Prupe.5G084600_v2.0.a1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
N/A
Physical Location & Seq
Forward (+)
9702069 .. 9704771
2703 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G084600.1_v2.0.a1.CDS.2

Sequence Viewer

Length: 1947 bp
ATGGAGAAGAAAGAAGAGGTTCTTTCTATTCATAACGAGACAAATTTTTTTTTGCAAACAACTCATAGTCCTGGATTTCTGGGCTTGCACCCAGAGAAGGGGTTCTGGAAAAAATCAAATTTCGGCAAGGGTGTAACTATTGGTGTTATGGACACTGGCGTGTTACCAGATCATCCTTCGTTTCGGGATGAAGGAATGCCTCCTCCACCAGATAGATGGAAAGGCAGGTGTGACTTCAACCAGACAGCTAATCGCACATTCGTCTGCAACAACAAGATCATTGGTGCAAGAAGTTTCCAAGTCTATGACAACCTTTTACAGACTTCTAGTGCTCAACCAGTTGATGAGATAGGCCATGGCACTCATACTGCTAGCACGGCTGCTGGGAATTTTGTGAGAGGTGCCAATCTATATAACAATGCCAATGGCATGGCTTCTGGCGTAGCGCCTCATGCTCACTTGGCAATTTACCAGGTCTGCTCTCTTGATGAATGTTCGAGTACTGATGTCCTTGCAGGATTTGATGCTGCAGTCGAGGATGGTGTCGATGTGATTTCCATCTCCATGACCTCTCTAAATGTCGGTCAACTGTTTGAAGACAGTATTGCATTGGCTGCTTTCAGTGCAATGCAAAGAAGAATTTTTGTGGTTTCAAGCGCCATTGATAGAAGCATAATAGCAACAGCTAGACTTGGAAATGGGGATAAATTTGATGGGGAGTCAATTTTTCAGCCTAACTCTACTAAAGGGTTAAACTCCAAATTGTTGCCTTTGGTTCAACCAGGCATTAATGGTCAGATGCTTTCTCAAAACTGTAACAATGGGTCACTGAAAGATATTAATGTGGAAGGAAAAGTAGTTTTCTGTGAGAGAGGTATGGAAATAAGTAGACTGGATCAAGGAAAAGTAGTGAAAGCTGCAGGTGGAGCCGCCATGCTTCTTGTGAACCAAGAACAGGAGGGCTTTACGACCTACACTGACCCCCATGTTCTTCCTGCATCCCATTTGAGTTATGCTGCAGGCCTGAACATCAAGCACTATATAAACAAAACCTCTAAATTGATAGCTACAGTCAAATTCAAAGGAACTGTAACTGGAAACTCATCTGCTCCCATGGTGGCTTCCTTCTCCTCTAGGGGACCAAGCTCCATATGTCCCGGGATTTTGAAGCCTGACATTATTGGACCGGGAGTGAGCATTCTAGCAGCCTCACCTTTCCCTATTGACAAGAGGAAGTCGAATTCGAAATCAAACTTCAATGTGATGTCAGGTACCTCAATGTCATGTCCTCACCTGAGTGGCATTGCAGCTTTGCTAAAGAGTTCCCACCCTGACTGGTCACCTGCTGCTATTAAGTGTGCAATCATGACTACAGCTGAGGTTGTAAACCAGAAAGGCCAGCCAATCACTGATGAAACACTCCAGCCAGCCAGTGTCTTTGCCATTGGCTCAGACCATCCAAACTCATCAAGGGCAAATGATCCGGGTCTCGTCTATGACATCAAACCCCTTGATTACATACCTTACTTGTGCGGTAAGAATTACACTGACAGACATGTTGGGGTTCTGTTGCAAGGCAAGGTGAACTGCTCTTTGGAAAAACCCATATCTGAAGCTCAACTAAATTATCCTTCATTTTCCATTGTATTGGGGCTCACTCCTCAGACTTATACAAGAGAGGTAACAAACGTTGGCAAGGCCGATTCTTCAGACACTGTGAAGGTTGCTCCACCCCAAGGAATCAGTGTCAGCATCAAGCCTAAACTTATTACATTCTCAGAGATGAATCAGAAGGCAAAATATGCGGTTACTTTTAGACGAAGCAAGGATGGAGGAAAATCAGATAAAACTGCACTAGTTGTTCAGTATGCTGAGGGATCTCTGACATGGGTTTCTGATCAATATTCAGTTAGGAGCCCAATAGCTATCAAATTACCATTAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

