Prupe.5G129400_v2.0.a1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
N/A
Physical Location & Seq
Forward (+)
12533445 .. 12534250
806 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G129400.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 606 bp
ATGGCAGTGCAAGTCGAAGATTTTGAAGTCCATCACGAGAAGGATAGCATGGATTTTTACGTGTTACTCCTTGAACCACACTCGCTAGGACGTGCCTACCAACCTTCTTCATCATCGCCTGCGGCGCCTCAATTTCTGATAAAGTTTGATTATCATATCAATCATGTCTACTATGATATTGGTTCCTCATATGATATAGGAACTCGTCGTAACACCACCCTCGTCCACCAACATCAAGAAAAACATGAAAAAATAGTTGAACTTGAGAAGCCTGTGTTGAGGAAATGCGGCAAGTCGTACGAGTTTCTTTCTAAGGTGTTGTCGACGATTGGAATCAAAAGAGGATCGTATGATAATGGCGGACTGCATGATGAAATAATGAGTGGGATTATAAAGTGGGGTGCCAAATTTCCACCGCCAAAGTTGTCCAAGGAGAGTCCAGAGCTGTGGGGGCTGTATGTTTATATTGGAAAAGACCATGACATGTATCGGTGTGATGAGTGTTTAATGGCTAGGGTTACGAGGAAATCAATGGCGAGCAAGGGGATGGTGTCGATCAAGAAGGTAGTAAAAAATGTCTCCATTCCCCGATATCGATTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

202

Amino Acids

23.17

Weight (kDa)

8.95

Isoelectric Point (pI)

