Prupe.4G275400_v2.0.a1

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
N/A
Physical Location & Seq
Reverse (-)
22957097 .. 22958555
1459 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G275400.1_v2.0.a1.CDS.3

Sequence Viewer

Length: 996 bp
ATGGGCCTGTTTTTTGAACTTTGGCGTGCCAAATTTAGGCCCAAACACCTTGCTGCAGCAACATTGAGACCTGAGACCATGTATGGACAAACAAATGCATGGGTATTGCCCAATGGAAGGTATGGAGCCTTTGAAGTCAATGAAACAGATGTATTTATTCTTACGCAAAGAGCAGCACGTAATCTTGCTTATCAGGGGTTCTTTAGGATACCTGAGAAACCTTCCTGCTTCATTGAACTAACTGATTGTACCGATCATCAATTTGGAGATAAGGCTGCGGAGAAGGTCTGTGGGGATCTCAAGATCAAAAGCCAGAATGAGAAAGAGAAGCTAGCAGAAGCCAAGAGAGAATCGGAGAAGCACGTAGTGTCACGGTCAGGTGATGAATGTGTTGTGGCTCAAACAGAGCAATGGTACATCTTTTATGGTGAAGCTGAATGGAAAACTTTGGCTGAGGAATGCTTGTCGAGCATGAACTTGTACTCTGACGTGACACAACATGGATTTGAACACACGTTAAGTTGGTTGAATCAGTGGGCTTGTTCACGATCGTTTGGGCTTGGAACACGTATTCCCTGGGATGAAAAATACTTGGTTGAGTCGTTGTCTGATTCAACCCTTTACATGGCTTATTACACCATTGCTCACTTTTTACACGATGGGGACATGTATGGGGATTCCACCACATCTGGGGTTATACCTGAACAAATGACCGATGAGTGTTTGGTAAGGATCTCATCTAGAATCATTTTAACATTTTGCATTTACAACCACACGACAATTATGCTCAAGAAGCATTGGCCTCGTGGGTATAGATGTAATGGGCACCTCATGCTCAATTCTAAGAAGATGTCCAAGTCCACAGGGAATTTTTTGACATTACGGCTGGCAATAGAGGAATATTCTGCTGATGCCACACGAATGGCTGCCGATGCCGGTGATGGTGTTGATGATGCAAATTTTGTATTTGACACTGCAAATGCTGCCATTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000323 GO:0001101 GO:0003674 GO:0003824 GO:0004812 GO:0004819 GO:0004823 GO:0004832 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006425 GO:0006429 GO:0006438 GO:0006518 GO:0006520 GO:0006605 GO:0006622 GO:0006623 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0007034 GO:0007041 GO:0007154 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009267 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009719 GO:0009892 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010506 GO:0010507 GO:0010646 GO:0010647 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016070 GO:0016604 GO:0016874 GO:0016875 GO:0017101 GO:0019222 GO:0019538 GO:0019752 GO:0023051 GO:0023056 GO:0030054 GO:0030234 GO:0030695 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032006 GO:0032008 GO:0032535 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034198 GO:0034613 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0042594 GO:0042886 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043087 GO:0043170 GO:0043200 GO:0043201 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043547 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0051716 GO:0055044 GO:0060589 GO:0061462 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071233 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090066 GO:0090304 GO:0098772 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903432 GO:1904263 GO:1990253 GO:1990928
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
Anticodon_1 tRNA-synt_1

Protein Analysis

332

Amino Acids

37.68

Weight (kDa)

5.62

Isoelectric Point (pI)