649

Amino Acids

69.49

Weight (kDa)

8.33

Isoelectric Point (pI)

32.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 216, 911, 1351
Acc36I ACCTGC 3 cut(s) 216, 911, 1351
Acc65I GGTACC 1 cut(s) 1271
AccB1I GGYRCC 2 cut(s) 401, 1271
AccI GTMKAC 1 cut(s) 889
AciI CCGC 3 cut(s) 930, 1533, 1805
AclI AACGTT 1 cut(s) 1689
AclWI GGATC 3 cut(s) 903, 1475, 1885
AcsI RAATTY 7 cut(s) 43, 118, 388, 639, 707, 1076, 1240
AcuI CTGAAG 2 cut(s) 1632, 1692
AfaI GTAC 2 cut(s) 502, 1273
AfiI CCNNNNNNNGG 4 cut(s) 97, 98, 955, 1736
AflIII ACRYGT 1 cut(s) 1555
AgsI TTSAA 7 cut(s) 238, 596, 654, 779, 1081, 1168, 1258
AhlI ACTAGT 1 cut(s) 1855
AjnI CCWGG 3 cut(s) 70, 471, 781
AjuI GAANNNNNNNTTGG 2 cut(s) 1577, 1609
AleI CACNNNNGTG 2 cut(s) 158, 1296
AluBI AGCT 9 cut(s) 248, 686, 917, 1067, 1146, 1310, 1376, 1616, 1925
AluI AGCT 9 cut(s) 248, 686, 917, 1067, 1146, 1310, 1376, 1616, 1925
Alw21I GWGCWC 1 cut(s) 334
Alw26I GTCTC 2 cut(s) 32, 1493
AlwI GGATC 3 cut(s) 903, 1475, 1885
AlwNI CAGNNNCTG 1 cut(s) 1715
Ama87I CYCGRG 1 cut(s) 1157
AoxI GGCC 4 cut(s) 352, 1021, 1396, 1698
ApeKI GCWGC 8 cut(s) 380, 527, 614, 917, 1016, 1205, 1307, 1346
ApoI RAATTY 7 cut(s) 43, 118, 388, 639, 707, 1076, 1240
AseI ATTAAT 2 cut(s) 789, 840
Asp700I GAANNNNTTC 2 cut(s) 18, 101
Asp718I GGTACC 1 cut(s) 1271
AspLEI GCGC 2 cut(s) 448, 659
AspS9I GGNCC 2 cut(s) 1139, 1184
AsuC2I CCSGG 4 cut(s) 1158, 1159, 1188, 1485
AsuHPI GGTGA 4 cut(s) 1203, 1283, 1332, 1594
AsuII TTCGAA 1 cut(s) 1244
AsuNHI GCTAGC 1 cut(s) 371
AvaI CYCGRG 1 cut(s) 1157
AvaII GGWCC 2 cut(s) 1139, 1184
BanI GGYRCC 2 cut(s) 401, 1271
BanII GRGCYC 2 cut(s) 1656, 1919
BbsI GAAGAC 1 cut(s) 603
Bbv12I GWGCWC 1 cut(s) 334
BbvCI CCTCAGC 2 cut(s) 1377, 1872
BbvI GCAGC 8 cut(s) 367, 514, 601, 904, 1003, 1217, 1319, 1333
BccI CCATC 6 cut(s) 210, 533, 566, 707, 1464, 1823
BceAI ACGGC 1 cut(s) 393
BciT130I CCWGG 3 cut(s) 72, 473, 783
BclI TGATCA 1 cut(s) 1897
BcnI CCSGG 4 cut(s) 1158, 1159, 1188, 1485
BcoDI GTCTC 2 cut(s) 32, 1493
BcuI ACTAGT 1 cut(s) 1855
