Prupe.5G086000_v2.0.a1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
N/A
Physical Location & Seq
Forward (+)
9763527 .. 9766488
2962 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G086000.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 1488 bp
ATGTGTGTTTTGCTGTCATTTGTGAAAGTTCTTATTCTTCTAATCATCCTTTCCTTCTCAGAAATCTGTTCAACACAGGTACTAGAATCCCCATCTCCAAGTACTTTAGCAACCTACATTGTTCACCTTAAAAAGCCACATGACACAGATTTTTCAGAATTGCAAGACTTACAAAGCTGGCACCAGTCATTTTTGCCCACGGAGACAGAAACCGTGAACCCGAATCCATACCTGGCAATCCAAAAGGGTATCCTCGAGAGCGCTTCGCCTGGGAATCTTGGTCCATTCAATGGTACAGTGCAAAATGCAGGCCCATGGATCCTTACTGTTGGAGCAAGCACCATTGATAGAAGTTTCAAAGCCCCTCTTGGTTTGTTGAATCCAACAACATTGCCACCTCTTGTATACCCCACCACAAGCAACCACACATTATCTGAATTCTGCAATCAGAGAACGTTGGCAAATGTTAGGGACATGGTGAAAGGAAAGGTAGTGCTATGTCAGGGACGTGGAGGGGAGGCCGGCATTGCTAGAGGGGCAGAAGTGAAGAAATATGGAGGGGTTGCCATGATTCTCATGAATGAAGAGCTTGATGGCTATACTATCTCGGCAGAAACTCATCAGTGGTTGCAAATCAAGGCCTATATAAATTCAACCTCAACACCAACTGCCACAATCTTATACAAAGGAACTCAAACAGGAAACTCATCTGCTCCCATTGTGCTTTCCTTTTCTTCAAGAGGTCCATATGCAGCAATCCCCGGGATTCTGAAACCGGACATTATTGGACCTGGAGGAAATATTCTTGGAGCCTGGCCGTCTGACTCATCATTTTCAGTGAGTTCAGCAGCGTTGCTCAAGAGCTCACACCCTGACTGGTCACCAGCTGCTATTAAGTCTGCAATGATGACAACTGCTGATGTACTAGACCATGAAGACAAGCCCATTCTTGACTATAATTATGCTCCAGCTGAACTCTTTGCCACTGGTGCAGGCCATGTTAACCCCTCCAAAGCAAACGATCCGGGGCTTGTCTACGACATCCAACCAGATGATTACATTCCTTATTTGTGCGGTTTCAAGTACAAAGACAAAGAGATAAAAAGCATACCTGAATCACAGCTGAACTACCCTTCATTTTCGATAACATTTGGATCAAGTCCGCAGGTGGTTACAAGGACAGTGACTAATGTTGGCTCAGCTTCATCAAATTACACTGTGAAAGGTTGTTTCTCCAGAGGCAGTAAGCATTCAATTCAAGTAAAGCCTGAGGAGATTGTGTTCACCAAGCTGAAACAGACAGCAACTTACAAAGTGACTTTCAGCCTAGATGATGAGGGCTATAGTCAGAGTGCTGGCCATAAGTTATTTGCTCGAGGCTCCCTGCAATGGATTTCTGCTGAACATGGTGTTAGTAGCCCAATTTCTGTCGCATTCAAAAACGACGATAGGATGCTGCTACTCACTGTAAGTTCTCTAAAGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

496

Amino Acids

53.41

Weight (kDa)

6.42

Isoelectric Point (pI)

33.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1156
Acc36I ACCTGC 1 cut(s) 1156
AccB1I GGYRCC 1 cut(s) 180
AccB7I CCANNNNNTGG 1 cut(s) 290
AccI GTMKAC 2 cut(s) 405, 1035
AciI CCGC 2 cut(s) 1074, 1163
AclI AACGTT 1 cut(s) 455
AclWI GGATC 4 cut(s) 313, 326, 1016, 1162
AcoI YGGCCR 2 cut(s) 815, 1357
AcsI RAATTY 2 cut(s) 437, 649
AfaI GTAC 5 cut(s) 81, 103, 295, 924, 1085
AfeI AGCGCT 1 cut(s) 262
AfiI CCNNNNNNNGG 2 cut(s) 290, 1389
