Prupe.6G066600_v2.0.a1

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Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
N/A
Physical Location & Seq
Forward (+)
4588026 .. 4588390
365 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G066600.1_v2.0.a1.CDS.2

Sequence Viewer

Length: 264 bp
ATGTCGATCATCCCAAGCTTTCCACGAAGCAGCATTTTCGACCCCTTCAAGGATATCCCAGTTCCTTCTTCCTCATCACTTTCCACAGGAAAATTCAGCTTTCCTGAATCGACTGGAAGGAGAGCCCAGAAGCCCACGTTTCAGCTCCCGGAGAATGCGAAGGTAGACGAGATTAAGGCTGCAATGGAGAATGGGGCTCTGAGCGTCACTGTTCCCAAGGCAGAGGTGAAAAAGGCCGATGTTAAAGCCATTGAAATTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

88

Amino Acids

9.41

Weight (kDa)

9.1

Isoelectric Point (pI)

57.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 165
AcsI RAATTY 1 cut(s) 92
AfiI CCNNNNNNNGG 1 cut(s) 49
AgsI TTSAA 2 cut(s) 49, 254
AluBI AGCT 3 cut(s) 18, 99, 145
AluI AGCT 3 cut(s) 18, 99, 145
AoxI GGCC 1 cut(s) 234
ApeKI GCWGC 2 cut(s) 30, 179
ApoI RAATTY 1 cut(s) 92
AseI ATTAAT 1 cut(s) 258
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 1 cut(s) 238
BanII GRGCYC 2 cut(s) 127, 199
BbvI GCAGC 2 cut(s) 42, 166
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BcnI CCSGG 1 cut(s) 149
BisI GCNGC 2 cut(s) 31, 180
BlsI GCNGC 2 cut(s) 32, 181
Bme1390I CCNGG 1 cut(s) 149
BmrFI CCNGG 1 cut(s) 149
BmrI ACTGGG 1 cut(s) 53
BmuI ACTGGG 1 cut(s) 53
BpuMI CCSGG 1 cut(s) 149
BsaJI CCNNGG 1 cut(s) 216
Bsc4I CCNNNNNNNGG 1 cut(s) 49
Bse1I ACTGG 2 cut(s) 59, 118
Bse3DI GCAATG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 49
BseMI GCAATG 1 cut(s) 189
BseMII CTCAG 1 cut(s) 191
BseNI ACTGG 2 cut(s) 59, 118
BseXI GCAGC 2 cut(s) 42, 166
BshFI GGCC 1 cut(s) 236
BsiSI CCGG 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 49
BsmI GAATGC 1 cut(s) 160
BsnI GGCC 1 cut(s) 236
Bsp1286I GDGCHC 2 cut(s) 127, 199
Bsp143I GATC 1 cut(s) 6
BspANI GGCC 1 cut(s) 236
BspCNI CTCAG 1 cut(s) 192
BsrDI GCAATG 1 cut(s) 189
BsrI ACTGG 2 cut(s) 59, 118
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 216
Bst4CI ACNGT 1 cut(s) 211
BstDEI CTNAG 1 cut(s) 200
BstF5I GGATG 1 cut(s) 9
BstKTI GATC 1 cut(s) 9
BstMBI GATC 1 cut(s) 6
BstSCI CCNGG 1 cut(s) 147
BstV1I GCAGC 2 cut(s) 42, 166
BsuRI GGCC 1 cut(s) 236
BtsCI GGATG 1 cut(s) 9
BtsIMutI CAGTG 1 cut(s) 207
CseI GACGC 1 cut(s) 193
CviJI RGCY 9 cut(s) 18, 99, 125, 133, 145, 179, 197, 236, 248
CviKI_1 RGCY 9 cut(s) 18, 99, 125, 133, 145, 179, 197, 236, 248
DdeI CTNAG 1 cut(s) 200
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
Eco130I CCWWGG 1 cut(s) 216
Eco24I GRGCYC 2 cut(s) 127, 199
Eco32I GATATC 1 cut(s) 55
EcoRV GATATC 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 216
EcoT38I GRGCYC 2 cut(s) 127, 199
ErhI CCWWGG 1 cut(s) 216
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 2 cut(s) 31, 180
FriOI GRGCYC 2 cut(s) 127, 199
Fsp4HI GCNGC 2 cut(s) 31, 180
GluI GCNGC 2 cut(s) 31, 180
HaeIII GGCC 1 cut(s) 236
HapII CCGG 1 cut(s) 149
HgaI GACGC 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 16
HinfI GANTC 1 cut(s) 107
HpaII CCGG 1 cut(s) 149
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 1 cut(s) 201
Hpy188III TCNNGA 1 cut(s) 104
Hpy8I GTNNAC 1 cut(s) 166
HpyAV CCTTC 4 cut(s) 55, 75, 111, 154
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4IV ACGT 1 cut(s) 137
HpyCH4V TGCA 1 cut(s) 182
HpyF3I CTNAG 1 cut(s) 200
HpySE526I ACGT 1 cut(s) 137
Kzo9I GATC 1 cut(s) 6
LmnI GCTCC 1 cut(s) 150
LpnPI CCDG 6 cut(s) 72, 72, 99, 117, 140, 162
Lsp1109I GCAGC 2 cut(s) 42, 166
MaeII ACGT 1 cut(s) 137
MaeIII GTNAC 1 cut(s) 205
MalI GATC 1 cut(s) 8
MboI GATC 1 cut(s) 6
MboII GAAGA 1 cut(s) 60
MhlI GDGCHC 2 cut(s) 127, 199
MluCI AATT 3 cut(s) 92, 255, 259
MnlI CCTC 2 cut(s) 82, 217
MseI TTAA 3 cut(s) 174, 243, 258
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 1 cut(s) 149
Mva1269I GAATGC 1 cut(s) 160
NciI CCSGG 1 cut(s) 149
NdeII GATC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 205
PctI GAATGC 1 cut(s) 160
PfeI GAWTC 1 cut(s) 107
PfoI TCCNGGA 1 cut(s) 147
PkrI GCNGC 2 cut(s) 32, 181
PshBI ATTAAT 1 cut(s) 258
SaqAI TTAA 3 cut(s) 174, 243, 258
SatI GCNGC 2 cut(s) 31, 180
Sau3AI GATC 1 cut(s) 6
ScrFI CCNGG 1 cut(s) 149
SduI GDGCHC 2 cut(s) 127, 199
SetI ASST 6 cut(s) 20, 101, 140, 147, 165, 228
Sse9I AATT 3 cut(s) 92, 255, 259
StyD4I CCNGG 1 cut(s) 147
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 1 cut(s) 211
TaiI ACGT 1 cut(s) 140
TaqI TCGA 3 cut(s) 5, 39, 110
TasI AATT 3 cut(s) 92, 255, 259
TfiI GAWTC 1 cut(s) 107
Tru1I TTAA 3 cut(s) 174, 243, 258
Tru9I TTAA 3 cut(s) 174, 243, 258
TscAI CASTG 1 cut(s) 214
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 2 cut(s) 30, 179
Tsp45I GTSAC 1 cut(s) 205
TspRI CASTG 1 cut(s) 214
VspI ATTAAT 1 cut(s) 258
XapI RAATTY 1 cut(s) 92
XmiI GTMKAC 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.