Prupe.5G215200_v2.0.a1

Eukaryotic translation initiation factor 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
N/A
Physical Location & Seq
Reverse (-)
16933165 .. 16936948
3784 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G215200.1_v2.0.a1.CDS.3

Sequence Viewer

Length: 1089 bp
ATGGGTGTTAGCGACTCCGATTTTGATGATCGTATACGATTTTTCAGAACTAAAGCCCCTTCAGCTCTCAATCCCGTGTTCGCTTCTCAAACCAGAAAGAAAGCCGCGTTCACCGAAGAGAGCAATTTGTTTGTTGACTCTGAATGCAATGGTTGCATACAAATGACGGAATTGGATTTTTATCTAGATTTGATTTTGTCACGATATTCTTATGGCCACTATACTATGCTTAATGGTGCCACCATTATGGATGGAGCCTTACTTCTCAATGTTGCGAACGAAAGCTGTCCCCAACCACAAACTTCTGAGCATCTGGCTGCTGTTGAAATTATGTGTCTGCAACATATTATTATCCTTCAAAATAAAGTTGACCTGATTCAGGAGAATGTGGCCATCAATCAGCATGAGGGAACCATAGCTGATAATGCACCAGTGGTGCCAATTTCTGCTCAGTTGAAGTATAATATTGATGTTGTTTGCGAGTACATCGTCAAAAAGATCCCCTTTCCAGAAAGGAACTTTATCTCACCTCCCAATATGATTGTTATCCGTTCTTTTGATGTCAATAAACCTGGGTTCGAGGTCAATGAGATAAGAGGGTGTCTTGAAAATGAACCAATATATCGAGGAGGTCTCATTGGAGTTGGAACGACCATGGATCCTACTTTGACACGTGCTGATAGGCTAGTGGGTCAAGTTCTTGGTGAAGTTGGATCTCTCCCTGAAGTATTTGTTGAGCTTGAGATGATGTTTCCAAGGAAATTGAGCAAAGGGGCTAGGGTTATTGCTGTAAGGAATGATTTGGCAAAGTTGCAACTCACCTCTCCAGTTTGCACCAGCAGAGGAATTGCATTCAGTCGGCGAGTTGAGAAGCATTGTCGTCTTATTGGCCCCTACGCATGGAGCAAAGCGATTGGAACTGTTGTTGCGGGTGGAGACCTAGTGGCTAGTGTTGGTGATCCGTCCCGCCCGGAGCAAACTAAGAAAACATCTCTCTGGAAGAAGTTTCATCGGCACATGTACATGGCGGCAAATATTTGTCGGACCTCTCTTGACGAACATTACCTCTGCCGACAAAAGTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 eIF2_C

Protein Analysis

363

Amino Acids

40.5

Weight (kDa)

