cut(s) 809, 856, 888, 1106, 1218
BspCNI CTCAG 2 cut(s) 118, 1535
BspEI TCCGGA 1 cut(s) 649
BspFNI CGCG 3 cut(s) 806, 1489, 1569
BspLI GGNNCC 4 cut(s) 6, 189, 719, 1297
BspMAI CTGCAG 2 cut(s) 28, 910
BspMI ACCTGC 1 cut(s) 806
BspPI GGATC 5 cut(s) 12, 161, 428, 1290, 1303
BspQI GCTCTTC 1 cut(s) 49
BspT104I TTCGAA 1 cut(s) 346
BsrDI GCAATG 1 cut(s) 1533
BsrI ACTGG 1 cut(s) 1363
BssECI CCNNGG 3 cut(s) 1166, 1183, 1213
BssMI GATC 5 cut(s) 4, 73, 166, 433, 1295
BssSI CACGAG 1 cut(s) 1280
BssT1I CCWWGG 3 cut(s) 1166, 1183, 1213
Bst2BI CACGAG 1 cut(s) 1280
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 4 cut(s) 107, 569, 738, 1318
Bst6I CTCTTC 3 cut(s) 49, 831, 1375
BstAPI GCANNNNNTGC 1 cut(s) 1544
BstBI TTCGAA 1 cut(s) 346
BstC8I GCNNGC 2 cut(s) 963, 1327
BstDEI CTNAG 5 cut(s) 126, 680, 957, 1121, 1522
BstDSI CCRYGG 1 cut(s) 1213
BstF5I GGATG 1 cut(s) 610
BstFNI CGCG 3 cut(s) 806, 1489, 1569
BstHHI GCGC 1 cut(s) 1569
BstKTI GATC 5 cut(s) 7, 76, 169, 436, 1298
BstMAI GTCTC 4 cut(s) 623, 1525, 1566, 1684
BstMBI GATC 5 cut(s) 4, 73, 166, 433, 1295
BstMWI GCNNNNNNNGC 4 cut(s) 674, 812, 902, 1544
BstNI CCWGG 1 cut(s) 438
BstNSI RCATGY 3 cut(s) 383, 587, 905
BstSCI CCNGG 1 cut(s) 436
BstSFI CTRYAG 2 cut(s) 24, 906
BstUI CGCG 3 cut(s) 806, 1489, 1569
BstV2I GAAGAC 1 cut(s) 1193
BstX2I RGATCY 3 cut(s) 4, 433, 1295
BstXI CCANNNNNNTGG 1 cut(s) 1133
BstYI RGATCY 3 cut(s) 4, 433, 1295
BsuI GTATCC 1 cut(s) 1249
BsuRI GGCC 5 cut(s) 809, 856, 888, 1106, 1218
BtgI CCRYGG 1 cut(s) 1213
BtgZI GCGATG 1 cut(s) 1389
BtrI CACGTC 3 cut(s) 220, 1229, 1292
BtsCI GGATG 1 cut(s) 610
BtsI GCAGTG 1 cut(s) 374
BtsIMutI CAGTG 2 cut(s) 374, 944
BveI ACCTGC 1 cut(s) 806
Cac8I GCNNGC 2 cut(s) 963, 1327
CfoI GCGC 1 cut(s) 1569
Cfr13I GGNCC 1 cut(s) 314
Csp6I GTAC 3 cut(s) 130, 968, 1601
CviAII CATG 7 cut(s) 380, 497, 584, 800, 902, 937, 1214
CviQI GTAC 3 cut(s) 130, 968, 1601
DdeI CTNAG 5 cut(s) 126, 680, 957, 1121, 1522
DpnI GATC 5 cut(s) 6, 75, 168, 435, 1297
DpnII GATC 5 cut(s) 4, 73, 166, 433, 1295
DrdI GACNNNNNNGTC 1 cut(s) 637
DseDI GACNNNNNNGTC 1 cut(s) 637
EaeI YGGCCR 3 cut(s) 854, 886, 1216
Eam1104I CTCTTC 3 cut(s) 49, 831, 1375
EarI CTCTTC 3 cut(s) 49, 831, 1375
Eco130I CCWWGG 3 cut(s) 1166, 1183, 1213
Eco147I AGGCCT 1 cut(s) 1106
Eco24I GRGCYC 1 cut(s) 190
Eco47I GGWCC 1 cut(s) 314
Eco57I CTGAAG 3 cut(s) 133, 450, 1482
Eco88I CYCGRG 1 cut(s) 1247
EcoRII CCWGG 1 cut(s) 436
EcoT14I CCWWGG 3 cut(s) 1166, 1183, 1213
EcoT22I ATGCAT 2 cut(s) 67, 942