AjiI CACGTC 1 cut(s) 509
AjnI CCWGG 4 cut(s) 231, 268, 790, 812
AloI GAACNNNNNNTCC 2 cut(s) 1265, 1297
AluBI AGCT 8 cut(s) 177, 589, 864, 887, 971, 1123, 1202, 1291
AluI AGCT 8 cut(s) 177, 589, 864, 887, 971, 1123, 1202, 1291
Alw21I GWGCWC 1 cut(s) 866
Alw26I GTCTC 1 cut(s) 197
AlwI GGATC 4 cut(s) 313, 326, 1016, 1162
Ama87I CYCGRG 3 cut(s) 254, 761, 1374
Aor51HI AGCGCT 1 cut(s) 262
AoxI GGCC 6 cut(s) 310, 519, 639, 815, 994, 1357
ApeKI GCWGC 4 cut(s) 752, 848, 887, 1456
ApoI RAATTY 2 cut(s) 437, 649
AspLEI GCGC 1 cut(s) 263
AspS9I GGNCC 4 cut(s) 281, 311, 743, 788
AsuC2I CCSGG 3 cut(s) 762, 763, 1026
AsuHPI GGTGA 4 cut(s) 116, 490, 873, 1276
AvaI CYCGRG 3 cut(s) 254, 761, 1374
AvaII GGWCC 3 cut(s) 281, 743, 788
AxyI CCTNAGG 1 cut(s) 1269
BalI TGGCCA 1 cut(s) 1359
BamHI GGATCC 1 cut(s) 318
BanI GGYRCC 1 cut(s) 180
BanII GRGCYC 1 cut(s) 866
BbsI GAAGAC 1 cut(s) 942
Bbv12I GWGCWC 1 cut(s) 866
BbvI GCAGC 4 cut(s) 764, 860, 874, 1443
BccI CCATC 2 cut(s) 100, 587
BceAI ACGGC 1 cut(s) 802
BciT130I CCWGG 4 cut(s) 233, 270, 792, 814
BciVI GTATCC 1 cut(s) 260
BcnI CCSGG 3 cut(s) 762, 763, 1026
BcoDI GTCTC 1 cut(s) 197
BfaI CTAG 4 cut(s) 83, 531, 926, 1328
BfmI CTRYAG 1 cut(s) 1342
BfoI RGCGCY 1 cut(s) 264
BfuAI ACCTGC 1 cut(s) 1156
BfuI GTATCC 1 cut(s) 260
BisI GCNGC 4 cut(s) 753, 849, 888, 1457
BlpI GCTNAGC 1 cut(s) 1198
BlsI GCNGC 4 cut(s) 754, 850, 889, 1458
BmcAI AGTACT 1 cut(s) 103
Bme1390I CCNGG 7 cut(s) 233, 270, 762, 763, 792, 814, 1026
Bme18I GGWCC 3 cut(s) 281, 743, 788
BmeT110I CYCGRG 3 cut(s) 254, 761, 1374
BmgBI CACGTC 1 cut(s) 509
BmgT120I GGNCC 4 cut(s) 281, 311, 743, 788
BmiI GGNNCC 4 cut(s) 182, 320, 811, 1381
BmrFI CCNGG 7 cut(s) 233, 270, 762, 763, 792, 814, 1026
BmsI GCATC 1 cut(s) 1443
BpiI GAAGAC 1 cut(s) 942
BpmI CTGGAG 3 cut(s) 813, 951, 1219
Bpu1102I GCTNAGC 1 cut(s) 1198
BpuEI CTTGAG 1 cut(s) 842
BpuMI CCSGG 3 cut(s) 762, 763, 1026
BsaJI CCNNGG 6 cut(s) 198, 269, 314, 760, 761, 1025
BsaWI WCCGGW 1 cut(s) 775
BsaXI ACNNNNNCTCC 2 cut(s) 1265, 1295
Bsc4I CCNNNNNNNGG 2 cut(s) 290, 1389
Bse118I RCCGGY 1 cut(s) 521
Bse1I ACTGG 3 cut(s) 184, 881, 991
Bse21I CCTNAGG 1 cut(s) 1269
Bse3DI GCAATG 4 cut(s) 389, 525, 909, 1394
BseBI CCWGG 4 cut(s) 233, 270, 792, 814
BseDI CCNNGG 6 cut(s) 198, 269, 314, 760, 761, 1025
BseGI GGATG 3 cut(s) 45, 1041, 1458
BseLI CCNNNNNNNGG 2 cut(s) 290, 1389
