Prupe.5G100900_v2.0.a1

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
N/A
Physical Location & Seq
Reverse (-)
10876561 .. 10880599
4039 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G100900.1_v2.0.a1.CDS.3

Sequence Viewer

Length: 1284 bp
ATGTTGCTGGCAACATTTGCTTTACTTTTTAATGGAGATATTGCAGTTCAAGGATGGGGAAGGTTATCAAAGGATGAAGACTTGGAGCTGGACACACTGCTTAACCTTATCAATAAACTTCCAATCAAGAGTTTCAGGACTGGATATGGTAATATTGCAGATTGCATTGATATCTACAAACAACTTGCTTTCGATCATCCTCTGCTCAAGAATCATACAATCCAGGAACCTACATTGCCCACAAGGATCAGTGCCCATGAGAAGGCAACAAGGGAAGATCTAATAATGGCAAAAAATATGAAGTCTTTGGGACTAAACTACCCAATTACAAAAACTCACAGGAGCATCTCCAATAAAATTGACATAAAGGGCCATCATGTTAATCTAAATCTATATCCCTATGGCAGCCGACTTTATACTTTTTGGACTGCAGATGGTTACCAAAAGACTGGTTGTTCCAATATTTTATGTCCAGGATTTGTGCAAGTTAGCTCTGATATACCCCTTGGCCTCAAGCTACCAACTTCTATTTACAGGGGTAAACAATATGATATGCTGCTCAGTTTACATCAGGATCGTCATACAAAGAATTGGTGGCTCATATTTGAAGATGCATACGTTGGTTACTGGCCAAGAGAAATATTCACTAGTTTGGCACATGGAGCTTCTCTTATGTCATGGGAAGGAGAGATTTACAGCAAAATTAAAGTCGTTACTGATCGATCACAAGATTATGTTGATCCTGAAGATGATGATCTCAAAATGTACATTGATAAACCTTATTGCTATAATGGTAGACAGACTGAAAATGGCGATATTGTAGATTGCATTGATATCTACAAACAACTAGCTTTTGATCATCCTCTCTCTTCAAGAATCATACAATTCAGAAACCATAATCACGGAGAAAGAGGACGCGTCAATATATGGAACCCTAGTGTCCCCCCCACTTCCGAATTTCGAAACGATGATACACATCATTTGGCATATGGTTACCAAAAGACTGGTTGCTTCAATCTTCTGTGTCCCGGATTTATACAAGTTAGCTCCGGAGAGGTTTACATCCAACCAACAGACCTAAGCCCTGCCATGGGAAGTGGCCATTTTCCACAAGAGGGTTATGAGAAGTCTGCCTATATCACTCAGATCATAGTAGTCATCAATGAATCTGCAGGTTATGGTGATCCTCCAGTAGATCCTTGCCGTCTAAAAATCTCTGCTGCTAAACCCTATTGCTATAATGTCATACAGCACGTAGGGAAGGCACCCTTGAGAATAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

428

Amino Acids

48.57

Weight (kDa)

6.46

Isoelectric Point (pI)

