Prupe.5G186700_v2.0.a1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
N/A
Physical Location & Seq
Reverse (-)
15437497 .. 15438991
1495 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G186700.1_v2.0.a1.CDS.3

Sequence Viewer

Length: 984 bp
ATGTCCCTCAAATATTATTTAAGAGATCTCAATAGAAGGGATATATATGTGTCCGGCAAGCAACCCCAAACTTGGCATGCAGGGGAAATTAAGATTGACCCTGCATGCAGCTTCCTAAATCGATTATGTCTAGCTACTCCTATATATACAATCAATCAATGGGTTCACCACAAATTGGATCATCATCTTTTCAATGGGTTAATTTTGTTATTACAATTAAGGTTTCATCGGTTTGGTATTAGTGGATCGTTGATGGCAGATGTTAGAGGATCCACAAGATGGTCGACATCAACAATAGGCCTTCGTCGAAAAGTTGTACCTTTCTCAGAGTGGATGGAGGACTCCAAAGCCCATTATCTCCTCCTTCAACTTCCAAATTTCAAAAAGGGGGAGGTGAAGGTCGAGGTCAATGACTCAGCTGGCCATGTAACAATCAGCGGAGGAAGGCAAGTGAATAAAGAGAACGAATCTGGGTCTTCTGAGGAGGAGGAGGAGGAGGAGGATGAAGTTGAGTCTTTTGATGAGGAGGAGAGTGAATCTGATCAGTCTTCTGAGGATGAAATGTATTTTGTGGAGATTAAGAATGAATTTGAGTATTTTGAGGAGAAATTTTCCATTCCGCCAAACTCAGATGCCAATATGATCACGGCAAAATTGGAGGGTGAGACGCTCTATATCATTATACCAAAGGTAGGAGCAACAGAAAATGATACACAGTATACAAATGTCCAAGGCTTATTCTTAAGACCCGAAAATGATGGACACTACTCAAACATATTATTAGGAAATGGCCATGTATTGTTCTCTCTAGAAAATATAAGGAAATGGGATGAGCATGAAGATGGAGTTTTAAGGATGGCAATGGAGATGTTGAGCAAAAAGAAAAGGATGATTTTAACCGCCGCGACCGCCACCGCCGTTGTAGCATTTTCATTAGGGATGCTAGTTTCTCATCAGACGTTCCAATCAAGAGGAGGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

328

Amino Acids

37.39

Weight (kDa)

5.14

Isoelectric Point (pI)