BseMI GCAATG 4 cut(s) 389, 525, 909, 1394
BseMII CTCAG 3 cut(s) 72, 1212, 1260
BseNI ACTGG 3 cut(s) 184, 881, 991
BseRI GAGGAG 1 cut(s) 1286
BseXI GCAGC 4 cut(s) 764, 860, 874, 1443
BsgI GTGCAG 1 cut(s) 1011
BshFI GGCC 6 cut(s) 312, 521, 641, 817, 996, 1359
BshNI GGYRCC 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 866
BsiHKCI CYCGRG 3 cut(s) 254, 761, 1374
BsiSI CCGG 4 cut(s) 522, 762, 776, 1025
BslFI GGGAC 2 cut(s) 485, 519
BslI CCNNNNNNNGG 2 cut(s) 290, 1389
BsmAI GTCTC 1 cut(s) 197
BsmFI GGGAC 2 cut(s) 485, 519
BsmI GAATGC 2 cut(s) 1249, 1433
BsnI GGCC 6 cut(s) 312, 521, 641, 817, 996, 1359
BsoBI CYCGRG 3 cut(s) 254, 761, 1374
Bsp1286I GDGCHC 1 cut(s) 866
Bsp143I GATC 3 cut(s) 318, 1021, 1154
Bsp1720I GCTNAGC 1 cut(s) 1198
Bsp19I CCATGG 1 cut(s) 314
BspACI CCGC 2 cut(s) 1074, 1163
BspANI GGCC 6 cut(s) 312, 521, 641, 817, 996, 1359
BspCNI CTCAG 3 cut(s) 71, 1211, 1261
BspHI TCATGA 1 cut(s) 576
BspLI GGNNCC 4 cut(s) 182, 320, 811, 1381
BspMI ACCTGC 1 cut(s) 1156
BspPI GGATC 4 cut(s) 313, 326, 1016, 1162
BspQI GCTCTTC 1 cut(s) 579
BspT107I GGYRCC 1 cut(s) 180
BsrDI GCAATG 4 cut(s) 389, 525, 909, 1394
BsrFI RCCGGY 1 cut(s) 521
BsrI ACTGG 3 cut(s) 184, 881, 991
BssAI RCCGGY 1 cut(s) 521
BssECI CCNNGG 6 cut(s) 198, 269, 314, 760, 761, 1025
BssMI GATC 3 cut(s) 318, 1021, 1154
BssNAI GTATAC 1 cut(s) 406
BssT1I CCWWGG 1 cut(s) 314
Bst1107I GTATAC 1 cut(s) 406
Bst2UI CCWGG 4 cut(s) 233, 270, 792, 814
Bst4CI ACNGT 6 cut(s) 214, 298, 328, 1183, 1219, 1468
Bst6I CTCTTC 1 cut(s) 579
BstC8I GCNNGC 6 cut(s) 179, 310, 337, 523, 994, 1357
BstDEI CTNAG 3 cut(s) 58, 1198, 1269
BstDSI CCRYGG 2 cut(s) 198, 314
BstEII GGTNACC 1 cut(s) 879
BstF5I GGATG 3 cut(s) 45, 1041, 1458
BstH2I RGCGCY 1 cut(s) 264
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 3 cut(s) 321, 1024, 1157
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 3 cut(s) 318, 1021, 1154
BstMWI GCNNNNNNNGC 3 cut(s) 527, 536, 989
BstNI CCWGG 4 cut(s) 233, 270, 792, 814
BstPI GGTNACC 1 cut(s) 879
BstSCI CCNGG 7 cut(s) 231, 268, 760, 761, 790, 812, 1024
BstSFI CTRYAG 1 cut(s) 1342
BstV1I GCAGC 4 cut(s) 764, 860, 874, 1443
BstV2I GAAGAC 1 cut(s) 942
BstX2I RGATCY 1 cut(s) 318
BstYI RGATCY 1 cut(s) 318
BstZ17I GTATAC 1 cut(s) 406
Bsu36I CCTNAGG 1 cut(s) 1269
BsuI GTATCC 1 cut(s) 260
BsuRI GGCC 6 cut(s) 312, 521, 641, 817, 996, 1359