EcoT38I GRGCYC 1 cut(s) 190
ErhI CCWWGG 3 cut(s) 1166, 1183, 1213
Esp3I CGTCTC 1 cut(s) 1525
FaeI CATG 7 cut(s) 383, 500, 587, 803, 905, 940, 1217
FaqI GGGAC 1 cut(s) 457
FatI CATG 7 cut(s) 379, 496, 583, 799, 901, 936, 1213
FokI GGATG 1 cut(s) 617
FriOI GRGCYC 1 cut(s) 190
FspBI CTAG 4 cut(s) 161, 1065, 1167, 1184
GlaI GCGC 1 cut(s) 1568
GsaI CCCAGC 1 cut(s) 98
GsuI CTGGAG 1 cut(s) 1380
HaeIII GGCC 5 cut(s) 809, 856, 888, 1106, 1218
HapII CCGG 2 cut(s) 650, 1652
HhaI GCGC 1 cut(s) 1569
Hin1II CATG 7 cut(s) 383, 500, 587, 803, 905, 940, 1217
Hin6I GCGC 1 cut(s) 1567
HinP1I GCGC 1 cut(s) 1567
HincII GTYRAC 1 cut(s) 1053
HindII GTYRAC 1 cut(s) 1053
HindIII AAGCTT 1 cut(s) 959
HinfI GANTC 5 cut(s) 29, 470, 844, 1302, 1585
HpaII CCGG 2 cut(s) 650, 1652
HphI GGTGA 4 cut(s) 47, 338, 424, 1489
Hpy166II GTNNAC 5 cut(s) 687, 920, 1053, 1072, 1506
Hpy188I TCNGA 6 cut(s) 53, 87, 152, 1226, 1501, 1761
Hpy8I GTNNAC 5 cut(s) 687, 920, 1053, 1072, 1506
Hpy99I CGWCG 2 cut(s) 224, 1355
HpyAV CCTTC 6 cut(s) 18, 193, 287, 558, 1246, 1259
HpyCH4III ACNGT 4 cut(s) 107, 569, 738, 1318
HpyCH4IV ACGT 6 cut(s) 219, 402, 748, 951, 1228, 1291
HpyF10VI GCNNNNNNNGC 4 cut(s) 674, 812, 902, 1544
HpyF3I CTNAG 5 cut(s) 126, 680, 957, 1121, 1522
HpySE526I ACGT 6 cut(s) 219, 402, 748, 951, 1228, 1291
Hsp92II CATG 7 cut(s) 383, 500, 587, 803, 905, 940, 1217
HspAI GCGC 1 cut(s) 1567
Kpn2I TCCGGA 1 cut(s) 649
Kzo9I GATC 5 cut(s) 4, 73, 166, 433, 1295
LguI GCTCTTC 1 cut(s) 49
LmnI GCTCC 4 cut(s) 193, 296, 1196, 1361
LweI GCATC 8 cut(s) 52, 74, 383, 664, 732, 1018, 1373, 1470
MaeI CTAG 4 cut(s) 161, 1065, 1167, 1184
MaeII ACGT 6 cut(s) 219, 402, 748, 951, 1228, 1291
MaeIII GTNAC 4 cut(s) 398, 571, 592, 818
MalI GATC 5 cut(s) 6, 75, 168, 435, 1297
MboI GATC 5 cut(s) 4, 73, 166, 433, 1295
MfeI CAATTG 1 cut(s) 69
MflI RGATCY 3 cut(s) 4, 433, 1295
MhlI GDGCHC 1 cut(s) 190
MlsI TGGCCA 2 cut(s) 888, 1218
MluI ACGCGT 1 cut(s) 1487
MluNI TGGCCA 2 cut(s) 888, 1218
MlyI GAGTC 3 cut(s) 479, 1296, 1594
Mox20I TGGCCA 2 cut(s) 888, 1218
Mph1103I ATGCAT 2 cut(s) 67, 942
MroI TCCGGA 1 cut(s) 649
MscI TGGCCA 2 cut(s) 888, 1218
MseI TTAA 4 cut(s) 422, 456, 462, 564
MslI CAYNNNNRTG 2 cut(s) 682, 825
Msp20I TGGCCA 2 cut(s) 888, 1218
MspA1I CMGCKG 1 cut(s) 125
MspI CCGG 2 cut(s) 650, 1652
MspR9I CCNGG 1 cut(s) 438
MunI CAATTG 1 cut(s) 69
Mva1269I GAATGC 1 cut(s) 940
MvaI CCWGG 1 cut(s) 438
MvnI CGCG 3 cut(s) 806, 1489, 1569
MwoI GCNNNNNNNGC 4 cut(s) 674, 812, 902, 1544