36.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 825
AciI CCGC 1 cut(s) 278
AclWI GGATC 2 cut(s) 303, 740
AcsI RAATTY 3 cut(s) 32, 868, 958
AdeI CACNNNGTG 1 cut(s) 367
AfaI GTAC 3 cut(s) 250, 416, 482
AfiI CCNNNNNNNGG 4 cut(s) 36, 117, 625, 690
AflIII ACRYGT 3 cut(s) 513, 566, 666
AgsI TTSAA 6 cut(s) 17, 134, 236, 509, 529, 615
AjiI CACGTC 1 cut(s) 490
AjnI CCWGG 1 cut(s) 575
AloI GAACNNNNNNTCC 2 cut(s) 556, 588
AluBI AGCT 2 cut(s) 331, 434
AluI AGCT 2 cut(s) 331, 434
Alw26I GTCTC 2 cut(s) 61, 68
AlwI GGATC 2 cut(s) 303, 740
AoxI GGCC 3 cut(s) 4, 38, 800
ApeKI GCWGC 6 cut(s) 53, 56, 173, 275, 926, 983
ApoI RAATTY 3 cut(s) 32, 868, 958
AspS9I GGNCC 2 cut(s) 4, 39
AsuHPI GGTGA 3 cut(s) 392, 440, 950
AsuNHI GCTAGC 1 cut(s) 331
BaeGI GKGCMC 1 cut(s) 828
BanI GGYRCC 1 cut(s) 825
BauI CACGAG 1 cut(s) 804
BbvCI CCTCAGC 1 cut(s) 453
BbvI GCAGC 6 cut(s) 40, 68, 185, 262, 913, 970
BccI CCATC 2 cut(s) 653, 935
BceAI ACGGC 1 cut(s) 899
BcgI CGANNNNNNTGC 4 cut(s) 766, 785, 800, 819
BciT130I CCWGG 1 cut(s) 577
BciVI GTATCC 1 cut(s) 201
BcoDI GTCTC 2 cut(s) 61, 68
BfaI CTAG 2 cut(s) 332, 741
BfmI CTRYAG 1 cut(s) 54
BfuI GTATCC 1 cut(s) 201
BisI GCNGC 6 cut(s) 54, 57, 174, 276, 927, 984
BlsI GCNGC 6 cut(s) 55, 58, 175, 277, 928, 985
Bme1390I CCNGG 1 cut(s) 577
BmgBI CACGTC 1 cut(s) 490
BmgT120I GGNCC 2 cut(s) 4, 39
BmiI GGNNCC 2 cut(s) 127, 827
BmrFI CCNGG 1 cut(s) 577
BmsI GCATC 3 cut(s) 901, 922, 943
BmtI GCTAGC 1 cut(s) 335
Bpu10I CCTNAGC 1 cut(s) 453
BpuEI CTTGAG 2 cut(s) 284, 773
BsaAI YACGTR 3 cut(s) 179, 364, 569
BsaI GGTCTC 2 cut(s) 61, 68
BsaJI CCNNGG 2 cut(s) 575, 576
Bsc4I CCNNNNNNNGG 4 cut(s) 36, 117, 625, 690
Bse118I RCCGGY 1 cut(s) 935
Bse3DI GCAATG 2 cut(s) 416, 639
BseBI CCWGG 1 cut(s) 577
BseDI CCNNGG 2 cut(s) 575, 576
BseGI GGATG 1 cut(s) 586
BseLI CCNNNNNNNGG 4 cut(s) 36, 117, 625, 690
BseMI GCAATG 2 cut(s) 416, 639
BseMII CTCAG 3 cut(s) 63, 204, 444
BseSI GKGCMC 1 cut(s) 828
BseXI GCAGC 6 cut(s) 40, 68, 185, 262, 913, 970
Bsh1285I CGRYCG 1 cut(s) 551
BshFI GGCC 3 cut(s) 6, 40, 802
BshNI GGYRCC 1 cut(s) 825
BsiEI CGRYCG 1 cut(s) 551
BsiSI CCGG 1 cut(s) 936
BslFI GGGAC 1 cut(s) 677
BslI CCNNNNNNNGG 4 cut(s) 36, 117, 625, 690
BsmAI GTCTC 2 cut(s) 61, 68
BsmFI GGGAC 1 cut(s) 677
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 3 cut(s) 6, 40, 802
Bso31I GGTCTC 2 cut(s) 61, 68
Bsp1286I GDGCHC 1 cut(s) 828
Bsp143I GATC 5 cut(s) 253, 295, 303, 548, 732
BspACI CCGC 1 cut(s) 278
BspANI GGCC 3 cut(s) 6, 40, 802
BspCNI CTCAG 3 cut(s) 64, 205, 445
BspLI GGNNCC 2 cut(s) 127, 827
BspMAI CTGCAG 1 cut(s) 58
BspOI GCTAGC 1 cut(s) 335
BspPI GGATC 2 cut(s) 303, 740
BspT107I GGYRCC 1 cut(s) 825
BspTNI GGTCTC 2 cut(s) 61, 68