BtgI CCRYGG 2 cut(s) 198, 314
BtrI CACGTC 1 cut(s) 509
BtsCI GGATG 3 cut(s) 45, 1041, 1458
BtsIMutI CAGTG 7 cut(s) 303, 629, 843, 984, 1188, 1215, 1464
BveI ACCTGC 1 cut(s) 1156
Cac8I GCNNGC 6 cut(s) 179, 310, 337, 523, 994, 1357
CciI TCATGA 1 cut(s) 576
CfoI GCGC 1 cut(s) 263
Cfr10I RCCGGY 1 cut(s) 521
Cfr13I GGNCC 4 cut(s) 281, 311, 743, 788
Cfr9I CCCGGG 1 cut(s) 761
Csp6I GTAC 5 cut(s) 80, 102, 294, 923, 1084
CviAII CATG 8 cut(s) 140, 315, 475, 568, 577, 932, 998, 1406
CviQI GTAC 5 cut(s) 80, 102, 294, 923, 1084
DdeI CTNAG 3 cut(s) 58, 1198, 1269
DpnI GATC 3 cut(s) 320, 1023, 1156
DpnII GATC 3 cut(s) 318, 1021, 1154
EaeI YGGCCR 2 cut(s) 815, 1357
Eam1104I CTCTTC 1 cut(s) 579
EarI CTCTTC 1 cut(s) 579
Ecl136II GAGCTC 1 cut(s) 864
Eco130I CCWWGG 1 cut(s) 314
Eco147I AGGCCT 1 cut(s) 641
Eco24I GRGCYC 1 cut(s) 866
Eco47I GGWCC 3 cut(s) 281, 743, 788
Eco47III AGCGCT 1 cut(s) 262
Eco53kI GAGCTC 1 cut(s) 864
Eco81I CCTNAGG 1 cut(s) 1269
Eco88I CYCGRG 3 cut(s) 254, 761, 1374
Eco91I GGTNACC 1 cut(s) 879
EcoICRI GAGCTC 1 cut(s) 864
EcoO65I GGTNACC 1 cut(s) 879
EcoRI GAATTC 1 cut(s) 437
EcoRII CCWGG 4 cut(s) 231, 268, 790, 812
EcoT14I CCWWGG 1 cut(s) 314
EcoT38I GRGCYC 1 cut(s) 866
ErhI CCWWGG 1 cut(s) 314
FaeI CATG 8 cut(s) 143, 318, 478, 571, 580, 935, 1001, 1409
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FaqI GGGAC 2 cut(s) 485, 519
FatI CATG 8 cut(s) 139, 314, 474, 567, 576, 931, 997, 1405
FauNDI CATATG 1 cut(s) 748
FblI GTMKAC 2 cut(s) 405, 1035
Fnu4HI GCNGC 4 cut(s) 753, 849, 888, 1457
FokI GGATG 3 cut(s) 32, 1028, 1465
FriOI GRGCYC 1 cut(s) 866
Fsp4HI GCNGC 4 cut(s) 753, 849, 888, 1457
FspBI CTAG 4 cut(s) 83, 531, 926, 1328
GlaI GCGC 1 cut(s) 262
GluI GCNGC 4 cut(s) 753, 849, 888, 1457
GsuI CTGGAG 3 cut(s) 813, 951, 1219
HaeII RGCGCY 1 cut(s) 264
HaeIII GGCC 6 cut(s) 312, 521, 641, 817, 996, 1359
HapII CCGG 4 cut(s) 522, 762, 776, 1025
HhaI GCGC 1 cut(s) 263
Hin1II CATG 8 cut(s) 143, 318, 478, 571, 580, 935, 1001, 1409
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HincII GTYRAC 1 cut(s) 1003
HindII GTYRAC 1 cut(s) 1003
HinfI GANTC 8 cut(s) 86, 223, 274, 379, 571, 766, 824, 1115
HpaI GTTAAC 1 cut(s) 1003
HpaII CCGG 4 cut(s) 522, 762, 776, 1025
HphI GGTGA 4 cut(s) 116, 490, 873, 1276
Hpy166II GTNNAC 6 cut(s) 124, 217, 406, 1003, 1036, 1284