26.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1163
AccB1I GGYRCC 1 cut(s) 1264
AccI GTMKAC 1 cut(s) 796
AccII CGCG 1 cut(s) 918
AccIII TCCGGA 1 cut(s) 1049
AclWI GGATC 5 cut(s) 254, 582, 734, 1178, 1190
AcoI YGGCCR 2 cut(s) 629, 1099
AcsI RAATTY 1 cut(s) 956
AcuI CTGAAG 1 cut(s) 765
AfaI GTAC 1 cut(s) 767
AfiI CCNNNNNNNGG 4 cut(s) 262, 1090, 1091, 1115
AflIII ACRYGT 1 cut(s) 916
AgsI TTSAA 4 cut(s) 50, 608, 873, 1015
AhlI ACTAGT 1 cut(s) 647
AjnI CCWGG 2 cut(s) 222, 472
AluBI AGCT 6 cut(s) 88, 492, 517, 665, 851, 1047
AluI AGCT 6 cut(s) 88, 492, 517, 665, 851, 1047
AlwI GGATC 5 cut(s) 254, 582, 734, 1178, 1190
Aor13HI TCCGGA 1 cut(s) 1049
AoxI GGCC 4 cut(s) 370, 508, 629, 1099
ApeKI GCWGC 3 cut(s) 405, 556, 1220
ApoI RAATTY 1 cut(s) 956
ArsI GACNNNNNNTTYG 2 cut(s) 693, 725
AspS9I GGNCC 1 cut(s) 370
AsuC2I CCSGG 1 cut(s) 1029
AsuHPI GGTGA 1 cut(s) 1193
AsuII TTCGAA 1 cut(s) 961
BaeGI GKGCMC 1 cut(s) 256
BalI TGGCCA 2 cut(s) 631, 1101
BanI GGYRCC 1 cut(s) 1264
BbsI GAAGAC 1 cut(s) 84
BbvI GCAGC 3 cut(s) 417, 543, 1207
BccI CCATC 3 cut(s) 48, 381, 428
BceAI ACGGC 1 cut(s) 1188
BciT130I CCWGG 2 cut(s) 224, 474
BclI TGATCA 1 cut(s) 856
BcnI CCSGG 1 cut(s) 1029
BcuI ACTAGT 1 cut(s) 647
BfaI CTAG 3 cut(s) 648, 848, 936
BfmI CTRYAG 2 cut(s) 429, 1170
BfuAI ACCTGC 1 cut(s) 1163
BglII AGATCT 1 cut(s) 277
BisI GCNGC 3 cut(s) 406, 557, 1221
BlsI GCNGC 3 cut(s) 407, 558, 1222
Bme1390I CCNGG 3 cut(s) 224, 474, 1029
BmgT120I GGNCC 1 cut(s) 370
BmiI GGNNCC 3 cut(s) 228, 932, 1266
BmrFI CCNGG 3 cut(s) 224, 474, 1029
BmsI GCATC 2 cut(s) 354, 601
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 1173
Bpu10I CCTNAGC 1 cut(s) 1079
Bpu14I TTCGAA 1 cut(s) 961
BpuEI CTTGAG 2 cut(s) 191, 497
BpuMI CCSGG 1 cut(s) 1029
Bsa29I ATCGAT 1 cut(s) 721
BsaAI YACGTR 1 cut(s) 1255
BsaBI GATNNNNATC 2 cut(s) 753, 975
BsaJI CCNNGG 2 cut(s) 505, 1089
BsaWI WCCGGW 1 cut(s) 1049
Bsc4I CCNNNNNNNGG 4 cut(s) 262, 1090, 1091, 1115
Bse1I ACTGG 5 cut(s) 145, 454, 632, 1009, 1190
Bse3DI GCAATG 1 cut(s) 233
Bse8I GATNNNNATC 2 cut(s) 753, 975
BseAI TCCGGA 1 cut(s) 1049
BseBI CCWGG 2 cut(s) 224, 474
BseCI ATCGAT 1 cut(s) 721
BseDI CCNNGG 2 cut(s) 505, 1089
BseGI GGATG 5 cut(s) 59, 79, 196, 859, 1062
BseJI GATNNNNATC 2 cut(s) 753, 975
