Prupe.5G206900_v2.0.a1

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
N/A
Physical Location & Seq
Reverse (-)
16514122 .. 16517796
3675 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G206900.1_v2.0.a1.CDS.4

Sequence Viewer

Length: 333 bp
ATGAATCGTGCCTCGGCCTTGGCTCGCCTTCGCGCTCTTATAGCTCTAAAAGGAACACTGGATCTTGATGCTTATTCCCCTCCTCATGAGCTTCTTCAAATTCTTCCCCCAAAGTCTACCATTAGAGGATCAGATTGTGGTCCCCAAATTGGACCCAATAATCCTAAGTTCATTTTGGGAATGCAAGCTCTGTTGGATCTCATTTTTGCAGTTGAGGGTTCTGTCTCAGAGGCAGCCAAGTTTCTGGGGTTAAGCACAGGTGCCCTGTCACGGTTCATACTCTCAAATGATTGTCTCCGACTGGCACTCAATGAACTAAGCCCATACTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

111

Amino Acids

11.81

Weight (kDa)

7.8

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 260
AccI GTMKAC 1 cut(s) 116
AccII CGCG 1 cut(s) 33
AclWI GGATC 3 cut(s) 69, 136, 204
AcsI RAATTY 1 cut(s) 99
AfiI CCNNNNNNNGG 2 cut(s) 149, 270
AgsI TTSAA 1 cut(s) 98
AluBI AGCT 3 cut(s) 44, 91, 188
AluI AGCT 3 cut(s) 44, 91, 188
Alw26I GTCTC 2 cut(s) 229, 299
AlwI GGATC 3 cut(s) 69, 136, 204
AoxI GGCC 1 cut(s) 15
ApeKI GCWGC 1 cut(s) 233
ApoI RAATTY 1 cut(s) 99
AspLEI GCGC 1 cut(s) 35
AspS9I GGNCC 2 cut(s) 140, 152
AvaII GGWCC 2 cut(s) 140, 152
BaeGI GKGCMC 1 cut(s) 265
BanI GGYRCC 1 cut(s) 260
BbvI GCAGC 1 cut(s) 245
BcoDI GTCTC 2 cut(s) 229, 299
BisI GCNGC 1 cut(s) 234
BlsI GCNGC 1 cut(s) 235
Bme18I GGWCC 2 cut(s) 140, 152
BmgT120I GGNCC 2 cut(s) 140, 152
BmiI GGNNCC 3 cut(s) 142, 154, 262
BmsI GCATC 1 cut(s) 58
BsaJI CCNNGG 2 cut(s) 12, 18
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 270
Bse1I ACTGG 2 cut(s) 63, 306
BseDI CCNNGG 2 cut(s) 12, 18
BseLI CCNNNNNNNGG 2 cut(s) 149, 270
BseMII CTCAG 1 cut(s) 240
BseNI ACTGG 2 cut(s) 63, 306
BseRI GAGGAG 1 cut(s) 72
BseSI GKGCMC 1 cut(s) 265
BseXI GCAGC 1 cut(s) 245
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 1 cut(s) 17
BshNI GGYRCC 1 cut(s) 260
BslFI GGGAC 1 cut(s) 126
BslI CCNNNNNNNGG 2 cut(s) 149, 270
BsmAI GTCTC 2 cut(s) 229, 299
BsmFI GGGAC 1 cut(s) 126
BsmI GAATGC 1 cut(s) 186
BsnI GGCC 1 cut(s) 17
Bsp1286I GDGCHC 1 cut(s) 265
Bsp143I GATC 3 cut(s) 61, 128, 196
BspANI GGCC 1 cut(s) 17
BspCNI CTCAG 1 cut(s) 239
BspFNI CGCG 1 cut(s) 33
BspHI TCATGA 1 cut(s) 85
BspLI GGNNCC 3 cut(s) 142, 154, 262
BspPI GGATC 3 cut(s) 69, 136, 204
BspT107I GGYRCC 1 cut(s) 260
BsrI ACTGG 2 cut(s) 63, 306
BssECI CCNNGG 2 cut(s) 12, 18
BssMI GATC 3 cut(s) 61, 128, 196
BssT1I CCWWGG 1 cut(s) 18
Bst4CI ACNGT 1 cut(s) 273
BstC8I GCNNGC 2 cut(s) 25, 186
BstDEI CTNAG 3 cut(s) 165, 226, 317
BstFNI CGCG 1 cut(s) 33
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 3 cut(s) 64, 131, 199
BstMAI GTCTC 2 cut(s) 229, 299
BstMBI GATC 3 cut(s) 61, 128, 196
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstSLI GKGCMC 1 cut(s) 265
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 245
BstX2I RGATCY 2 cut(s) 61, 196
BstXI CCANNNNNNTGG 1 cut(s) 244