NcoI CCATGG 1 cut(s) 1213
NdeII GATC 5 cut(s) 4, 73, 166, 433, 1295
NlaIII CATG 7 cut(s) 383, 500, 587, 803, 905, 940, 1217
NlaIV GGNNCC 4 cut(s) 6, 189, 719, 1297
NmeAIII GCCGAG 2 cut(s) 472, 1489
NmuCI GTSAC 2 cut(s) 398, 818
NsiI ATGCAT 2 cut(s) 67, 942
NspI RCATGY 3 cut(s) 383, 587, 905
NspV TTCGAA 1 cut(s) 346
OliI CACNNNNGTG 1 cut(s) 682
PceI AGGCCT 1 cut(s) 1106
PciSI GCTCTTC 1 cut(s) 49
PctI GAATGC 1 cut(s) 940
PfeI GAWTC 2 cut(s) 29, 844
PfoI TCCNGGA 1 cut(s) 436
PleI GAGTC 3 cut(s) 478, 1296, 1593
PpsI GAGTC 3 cut(s) 478, 1296, 1593
PshBI ATTAAT 1 cut(s) 456
PsiI TTATAA 3 cut(s) 1043, 1452, 1610
Psp6I CCWGG 1 cut(s) 436
PspFI CCCAGC 1 cut(s) 94
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 4 cut(s) 6, 189, 719, 1297
PspPI GGNCC 1 cut(s) 314
PstI CTGCAG 2 cut(s) 28, 910
PsuI RGATCY 3 cut(s) 4, 433, 1295
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 3 cut(s) 131, 969, 1602
RsaNI GTAC 3 cut(s) 130, 968, 1601
RseI CAYNNNNRTG 2 cut(s) 682, 825
SapI GCTCTTC 1 cut(s) 49
SaqAI TTAA 4 cut(s) 422, 456, 462, 564
Sau3AI GATC 5 cut(s) 4, 73, 166, 433, 1295
Sau96I GGNCC 1 cut(s) 314
ScaI AGTACT 1 cut(s) 969
SchI GAGTC 3 cut(s) 479, 1296, 1594
ScrFI CCNGG 1 cut(s) 438
SduI GDGCHC 1 cut(s) 190
SfaNI GCATC 8 cut(s) 52, 74, 383, 664, 732, 1018, 1373, 1470
SfcI CTRYAG 2 cut(s) 24, 906
SfuI TTCGAA 1 cut(s) 346
SinI GGWCC 1 cut(s) 314
SmiMI CAYNNNNRTG 2 cut(s) 682, 825
SseBI AGGCCT 1 cut(s) 1106
SsiI CCGC 4 cut(s) 396, 590, 806, 1569
SspI AATATT 2 cut(s) 1132, 1543
SspMI CTAG 4 cut(s) 161, 1065, 1167, 1184
StuI AGGCCT 1 cut(s) 1106
StyD4I CCNGG 1 cut(s) 436
StyI CCWWGG 3 cut(s) 1166, 1183, 1213
TaaI ACNGT 4 cut(s) 107, 569, 738, 1318
TaiI ACGT 6 cut(s) 222, 405, 751, 954, 1231, 1294
TaqI TCGA 3 cut(s) 346, 641, 1353
TaqII GACCGA 1 cut(s) 331
TatI WGTACW 2 cut(s) 129, 967
TauI GCSGC 1 cut(s) 809
TfiI GAWTC 2 cut(s) 29, 844
Tru1I TTAA 4 cut(s) 422, 456, 462, 564
Tru9I TTAA 4 cut(s) 422, 456, 462, 564
TscAI CASTG 2 cut(s) 381, 951
TseFI GTSAC 2 cut(s) 398, 818
Tsp45I GTSAC 2 cut(s) 398, 818
TspDTI ATGAA 5 cut(s) 104, 258, 621, 1470, 1704
TspGWI ACGGA 3 cut(s) 993, 1507, 1676
TspRI CASTG 2 cut(s) 381, 951
VpaK11BI GGWCC 1 cut(s) 314
VspI ATTAAT 1 cut(s) 456
XapI RAATTY 3 cut(s) 415, 1094, 1754
XceI RCATGY 3 cut(s) 383, 587, 905
XmaJI CCTAGG 2 cut(s) 1166, 1183
XspI CTAG 4 cut(s) 161, 1065, 1167, 1184
ZrmI AGTACT 1 cut(s) 969
Zsp2I ATGCAT 2 cut(s) 67, 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.