BfaI CTAG 6 cut(s) 327, 372, 687, 1134, 1202, 1856
BfmI CTRYAG 5 cut(s) 528, 918, 1017, 1068, 1371
BfoI RGCGCY 2 cut(s) 449, 660
BfuAI ACCTGC 3 cut(s) 216, 911, 1351
BisI GCNGC 9 cut(s) 381, 528, 615, 918, 930, 1017, 1206, 1308, 1347
BlsI GCNGC 9 cut(s) 382, 529, 616, 919, 931, 1018, 1207, 1309, 1348
BmcAI AGTACT 1 cut(s) 502
Bme1390I CCNGG 7 cut(s) 72, 473, 783, 1158, 1159, 1188, 1485
Bme18I GGWCC 2 cut(s) 1139, 1184
BmeT110I CYCGRG 1 cut(s) 1157
BmgT120I GGNCC 2 cut(s) 1139, 1184
BmiI GGNNCC 5 cut(s) 403, 928, 1140, 1273, 1916
BmrFI CCNGG 7 cut(s) 72, 473, 783, 1158, 1159, 1188, 1485
BmsI GCATC 4 cut(s) 514, 789, 1007, 1761
BmtI GCTAGC 1 cut(s) 375
BpiI GAAGAC 1 cut(s) 603
BpmI CTGGAG 1 cut(s) 1406
Bpu10I CCTNAGC 2 cut(s) 1377, 1872
Bpu14I TTCGAA 1 cut(s) 1244
BpuMI CCSGG 4 cut(s) 1158, 1159, 1188, 1485
BsaBI GATNNNNATC 1 cut(s) 557
BsaI GGTCTC 1 cut(s) 1493
BsaJI CCNNGG 4 cut(s) 355, 1113, 1157, 1735
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 98, 955, 1736
Bse1I ACTGG 6 cut(s) 160, 338, 897, 1099, 1340, 1431
Bse3DI GCAATG 2 cut(s) 633, 1302
Bse8I GATNNNNATC 1 cut(s) 557
BseBI CCWGG 3 cut(s) 72, 473, 783
BseDI CCNNGG 4 cut(s) 355, 1113, 1157, 1735
BseGI GGATG 6 cut(s) 172, 193, 544, 998, 1456, 1834
BseJI GATNNNNATC 1 cut(s) 557
BseLI CCNNNNNNNGG 4 cut(s) 97, 98, 955, 1736
BseMI GCAATG 2 cut(s) 633, 1302
BseMII CTCAG 6 cut(s) 1286, 1368, 1464, 1676, 1791, 1863
BseNI ACTGG 6 cut(s) 160, 338, 897, 1099, 1340, 1431
BseRI GAGGAG 3 cut(s) 192, 1120, 1650
BseXI GCAGC 8 cut(s) 367, 514, 601, 904, 1003, 1217, 1319, 1333
BseYI CCCAGC 1 cut(s) 383
BsgI GTGCAG 1 cut(s) 1836
BshFI GGCC 4 cut(s) 354, 1023, 1398, 1700
BshNI GGYRCC 2 cut(s) 401, 1271
BsiHKAI GWGCWC 1 cut(s) 334
BsiHKCI CYCGRG 1 cut(s) 1157
BsiSI CCGG 3 cut(s) 1158, 1187, 1484
BslFI GGGAC 2 cut(s) 1140, 1152
BslI CCNNNNNNNGG 4 cut(s) 97, 98, 955, 1736
BsmAI GTCTC 2 cut(s) 32, 1493
BsmFI GGGAC 2 cut(s) 1140, 1152
BsmI GAATGC 2 cut(s) 201, 1197
BsnI GGCC 4 cut(s) 354, 1023, 1398, 1700
Bso31I GGTCTC 1 cut(s) 1493
BsoBI CYCGRG 1 cut(s) 1157
Bsp119I TTCGAA 1 cut(s) 1244
Bsp1286I GDGCHC 3 cut(s) 334, 1656, 1919
Bsp143I GATC 6 cut(s) 169, 276, 895, 1480, 1877, 1897