BstYI RGATCY 2 cut(s) 61, 196
BsuRI GGCC 1 cut(s) 17
BtsIMutI CAGTG 1 cut(s) 56
Cac8I GCNNGC 2 cut(s) 25, 186
CciI TCATGA 1 cut(s) 85
CfoI GCGC 1 cut(s) 35
Cfr13I GGNCC 2 cut(s) 140, 152
CviAII CATG 1 cut(s) 86
CviJI RGCY 7 cut(s) 17, 23, 44, 91, 188, 236, 321
CviKI_1 RGCY 7 cut(s) 17, 23, 44, 91, 188, 236, 321
DdeI CTNAG 3 cut(s) 165, 226, 317
DpnI GATC 3 cut(s) 63, 130, 198
DpnII GATC 3 cut(s) 61, 128, 196
Eco130I CCWWGG 1 cut(s) 18
Eco47I GGWCC 2 cut(s) 140, 152
EcoT14I CCWWGG 1 cut(s) 18
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 1 cut(s) 89
FaiI YATR 4 cut(s) 41, 87, 278, 325
FaqI GGGAC 1 cut(s) 126
FatI CATG 1 cut(s) 85
FblI GTMKAC 1 cut(s) 116
Fnu4HI GCNGC 1 cut(s) 234
Fsp4HI GCNGC 1 cut(s) 234
GlaI GCGC 1 cut(s) 34
GluI GCNGC 1 cut(s) 234
HaeIII GGCC 1 cut(s) 17
HhaI GCGC 1 cut(s) 35
Hin1II CATG 1 cut(s) 89
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HinfI GANTC 1 cut(s) 4
Hpy166II GTNNAC 1 cut(s) 117
Hpy188I TCNGA 3 cut(s) 133, 229, 299
Hpy188III TCNNGA 2 cut(s) 65, 86
Hpy8I GTNNAC 1 cut(s) 117
HpyAV CCTTC 1 cut(s) 38
HpyCH4III ACNGT 1 cut(s) 273
HpyCH4V TGCA 2 cut(s) 184, 209
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpyF3I CTNAG 3 cut(s) 165, 226, 317
Hsp92II CATG 1 cut(s) 89
HspAI GCGC 1 cut(s) 33
Kzo9I GATC 3 cut(s) 61, 128, 196
LpnPI CCDG 5 cut(s) 44, 230, 243, 278, 287
Lsp1109I GCAGC 1 cut(s) 245
LweI GCATC 1 cut(s) 58
MaeIII GTNAC 1 cut(s) 267
MalI GATC 3 cut(s) 63, 130, 198
MboI GATC 3 cut(s) 61, 128, 196
MboII GAAGA 2 cut(s) 86, 95
MflI RGATCY 2 cut(s) 61, 196
MhlI GDGCHC 1 cut(s) 265
MluCI AATT 2 cut(s) 99, 147
MmeI TCCRAC 2 cut(s) 174, 322
MnlI CCTC 6 cut(s) 22, 90, 93, 119, 208, 223
MseI TTAA 2 cut(s) 251, 331
Mva1269I GAATGC 1 cut(s) 186
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 1 cut(s) 41
NdeII GATC 3 cut(s) 61, 128, 196
NlaIII CATG 1 cut(s) 89
NlaIV GGNNCC 3 cut(s) 142, 154, 262
NmuCI GTSAC 1 cut(s) 267
PagI TCATGA 1 cut(s) 85
PctI GAATGC 1 cut(s) 186
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 1 cut(s) 235
PspN4I GGNNCC 3 cut(s) 142, 154, 262
PspPI GGNCC 2 cut(s) 140, 152
PsuI RGATCY 2 cut(s) 61, 196
SaqAI TTAA 2 cut(s) 251, 331
SatI GCNGC 1 cut(s) 234
Sau3AI GATC 3 cut(s) 61, 128, 196
Sau96I GGNCC 2 cut(s) 140, 152
SduI GDGCHC 1 cut(s) 265
SetI ASST 4 cut(s) 46, 93, 190, 262
SfaNI GCATC 1 cut(s) 58
SinI GGWCC 2 cut(s) 140, 152
Sse9I AATT 2 cut(s) 99, 147
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 1 cut(s) 273
TasI AATT 2 cut(s) 99, 147
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 2 cut(s) 251, 331
Tru9I TTAA 2 cut(s) 251, 331
TscAI CASTG 1 cut(s) 63
TseFI GTSAC 1 cut(s) 267
TseI GCWGC 1 cut(s) 233
Tsp45I GTSAC 1 cut(s) 267
TspDTI ATGAA 4 cut(s) 17, 160, 265, 327
TspRI CASTG 1 cut(s) 63
VpaK11BI GGWCC 2 cut(s) 140, 152
XapI RAATTY 1 cut(s) 99
XmiI GTMKAC 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.