BsrDI GCAATG 2 cut(s) 416, 639
BsrFI RCCGGY 1 cut(s) 935
BssAI RCCGGY 1 cut(s) 935
BssECI CCNNGG 2 cut(s) 575, 576
BssMI GATC 5 cut(s) 253, 295, 303, 548, 732
BssSI CACGAG 1 cut(s) 804
Bst2BI CACGAG 1 cut(s) 804
Bst2UI CCWGG 1 cut(s) 577
Bst4CI ACNGT 1 cut(s) 375
BstAPI GCANNNNNTGC 2 cut(s) 832, 983
BstBAI YACGTR 3 cut(s) 179, 364, 569
BstC8I GCNNGC 3 cut(s) 27, 333, 888
BstDEI CTNAG 4 cut(s) 72, 213, 453, 843
BstF5I GGATG 1 cut(s) 586
BstKTI GATC 5 cut(s) 256, 298, 306, 551, 735
BstMAI GTCTC 2 cut(s) 61, 68
BstMBI GATC 5 cut(s) 253, 295, 303, 548, 732
BstMCI CGRYCG 1 cut(s) 551
BstMWI GCNNNNNNNGC 5 cut(s) 468, 793, 832, 932, 983
BstNI CCWGG 1 cut(s) 577
BstNSI RCATGY 1 cut(s) 670
BstSCI CCNGG 1 cut(s) 575
BstSFI CTRYAG 1 cut(s) 54
BstSLI GKGCMC 1 cut(s) 828
BstV1I GCAGC 6 cut(s) 40, 68, 185, 262, 913, 970
BstX2I RGATCY 2 cut(s) 295, 732
BstXI CCANNNNNNTGG 1 cut(s) 922
BstYI RGATCY 2 cut(s) 295, 732
BsuI GTATCC 1 cut(s) 201
BsuRI GGCC 3 cut(s) 6, 40, 802
BtrI CACGTC 1 cut(s) 490
BtsCI GGATG 1 cut(s) 586
BtsI GCAGTG 1 cut(s) 972
BtsIMutI CAGTG 2 cut(s) 539, 972
Cac8I GCNNGC 3 cut(s) 27, 333, 888
Cfr10I RCCGGY 1 cut(s) 935
Cfr13I GGNCC 2 cut(s) 4, 39
Csp6I GTAC 3 cut(s) 249, 415, 481
CviAII CATG 7 cut(s) 79, 99, 472, 500, 625, 667, 832
CviQI GTAC 3 cut(s) 249, 415, 481
DdeI CTNAG 4 cut(s) 72, 213, 453, 843
DpnI GATC 5 cut(s) 255, 297, 305, 550, 734
DpnII GATC 5 cut(s) 253, 295, 303, 548, 732
DraIII CACNNNGTG 1 cut(s) 367
Eco31I GGTCTC 2 cut(s) 61, 68
EcoRII CCWGG 1 cut(s) 575
EcoT22I ATGCAT 1 cut(s) 100
FaeI CATG 7 cut(s) 82, 102, 475, 503, 628, 670, 835
FaqI GGGAC 1 cut(s) 677
FatI CATG 7 cut(s) 78, 98, 471, 499, 624, 666, 831
Fnu4HI GCNGC 6 cut(s) 54, 57, 174, 276, 927, 984
FokI GGATG 1 cut(s) 593
Fsp4HI GCNGC 6 cut(s) 54, 57, 174, 276, 927, 984
FspBI CTAG 2 cut(s) 332, 741
GluI GCNGC 6 cut(s) 54, 57, 174, 276, 927, 984
HaeIII GGCC 3 cut(s) 6, 40, 802
HapII CCGG 1 cut(s) 936
Hin1II CATG 7 cut(s) 82, 102, 475, 503, 628, 670, 835
HinfI GANTC 6 cut(s) 350, 529, 599, 611, 677, 744
HpaII CCGG 1 cut(s) 936
HphI GGTGA 3 cut(s) 392, 440, 950
Hpy166II GTNNAC 2 cut(s) 545, 861
Hpy188I TCNGA 3 cut(s) 355, 487, 610
Hpy188III TCNNGA 4 cut(s) 301, 546, 741, 790
Hpy8I GTNNAC 2 cut(s) 545, 861
HpyAV CCTTC 3 cut(s) 111, 231, 277
HpyCH4III ACNGT 1 cut(s) 375
HpyCH4IV ACGT 5 cut(s) 178, 363, 489, 515, 568
HpyCH4V TGCA 5 cut(s) 56, 98, 762, 956, 977
HpyF10VI GCNNNNNNNGC 5 cut(s) 468, 793, 832, 932, 983
HpyF3I CTNAG 4 cut(s) 72, 213, 453, 843
HpySE526I ACGT 5 cut(s) 178, 363, 489, 515, 568
Hsp92II CATG 7 cut(s) 82, 102, 475, 503, 628, 670, 835
Kzo9I GATC 5 cut(s) 253, 295, 303, 548, 732
LmnI GCTCC 1 cut(s) 125
Lsp1109I GCAGC 6 cut(s) 40, 68, 185, 262, 913, 970