7.06

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 236, 436
AccI GTMKAC 1 cut(s) 34
AccII CGCG 1 cut(s) 107
AciI CCGC 4 cut(s) 105, 929, 967, 1028
AclWI GGATC 5 cut(s) 493, 653, 666, 721, 953
AcoI YGGCCR 2 cut(s) 214, 390
AcuI CTGAAG 2 cut(s) 45, 744
AcvI CACGTG 1 cut(s) 674
AfaI GTAC 2 cut(s) 485, 1022
AfiI CCNNNNNNNGG 2 cut(s) 379, 900
AflIII ACRYGT 2 cut(s) 671, 1017
AgsI TTSAA 4 cut(s) 326, 359, 457, 608
AjnI CCWGG 1 cut(s) 571
AluBI AGCT 4 cut(s) 65, 285, 419, 739
AluI AGCT 4 cut(s) 65, 285, 419, 739
Alw26I GTCTC 2 cut(s) 638, 930
AlwI GGATC 5 cut(s) 493, 653, 666, 721, 953
AoxI GGCC 3 cut(s) 214, 390, 889
ApeKI GCWGC 1 cut(s) 317
ArsI GACNNNNNNTTYG 1 cut(s) 37
AspS9I GGNCC 2 cut(s) 890, 1044
AsuC2I CCSGG 1 cut(s) 971
AsuHPI GGTGA 5 cut(s) 103, 519, 716, 811, 968
AvaII GGWCC 1 cut(s) 1044
BalI TGGCCA 2 cut(s) 216, 392
BamHI GGATCC 1 cut(s) 658
BanI GGYRCC 2 cut(s) 236, 436
BbrPI CACGTG 1 cut(s) 674
BbvI GCAGC 1 cut(s) 304
BccI CCATC 2 cut(s) 245, 401
BciT130I CCWGG 1 cut(s) 573
BcnI CCSGG 1 cut(s) 971
BcoDI GTCTC 2 cut(s) 638, 930
BfaI CTAG 5 cut(s) 185, 686, 777, 941, 948
BisI GCNGC 3 cut(s) 105, 318, 1029
BlsI GCNGC 3 cut(s) 106, 319, 1030
Bme1390I CCNGG 2 cut(s) 573, 971
Bme18I GGWCC 1 cut(s) 1044
BmgT120I GGNCC 2 cut(s) 890, 1044
BmiI GGNNCC 6 cut(s) 238, 256, 412, 438, 660, 892
BmrFI CCNGG 2 cut(s) 573, 971
BmsI GCATC 1 cut(s) 319
BplI GAGNNNNNCTC 2 cut(s) 618, 650
BpmI CTGGAG 1 cut(s) 810
BpuEI CTTGAG 1 cut(s) 761
BpuMI CCSGG 1 cut(s) 971
BsaAI YACGTR 1 cut(s) 674
BsaBI GATNNNNATC 2 cut(s) 180, 545
BsaI GGTCTC 2 cut(s) 638, 930
BsaJI CCNNGG 3 cut(s) 572, 654, 755
BsaXI ACNNNNNCTCC 2 cut(s) 927, 957
Bsc4I CCNNNNNNNGG 2 cut(s) 379, 900
Bse1I ACTGG 2 cut(s) 431, 827
Bse3DI GCAATG 1 cut(s) 154
Bse8I GATNNNNATC 2 cut(s) 180, 545
BseBI CCWGG 1 cut(s) 573
BseDI CCNNGG 3 cut(s) 572, 654, 755
BseGI GGATG 1 cut(s) 256
BseJI GATNNNNATC 2 cut(s) 180, 545
BseLI CCNNNNNNNGG 2 cut(s) 379, 900
BseMI GCAATG 1 cut(s) 154
BseMII CTCAG 2 cut(s) 297, 464
BseNI ACTGG 2 cut(s) 431, 827
BseRI GAGGAG 1 cut(s) 642
BseXI GCAGC 1 cut(s) 304
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 3 cut(s) 216, 392, 891
BshNI GGYRCC 2 cut(s) 236, 436
BsiSI CCGG 1 cut(s) 971
BslFI GGGAC 2 cut(s) 273, 949
BslI CCNNNNNNNGG 2 cut(s) 379, 900
BsmAI GTCTC 2 cut(s) 638, 930
BsmFI GGGAC 2 cut(s) 273, 949
BsmI GAATGC 2 cut(s) 149, 851
BsnI GGCC 3 cut(s) 216, 392, 891
Bso31I GGTCTC 2 cut(s) 638, 930
Bsp1407I TGTACA 1 cut(s) 1020
Bsp143I GATC 5 cut(s) 28, 498, 658, 713, 958
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 4 cut(s) 105, 929, 967, 1028
BspANI GGCC 3 cut(s) 216, 392, 891
BspCNI CTCAG 2 cut(s) 298, 463
BspFNI CGCG 1 cut(s) 107
BspLI GGNNCC 6 cut(s) 238, 256, 412, 438, 660, 892
BspPI GGATC 5 cut(s) 493, 653, 666, 721, 953
BspT107I GGYRCC 2 cut(s) 236, 436
BspTNI GGTCTC 2 cut(s) 638, 930
BsrDI GCAATG 1 cut(s) 154
BsrGI TGTACA 1 cut(s) 1020
BsrI ACTGG 2 cut(s) 431, 827
