Prupe.4G284700_v2.0.a1

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
N/A
Physical Location & Seq
Forward (+)
24678427 .. 24681589
3163 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G284700.1_v2.0.a1.CDS.2

Sequence Viewer

Length: 972 bp
ATGAAGGTATTGAAGAAAGATTCTGCTACTTTTGAGGAAAGATTTACGGGACAGGTGTGGTTTCAGTGCGTGTTTGCAGCCATTACGCTGATACTGCTGGCGGGGTCGGTGCTTGGGAGGATGAACTTCAAGGCGTGGATGATGTTCGTGCCGCTTTGGCTTACATTTTCGTACACTGTCGGAGCTTTCAGCTTGTGGGGTGGAGGGTTCTTGTTCCATTGGGGTGTCATGGACTATTCCGGTGGTTATGTCATTCATCTTTCTTCCGGGATTGCTGTCTTCACTACTGCTTACTGGGTAGGACCAAGGGTGAAGAAGGACAGGGAGAGATTTCCACCAAACAACGTACTTCTGGTGTTAGCAGGAGCCGGGTTGCTGTGGATGGGATGGGCAGGTTTCAACGGGGGAGACCCGTACGCGGCAAACACGGACTCATCCATGGCCGTCCTCAACACCAACATCTGCGCCGCCACGAGCTTCTTGGTTTGGACTTGGCTCGATGTCATCTTCTTTGAGAAGCCTTCCGTCATTGGAGCTGTCCAAGGAATGATCACTGGCCTCGTCTGCATTACTCCTGCTGCAGGTCTGGTTCAAGGATGGGCCGCTATAGTGATGGGAGTTATGTCAGGCAGTGTGCCATGGTTCACCATGATGATAGTGGACAAGAGGTGGAAACTGCTATCTGCAGTTGACGACACCCTTGGTGTAGTTCACACCCACGCCGTGGCCGGGTTTCTTGGCGGCGTTTTAACGGGGCTTTTCGCTGAGCCTGAACTATTCTTACGGTGTATAGTTCCGTTGCGCATGCCGGAGGAACAGTTACTGATTGGGGATGACGCAGTGCATGGAGAAGAGGCATATGCATTGTGGGGTGATGGGGAGAAGTATGATGTTACTAGGCATGAGCTTTACTCCGACGATACGTCACACGCTCAAAAATATCCATCCAGTGGTGCCACTCAGGTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

324

Amino Acids

35.36

Weight (kDa)

5.46

Isoelectric Point (pI)