LweI GCATC 3 cut(s) 901, 922, 943
MaeI CTAG 2 cut(s) 332, 741
MaeII ACGT 5 cut(s) 178, 363, 489, 515, 568
MaeIII GTNAC 2 cut(s) 369, 490
MalI GATC 5 cut(s) 255, 297, 305, 550, 734
MboI GATC 5 cut(s) 253, 295, 303, 548, 732
MboII GAAGA 1 cut(s) 859
MflI RGATCY 2 cut(s) 295, 732
MhlI GDGCHC 1 cut(s) 828
MluCI AATT 6 cut(s) 32, 260, 780, 838, 868, 958
MlyI GAGTC 1 cut(s) 608
MnlI CCTC 4 cut(s) 448, 813, 839, 889
Mph1103I ATGCAT 1 cut(s) 100
MseI TTAA 2 cut(s) 518, 752
MslI CAYNNNNRTG 1 cut(s) 920
MspI CCGG 1 cut(s) 936
MspR9I CCNGG 1 cut(s) 577
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 1 cut(s) 577
MwoI GCNNNNNNNGC 5 cut(s) 468, 793, 832, 932, 983
NdeII GATC 5 cut(s) 253, 295, 303, 548, 732
NheI GCTAGC 1 cut(s) 331
NlaIII CATG 7 cut(s) 82, 102, 475, 503, 628, 670, 835
NlaIV GGNNCC 2 cut(s) 127, 827
NmuCI GTSAC 2 cut(s) 369, 490
NsiI ATGCAT 1 cut(s) 100
NspI RCATGY 1 cut(s) 670
PasI CCCWGGG 1 cut(s) 576
PciI ACATGT 1 cut(s) 666
PctI GAATGC 1 cut(s) 464
PfeI GAWTC 5 cut(s) 350, 529, 611, 677, 744
PkrI GCNGC 6 cut(s) 55, 58, 175, 277, 928, 985
Ple19I CGATCG 1 cut(s) 551
PleI GAGTC 1 cut(s) 607
PpsI GAGTC 1 cut(s) 607
Ppu21I YACGTR 3 cut(s) 179, 364, 569
PscI ACATGT 1 cut(s) 666
Psp6I CCWGG 1 cut(s) 575
PspGI CCWGG 1 cut(s) 575
PspN4I GGNNCC 2 cut(s) 127, 827
PspPI GGNCC 2 cut(s) 4, 39
PstI CTGCAG 1 cut(s) 58
PsuI RGATCY 2 cut(s) 295, 732
PvuI CGATCG 1 cut(s) 551
RsaI GTAC 3 cut(s) 250, 416, 482
RsaNI GTAC 3 cut(s) 249, 415, 481
RseI CAYNNNNRTG 1 cut(s) 920
SaqAI TTAA 2 cut(s) 518, 752
SatI GCNGC 6 cut(s) 54, 57, 174, 276, 927, 984
Sau3AI GATC 5 cut(s) 253, 295, 303, 548, 732
Sau96I GGNCC 2 cut(s) 4, 39
SchI GAGTC 1 cut(s) 608
ScrFI CCNGG 1 cut(s) 577
SduI GDGCHC 1 cut(s) 828
SfaNI GCATC 3 cut(s) 901, 922, 943
SfcI CTRYAG 1 cut(s) 54
SmiMI CAYNNNNRTG 1 cut(s) 920
SmlI CTYRAG 2 cut(s) 299, 788
SmoI CTYRAG 2 cut(s) 299, 788
Sse9I AATT 6 cut(s) 32, 260, 780, 838, 868, 958
SsiI CCGC 1 cut(s) 278
SspI AATATT 1 cut(s) 902
SspMI CTAG 2 cut(s) 332, 741
StyD4I CCNGG 1 cut(s) 575
TaaI ACNGT 1 cut(s) 375
TaiI ACGT 5 cut(s) 181, 366, 492, 518, 571
TaqI TCGA 1 cut(s) 467
TaqII GACCGA 1 cut(s) 728
TasI AATT 6 cut(s) 32, 260, 780, 838, 868, 958
TatI WGTACW 1 cut(s) 480
TfiI GAWTC 5 cut(s) 350, 529, 611, 677, 744
Tru1I TTAA 2 cut(s) 518, 752
Tru9I TTAA 2 cut(s) 518, 752
TscAI CASTG 2 cut(s) 539, 979
TseFI GTSAC 2 cut(s) 369, 490
TseI GCWGC 6 cut(s) 53, 56, 173, 275, 926, 983
Tsp45I GTSAC 2 cut(s) 369, 490
TspDTI ATGAA 5 cut(s) 156, 220, 399, 488, 597
TspRI CASTG 2 cut(s) 539, 979
XapI RAATTY 3 cut(s) 32, 868, 958
XbaI TCTAGA 1 cut(s) 740
XceI RCATGY 1 cut(s) 670
XspI CTAG 2 cut(s) 332, 741
Zsp2I ATGCAT 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.