Bsp19I CCATGG 2 cut(s) 355, 1113
BspACI CCGC 3 cut(s) 930, 1533, 1805
BspANI GGCC 4 cut(s) 354, 1023, 1398, 1700
BspCNI CTCAG 6 cut(s) 1287, 1369, 1463, 1675, 1790, 1864
BspHI TCATGA 1 cut(s) 1365
BspLI GGNNCC 5 cut(s) 403, 928, 1140, 1273, 1916
BspMAI CTGCAG 3 cut(s) 532, 922, 1021
BspMI ACCTGC 3 cut(s) 216, 911, 1351
BspOI GCTAGC 1 cut(s) 375
BspPI GGATC 3 cut(s) 903, 1475, 1885
BspT104I TTCGAA 1 cut(s) 1244
BspT107I GGYRCC 2 cut(s) 401, 1271
BspTNI GGTCTC 1 cut(s) 1493
BsrDI GCAATG 2 cut(s) 633, 1302
BsrI ACTGG 6 cut(s) 160, 338, 897, 1099, 1340, 1431
BssECI CCNNGG 4 cut(s) 355, 1113, 1157, 1735
BssMI GATC 6 cut(s) 169, 276, 895, 1480, 1877, 1897
BssT1I CCWWGG 3 cut(s) 355, 1113, 1735
Bst2UI CCWGG 3 cut(s) 72, 473, 783
Bst4CI ACNGT 6 cut(s) 591, 602, 815, 1072, 1090, 1717
Bst6I CTCTTC 1 cut(s) 9
BstAPI GCANNNNNTGC 2 cut(s) 614, 1802
BstBI TTCGAA 1 cut(s) 1244
BstC8I GCNNGC 5 cut(s) 86, 373, 1021, 1400, 1428
BstDEI CTNAG 6 cut(s) 1295, 1377, 1450, 1662, 1777, 1872
BstDSI CCRYGG 2 cut(s) 355, 1113
BstEII GGTNACC 1 cut(s) 1338
BstF5I GGATG 6 cut(s) 172, 193, 544, 998, 1456, 1834
BstH2I RGCGCY 2 cut(s) 449, 660
BstHHI GCGC 2 cut(s) 448, 659
BstKTI GATC 6 cut(s) 172, 279, 898, 1483, 1880, 1900
BstMAI GTCTC 2 cut(s) 32, 1493
BstMBI GATC 6 cut(s) 169, 276, 895, 1480, 1877, 1897
BstMWI GCNNNNNNNGC 7 cut(s) 377, 452, 461, 614, 623, 926, 1802
BstNI CCWGG 3 cut(s) 72, 473, 783
BstNSI RCATGY 1 cut(s) 1559
BstPI GGTNACC 1 cut(s) 1338
BstSCI CCNGG 7 cut(s) 70, 471, 781, 1156, 1157, 1186, 1483
BstSFI CTRYAG 5 cut(s) 528, 918, 1017, 1068, 1371
BstV1I GCAGC 8 cut(s) 367, 514, 601, 904, 1003, 1217, 1319, 1333
BstV2I GAAGAC 1 cut(s) 603
BstX2I RGATCY 1 cut(s) 1877
BstXI CCANNNNNNTGG 3 cut(s) 216, 430, 1648
BstYI RGATCY 1 cut(s) 1877
BsuRI GGCC 4 cut(s) 354, 1023, 1398, 1700
BtgI CCRYGG 2 cut(s) 355, 1113
BtsCI GGATG 6 cut(s) 172, 193, 544, 998, 1456, 1834
BtsIMutI CAGTG 9 cut(s) 153, 628, 827, 975, 1407, 1438, 1545, 1713, 1750
BveI ACCTGC 3 cut(s) 216, 911, 1351
Cac8I GCNNGC 5 cut(s) 86, 373, 1021, 1400, 1428
CaiI CAGNNNCTG 1 cut(s) 1715
CciI TCATGA 1 cut(s) 1365
CfoI GCGC 2 cut(s) 448, 659