44.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 392
AccB1I GGYRCC 2 cut(s) 124, 401
AccI GTMKAC 2 cut(s) 168, 323
AciI CCGC 4 cut(s) 122, 288, 360, 416
AclWI GGATC 1 cut(s) 352
AcsI RAATTY 1 cut(s) 407
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 1 cut(s) 299
AflIII ACRYGT 2 cut(s) 60, 483
AgsI TTSAA 3 cut(s) 26, 74, 260
AjiI CACGTC 1 cut(s) 92
AluBI AGCT 1 cut(s) 445
AluI AGCT 1 cut(s) 445
Alw26I GTCTC 1 cut(s) 583
AlwI GGATC 1 cut(s) 352
ApoI RAATTY 1 cut(s) 407
AspLEI GCGC 1 cut(s) 127
BanI GGYRCC 2 cut(s) 124, 401
BauI CACGAG 1 cut(s) 35
BccI CCATC 2 cut(s) 39, 541
BcoDI GTCTC 1 cut(s) 583
BfaI CTAG 2 cut(s) 86, 513
BfoI RGCGCY 1 cut(s) 128
BisI GCNGC 2 cut(s) 123, 289
BlsI GCNGC 2 cut(s) 124, 290
BmgBI CACGTC 1 cut(s) 92
BmiI GGNNCC 3 cut(s) 126, 184, 403
BpuEI CTTGAG 1 cut(s) 284
Bsa29I ATCGAT 1 cut(s) 595
BsaAI YACGTR 1 cut(s) 61
BsaBI GATNNNNATC 1 cut(s) 332
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 1 cut(s) 429
Bse8I GATNNNNATC 1 cut(s) 332
BseCI ATCGAT 1 cut(s) 595
BseDI CCNNGG 1 cut(s) 429
BseGI GGATG 1 cut(s) 552
BseJI GATNNNNATC 1 cut(s) 332
BshNI GGYRCC 2 cut(s) 124, 401
BshVI ATCGAT 1 cut(s) 595
BsiWI CGTACG 1 cut(s) 297
BsmAI GTCTC 1 cut(s) 583
Bsp143I GATC 2 cut(s) 344, 555
BspACI CCGC 4 cut(s) 122, 288, 360, 416
BspDI ATCGAT 1 cut(s) 595
BspLI GGNNCC 3 cut(s) 126, 184, 403
BspPI GGATC 1 cut(s) 352
BspT107I GGYRCC 2 cut(s) 124, 401
BssECI CCNNGG 1 cut(s) 429
BssMI GATC 2 cut(s) 344, 555
BssNI GRCGYC 1 cut(s) 125
BssSI CACGAG 1 cut(s) 35
BssT1I CCWWGG 1 cut(s) 429
Bst2BI CACGAG 1 cut(s) 35
BstACI GRCGYC 1 cut(s) 125
BstBAI YACGTR 1 cut(s) 61
BstC8I GCNNGC 2 cut(s) 120, 538
BstDEI CTNAG 1 cut(s) 312
BstF5I GGATG 1 cut(s) 552
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 1 cut(s) 127
BstKTI GATC 2 cut(s) 347, 558
BstMAI GTCTC 1 cut(s) 583
BstMBI GATC 2 cut(s) 344, 555
BstMWI GCNNNNNNNGC 2 cut(s) 124, 451
BstNSI RCATGY 1 cut(s) 487
BstXI CCANNNNNNTGG 1 cut(s) 447
Bsu15I ATCGAT 1 cut(s) 595
BsuTUI ATCGAT 1 cut(s) 595
BtgZI GCGATG 1 cut(s) 99
BtrI CACGTC 1 cut(s) 92
BtsCI GGATG 1 cut(s) 552
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 1 cut(s) 12
Cac8I GCNNGC 2 cut(s) 120, 538
CfoI GCGC 1 cut(s) 127
ClaI ATCGAT 1 cut(s) 595
Csp6I GTAC 1 cut(s) 298
CviAII CATG 6 cut(s) 49, 164, 245, 368, 479, 484
CviJI RGCY 4 cut(s) 271, 445, 454, 512
CviKI_1 RGCY 4 cut(s) 271, 445, 454, 512
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 1 cut(s) 312
DinI GGCGCC 1 cut(s) 126
DpnI GATC 2 cut(s) 346, 557
DpnII GATC 2 cut(s) 344, 555
EciI GGCGGA 1 cut(s) 375
Eco130I CCWWGG 1 cut(s) 429
Eco32I GATATC 1 cut(s) 593
EcoRV GATATC 1 cut(s) 593
EcoT14I CCWWGG 1 cut(s) 429
EgeI GGCGCC 1 cut(s) 126
EheI GGCGCC 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 429
FaeI CATG 6 cut(s) 52, 167, 248, 371, 482, 487
FatI CATG 6 cut(s) 48, 163, 244, 367, 478, 483
FauNDI CATATG 1 cut(s) 190
FblI GTMKAC 2 cut(s) 168, 323
Fnu4HI GCNGC 2 cut(s) 123, 289
FokI GGATG 1 cut(s) 559
Fsp4HI GCNGC 2 cut(s) 123, 289
FspBI CTAG 2 cut(s) 86, 513
GlaI GCGC 1 cut(s) 126
GluI GCNGC 2 cut(s) 123, 289
HaeII RGCGCY 1 cut(s) 128
HhaI GCGC 1 cut(s) 127
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 6 cut(s) 52, 167, 248, 371, 482, 487
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HincII GTYRAC 1 cut(s) 324
HindII GTYRAC 1 cut(s) 324
HinfI GANTC 2 cut(s) 333, 436
Hpy166II GTNNAC 3 cut(s) 169, 226, 324
Hpy188I TCNGA 1 cut(s) 138
Hpy188III TCNNGA 4 cut(s) 35, 236, 440, 559
Hpy8I GTNNAC 3 cut(s) 169, 226, 324
Hpy99I CGWCG 2 cut(s) 210, 328
HpyAV CCTTC 3 cut(s) 34, 114, 556
HpyCH4IV ACGT 2 cut(s) 60, 91
HpyCH4V TGCA 2 cut(s) 10, 367
HpyF10VI GCNNNNNNNGC 2 cut(s) 124, 451
HpyF3I CTNAG 1 cut(s) 312
HpySE526I ACGT 2 cut(s) 60, 91
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 6 cut(s) 52, 167, 248, 371, 482, 487
HspAI GCGC 1 cut(s) 125
KasI GGCGCC 1 cut(s) 124
Kzo9I GATC 2 cut(s) 344, 555
LpnPI CCDG 3 cut(s) 132, 285, 453
MaeI CTAG 2 cut(s) 86, 513
MaeII ACGT 2 cut(s) 60, 91
MaeIII GTNAC 3 cut(s) 63, 209, 517
MalI GATC 2 cut(s) 346, 557
MboI GATC 2 cut(s) 344, 555
MboII GAAGA 2 cut(s) 29, 99
MluCI AATT 2 cut(s) 131, 407
Mly113I GGCGCC 1 cut(s) 125
MlyI GAGTC 1 cut(s) 445
MnlI CCTC 6 cut(s) 138, 196, 230, 273, 335, 516
MseI TTAA 1 cut(s) 506
MwoI GCNNNNNNNGC 2 cut(s) 124, 451
NarI GGCGCC 1 cut(s) 125
NdeI CATATG 1 cut(s) 190
NdeII GATC 2 cut(s) 344, 555
NlaIII CATG 6 cut(s) 52, 167, 248, 371, 482, 487
NlaIV GGNNCC 3 cut(s) 126, 184, 403
NspI RCATGY 1 cut(s) 487
PciI ACATGT 1 cut(s) 483
PfeI GAWTC 1 cut(s) 333
Pfl23II CGTACG 1 cut(s) 297
PkrI GCNGC 2 cut(s) 124, 290
PleI GAGTC 1 cut(s) 444
PluTI GGCGCC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 444
Ppu21I YACGTR 1 cut(s) 61
PscI ACATGT 1 cut(s) 483
PsiI TTATAA 1 cut(s) 392
PspLI CGTACG 1 cut(s) 297
PspN4I GGNNCC 3 cut(s) 126, 184, 403
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
SalI GTCGAC 1 cut(s) 322
SaqAI TTAA 1 cut(s) 506
SatI GCNGC 2 cut(s) 123, 289
Sau3AI GATC 2 cut(s) 344, 555
SchI GAGTC 1 cut(s) 445
SetI ASST 6 cut(s) 63, 94, 106, 318, 447, 567
SfoI GGCGCC 1 cut(s) 126
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 2 cut(s) 131, 407
SsiI CCGC 4 cut(s) 122, 288, 360, 416
SspDI GGCGCC 1 cut(s) 124
SspMI CTAG 2 cut(s) 86, 513
StyI CCWWGG 1 cut(s) 429
TaiI ACGT 2 cut(s) 63, 94
TaqI TCGA 4 cut(s) 15, 323, 554, 595
TasI AATT 2 cut(s) 131, 407
TauI GCSGC 2 cut(s) 125, 291
TfiI GAWTC 1 cut(s) 333
Tru1I TTAA 1 cut(s) 506
Tru9I TTAA 1 cut(s) 506
TscAI CASTG 1 cut(s) 12
TspDTI ATGAA 3 cut(s) 99, 261, 387
TspRI CASTG 1 cut(s) 12
XapI RAATTY 1 cut(s) 407
XceI RCATGY 1 cut(s) 487
XmiI GTMKAC 2 cut(s) 168, 323
XspI CTAG 2 cut(s) 86, 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.