Prupe.5G141700_v2.0.a1

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
N/A
Physical Location & Seq
Forward (+)
13157696 .. 13158433
738 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G141700.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 627 bp
ATGTCTGCAAAAGATGACAGCTTTGGCAGCAGCTACCTCCCTTCTTTGGACGAAACAGACGATCTCAAAATTACCAAGCCAGACGTAGTGAGATTTGCCCTGATCATAATATTTGTTTGCGGAATGATATTTAGCGACATTACAGAGATTCCCACCGTCCCGAAATTCTCCGTCGACAATGCTTCCCTTACCCAATTCAACTACACCAAAACCAACCACTCTCTCTCCTACAACCTTGCCCTCAACATCACCATCACAAACTCCAAGAAGTACATCGGCCTCGAGTACAGTGACATCCAAGTCACTGCATATTATGGAAAGACAAGGTTTGCTTTTGTGACTTTGGTGAACGAAACGGCATCGTTTCACCAAAACCCTAATACCACAGTCAGCTTTGAGAATGTGGCTGTTCAAGGGCAGAAGTTGGTGCTGTTTGCGGAATCAAATTTTAACAAGTATAGTTTGGGGACCGCTGCCGGCGTTTACAGTGTTGACCTTTTGATTGCTTTCAAGGAAACGTTCCTCTCCGGCAATGCCATCTGCAATCTAAAGCTTCCTTTGGGTTTTAATGGAACAGCCGCGCCTGGTTTCCAGGCTACAAAGTGCTGGAGGAGACGTTATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

209

Amino Acids

23.12

Weight (kDa)

7.65

Isoelectric Point (pI)