Cfr13I GGNCC 2 cut(s) 1139, 1184
Cfr9I CCCGGG 1 cut(s) 1157
CsiI ACCWGGT 1 cut(s) 471
Csp6I GTAC 2 cut(s) 501, 1272
CviQI GTAC 2 cut(s) 501, 1272
DdeI CTNAG 6 cut(s) 1295, 1377, 1450, 1662, 1777, 1872
DpnI GATC 6 cut(s) 171, 278, 897, 1482, 1879, 1899
DpnII GATC 6 cut(s) 169, 276, 895, 1480, 1877, 1897
Eam1104I CTCTTC 1 cut(s) 9
EarI CTCTTC 1 cut(s) 9
Eco130I CCWWGG 3 cut(s) 355, 1113, 1735
Eco147I AGGCCT 1 cut(s) 1023
Eco24I GRGCYC 2 cut(s) 1656, 1919
Eco31I GGTCTC 1 cut(s) 1493
Eco47I GGWCC 2 cut(s) 1139, 1184
Eco57I CTGAAG 2 cut(s) 1632, 1692
Eco88I CYCGRG 1 cut(s) 1157
Eco91I GGTNACC 1 cut(s) 1338
EcoO65I GGTNACC 1 cut(s) 1338
EcoRI GAATTC 1 cut(s) 1240
EcoRII CCWGG 3 cut(s) 70, 471, 781
EcoT14I CCWWGG 3 cut(s) 355, 1113, 1735
EcoT38I GRGCYC 2 cut(s) 1656, 1919
ErhI CCWWGG 3 cut(s) 355, 1113, 1735
FalI AAGNNNNNCTT 1 cut(s) 38
FaqI GGGAC 2 cut(s) 1140, 1152
FauNDI CATATG 1 cut(s) 1151
FbaI TGATCA 1 cut(s) 1897
FblI GTMKAC 1 cut(s) 889
Fnu4HI GCNGC 9 cut(s) 381, 528, 615, 918, 930, 1017, 1206, 1308, 1347
FokI GGATG 6 cut(s) 159, 200, 551, 985, 1443, 1841
FriOI GRGCYC 2 cut(s) 1656, 1919
Fsp4HI GCNGC 9 cut(s) 381, 528, 615, 918, 930, 1017, 1206, 1308, 1347
FspBI CTAG 6 cut(s) 327, 372, 687, 1134, 1202, 1856
GlaI GCGC 2 cut(s) 447, 658
GluI GCNGC 9 cut(s) 381, 528, 615, 918, 930, 1017, 1206, 1308, 1347
GsaI CCCAGC 1 cut(s) 387
GsuI CTGGAG 1 cut(s) 1406
HaeII RGCGCY 2 cut(s) 449, 660
HaeIII GGCC 4 cut(s) 354, 1023, 1398, 1700
HapII CCGG 3 cut(s) 1158, 1187, 1484
HhaI GCGC 2 cut(s) 448, 659
Hin6I GCGC 2 cut(s) 446, 657
HinP1I GCGC 2 cut(s) 446, 657
HincII GTYRAC 1 cut(s) 587
HindII GTYRAC 1 cut(s) 587
HinfI GANTC 4 cut(s) 719, 1703, 1740, 1786
HpaII CCGG 3 cut(s) 1158, 1187, 1484
HphI GGTGA 4 cut(s) 1203, 1283, 1332, 1594
Hpy166II GTNNAC 5 cut(s) 587, 890, 946, 1387, 1585
Hpy188III TCNNGA 4 cut(s) 106, 185, 485, 1366
Hpy8I GTNNAC 5 cut(s) 587, 890, 946, 1387, 1585
HpyAV CCTTC 8 cut(s) 91, 185, 186, 842, 1135, 1641, 1714, 1786
HpyCH4III ACNGT 6 cut(s) 591, 602, 815, 1072, 1090, 1717
HpyCH4IV ACGT 1 cut(s) 1689
HpyF10VI GCNNNNNNNGC 7 cut(s) 377, 452, 461, 614, 623, 926, 1802
HpyF3I CTNAG 6 cut(s) 1295, 1377, 1450, 1662, 1777, 1872