BssECI CCNNGG 3 cut(s) 572, 654, 755
BssMI GATC 5 cut(s) 28, 498, 658, 713, 958
BssNAI GTATAC 1 cut(s) 35
BssT1I CCWWGG 2 cut(s) 654, 755
Bst1107I GTATAC 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 573
Bst4CI ACNGT 1 cut(s) 922
Bst6I CTCTTC 1 cut(s) 111
BstAPI GCANNNNNTGC 1 cut(s) 153
BstAUI TGTACA 1 cut(s) 1020
BstBAI YACGTR 1 cut(s) 674
BstDEI CTNAG 3 cut(s) 306, 450, 981
BstDSI CCRYGG 1 cut(s) 654
BstENI CCTNNNNNAGG 1 cut(s) 377
BstF5I GGATG 1 cut(s) 256
BstFNI CGCG 1 cut(s) 107
BstKTI GATC 5 cut(s) 31, 501, 661, 716, 961
BstMAI GTCTC 2 cut(s) 638, 930
BstMBI GATC 5 cut(s) 28, 498, 658, 713, 958
BstMWI GCNNNNNNNGC 3 cut(s) 62, 153, 425
BstNI CCWGG 1 cut(s) 573
BstNSI RCATGY 1 cut(s) 1021
BstSCI CCNGG 2 cut(s) 571, 969
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 1 cut(s) 304
BstX2I RGATCY 3 cut(s) 498, 658, 713
BstXI CCANNNNNNTGG 1 cut(s) 247
BstYI RGATCY 3 cut(s) 498, 658, 713
BstZ17I GTATAC 1 cut(s) 35
BsuRI GGCC 3 cut(s) 216, 392, 891
BtgI CCRYGG 1 cut(s) 654
BtsCI GGATG 1 cut(s) 256
BtsIMutI CAGTG 1 cut(s) 438
Cfr13I GGNCC 2 cut(s) 890, 1044
Csp6I GTAC 2 cut(s) 484, 1021
CviAII CATG 5 cut(s) 404, 655, 900, 1018, 1024
CviQI GTAC 2 cut(s) 484, 1021
DdeI CTNAG 3 cut(s) 306, 450, 981
DpnI GATC 5 cut(s) 30, 500, 660, 715, 960
DpnII GATC 5 cut(s) 28, 498, 658, 713, 958
EaeI YGGCCR 2 cut(s) 214, 390
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
Eco130I CCWWGG 2 cut(s) 654, 755
Eco31I GGTCTC 2 cut(s) 638, 930
Eco47I GGWCC 1 cut(s) 1044
Eco57I CTGAAG 2 cut(s) 45, 744
Eco72I CACGTG 1 cut(s) 674
EcoNI CCTNNNNNAGG 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 571
EcoT14I CCWWGG 2 cut(s) 654, 755
ErhI CCWWGG 2 cut(s) 654, 755
FaeI CATG 5 cut(s) 407, 658, 903, 1021, 1027
FalI AAGNNNNNCTT 2 cut(s) 488, 520
FaqI GGGAC 2 cut(s) 273, 949
FatI CATG 5 cut(s) 403, 654, 899, 1017, 1023
FauI CCCGC 2 cut(s) 922, 974
FblI GTMKAC 1 cut(s) 34
Fnu4HI GCNGC 3 cut(s) 105, 318, 1029
FokI GGATG 1 cut(s) 263
Fsp4HI GCNGC 3 cut(s) 105, 318, 1029
FspBI CTAG 5 cut(s) 185, 686, 777, 941, 948
GluI GCNGC 3 cut(s) 105, 318, 1029
GsuI CTGGAG 1 cut(s) 810
HaeIII GGCC 3 cut(s) 216, 392, 891
HapII CCGG 1 cut(s) 971
Hin1II CATG 5 cut(s) 407, 658, 903, 1021, 1027
HincII GTYRAC 2 cut(s) 136, 370
HindII GTYRAC 2 cut(s) 136, 370
HinfI GANTC 3 cut(s) 14, 137, 376
HpaII CCGG 1 cut(s) 971
HphI GGTGA 5 cut(s) 103, 519, 716, 811, 968
Hpy166II GTNNAC 4 cut(s) 35, 111, 136, 370
Hpy188I TCNGA 6 cut(s) 19, 47, 142, 307, 1044, 1088
Hpy188III TCNNGA 7 cut(s) 185, 201, 380, 509, 605, 997, 1052
Hpy8I GTNNAC 4 cut(s) 35, 111, 136, 370
HpyAV CCTTC 2 cut(s) 69, 365
HpyCH4III ACNGT 1 cut(s) 922
HpyCH4IV ACGT 1 cut(s) 673
HpyCH4V TGCA 7 cut(s) 147, 156, 340, 428, 814, 834, 851
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 153, 425
HpyF3I CTNAG 3 cut(s) 306, 450, 981
HpySE526I ACGT 1 cut(s) 673
Hsp92II CATG 5 cut(s) 407, 658, 903, 1021, 1027
Kzo9I GATC 5 cut(s) 28, 498, 658, 713, 958