BseLI CCNNNNNNNGG 4 cut(s) 262, 1090, 1091, 1115
BseMI GCAATG 1 cut(s) 233
BseMII CTCAG 2 cut(s) 574, 1157
BseNI ACTGG 5 cut(s) 145, 454, 632, 1009, 1190
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 3 cut(s) 417, 543, 1207
Bsh1236I CGCG 1 cut(s) 918
BshFI GGCC 4 cut(s) 372, 510, 631, 1101
BshNI GGYRCC 1 cut(s) 1264
BshVI ATCGAT 1 cut(s) 721
BsiSI CCGG 2 cut(s) 1029, 1050
BslFI GGGAC 3 cut(s) 324, 926, 1011
BslI CCNNNNNNNGG 4 cut(s) 262, 1090, 1091, 1115
BsmFI GGGAC 3 cut(s) 324, 926, 1011
BsnI GGCC 4 cut(s) 372, 510, 631, 1101
Bsp119I TTCGAA 1 cut(s) 961
Bsp1286I GDGCHC 1 cut(s) 256
Bsp13I TCCGGA 1 cut(s) 1049
Bsp1407I TGTACA 1 cut(s) 765
Bsp19I CCATGG 1 cut(s) 1089
BspANI GGCC 4 cut(s) 372, 510, 631, 1101
BspCNI CTCAG 2 cut(s) 573, 1156
BspDI ATCGAT 1 cut(s) 721
BspEI TCCGGA 1 cut(s) 1049
BspFNI CGCG 1 cut(s) 918
BspLI GGNNCC 3 cut(s) 228, 932, 1266
BspMAI CTGCAG 2 cut(s) 433, 1174
BspMI ACCTGC 1 cut(s) 1163
BspPI GGATC 5 cut(s) 254, 582, 734, 1178, 1190
BspT104I TTCGAA 1 cut(s) 961
BspT107I GGYRCC 1 cut(s) 1264
BsrDI GCAATG 1 cut(s) 233
BsrGI TGTACA 1 cut(s) 765
BsrI ACTGG 5 cut(s) 145, 454, 632, 1009, 1190
BssECI CCNNGG 2 cut(s) 505, 1089
BssT1I CCWWGG 2 cut(s) 505, 1089
Bst2UI CCWGG 2 cut(s) 224, 474
Bst6I CTCTTC 1 cut(s) 874
BstAPI GCANNNNNTGC 1 cut(s) 17
BstAUI TGTACA 1 cut(s) 765
BstBAI YACGTR 1 cut(s) 1255
BstBI TTCGAA 1 cut(s) 961
BstC8I GCNNGC 1 cut(s) 9
BstDEI CTNAG 3 cut(s) 560, 1079, 1143
BstDSI CCRYGG 1 cut(s) 1089
BstEII GGTNACC 2 cut(s) 437, 992
BstF5I GGATG 5 cut(s) 59, 79, 196, 859, 1062
BstFNI CGCG 1 cut(s) 918
BstMWI GCNNNNNNNGC 2 cut(s) 17, 662
BstNI CCWGG 2 cut(s) 224, 474
BstPI GGTNACC 2 cut(s) 437, 992
BstSCI CCNGG 3 cut(s) 222, 472, 1027
BstSFI CTRYAG 2 cut(s) 429, 1170
BstSLI GKGCMC 1 cut(s) 256
BstUI CGCG 1 cut(s) 918
BstV1I GCAGC 3 cut(s) 417, 543, 1207
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 2 cut(s) 277, 1195
BstXI CCANNNNNNTGG 2 cut(s) 449, 1004
BstYI RGATCY 2 cut(s) 277, 1195
Bsu15I ATCGAT 1 cut(s) 721
BsuRI GGCC 4 cut(s) 372, 510, 631, 1101
BsuTUI ATCGAT 1 cut(s) 721
BtgI CCRYGG 1 cut(s) 1089
BtsCI GGATG 5 cut(s) 59, 79, 196, 859, 1062
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 2 cut(s) 95, 256
BveI ACCTGC 1 cut(s) 1163