19.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 174
AccII CGCG 1 cut(s) 581
AciI CCGC 4 cut(s) 120, 437, 471, 579
AclI AACGTT 1 cut(s) 518
AcsI RAATTY 2 cut(s) 164, 445
AfaI GTAC 2 cut(s) 272, 287
AfiI CCNNNNNNNGG 1 cut(s) 46
AgsI TTSAA 3 cut(s) 199, 413, 511
AjnI CCWGG 2 cut(s) 583, 591
AluBI AGCT 4 cut(s) 21, 33, 393, 553
AluI AGCT 4 cut(s) 21, 33, 393, 553
Alw26I GTCTC 1 cut(s) 607
Ama87I CYCGRG 1 cut(s) 281
AoxI GGCC 1 cut(s) 277
ApeKI GCWGC 3 cut(s) 27, 30, 473
ApoI RAATTY 2 cut(s) 164, 445
AspLEI GCGC 1 cut(s) 583
AspS9I GGNCC 1 cut(s) 468
AsuHPI GGTGA 3 cut(s) 241, 358, 359
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 1 cut(s) 468
BbvI GCAGC 3 cut(s) 39, 42, 460
BccI CCATC 2 cut(s) 260, 545
BceAI ACGGC 1 cut(s) 372
BciT130I CCWGG 2 cut(s) 585, 593
BclI TGATCA 1 cut(s) 102
BcoDI GTCTC 1 cut(s) 607
BisI GCNGC 4 cut(s) 28, 31, 474, 579
BlsI GCNGC 4 cut(s) 29, 32, 475, 580
Bme1390I CCNGG 2 cut(s) 585, 593
Bme18I GGWCC 1 cut(s) 468
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 468
BmiI GGNNCC 1 cut(s) 469
BmrFI CCNGG 2 cut(s) 585, 593
BmsI GCATC 1 cut(s) 368
BsaXI ACNNNNNCTCC 3 cut(s) 209, 239, 601
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse118I RCCGGY 1 cut(s) 476
Bse3DI GCAATG 1 cut(s) 538
BseBI CCWGG 2 cut(s) 585, 593
BseGI GGATG 1 cut(s) 294
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMI GCAATG 1 cut(s) 538
BseRI GAGGAG 1 cut(s) 625
BseXI GCAGC 3 cut(s) 39, 42, 460
Bsh1236I CGCG 1 cut(s) 581
BshFI GGCC 1 cut(s) 279
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 2 cut(s) 477, 528
BslFI GGGAC 2 cut(s) 143, 481
BslI CCNNNNNNNGG 1 cut(s) 46
BsmAI GTCTC 1 cut(s) 607
BsmBI CGTCTC 1 cut(s) 607
BsmFI GGGAC 2 cut(s) 143, 481
BsnI GGCC 1 cut(s) 279
BsoBI CYCGRG 1 cut(s) 281
Bsp143I GATC 2 cut(s) 61, 102
BspACI CCGC 4 cut(s) 120, 437, 471, 579
BspANI GGCC 1 cut(s) 279
BspFNI CGCG 1 cut(s) 581
BspLI GGNNCC 1 cut(s) 469
BsrDI GCAATG 1 cut(s) 538
BsrFI RCCGGY 1 cut(s) 476
BssAI RCCGGY 1 cut(s) 476
BssMI GATC 2 cut(s) 61, 102
Bst2UI CCWGG 2 cut(s) 585, 593
Bst4CI ACNGT 4 cut(s) 157, 290, 388, 488
BstC8I GCNNGC 1 cut(s) 478
BstF5I GGATG 1 cut(s) 294
BstFNI CGCG 1 cut(s) 581
BstHHI GCGC 1 cut(s) 583
BstKTI GATC 2 cut(s) 64, 105
BstMAI GTCTC 1 cut(s) 607
BstMBI GATC 2 cut(s) 61, 102
BstMWI GCNNNNNNNGC 1 cut(s) 27
BstNI CCWGG 2 cut(s) 585, 593
BstSCI CCNGG 2 cut(s) 583, 591
BstUI CGCG 1 cut(s) 581
BstV1I GCAGC 3 cut(s) 39, 42, 460
BsuRI GGCC 1 cut(s) 279
BtsCI GGATG 1 cut(s) 294
BtsI GCAGTG 1 cut(s) 303
BtsIMutI CAGTG 3 cut(s) 295, 303, 493
Cac8I GCNNGC 1 cut(s) 478
CfoI GCGC 1 cut(s) 583
Cfr10I RCCGGY 1 cut(s) 476
Cfr13I GGNCC 1 cut(s) 468
Csp6I GTAC 2 cut(s) 271, 286
CviJI RGCY 9 cut(s) 21, 33, 79, 279, 393, 407, 553, 578, 596
CviKI_1 RGCY 9 cut(s) 21, 33, 79, 279, 393, 407, 553, 578, 596
CviQI GTAC 2 cut(s) 271, 286
DpnI GATC 2 cut(s) 63, 104
DpnII GATC 2 cut(s) 61, 102
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 468
Eco88I CYCGRG 1 cut(s) 281
EcoRII CCWGG 2 cut(s) 583, 591
Esp3I CGTCTC 1 cut(s) 607
FaiI YATR 4 cut(s) 107, 310, 315, 459
FalI AAGNNNNNCTT 2 cut(s) 316, 348
FaqI GGGAC 2 cut(s) 143, 481
FbaI TGATCA 1 cut(s) 102