55.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 175, 279
AccI GTMKAC 2 cut(s) 284, 719
AccII CGCG 1 cut(s) 905
AciI CCGC 6 cut(s) 438, 620, 900, 903, 909, 915
AclWI GGATC 4 cut(s) 186, 253, 264, 277
AcoI YGGCCR 2 cut(s) 421, 790
AcsI RAATTY 3 cut(s) 376, 587, 608
AfaI GTAC 1 cut(s) 318
AfiI CCNNNNNNNGG 4 cut(s) 72, 175, 279, 692
AflII CTTAAG 1 cut(s) 742
AgsI TTSAA 3 cut(s) 193, 368, 382
AluBI AGCT 3 cut(s) 111, 134, 419
AluI AGCT 3 cut(s) 111, 134, 419
Alw26I GTCTC 1 cut(s) 659
AlwI GGATC 4 cut(s) 186, 253, 264, 277
AoxI GGCC 3 cut(s) 298, 421, 790
ApeKI GCWGC 1 cut(s) 108
ApoI RAATTY 3 cut(s) 376, 587, 608
AsuHPI GGTGA 3 cut(s) 158, 406, 674
BalI TGGCCA 2 cut(s) 423, 792
BamHI GGATCC 1 cut(s) 269
BbsI GAAGAC 2 cut(s) 468, 540
BbvI GCAGC 1 cut(s) 120
BccI CCATC 6 cut(s) 247, 273, 328, 752, 836, 850
BceAI ACGGC 2 cut(s) 663, 902
BclI TGATCA 2 cut(s) 541, 642
BcoDI GTCTC 1 cut(s) 659
BfaI CTAG 3 cut(s) 131, 809, 944
BfrI CTTAAG 1 cut(s) 742
BglII AGATCT 1 cut(s) 25
BisI GCNGC 2 cut(s) 109, 903
BlsI GCNGC 2 cut(s) 110, 904
BmiI GGNNCC 1 cut(s) 271
BmsI GCATC 2 cut(s) 622, 930
BpiI GAAGAC 2 cut(s) 468, 540
Bsa29I ATCGAT 1 cut(s) 121
BsaBI GATNNNNATC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 730
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 175, 279, 692
Bse3DI GCAATG 1 cut(s) 867
Bse8I GATNNNNATC 1 cut(s) 183
BseCI ATCGAT 1 cut(s) 121
BseDI CCNNGG 1 cut(s) 730
BseGI GGATG 7 cut(s) 339, 508, 562, 835, 861, 894, 945
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 4 cut(s) 72, 175, 279, 692
BseMI GCAATG 1 cut(s) 867
BseMII CTCAG 5 cut(s) 339, 429, 471, 543, 642
BseXI GCAGC 1 cut(s) 120
Bsh1236I CGCG 1 cut(s) 905
Bsh1285I CGRYCG 1 cut(s) 909
BshFI GGCC 3 cut(s) 300, 423, 792
BshVI ATCGAT 1 cut(s) 121
BsiEI CGRYCG 1 cut(s) 909
BsiSI CCGG 1 cut(s) 54
BslI CCNNNNNNNGG 4 cut(s) 72, 175, 279, 692
BsmAI GTCTC 1 cut(s) 659
BsmBI CGTCTC 1 cut(s) 659
BsnI GGCC 3 cut(s) 300, 423, 792
Bsp143I GATC 6 cut(s) 25, 178, 245, 269, 541, 642
BspACI CCGC 6 cut(s) 438, 620, 900, 903, 909, 915
BspANI GGCC 3 cut(s) 300, 423, 792
BspCNI CTCAG 5 cut(s) 338, 428, 472, 544, 641
BspDI ATCGAT 1 cut(s) 121
BspFNI CGCG 1 cut(s) 905
BspLI GGNNCC 1 cut(s) 271
BspPI GGATC 4 cut(s) 186, 253, 264, 277
BspTI CTTAAG 1 cut(s) 742
BsrDI GCAATG 1 cut(s) 867
BssECI CCNNGG 1 cut(s) 730
BssMI GATC 6 cut(s) 25, 178, 245, 269, 541, 642
BssNAI GTATAC 1 cut(s) 720
BssT1I CCWWGG 1 cut(s) 730
Bst1107I GTATAC 1 cut(s) 720
Bst4CI ACNGT 1 cut(s) 717
BstAFI CTTAAG 1 cut(s) 742
BstC8I GCNNGC 4 cut(s) 59, 78, 106, 421