LmnI GCTCC 3 cut(s) 254, 903, 973
Lsp1109I GCAGC 1 cut(s) 304
LweI GCATC 1 cut(s) 319
MaeI CTAG 5 cut(s) 185, 686, 777, 941, 948
MaeII ACGT 1 cut(s) 673
MaeIII GTNAC 1 cut(s) 198
MalI GATC 5 cut(s) 30, 500, 660, 715, 960
MboI GATC 5 cut(s) 28, 498, 658, 713, 958
MboII GAAGA 3 cut(s) 128, 1012, 1075
MflI RGATCY 3 cut(s) 498, 658, 713
MlsI TGGCCA 2 cut(s) 216, 392
MluCI AATT 6 cut(s) 124, 170, 327, 441, 761, 846
MluNI TGGCCA 2 cut(s) 216, 392
MlyI GAGTC 2 cut(s) 8, 131
MmeI TCCRAC 3 cut(s) 625, 691, 1022
Mox20I TGGCCA 2 cut(s) 216, 392
MscI TGGCCA 2 cut(s) 216, 392
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 3 cut(s) 161, 245, 1022
Msp20I TGGCCA 2 cut(s) 216, 392
MspI CCGG 1 cut(s) 971
MspR9I CCNGG 2 cut(s) 573, 971
Mva1269I GAATGC 2 cut(s) 149, 851
MvaI CCWGG 1 cut(s) 573
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 3 cut(s) 62, 153, 425
NciI CCSGG 1 cut(s) 971
NcoI CCATGG 1 cut(s) 654
NdeII GATC 5 cut(s) 28, 498, 658, 713, 958
NlaIII CATG 5 cut(s) 407, 658, 903, 1021, 1027
NlaIV GGNNCC 6 cut(s) 238, 256, 412, 438, 660, 892
NmuCI GTSAC 1 cut(s) 198
NspI RCATGY 1 cut(s) 1021
PciI ACATGT 1 cut(s) 1017
PctI GAATGC 2 cut(s) 149, 851
PfeI GAWTC 1 cut(s) 376
PkrI GCNGC 3 cut(s) 106, 319, 1030
PleI GAGTC 2 cut(s) 8, 131
PmaCI CACGTG 1 cut(s) 674
PmlI CACGTG 1 cut(s) 674
PpsI GAGTC 2 cut(s) 8, 131
Ppu21I YACGTR 1 cut(s) 674
PscI ACATGT 1 cut(s) 1017
Psp6I CCWGG 1 cut(s) 571
PspCI CACGTG 1 cut(s) 674
PspGI CCWGG 1 cut(s) 571
PspN4I GGNNCC 6 cut(s) 238, 256, 412, 438, 660, 892
PspPI GGNCC 2 cut(s) 890, 1044
PsuI RGATCY 3 cut(s) 498, 658, 713
RsaI GTAC 2 cut(s) 485, 1022
RsaNI GTAC 2 cut(s) 484, 1021
RseI CAYNNNNRTG 3 cut(s) 161, 245, 1022
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 3 cut(s) 105, 318, 1029
Sau3AI GATC 5 cut(s) 28, 498, 658, 713, 958
Sau96I GGNCC 2 cut(s) 890, 1044
SchI GAGTC 2 cut(s) 8, 131
ScrFI CCNGG 2 cut(s) 573, 971
SfaNI GCATC 1 cut(s) 319
SinI GGWCC 1 cut(s) 1044
SmiMI CAYNNNNRTG 3 cut(s) 161, 245, 1022
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 6 cut(s) 124, 170, 327, 441, 761, 846
SsiI CCGC 4 cut(s) 105, 929, 967, 1028
SspI AATATT 2 cut(s) 466, 1036
SspMI CTAG 5 cut(s) 185, 686, 777, 941, 948
StyD4I CCNGG 2 cut(s) 571, 969
StyI CCWWGG 2 cut(s) 654, 755
TaaI ACNGT 1 cut(s) 922
TaiI ACGT 1 cut(s) 676
TaqI TCGA 2 cut(s) 579, 625
TasI AATT 6 cut(s) 124, 170, 327, 441, 761, 846
TatI WGTACW 2 cut(s) 483, 1020
TauI GCSGC 2 cut(s) 107, 1031
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 1 cut(s) 438
TseFI GTSAC 1 cut(s) 198
TseI GCWGC 1 cut(s) 317
Tsp45I GTSAC 1 cut(s) 198
TspDTI ATGAA 2 cut(s) 627, 998
TspGWI ACGGA 3 cut(s) 182, 539, 951
TspRI CASTG 1 cut(s) 438
VpaK11BI GGWCC 1 cut(s) 1044
XagI CCTNNNNNAGG 1 cut(s) 377
XbaI TCTAGA 1 cut(s) 184
XceI RCATGY 1 cut(s) 1021
XmiI GTMKAC 1 cut(s) 34
XspI CTAG 5 cut(s) 185, 686, 777, 941, 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.