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 4 cut(s) 28, 31, 474, 579
FokI GGATG 1 cut(s) 281
Fsp4HI GCNGC 4 cut(s) 28, 31, 474, 579
GlaI GCGC 1 cut(s) 582
GluI GCNGC 4 cut(s) 28, 31, 474, 579
HaeIII GGCC 1 cut(s) 279
HapII CCGG 2 cut(s) 477, 528
HhaI GCGC 1 cut(s) 583
Hin6I GCGC 1 cut(s) 581
HinP1I GCGC 1 cut(s) 581
HincII GTYRAC 2 cut(s) 175, 493
HindII GTYRAC 2 cut(s) 175, 493
HindIII AAGCTT 1 cut(s) 551
HinfI GANTC 2 cut(s) 148, 440
HpaII CCGG 2 cut(s) 477, 528
HphI GGTGA 3 cut(s) 241, 358, 359
Hpy166II GTNNAC 4 cut(s) 175, 349, 484, 493
Hpy188III TCNNGA 1 cut(s) 160
Hpy8I GTNNAC 4 cut(s) 175, 349, 484, 493
Hpy99I CGWCG 1 cut(s) 176
HpyAV CCTTC 1 cut(s) 51
HpyCH4III ACNGT 4 cut(s) 157, 290, 388, 488
HpyCH4IV ACGT 3 cut(s) 84, 518, 616
HpyCH4V TGCA 3 cut(s) 8, 308, 543
HpyF10VI GCNNNNNNNGC 1 cut(s) 27
HpySE526I ACGT 3 cut(s) 84, 518, 616
HspAI GCGC 1 cut(s) 581
KroI GCCGGC 1 cut(s) 476
KroNI GCCGGC 1 cut(s) 478
Ksp22I TGATCA 1 cut(s) 102
Kzo9I GATC 2 cut(s) 61, 102
LpnPI CCDG 9 cut(s) 93, 113, 490, 541, 570, 578, 592, 597, 605
Lsp1109I GCAGC 3 cut(s) 39, 42, 460
LweI GCATC 1 cut(s) 368
MaeII ACGT 3 cut(s) 84, 518, 616
MaeIII GTNAC 3 cut(s) 290, 301, 337
MalI GATC 2 cut(s) 63, 104
MboI GATC 2 cut(s) 61, 102
MluCI AATT 4 cut(s) 69, 164, 194, 445
MnlI CCTC 5 cut(s) 47, 251, 290, 533, 603
MroNI GCCGGC 1 cut(s) 476
MseI TTAA 3 cut(s) 450, 567, 625
MspA1I CMGCKG 1 cut(s) 473
MspI CCGG 2 cut(s) 477, 528
MspR9I CCNGG 2 cut(s) 585, 593
MvaI CCWGG 2 cut(s) 585, 593
MvnI CGCG 1 cut(s) 581
MwoI GCNNNNNNNGC 1 cut(s) 27
NaeI GCCGGC 1 cut(s) 478
NdeII GATC 2 cut(s) 61, 102
NgoMIV GCCGGC 1 cut(s) 476
NlaIV GGNNCC 1 cut(s) 469
NmuCI GTSAC 3 cut(s) 290, 301, 337
PaeR7I CTCGAG 1 cut(s) 281
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PdiI GCCGGC 1 cut(s) 478
PfeI GAWTC 2 cut(s) 148, 440
PkrI GCNGC 4 cut(s) 29, 32, 475, 580
Psp1406I AACGTT 1 cut(s) 518
Psp6I CCWGG 2 cut(s) 583, 591
PspGI CCWGG 2 cut(s) 583, 591
PspN4I GGNNCC 1 cut(s) 469
PspPI GGNCC 1 cut(s) 468
PspXI VCTCGAGB 1 cut(s) 281
RsaI GTAC 2 cut(s) 272, 287
RsaNI GTAC 2 cut(s) 271, 286
SalI GTCGAC 1 cut(s) 173
SaqAI TTAA 3 cut(s) 450, 567, 625
SatI GCNGC 4 cut(s) 28, 31, 474, 579
Sau3AI GATC 2 cut(s) 61, 102
Sau96I GGNCC 1 cut(s) 468
ScrFI CCNGG 2 cut(s) 585, 593
SfaNI GCATC 1 cut(s) 368
Sfr274I CTCGAG 1 cut(s) 281
SinI GGWCC 1 cut(s) 468
SlaI CTCGAG 1 cut(s) 281
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 4 cut(s) 69, 164, 194, 445
SsiI CCGC 4 cut(s) 120, 437, 471, 579
SspI AATATT 1 cut(s) 111
StyD4I CCNGG 2 cut(s) 583, 591
TaaI ACNGT 4 cut(s) 157, 290, 388, 488
TaiI ACGT 3 cut(s) 87, 521, 619
TaqI TCGA 2 cut(s) 174, 282
TasI AATT 4 cut(s) 69, 164, 194, 445
TatI WGTACW 2 cut(s) 270, 285
TauI GCSGC 1 cut(s) 581
TfiI GAWTC 2 cut(s) 148, 440
Tru1I TTAA 3 cut(s) 450, 567, 625
Tru9I TTAA 3 cut(s) 450, 567, 625
TscAI CASTG 3 cut(s) 295, 310, 493
TseFI GTSAC 3 cut(s) 290, 301, 337
TseI GCWGC 3 cut(s) 27, 30, 473
Tsp45I GTSAC 3 cut(s) 290, 301, 337
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 3 cut(s) 295, 310, 493
VpaK11BI GGWCC 1 cut(s) 468
XapI RAATTY 2 cut(s) 164, 445
XhoI CTCGAG 1 cut(s) 281
XmiI GTMKAC 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.