BstDEI CTNAG 5 cut(s) 325, 415, 480, 552, 628
BstF5I GGATG 7 cut(s) 339, 508, 562, 835, 861, 894, 945
BstFNI CGCG 1 cut(s) 905
BstKTI GATC 6 cut(s) 28, 181, 248, 272, 544, 645
BstMAI GTCTC 1 cut(s) 659
BstMBI GATC 6 cut(s) 25, 178, 245, 269, 541, 642
BstMCI CGRYCG 1 cut(s) 909
BstMWI GCNNNNNNNGC 2 cut(s) 908, 923
BstNSI RCATGY 2 cut(s) 80, 108
BstUI CGCG 1 cut(s) 905
BstV1I GCAGC 1 cut(s) 120
BstV2I GAAGAC 2 cut(s) 468, 540
BstX2I RGATCY 2 cut(s) 25, 269
BstYI RGATCY 2 cut(s) 25, 269
BstZ17I GTATAC 1 cut(s) 720
Bsu15I ATCGAT 1 cut(s) 121
BsuRI GGCC 3 cut(s) 300, 423, 792
BsuTUI ATCGAT 1 cut(s) 121
BtsCI GGATG 7 cut(s) 339, 508, 562, 835, 861, 894, 945
Cac8I GCNNGC 4 cut(s) 59, 78, 106, 421
ClaI ATCGAT 1 cut(s) 121
CseI GACGC 1 cut(s) 676
Csp6I GTAC 1 cut(s) 317
CspCI CAANNNNNGTGG 2 cut(s) 901, 936
CviAII CATG 5 cut(s) 77, 105, 425, 794, 836
CviJI RGCY 8 cut(s) 111, 134, 300, 350, 419, 423, 735, 792
CviKI_1 RGCY 8 cut(s) 111, 134, 300, 350, 419, 423, 735, 792
CviQI GTAC 1 cut(s) 317
DdeI CTNAG 5 cut(s) 325, 415, 480, 552, 628
DpnI GATC 6 cut(s) 27, 180, 247, 271, 543, 644
DpnII GATC 6 cut(s) 25, 178, 245, 269, 541, 642
EaeI YGGCCR 2 cut(s) 421, 790
EciI GGCGGA 1 cut(s) 609
Eco130I CCWWGG 1 cut(s) 730
Eco147I AGGCCT 1 cut(s) 300
EcoT14I CCWWGG 1 cut(s) 730
ErhI CCWWGG 1 cut(s) 730
Esp3I CGTCTC 1 cut(s) 659
FaeI CATG 5 cut(s) 80, 108, 428, 797, 839
FatI CATG 5 cut(s) 76, 104, 424, 793, 835
FbaI TGATCA 2 cut(s) 541, 642
FblI GTMKAC 2 cut(s) 284, 719
Fnu4HI GCNGC 2 cut(s) 109, 903
FokI GGATG 7 cut(s) 346, 515, 569, 842, 868, 901, 952
Fsp4HI GCNGC 2 cut(s) 109, 903
FspBI CTAG 3 cut(s) 131, 809, 944
GluI GCNGC 2 cut(s) 109, 903
HaeIII GGCC 3 cut(s) 300, 423, 792
HapII CCGG 1 cut(s) 54
HgaI GACGC 1 cut(s) 676
Hin1II CATG 5 cut(s) 80, 108, 428, 797, 839
HincII GTYRAC 1 cut(s) 285
HindII GTYRAC 1 cut(s) 285
HinfI GANTC 5 cut(s) 341, 413, 467, 512, 536
HpaII CCGG 1 cut(s) 54
HphI GGTGA 3 cut(s) 158, 406, 674
Hpy166II GTNNAC 3 cut(s) 166, 285, 720
Hpy188I TCNGA 6 cut(s) 328, 481, 541, 553, 631, 957
Hpy188III TCNNGA 2 cut(s) 809, 969
Hpy8I GTNNAC 3 cut(s) 166, 285, 720
Hpy99I CGWCG 1 cut(s) 309
HpyAV CCTTC 5 cut(s) 30, 311, 374, 391, 438
HpyCH4III ACNGT 1 cut(s) 717
HpyCH4IV ACGT 1 cut(s) 959
HpyCH4V TGCA 3 cut(s) 80, 104, 108
HpyF10VI GCNNNNNNNGC 2 cut(s) 908, 923
HpyF3I CTNAG 5 cut(s) 325, 415, 480, 552, 628
HpySE526I ACGT 1 cut(s) 959
Hsp92II CATG 5 cut(s) 80, 108, 428, 797, 839
Ksp22I TGATCA 2 cut(s) 541, 642
Kzo9I GATC 6 cut(s) 25, 178, 245, 269, 541, 642
LmnI GCTCC 1 cut(s) 695
LpnPI CCDG 5 cut(s) 66, 67, 114, 405, 456
