Prupe.4G216400_v2.0.a1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
N/A
Physical Location & Seq
Forward (+)
13563491 .. 13565423
1933 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G216400.1_v2.0.a1.CDS.6

Sequence Viewer

Length: 735 bp
ATGTTTGACAACTCCTTAGTCGATGTGGGTAATAATAATTACCTAAAGGCTAATTTCCCTCACAATGGTGTTGATCTTCCCAACAAGAAAGCAACGGGAAGGTTTGGGAATGGCAAGAATGCTGCGGATTTTCTTGTAAACTACTATTCGCAAGTGTATGAAAACATCAGGCTAGAACTGGGCTCCAACGATTCCATGGTCTCAAATCTAAAACAACAATTTGAGAGAATACATTCTTTTGGCGCTCGAAAATTTGTAGTTGTTGAGACTCGGCTAGTTGGATTCTGCCCAGCTCAAAGGGCTTCAAATGAGCGACAGTGCAACGAAGATGCAAACTCCATGTCTGTCCACTACAATGACAGCCTTAAGTCGATGTTACAGCAATTGAAAGCGGGATTGCACGTAAATTACACCTTCTTCAAGACTTACAGTGTCATCACTGACCATTCAAAACGCAGCTTCTTCCGGTACTCATCTTATTTTCCTACACTACAAGTCTTTCCTGCATGTTATGTTATTAGACTGAAAAGTAAAGGATTTACAGAGACCGAAGCTGCATGCTGTGGTATTGGGAAGCTTAACGCCCTTGCTGCTTGCCTGCCGGTTTCGTCTTTGTGCTCCAACAGAAGGGACTATGTCTTCTGGGACCGATTCCATCCTACAGAGGCAACTCATGGTAGCTTGGTGGACACTTTTCGGTGGTCCTTTCAGAATACACATTCCCAACGAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

245

Amino Acids

27.85

Weight (kDa)