Cac8I GCNNGC 1 cut(s) 9
Cfr13I GGNCC 1 cut(s) 370
ClaI ATCGAT 1 cut(s) 721
CseI GACGC 2 cut(s) 907, 924
Csp6I GTAC 1 cut(s) 766
CviAII CATG 5 cut(s) 257, 377, 659, 678, 1090
CviQI GTAC 1 cut(s) 766
DdeI CTNAG 3 cut(s) 560, 1079, 1143
EaeI YGGCCR 2 cut(s) 629, 1099
Eam1104I CTCTTC 1 cut(s) 874
EarI CTCTTC 1 cut(s) 874
Eco130I CCWWGG 2 cut(s) 505, 1089
Eco32I GATATC 2 cut(s) 172, 835
Eco57I CTGAAG 1 cut(s) 765
Eco91I GGTNACC 2 cut(s) 437, 992
EcoO65I GGTNACC 2 cut(s) 437, 992
EcoRII CCWGG 2 cut(s) 222, 472
EcoRV GATATC 2 cut(s) 172, 835
EcoT14I CCWWGG 2 cut(s) 505, 1089
EcoT22I ATGCAT 1 cut(s) 616
ErhI CCWWGG 2 cut(s) 505, 1089
FaeI CATG 5 cut(s) 260, 380, 662, 681, 1093
FalI AAGNNNNNCTT 1 cut(s) 1253
FaqI GGGAC 3 cut(s) 324, 926, 1011
FatI CATG 5 cut(s) 256, 376, 658, 677, 1089
FauNDI CATATG 1 cut(s) 988
FbaI TGATCA 1 cut(s) 856
FblI GTMKAC 1 cut(s) 796
Fnu4HI GCNGC 3 cut(s) 406, 557, 1221
FokI GGATG 5 cut(s) 66, 86, 183, 846, 1049
Fsp4HI GCNGC 3 cut(s) 406, 557, 1221
FspBI CTAG 3 cut(s) 648, 848, 936
GluI GCNGC 3 cut(s) 406, 557, 1221
GsuI CTGGAG 1 cut(s) 1173
HaeIII GGCC 4 cut(s) 372, 510, 631, 1101
HapII CCGG 2 cut(s) 1029, 1050
HgaI GACGC 2 cut(s) 907, 924
Hin1II CATG 5 cut(s) 260, 380, 662, 681, 1093
HinfI GANTC 3 cut(s) 211, 876, 1166
HpaII CCGG 2 cut(s) 1029, 1050
HphI GGTGA 1 cut(s) 1193
Hpy166II GTNNAC 4 cut(s) 542, 566, 797, 1060
Hpy188I TCNGA 4 cut(s) 496, 890, 955, 1146
Hpy188III TCNNGA 7 cut(s) 127, 136, 208, 572, 743, 873, 1050
Hpy8I GTNNAC 4 cut(s) 542, 566, 797, 1060
HpyAV CCTTC 4 cut(s) 54, 256, 677, 1255
HpyCH4IV ACGT 2 cut(s) 618, 1254
HpyCH4V TGCA 8 cut(s) 44, 158, 165, 431, 484, 614, 828, 1172
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 662
HpyF3I CTNAG 3 cut(s) 560, 1079, 1143
HpySE526I ACGT 2 cut(s) 618, 1254
Hsp92II CATG 5 cut(s) 260, 380, 662, 681, 1093
Kpn2I TCCGGA 1 cut(s) 1049
Ksp22I TGATCA 1 cut(s) 856
LmnI GCTCC 4 cut(s) 85, 342, 662, 1052
Lsp1109I GCAGC 3 cut(s) 417, 543, 1207
LweI GCATC 2 cut(s) 354, 601
MaeI CTAG 3 cut(s) 648, 848, 936
MaeII ACGT 2 cut(s) 618, 1254
MaeIII GTNAC 4 cut(s) 437, 623, 712, 992
MboII GAAGA 6 cut(s) 89, 287, 620, 758, 861, 1010
MflI RGATCY 2 cut(s) 277, 1195
MhlI GDGCHC 1 cut(s) 256
MlsI TGGCCA 2 cut(s) 631, 1101