27.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 803
Acc36I ACCTGC 2 cut(s) 383, 572
AccB1I GGYRCC 1 cut(s) 953
AccB7I CCANNNNNTGG 2 cut(s) 724, 950
AccII CGCG 1 cut(s) 419
AciI CCGC 6 cut(s) 101, 152, 419, 468, 603, 741
AcoI YGGCCR 2 cut(s) 441, 726
AdeI CACNNNGTG 1 cut(s) 724
AfaI GTAC 3 cut(s) 173, 348, 416
AfiI CCNNNNNNNGG 5 cut(s) 418, 581, 724, 729, 950
AgsI TTSAA 4 cut(s) 13, 130, 400, 593
AhdI GACNNNNNGTC 1 cut(s) 922
AleI CACNNNNGTG 1 cut(s) 962
AluBI AGCT 5 cut(s) 185, 192, 477, 536, 907
AluI AGCT 5 cut(s) 185, 192, 477, 536, 907
Alw26I GTCTC 1 cut(s) 402
AlwNI CAGNNNCTG 1 cut(s) 823
AoxI GGCC 4 cut(s) 441, 556, 600, 726
ApeKI GCWGC 2 cut(s) 77, 578
AspLEI GCGC 2 cut(s) 467, 804
AspS9I GGNCC 2 cut(s) 302, 600
AsuC2I CCSGG 3 cut(s) 268, 370, 730
AsuHPI GGTGA 3 cut(s) 322, 637, 884
AvaII GGWCC 1 cut(s) 302
BanI GGYRCC 1 cut(s) 953
BauI CACGAG 1 cut(s) 472
BbsI GAAGAC 1 cut(s) 271
BbvI GCAGC 2 cut(s) 89, 565
BccI CCATC 6 cut(s) 376, 381, 591, 607, 869, 952
BceAI ACGGC 2 cut(s) 428, 707
BclI TGATCA 1 cut(s) 549
BcnI CCSGG 3 cut(s) 268, 370, 730
BcoDI GTCTC 1 cut(s) 402
BfaI CTAG 1 cut(s) 897
BfmI CTRYAG 3 cut(s) 579, 606, 684
BfuAI ACCTGC 2 cut(s) 383, 572
BglI GCCNNNNNGGC 1 cut(s) 157
BisI GCNGC 7 cut(s) 78, 152, 420, 468, 579, 603, 742
BlpI GCTNAGC 1 cut(s) 765
BlsI GCNGC 7 cut(s) 79, 153, 421, 469, 580, 604, 743
Bme1390I CCNGG 3 cut(s) 268, 370, 730
Bme18I GGWCC 1 cut(s) 302
BmeRI GACNNNNNGTC 1 cut(s) 922
BmgT120I GGNCC 2 cut(s) 302, 600
BmiI GGNNCC 2 cut(s) 367, 955
BmrFI CCNGG 3 cut(s) 268, 370, 730
BmrI ACTGGG 1 cut(s) 304
BmuI ACTGGG 1 cut(s) 304
BpiI GAAGAC 1 cut(s) 271
BplI GAGNNNNNCTC 2 cut(s) 896, 928
Bpu1102I GCTNAGC 1 cut(s) 765
BpuMI CCSGG 3 cut(s) 268, 370, 730
BsaI GGTCTC 1 cut(s) 402
BsaJI CCNNGG 6 cut(s) 305, 438, 541, 638, 700, 723
BsaWI WCCGGW 1 cut(s) 239
BsaXI ACNNNNNCTCC 2 cut(s) 803, 833
Bsc4I CCNNNNNNNGG 5 cut(s) 418, 581, 724, 729, 950
Bse1I ACTGG 3 cut(s) 299, 559, 948
BseDI CCNNGG 6 cut(s) 305, 438, 541, 638, 700, 723
BseGI GGATG 8 cut(s) 126, 144, 387, 392, 434, 602, 838, 944
BseLI CCNNNNNNNGG 5 cut(s) 418, 581, 724, 729, 950
BseMII CTCAG 1 cut(s) 756
BseNI ACTGG 3 cut(s) 299, 559, 948
BseXI GCAGC 2 cut(s) 89, 565
Bsh1236I CGCG 1 cut(s) 419
BshFI GGCC 4 cut(s) 443, 558, 602, 728
BshNI GGYRCC 1 cut(s) 953
BsiSI CCGG 5 cut(s) 240, 267, 369, 729, 809
BsiWI CGTACG 1 cut(s) 414
BslFI GGGAC 1 cut(s) 63
BslI CCNNNNNNNGG 5 cut(s) 418, 581, 724, 729, 950
BsmAI GTCTC 1 cut(s) 402
BsmFI GGGAC 1 cut(s) 63
BsnI GGCC 4 cut(s) 443, 558, 602, 728
Bso31I GGTCTC 1 cut(s) 402
Bsp143I GATC 1 cut(s) 549
Bsp1720I GCTNAGC 1 cut(s) 765
Bsp19I CCATGG 2 cut(s) 438, 638
BspACI CCGC 6 cut(s) 101, 152, 419, 468, 603, 741
BspANI GGCC 4 cut(s) 443, 558, 602, 728
BspCNI CTCAG 1 cut(s) 757
BspFNI CGCG 1 cut(s) 419
BspLI GGNNCC 2 cut(s) 367, 955
BspMAI CTGCAG 2 cut(s) 583, 688
BspMI ACCTGC 2 cut(s) 383, 572
BspT107I GGYRCC 1 cut(s) 953
BspTNI GGTCTC 1 cut(s) 402
BsrI ACTGG 3 cut(s) 299, 559, 948