Lsp1109I GCAGC 1 cut(s) 120
LweI GCATC 2 cut(s) 622, 930
MaeI CTAG 3 cut(s) 131, 809, 944
MaeII ACGT 1 cut(s) 959
MaeIII GTNAC 1 cut(s) 427
MalI GATC 6 cut(s) 27, 180, 247, 271, 543, 644
MboI GATC 6 cut(s) 25, 178, 245, 269, 541, 642
MboII GAAGA 3 cut(s) 468, 540, 851
MflI RGATCY 2 cut(s) 25, 269
MlsI TGGCCA 2 cut(s) 423, 792
MluCI AATT 8 cut(s) 87, 173, 201, 215, 376, 587, 608, 653
MluNI TGGCCA 2 cut(s) 423, 792
MlyI GAGTC 3 cut(s) 335, 407, 521
Mox20I TGGCCA 2 cut(s) 423, 792
MscI TGGCCA 2 cut(s) 423, 792
MseI TTAA 8 cut(s) 20, 90, 200, 218, 579, 743, 851, 896
MslI CAYNNNNRTG 1 cut(s) 840
Msp20I TGGCCA 2 cut(s) 423, 792
MspA1I CMGCKG 2 cut(s) 419, 438
MspCI CTTAAG 1 cut(s) 742
MspI CCGG 1 cut(s) 54
MvnI CGCG 1 cut(s) 905
MwoI GCNNNNNNNGC 2 cut(s) 908, 923
NdeII GATC 6 cut(s) 25, 178, 245, 269, 541, 642
NlaIII CATG 5 cut(s) 80, 108, 428, 797, 839
NlaIV GGNNCC 1 cut(s) 271
NspI RCATGY 2 cut(s) 80, 108
PaeI GCATGC 2 cut(s) 80, 108
PceI AGGCCT 1 cut(s) 300
PfeI GAWTC 2 cut(s) 467, 536
PflMI CCANNNNNTGG 2 cut(s) 175, 279
PkrI GCNGC 2 cut(s) 110, 904
PleI GAGTC 3 cut(s) 335, 407, 520
PpsI GAGTC 3 cut(s) 335, 407, 520
PspN4I GGNNCC 1 cut(s) 271
PsuI RGATCY 2 cut(s) 25, 269
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 1 cut(s) 318
RsaNI GTAC 1 cut(s) 317
RseI CAYNNNNRTG 1 cut(s) 840
SalI GTCGAC 1 cut(s) 283
SaqAI TTAA 8 cut(s) 20, 90, 200, 218, 579, 743, 851, 896
SatI GCNGC 2 cut(s) 109, 903
Sau3AI GATC 6 cut(s) 25, 178, 245, 269, 541, 642
SchI GAGTC 3 cut(s) 335, 407, 521
SfaNI GCATC 2 cut(s) 622, 930
SmiMI CAYNNNNRTG 1 cut(s) 840
SmlI CTYRAG 1 cut(s) 742
SmoI CTYRAG 1 cut(s) 742
SphI GCATGC 2 cut(s) 80, 108
Sse9I AATT 8 cut(s) 87, 173, 201, 215, 376, 587, 608, 653
SseBI AGGCCT 1 cut(s) 300
SsiI CCGC 6 cut(s) 438, 620, 900, 903, 909, 915
SspI AATATT 1 cut(s) 14
SspMI CTAG 3 cut(s) 131, 809, 944
StuI AGGCCT 1 cut(s) 300
StyI CCWWGG 1 cut(s) 730
TaaI ACNGT 1 cut(s) 717
TaiI ACGT 1 cut(s) 962
TaqI TCGA 4 cut(s) 121, 284, 307, 402
TasI AATT 8 cut(s) 87, 173, 201, 215, 376, 587, 608, 653
TauI GCSGC 1 cut(s) 905
TfiI GAWTC 2 cut(s) 467, 536
Tru1I TTAA 8 cut(s) 20, 90, 200, 218, 579, 743, 851, 896
Tru9I TTAA 8 cut(s) 20, 90, 200, 218, 579, 743, 851, 896
TseI GCWGC 1 cut(s) 108
TspDTI ATGAA 6 cut(s) 215, 519, 573, 600, 852, 921
Van91I CCANNNNNTGG 2 cut(s) 175, 279
Vha464I CTTAAG 1 cut(s) 742
XapI RAATTY 3 cut(s) 376, 587, 608
XbaI TCTAGA 1 cut(s) 808
XceI RCATGY 2 cut(s) 80, 108
XmiI GTMKAC 2 cut(s) 284, 719
XspI CTAG 3 cut(s) 131, 809, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.