MluCI AATT 7 cut(s) 324, 357, 589, 702, 884, 956, 1278
MluI ACGCGT 1 cut(s) 916
MluNI TGGCCA 2 cut(s) 631, 1101
MmeI TCCRAC 1 cut(s) 1090
MnlI CCTC 7 cut(s) 210, 521, 873, 905, 1048, 1108, 1197
Mox20I TGGCCA 2 cut(s) 631, 1101
Mph1103I ATGCAT 1 cut(s) 616
MroI TCCGGA 1 cut(s) 1049
MscI TGGCCA 2 cut(s) 631, 1101
MseI TTAA 4 cut(s) 30, 102, 381, 705
Msp20I TGGCCA 2 cut(s) 631, 1101
MspI CCGG 2 cut(s) 1029, 1050
MspR9I CCNGG 3 cut(s) 224, 474, 1029
MvaI CCWGG 2 cut(s) 224, 474
MvnI CGCG 1 cut(s) 918
MwoI GCNNNNNNNGC 2 cut(s) 17, 662
NciI CCSGG 1 cut(s) 1029
NcoI CCATGG 1 cut(s) 1089
NdeI CATATG 1 cut(s) 988
NlaIII CATG 5 cut(s) 260, 380, 662, 681, 1093
NlaIV GGNNCC 3 cut(s) 228, 932, 1266
NsiI ATGCAT 1 cut(s) 616
NspV TTCGAA 1 cut(s) 961
PfeI GAWTC 3 cut(s) 211, 876, 1166
PfoI TCCNGGA 3 cut(s) 222, 472, 1027
PkrI GCNGC 3 cut(s) 407, 558, 1222
Ppu21I YACGTR 1 cut(s) 1255
Psp6I CCWGG 2 cut(s) 222, 472
PspEI GGTNACC 2 cut(s) 437, 992
PspGI CCWGG 2 cut(s) 222, 472
PspN4I GGNNCC 3 cut(s) 228, 932, 1266
PspPI GGNCC 1 cut(s) 370
PstI CTGCAG 2 cut(s) 433, 1174
PsuI RGATCY 2 cut(s) 277, 1195
RsaI GTAC 1 cut(s) 767
RsaNI GTAC 1 cut(s) 766
SaqAI TTAA 4 cut(s) 30, 102, 381, 705
SatI GCNGC 3 cut(s) 406, 557, 1221
Sau96I GGNCC 1 cut(s) 370
ScrFI CCNGG 3 cut(s) 224, 474, 1029
SduI GDGCHC 1 cut(s) 256
SfaNI GCATC 2 cut(s) 354, 601
SfcI CTRYAG 2 cut(s) 429, 1170
SfuI TTCGAA 1 cut(s) 961
SmlI CTYRAG 3 cut(s) 206, 512, 1270
SmoI CTYRAG 3 cut(s) 206, 512, 1270
SpeI ACTAGT 1 cut(s) 647
Sse9I AATT 7 cut(s) 324, 357, 589, 702, 884, 956, 1278
SspI AATATT 3 cut(s) 154, 463, 642
SspMI CTAG 3 cut(s) 648, 848, 936
StyD4I CCNGG 3 cut(s) 222, 472, 1027
StyI CCWWGG 2 cut(s) 505, 1089
TaiI ACGT 2 cut(s) 621, 1257
TaqI TCGA 3 cut(s) 192, 721, 961
TasI AATT 7 cut(s) 324, 357, 589, 702, 884, 956, 1278
TatI WGTACW 1 cut(s) 765
TfiI GAWTC 3 cut(s) 211, 876, 1166
Tru1I TTAA 4 cut(s) 30, 102, 381, 705
Tru9I TTAA 4 cut(s) 30, 102, 381, 705
TscAI CASTG 2 cut(s) 102, 256
TseI GCWGC 3 cut(s) 405, 556, 1220
TspDTI ATGAA 3 cut(s) 90, 314, 1179
TspGWI ACGGA 1 cut(s) 918
TspRI CASTG 2 cut(s) 102, 256
XapI RAATTY 1 cut(s) 956
XmiI GTMKAC 1 cut(s) 796
XspI CTAG 3 cut(s) 648, 848, 936
Zsp2I ATGCAT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.