BssECI CCNNGG 6 cut(s) 305, 438, 541, 638, 700, 723
BssMI GATC 1 cut(s) 549
BssSI CACGAG 1 cut(s) 472
BssT1I CCWWGG 5 cut(s) 305, 438, 541, 638, 700
Bst2BI CACGAG 1 cut(s) 472
Bst4CI ACNGT 3 cut(s) 178, 786, 819
Bst6I CTCTTC 1 cut(s) 846
BstC8I GCNNGC 2 cut(s) 99, 806
BstDEI CTNAG 2 cut(s) 765, 960
BstDSI CCRYGG 3 cut(s) 438, 638, 723
BstENI CCTNNNNNAGG 1 cut(s) 579
BstF5I GGATG 8 cut(s) 126, 144, 387, 392, 434, 602, 838, 944
BstFNI CGCG 1 cut(s) 419
BstHHI GCGC 2 cut(s) 467, 804
BstKTI GATC 1 cut(s) 552
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 1 cut(s) 549
BstMWI GCNNNNNNNGC 3 cut(s) 94, 157, 564
BstNSI RCATGY 1 cut(s) 808
BstSCI CCNGG 3 cut(s) 266, 368, 728
BstSFI CTRYAG 3 cut(s) 579, 606, 684
BstUI CGCG 1 cut(s) 419
BstV1I GCAGC 2 cut(s) 89, 565
BstV2I GAAGAC 1 cut(s) 271
BstXI CCANNNNNNTGG 1 cut(s) 964
BsuRI GGCC 4 cut(s) 443, 558, 602, 728
BtgI CCRYGG 3 cut(s) 438, 638, 723
BtsCI GGATG 8 cut(s) 126, 144, 387, 392, 434, 602, 838, 944
BtsI GCAGTG 2 cut(s) 637, 846
BtsIMutI CAGTG 6 cut(s) 71, 174, 552, 637, 846, 955
BveI ACCTGC 2 cut(s) 383, 572
Cac8I GCNNGC 2 cut(s) 99, 806
CaiI CAGNNNCTG 1 cut(s) 823
CfoI GCGC 2 cut(s) 467, 804
Cfr13I GGNCC 2 cut(s) 302, 600
CseI GACGC 1 cut(s) 845
Csp6I GTAC 3 cut(s) 172, 347, 415
CviAII CATG 7 cut(s) 229, 439, 639, 649, 805, 845, 902
CviQI GTAC 3 cut(s) 172, 347, 415
DdeI CTNAG 2 cut(s) 765, 960
DpnI GATC 1 cut(s) 551
DpnII GATC 1 cut(s) 549
DraIII CACNNNGTG 1 cut(s) 724
DriI GACNNNNNGTC 1 cut(s) 922
EaeI YGGCCR 2 cut(s) 441, 726
Eam1104I CTCTTC 1 cut(s) 846
Eam1105I GACNNNNNGTC 1 cut(s) 922
EarI CTCTTC 1 cut(s) 846
Eco130I CCWWGG 5 cut(s) 305, 438, 541, 638, 700
Eco31I GGTCTC 1 cut(s) 402
Eco47I GGWCC 1 cut(s) 302
EcoNI CCTNNNNNAGG 1 cut(s) 579
EcoT14I CCWWGG 5 cut(s) 305, 438, 541, 638, 700
EcoT22I ATGCAT 1 cut(s) 865
ErhI CCWWGG 5 cut(s) 305, 438, 541, 638, 700
FaeI CATG 7 cut(s) 232, 442, 642, 652, 808, 848, 905
FaqI GGGAC 1 cut(s) 63
FatI CATG 7 cut(s) 228, 438, 638, 648, 804, 844, 901
FauI CCCGC 1 cut(s) 94
FauNDI CATATG 1 cut(s) 859
FbaI TGATCA 1 cut(s) 549
Fnu4HI GCNGC 7 cut(s) 78, 152, 420, 468, 579, 603, 742
FokI GGATG 8 cut(s) 133, 151, 394, 399, 421, 609, 845, 931
Fsp4HI GCNGC 7 cut(s) 78, 152, 420, 468, 579, 603, 742
FspBI CTAG 1 cut(s) 897
FspI TGCGCA 1 cut(s) 803
GlaI GCGC 2 cut(s) 466, 803
GluI GCNGC 7 cut(s) 78, 152, 420, 468, 579, 603, 742
HaeIII GGCC 4 cut(s) 443, 558, 602, 728
HapII CCGG 5 cut(s) 240, 267, 369, 729, 809
HgaI GACGC 1 cut(s) 845
HhaI GCGC 2 cut(s) 467, 804
Hin1II CATG 7 cut(s) 232, 442, 642, 652, 808, 848, 905
Hin6I GCGC 2 cut(s) 465, 802
HinP1I GCGC 2 cut(s) 465, 802
HincII GTYRAC 1 cut(s) 691
HindII GTYRAC 1 cut(s) 691
HinfI GANTC 2 cut(s) 20, 431
HpaII CCGG 5 cut(s) 240, 267, 369, 729, 809
HphI GGTGA 3 cut(s) 322, 637, 884
Hpy166II GTNNAC 5 cut(s) 174, 645, 661, 691, 712
Hpy188I TCNGA 2 cut(s) 182, 916
Hpy8I GTNNAC 5 cut(s) 174, 645, 661, 691, 712
Hpy99I CGWCG 1 cut(s) 920
HpyAV CCTTC 2 cut(s) 310, 531
HpyCH4III ACNGT 3 cut(s) 178, 786, 819