Hpy188I TCNGA 7 cut(s) 61, 157, 436, 450, 771, 823, 1350
Hpy188III TCNNGA 6 cut(s) 256, 577, 738, 859, 950, 1236
Hpy8I GTNNAC 6 cut(s) 124, 217, 406, 1003, 1036, 1284
Hpy99I CGWCG 1 cut(s) 1448
HpyAV CCTTC 2 cut(s) 64, 1143
HpyCH4III ACNGT 6 cut(s) 214, 298, 328, 1183, 1219, 1468
HpyCH4IV ACGT 2 cut(s) 455, 508
HpyCH4V TGCA 9 cut(s) 163, 301, 308, 444, 631, 752, 902, 992, 1387
HpyF10VI GCNNNNNNNGC 3 cut(s) 527, 536, 989
HpyF3I CTNAG 3 cut(s) 58, 1198, 1269
HpySE526I ACGT 2 cut(s) 455, 508
Hsp92II CATG 8 cut(s) 143, 318, 478, 571, 580, 935, 1001, 1409
HspAI GCGC 1 cut(s) 261
KroI GCCGGC 1 cut(s) 521
KroNI GCCGGC 1 cut(s) 523
KspAI GTTAAC 1 cut(s) 1003
Kzo9I GATC 3 cut(s) 318, 1021, 1154
LguI GCTCTTC 1 cut(s) 579
LmnI GCTCC 5 cut(s) 332, 718, 809, 970, 1385
Lsp1109I GCAGC 4 cut(s) 764, 860, 874, 1443
LweI GCATC 1 cut(s) 1443
MaeI CTAG 4 cut(s) 83, 531, 926, 1328
MaeII ACGT 2 cut(s) 455, 508
MaeIII GTNAC 4 cut(s) 879, 1171, 1183, 1315
MalI GATC 3 cut(s) 320, 1023, 1156
MboI GATC 3 cut(s) 318, 1021, 1154
MboII GAAGA 5 cut(s) 29, 559, 596, 726, 947
MflI RGATCY 1 cut(s) 318
MhlI GDGCHC 1 cut(s) 866
MlsI TGGCCA 1 cut(s) 1359
MluCI AATT 7 cut(s) 158, 437, 649, 958, 1210, 1254, 1422
MluNI TGGCCA 1 cut(s) 1359
MlyI GAGTC 1 cut(s) 818
MmeI TCCRAC 3 cut(s) 310, 407, 1069
Mox20I TGGCCA 1 cut(s) 1359
MroNI GCCGGC 1 cut(s) 521
MscI TGGCCA 1 cut(s) 1359
MseI TTAA 3 cut(s) 129, 894, 1002
Msp20I TGGCCA 1 cut(s) 1359
MspA1I CMGCKG 3 cut(s) 887, 971, 1123
MspI CCGG 4 cut(s) 522, 762, 776, 1025
MspR9I CCNGG 7 cut(s) 233, 270, 762, 763, 792, 814, 1026
Mva1269I GAATGC 2 cut(s) 1249, 1433
MvaI CCWGG 4 cut(s) 233, 270, 792, 814
MwoI GCNNNNNNNGC 3 cut(s) 527, 536, 989
NaeI GCCGGC 1 cut(s) 523
NciI CCSGG 3 cut(s) 762, 763, 1026
NcoI CCATGG 1 cut(s) 314
NdeI CATATG 1 cut(s) 748
NdeII GATC 3 cut(s) 318, 1021, 1154
NgoMIV GCCGGC 1 cut(s) 521
NlaIII CATG 8 cut(s) 143, 318, 478, 571, 580, 935, 1001, 1409
NlaIV GGNNCC 4 cut(s) 182, 320, 811, 1381
NmeAIII GCCGAG 1 cut(s) 587
NmuCI GTSAC 3 cut(s) 879, 1183, 1315
PaeR7I CTCGAG 2 cut(s) 254, 1374
PagI TCATGA 1 cut(s) 576
PaqCI CACCTGC 1 cut(s) 1156
PceI AGGCCT 1 cut(s) 641
PciSI GCTCTTC 1 cut(s) 579
PctI GAATGC 2 cut(s) 1249, 1433
PdiI GCCGGC 1 cut(s) 523
PfeI GAWTC 7 cut(s) 86, 223, 274, 379, 571, 766, 1115
PflMI CCANNNNNTGG 1 cut(s) 290
PkrI GCNGC 4 cut(s) 754, 850, 889, 1458