HpySE526I ACGT 1 cut(s) 1689
HspAI GCGC 2 cut(s) 446, 657
KpnI GGTACC 1 cut(s) 1275
Ksp22I TGATCA 1 cut(s) 1897
Kzo9I GATC 6 cut(s) 169, 276, 895, 1480, 1877, 1897
LmnI GCTCC 5 cut(s) 926, 1114, 1151, 1732, 1914
Lsp1109I GCAGC 8 cut(s) 367, 514, 601, 904, 1003, 1217, 1319, 1333
LweI GCATC 4 cut(s) 514, 789, 1007, 1761
MabI ACCWGGT 1 cut(s) 471
MaeI CTAG 6 cut(s) 327, 372, 687, 1134, 1202, 1856
MaeII ACGT 1 cut(s) 1689
MaeIII GTNAC 9 cut(s) 133, 162, 230, 815, 825, 1090, 1338, 1681, 1807
MalI GATC 6 cut(s) 171, 278, 897, 1482, 1879, 1899
MboI GATC 6 cut(s) 169, 276, 895, 1480, 1877, 1897
MboII GAAGA 6 cut(s) 19, 26, 608, 648, 983, 1698
MflI RGATCY 1 cut(s) 1877
MhlI GDGCHC 3 cut(s) 334, 1656, 1919
MlyI GAGTC 1 cut(s) 728
MroXI GAANNNNTTC 2 cut(s) 18, 101
MseI TTAA 4 cut(s) 752, 789, 840, 1353
MslI CAYNNNNRTG 4 cut(s) 158, 563, 1296, 1942
MspA1I CMGCKG 1 cut(s) 1376
MspI CCGG 3 cut(s) 1158, 1187, 1484
MspR9I CCNGG 7 cut(s) 72, 473, 783, 1158, 1159, 1188, 1485
Mva1269I GAATGC 2 cut(s) 201, 1197
MvaI CCWGG 3 cut(s) 72, 473, 783
MwoI GCNNNNNNNGC 7 cut(s) 377, 452, 461, 614, 623, 926, 1802
NciI CCSGG 4 cut(s) 1158, 1159, 1188, 1485
NcoI CCATGG 2 cut(s) 355, 1113
NdeI CATATG 1 cut(s) 1151
NdeII GATC 6 cut(s) 169, 276, 895, 1480, 1877, 1897
NheI GCTAGC 1 cut(s) 371
NlaIV GGNNCC 5 cut(s) 403, 928, 1140, 1273, 1916
NmuCI GTSAC 3 cut(s) 230, 825, 1338
NspI RCATGY 1 cut(s) 1559
NspV TTCGAA 1 cut(s) 1244
OliI CACNNNNGTG 2 cut(s) 158, 1296
PagI TCATGA 1 cut(s) 1365
PaqCI CACCTGC 3 cut(s) 216, 911, 1351
PceI AGGCCT 1 cut(s) 1023
PciI ACATGT 1 cut(s) 1555
PctI GAATGC 2 cut(s) 201, 1197
PdmI GAANNNNTTC 2 cut(s) 18, 101
PfeI GAWTC 3 cut(s) 1703, 1740, 1786
PfoI TCCNGGA 1 cut(s) 70
PkrI GCNGC 9 cut(s) 382, 529, 616, 919, 931, 1018, 1207, 1309, 1348
PleI GAGTC 1 cut(s) 727
PpsI GAGTC 1 cut(s) 727
PscI ACATGT 1 cut(s) 1555
PshBI ATTAAT 2 cut(s) 789, 840
Psp1406I AACGTT 1 cut(s) 1689
Psp6I CCWGG 3 cut(s) 70, 471, 781
PspEI GGTNACC 1 cut(s) 1338
PspFI CCCAGC 1 cut(s) 383
PspGI CCWGG 3 cut(s) 70, 471, 781
PspN4I GGNNCC 5 cut(s) 403, 928, 1140, 1273, 1916
PspPI GGNCC 2 cut(s) 1139, 1184