9.14

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 125, 392
AcsI RAATTY 1 cut(s) 251
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 2 cut(s) 65, 627
AflII CTTAAG 1 cut(s) 365
AgsI TTSAA 4 cut(s) 306, 388, 421, 450
AleI CACNNNNGTG 1 cut(s) 66
AluBI AGCT 5 cut(s) 293, 459, 554, 577, 681
AluI AGCT 5 cut(s) 293, 459, 554, 577, 681
Alw21I GWGCWC 1 cut(s) 620
Alw26I GTCTC 3 cut(s) 205, 260, 539
ApeKI GCWGC 4 cut(s) 122, 456, 554, 590
ApoI RAATTY 1 cut(s) 251
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
Asp700I GAANNNNTTC 1 cut(s) 232
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 2 cut(s) 646, 702
AvaII GGWCC 2 cut(s) 646, 702
BanII GRGCYC 1 cut(s) 185
BbsI GAAGAC 1 cut(s) 631
Bbv12I GWGCWC 1 cut(s) 620
BbvI GCAGC 4 cut(s) 109, 468, 541, 577
BccI CCATC 1 cut(s) 663
BcoDI GTCTC 3 cut(s) 205, 260, 539
BfaI CTAG 2 cut(s) 173, 275
BfmI CTRYAG 1 cut(s) 660
BfoI RGCGCY 1 cut(s) 246
BfrI CTTAAG 1 cut(s) 365
BisI GCNGC 4 cut(s) 123, 457, 555, 591
BlsI GCNGC 4 cut(s) 124, 458, 556, 592
Bme18I GGWCC 2 cut(s) 646, 702
BmgT120I GGNCC 2 cut(s) 646, 702
BmiI GGNNCC 2 cut(s) 184, 647
BmrI ACTGGG 1 cut(s) 188
BmsI GCATC 1 cut(s) 319
BmuI ACTGGG 1 cut(s) 188
BpiI GAAGAC 1 cut(s) 631
BsaAI YACGTR 1 cut(s) 403
BsaI GGTCTC 2 cut(s) 205, 539
BsaJI CCNNGG 1 cut(s) 195
BsaWI WCCGGW 1 cut(s) 465
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 627
Bse118I RCCGGY 1 cut(s) 601
Bse1I ACTGG 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 195
BseGI GGATG 1 cut(s) 655
BseLI CCNNNNNNNGG 2 cut(s) 65, 627
BseNI ACTGG 1 cut(s) 183
BseXI GCAGC 4 cut(s) 109, 468, 541, 577
BseYI CCCAGC 1 cut(s) 289
BsiHKAI GWGCWC 1 cut(s) 620
BsiSI CCGG 2 cut(s) 466, 602
BslFI GGGAC 2 cut(s) 644, 659
BslI CCNNNNNNNGG 2 cut(s) 65, 627
BsmAI GTCTC 3 cut(s) 205, 260, 539
BsmFI GGGAC 2 cut(s) 644, 659
BsmI GAATGC 1 cut(s) 124
Bso31I GGTCTC 2 cut(s) 205, 539
Bsp1286I GDGCHC 2 cut(s) 185, 620
Bsp143I GATC 1 cut(s) 73
Bsp19I CCATGG 1 cut(s) 195
BspACI CCGC 2 cut(s) 125, 392
BspLI GGNNCC 2 cut(s) 184, 647
BspTI CTTAAG 1 cut(s) 365
BspTNI GGTCTC 2 cut(s) 205, 539
BsrFI RCCGGY 1 cut(s) 601
BsrI ACTGG 1 cut(s) 183
BssAI RCCGGY 1 cut(s) 601
BssECI CCNNGG 1 cut(s) 195
BssMI GATC 1 cut(s) 73
BssT1I CCWWGG 1 cut(s) 195
Bst4CI ACNGT 2 cut(s) 318, 431
BstAFI CTTAAG 1 cut(s) 365
BstBAI YACGTR 1 cut(s) 403
BstC8I GCNNGC 3 cut(s) 559, 595, 599
BstDEI CTNAG 1 cut(s) 16
BstDSI CCRYGG 1 cut(s) 195
BstF5I GGATG 1 cut(s) 655
BstH2I RGCGCY 1 cut(s) 246
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 1 cut(s) 76
BstMAI GTCTC 3 cut(s) 205, 260, 539
BstMBI GATC 1 cut(s) 73
BstMWI GCNNNNNNNGC 2 cut(s) 299, 590
BstNSI RCATGY 2 cut(s) 510, 561
BstSFI CTRYAG 1 cut(s) 660
BstV1I GCAGC 4 cut(s) 109, 468, 541, 577
BstV2I GAAGAC 1 cut(s) 631
BtgI CCRYGG 1 cut(s) 195
BtsCI GGATG 1 cut(s) 655
BtsIMutI CAGTG 3 cut(s) 323, 436, 438
Cac8I GCNNGC 3 cut(s) 559, 595, 599
CfoI GCGC 1 cut(s) 245
Cfr10I RCCGGY 1 cut(s) 601
Cfr13I GGNCC 2 cut(s) 646, 702
Csp6I GTAC 1 cut(s) 469
CviAII CATG 5 cut(s) 196, 340, 507, 558, 674
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 1 cut(s) 16
DpnI GATC 1 cut(s) 75
DpnII GATC 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 195
Eco24I GRGCYC 1 cut(s) 185
Eco31I GGTCTC 2 cut(s) 205, 539
Eco47I GGWCC 2 cut(s) 646, 702
EcoT14I CCWWGG 1 cut(s) 195
EcoT38I GRGCYC 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 5 cut(s) 199, 343, 510, 561, 677
FaiI YATR 8 cut(s) 159, 197, 341, 508, 513, 559, 636, 675
FaqI GGGAC 2 cut(s) 644, 659
FatI CATG 5 cut(s) 195, 339, 506, 557, 673
FauI CCCGC 1 cut(s) 385
Fnu4HI GCNGC 4 cut(s) 123, 457, 555, 591
FokI GGATG 1 cut(s) 642