PleI GAGTC 1 cut(s) 818
PpsI GAGTC 1 cut(s) 818
Psp124BI GAGCTC 1 cut(s) 866
Psp1406I AACGTT 1 cut(s) 455
Psp6I CCWGG 4 cut(s) 231, 268, 790, 812
PspEI GGTNACC 1 cut(s) 879
PspGI CCWGG 4 cut(s) 231, 268, 790, 812
PspN4I GGNNCC 4 cut(s) 182, 320, 811, 1381
PspPI GGNCC 4 cut(s) 281, 311, 743, 788
PspXI VCTCGAGB 1 cut(s) 1374
PsuI RGATCY 1 cut(s) 318
PvuII CAGCTG 3 cut(s) 887, 971, 1123
RsaI GTAC 5 cut(s) 81, 103, 295, 924, 1085
RsaNI GTAC 5 cut(s) 80, 102, 294, 923, 1084
SacI GAGCTC 1 cut(s) 866
SapI GCTCTTC 1 cut(s) 579
SaqAI TTAA 3 cut(s) 129, 894, 1002
SatI GCNGC 4 cut(s) 753, 849, 888, 1457
Sau3AI GATC 3 cut(s) 318, 1021, 1154
Sau96I GGNCC 4 cut(s) 281, 311, 743, 788
ScaI AGTACT 1 cut(s) 103
SchI GAGTC 1 cut(s) 818
ScrFI CCNGG 7 cut(s) 233, 270, 762, 763, 792, 814, 1026
SduI GDGCHC 1 cut(s) 866
SfaNI GCATC 1 cut(s) 1443
SfcI CTRYAG 1 cut(s) 1342
Sfr274I CTCGAG 2 cut(s) 254, 1374
SinI GGWCC 3 cut(s) 281, 743, 788
SlaI CTCGAG 2 cut(s) 254, 1374
SmaI CCCGGG 1 cut(s) 763
SmlI CTYRAG 3 cut(s) 254, 857, 1374
SmoI CTYRAG 3 cut(s) 254, 857, 1374
Sse9I AATT 7 cut(s) 158, 437, 649, 958, 1210, 1254, 1422
SseBI AGGCCT 1 cut(s) 641
SsiI CCGC 2 cut(s) 1074, 1163
SspI AATATT 1 cut(s) 802
SspMI CTAG 4 cut(s) 83, 531, 926, 1328
SstI GAGCTC 1 cut(s) 866
StuI AGGCCT 1 cut(s) 641
StyD4I CCNGG 7 cut(s) 231, 268, 760, 761, 790, 812, 1024
StyI CCWWGG 1 cut(s) 314
TaaI ACNGT 6 cut(s) 214, 298, 328, 1183, 1219, 1468
TaiI ACGT 2 cut(s) 458, 511
TaqI TCGA 3 cut(s) 255, 1142, 1375
TasI AATT 7 cut(s) 158, 437, 649, 958, 1210, 1254, 1422
TatI WGTACW 3 cut(s) 101, 922, 1083
TfiI GAWTC 7 cut(s) 86, 223, 274, 379, 571, 766, 1115
Tru1I TTAA 3 cut(s) 129, 894, 1002
Tru9I TTAA 3 cut(s) 129, 894, 1002
TscAI CASTG 7 cut(s) 303, 629, 843, 991, 1188, 1222, 1471
TseFI GTSAC 3 cut(s) 879, 1183, 1315
TseI GCWGC 4 cut(s) 752, 848, 887, 1456
Tsp45I GTSAC 3 cut(s) 879, 1183, 1315
TspDTI ATGAA 5 cut(s) 593, 597, 948, 1125, 1194
TspGWI ACGGA 1 cut(s) 215
TspMI CCCGGG 1 cut(s) 761
TspRI CASTG 7 cut(s) 303, 629, 843, 991, 1188, 1222, 1471
Van91I CCANNNNNTGG 1 cut(s) 290
VpaK11BI GGWCC 3 cut(s) 281, 743, 788
XapI RAATTY 2 cut(s) 437, 649
XhoI CTCGAG 2 cut(s) 254, 1374
XmaI CCCGGG 1 cut(s) 761
XmiI GTMKAC 2 cut(s) 405, 1035
XspI CTAG 4 cut(s) 83, 531, 926, 1328
ZrmI AGTACT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.