HpyCH4IV ACGT 2 cut(s) 345, 923
HpyCH4V TGCA 6 cut(s) 77, 567, 581, 686, 844, 863
HpyF10VI GCNNNNNNNGC 3 cut(s) 94, 157, 564
HpyF3I CTNAG 2 cut(s) 765, 960
HpySE526I ACGT 2 cut(s) 345, 923
Hsp92II CATG 7 cut(s) 232, 442, 642, 652, 808, 848, 905
HspAI GCGC 2 cut(s) 465, 802
Ksp22I TGATCA 1 cut(s) 549
Kzo9I GATC 1 cut(s) 549
LmnI GCTCC 3 cut(s) 182, 365, 533
Lsp1109I GCAGC 2 cut(s) 89, 565
MaeI CTAG 1 cut(s) 897
MaeII ACGT 2 cut(s) 345, 923
MaeIII GTNAC 3 cut(s) 819, 892, 924
MalI GATC 1 cut(s) 551
MboI GATC 1 cut(s) 549
MboII GAAGA 6 cut(s) 25, 255, 271, 325, 499, 863
MlyI GAGTC 1 cut(s) 425
MmeI TCCRAC 2 cut(s) 160, 939
MnlI CCTC 8 cut(s) 28, 111, 197, 458, 569, 660, 805, 847
Mph1103I ATGCAT 1 cut(s) 865
MseI TTAA 1 cut(s) 749
MslI CAYNNNNRTG 3 cut(s) 222, 650, 962
MspI CCGG 5 cut(s) 240, 267, 369, 729, 809
MspR9I CCNGG 3 cut(s) 268, 370, 730
MvnI CGCG 1 cut(s) 419
MwoI GCNNNNNNNGC 3 cut(s) 94, 157, 564
NciI CCSGG 3 cut(s) 268, 370, 730
NcoI CCATGG 2 cut(s) 438, 638
NdeI CATATG 1 cut(s) 859
NdeII GATC 1 cut(s) 549
NlaIII CATG 7 cut(s) 232, 442, 642, 652, 808, 848, 905
NlaIV GGNNCC 2 cut(s) 367, 955
NmuCI GTSAC 1 cut(s) 924
NsbI TGCGCA 1 cut(s) 803
NsiI ATGCAT 1 cut(s) 865
NspI RCATGY 1 cut(s) 808
OliI CACNNNNGTG 1 cut(s) 962
PaeI GCATGC 1 cut(s) 808
PfeI GAWTC 1 cut(s) 20
Pfl23II CGTACG 1 cut(s) 414
PflMI CCANNNNNTGG 2 cut(s) 724, 950
PfoI TCCNGGA 1 cut(s) 266
PkrI GCNGC 7 cut(s) 79, 153, 421, 469, 580, 604, 743
PleI GAGTC 1 cut(s) 425
PpsI GAGTC 1 cut(s) 425
PspLI CGTACG 1 cut(s) 414
PspN4I GGNNCC 2 cut(s) 367, 955
PspPI GGNCC 2 cut(s) 302, 600
PsrI GAACNNNNNNTAC 2 cut(s) 765, 797
PstI CTGCAG 2 cut(s) 583, 688
PstNI CAGNNNCTG 1 cut(s) 823
RsaI GTAC 3 cut(s) 173, 348, 416
RsaNI GTAC 3 cut(s) 172, 347, 415
RseI CAYNNNNRTG 3 cut(s) 222, 650, 962
SaqAI TTAA 1 cut(s) 749
SatI GCNGC 7 cut(s) 78, 152, 420, 468, 579, 603, 742
Sau3AI GATC 1 cut(s) 549
Sau96I GGNCC 2 cut(s) 302, 600
SchI GAGTC 1 cut(s) 425
ScrFI CCNGG 3 cut(s) 268, 370, 730
SfcI CTRYAG 3 cut(s) 579, 606, 684
SinI GGWCC 1 cut(s) 302
SmiMI CAYNNNNRTG 3 cut(s) 222, 650, 962
SphI GCATGC 1 cut(s) 808
SsiI CCGC 6 cut(s) 101, 152, 419, 468, 603, 741
SspMI CTAG 1 cut(s) 897
StyD4I CCNGG 3 cut(s) 266, 368, 728
StyI CCWWGG 5 cut(s) 305, 438, 541, 638, 700
TaaI ACNGT 3 cut(s) 178, 786, 819
TaiI ACGT 2 cut(s) 348, 926
TaqI TCGA 1 cut(s) 498
TauI GCSGC 5 cut(s) 154, 422, 470, 605, 744
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 1 cut(s) 749
Tru9I TTAA 1 cut(s) 749
TscAI CASTG 6 cut(s) 71, 181, 559, 637, 846, 955
TseFI GTSAC 1 cut(s) 924
TseI GCWGC 2 cut(s) 77, 578
Tsp45I GTSAC 1 cut(s) 924
TspDTI ATGAA 3 cut(s) 17, 137, 245
TspGWI ACGGA 3 cut(s) 443, 514, 786
TspRI CASTG 6 cut(s) 71, 181, 559, 637, 846, 955
Van91I CCANNNNNTGG 2 cut(s) 724, 950
VpaK11BI GGWCC 1 cut(s) 302
XagI CCTNNNNNAGG 1 cut(s) 579
XceI RCATGY 1 cut(s) 808
XcmI CCANNNNNNNNNTGG 2 cut(s) 478, 655
XspI CTAG 1 cut(s) 897
Zsp2I ATGCAT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.