PstI CTGCAG 3 cut(s) 532, 922, 1021
PstNI CAGNNNCTG 1 cut(s) 1715
PsuI RGATCY 1 cut(s) 1877
PvuII CAGCTG 1 cut(s) 1376
RsaI GTAC 2 cut(s) 502, 1273
RsaNI GTAC 2 cut(s) 501, 1272
RseI CAYNNNNRTG 4 cut(s) 158, 563, 1296, 1942
SaqAI TTAA 4 cut(s) 752, 789, 840, 1353
SatI GCNGC 9 cut(s) 381, 528, 615, 918, 930, 1017, 1206, 1308, 1347
Sau3AI GATC 6 cut(s) 169, 276, 895, 1480, 1877, 1897
Sau96I GGNCC 2 cut(s) 1139, 1184
ScaI AGTACT 1 cut(s) 502
SchI GAGTC 1 cut(s) 728
ScrFI CCNGG 7 cut(s) 72, 473, 783, 1158, 1159, 1188, 1485
SduI GDGCHC 3 cut(s) 334, 1656, 1919
SexAI ACCWGGT 1 cut(s) 471
SfaNI GCATC 4 cut(s) 514, 789, 1007, 1761
SfcI CTRYAG 5 cut(s) 528, 918, 1017, 1068, 1371
SfuI TTCGAA 1 cut(s) 1244
SinI GGWCC 2 cut(s) 1139, 1184
SmaI CCCGGG 1 cut(s) 1159
SmiMI CAYNNNNRTG 4 cut(s) 158, 563, 1296, 1942
SpeI ACTAGT 1 cut(s) 1855
SseBI AGGCCT 1 cut(s) 1023
SsiI CCGC 3 cut(s) 930, 1533, 1805
SspI AATATT 1 cut(s) 1904
SspMI CTAG 6 cut(s) 327, 372, 687, 1134, 1202, 1856
StuI AGGCCT 1 cut(s) 1023
StyD4I CCNGG 7 cut(s) 70, 471, 781, 1156, 1157, 1186, 1483
StyI CCWWGG 3 cut(s) 355, 1113, 1735
TaaI ACNGT 6 cut(s) 591, 602, 815, 1072, 1090, 1717
TaiI ACGT 1 cut(s) 1692
TaqI TCGA 5 cut(s) 497, 534, 546, 1238, 1244
TaqII GACCGA 1 cut(s) 572
TatI WGTACW 1 cut(s) 500
TauI GCSGC 1 cut(s) 932
TfiI GAWTC 3 cut(s) 1703, 1740, 1786
Tru1I TTAA 4 cut(s) 752, 789, 840, 1353
Tru9I TTAA 4 cut(s) 752, 789, 840, 1353
TscAI CASTG 9 cut(s) 160, 628, 834, 982, 1414, 1438, 1552, 1720, 1750
TseFI GTSAC 3 cut(s) 230, 825, 1338
TseI GCWGC 8 cut(s) 380, 527, 614, 917, 1016, 1205, 1307, 1346
Tsp45I GTSAC 3 cut(s) 230, 825, 1338
TspDTI ATGAA 6 cut(s) 20, 204, 504, 1428, 1623, 1799
TspMI CCCGGG 1 cut(s) 1157
TspRI CASTG 9 cut(s) 160, 628, 834, 982, 1414, 1438, 1552, 1720, 1750
VpaK11BI GGWCC 2 cut(s) 1139, 1184
VspI ATTAAT 2 cut(s) 789, 840
XapI RAATTY 7 cut(s) 43, 118, 388, 639, 707, 1076, 1240
XceI RCATGY 1 cut(s) 1559
XcmI CCANNNNNNNNNTGG 1 cut(s) 213
XmaI CCCGGG 1 cut(s) 1157
XmiI GTMKAC 1 cut(s) 889
XmnI GAANNNNTTC 2 cut(s) 18, 101
XspI CTAG 6 cut(s) 327, 372, 687, 1134, 1202, 1856
ZrmI AGTACT 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.