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 4 cut(s) 123, 457, 555, 591
FspBI CTAG 2 cut(s) 173, 275
GlaI GCGC 1 cut(s) 244
GluI GCNGC 4 cut(s) 123, 457, 555, 591
GsaI CCCAGC 1 cut(s) 293
HaeII RGCGCY 1 cut(s) 246
HapII CCGG 2 cut(s) 466, 602
HhaI GCGC 1 cut(s) 245
Hin1II CATG 5 cut(s) 199, 343, 510, 561, 677
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HindIII AAGCTT 1 cut(s) 575
HinfI GANTC 4 cut(s) 191, 268, 282, 651
HpaII CCGG 2 cut(s) 466, 602
Hpy166II GTNNAC 3 cut(s) 139, 349, 688
Hpy188I TCNGA 1 cut(s) 711
Hpy188III TCNNGA 1 cut(s) 421
Hpy8I GTNNAC 3 cut(s) 139, 349, 688
HpyAV CCTTC 3 cut(s) 93, 424, 621
HpyCH4III ACNGT 2 cut(s) 318, 431
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 5 cut(s) 321, 332, 400, 506, 557
HpyF10VI GCNNNNNNNGC 2 cut(s) 299, 590
HpyF3I CTNAG 1 cut(s) 16
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 5 cut(s) 199, 343, 510, 561, 677
HspAI GCGC 1 cut(s) 243
Kzo9I GATC 1 cut(s) 73
LmnI GCTCC 2 cut(s) 188, 623
LpnPI CCDG 8 cut(s) 154, 164, 303, 479, 516, 611, 615, 628
Lsp1109I GCAGC 4 cut(s) 109, 468, 541, 577
LweI GCATC 1 cut(s) 319
MaeI CTAG 2 cut(s) 173, 275
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 1 cut(s) 375
MalI GATC 1 cut(s) 75
MboI GATC 1 cut(s) 73
MboII GAAGA 5 cut(s) 68, 338, 409, 454, 631
MfeI CAATTG 1 cut(s) 383
MhlI GDGCHC 2 cut(s) 185, 620
MluCI AATT 6 cut(s) 37, 52, 218, 251, 383, 406
MlyI GAGTC 1 cut(s) 262
MmeI TCCRAC 3 cut(s) 210, 259, 645
MnlI CCTC 2 cut(s) 69, 658
MroXI GAANNNNTTC 1 cut(s) 232
MseI TTAA 2 cut(s) 366, 579
MslI CAYNNNNRTG 2 cut(s) 66, 354
MspCI CTTAAG 1 cut(s) 365
MspI CCGG 2 cut(s) 466, 602
MunI CAATTG 1 cut(s) 383
Mva1269I GAATGC 1 cut(s) 124
MwoI GCNNNNNNNGC 2 cut(s) 299, 590
NcoI CCATGG 1 cut(s) 195
NdeII GATC 1 cut(s) 73
NlaIII CATG 5 cut(s) 199, 343, 510, 561, 677
NlaIV GGNNCC 2 cut(s) 184, 647
NmeAIII GCCGAG 1 cut(s) 250
NspI RCATGY 2 cut(s) 510, 561
OliI CACNNNNGTG 1 cut(s) 66
PaeI GCATGC 1 cut(s) 561
PctI GAATGC 1 cut(s) 124
PdmI GAANNNNTTC 1 cut(s) 232
PfeI GAWTC 3 cut(s) 191, 282, 651
PflFI GACNNNGTC 1 cut(s) 635
PkrI GCNGC 4 cut(s) 124, 458, 556, 592
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
Ppu21I YACGTR 1 cut(s) 403
PspFI CCCAGC 1 cut(s) 289
PspN4I GGNNCC 2 cut(s) 184, 647
PspPI GGNCC 2 cut(s) 646, 702
PsyI GACNNNGTC 1 cut(s) 635
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
RseI CAYNNNNRTG 2 cut(s) 66, 354
SaqAI TTAA 2 cut(s) 366, 579
SatI GCNGC 4 cut(s) 123, 457, 555, 591
Sau3AI GATC 1 cut(s) 73
Sau96I GGNCC 2 cut(s) 646, 702
SchI GAGTC 1 cut(s) 262
SduI GDGCHC 2 cut(s) 185, 620
SetI ASST 9 cut(s) 45, 104, 295, 405, 416, 461, 556, 579, 683
SfaNI GCATC 1 cut(s) 319
SfcI CTRYAG 1 cut(s) 660
SinI GGWCC 2 cut(s) 646, 702
SmiMI CAYNNNNRTG 2 cut(s) 66, 354
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
SphI GCATGC 1 cut(s) 561
Sse9I AATT 6 cut(s) 37, 52, 218, 251, 383, 406
SsiI CCGC 2 cut(s) 125, 392
SspMI CTAG 2 cut(s) 173, 275
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 2 cut(s) 318, 431
TaiI ACGT 1 cut(s) 405
TaqI TCGA 3 cut(s) 21, 247, 371
TaqII GACCGA 2 cut(s) 563, 663
TasI AATT 6 cut(s) 37, 52, 218, 251, 383, 406
TfiI GAWTC 3 cut(s) 191, 282, 651
Tru1I TTAA 2 cut(s) 366, 579
Tru9I TTAA 2 cut(s) 366, 579
TscAI CASTG 3 cut(s) 323, 436, 445
TseI GCWGC 4 cut(s) 122, 456, 554, 590
TspDTI ATGAA 1 cut(s) 174
TspRI CASTG 3 cut(s) 323, 436, 445
Tth111I GACNNNGTC 1 cut(s) 635
Vha464I CTTAAG 1 cut(s) 365
VpaK11BI GGWCC 2 cut(s) 646, 702
XapI RAATTY 1 cut(s) 251
XceI RCATGY 2 cut(s) 510, 561
XcmI CCANNNNNNNNNTGG 1 cut(s) 193
XmnI GAANNNNTTC 1 cut(s) 232
XspI CTAG 2 cut(s) 173, 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.