Prupe.6G202500_v2.0.a1

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
N/A
Physical Location & Seq
Forward (+)
21014620 .. 21019112
4493 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G202500.1_v2.0.a1.CDS.4

Sequence Viewer

Length: 1215 bp
ATGGCAAAGTCATCATGCAGCCTCATTCTTATTCTCTCATGTGTACTTGTGTTTGCCTTCATGAACAACTCATCAGCCGTAGCGTCCAGTGGTATTAATAATTACCGTGTGGTCAACTTAGGAGCCAAACCAGATGGCAAGACTGACTCAACCAAAGCCTTCCTTTCTGCATGGGACAAAGCTTGTGCCTCCGTGAAGCCAGCTGTCGTTCACGTCCCGCGCGGGACGTTCTTGCTAGGCAATGCCATCTTCAGAGGGCCATGCAACAACAAGGCCACCACCTTTCGCATCTCCGAAGGCACCCTCGTTGCCCCATCCGATTACCGGGTCCTTGGAAATGCTGGTAACTGGCTTTCGTTTCAGGATGTGCACGGCCTCAACATATCTAGCTCGAACGGAATTCTTGATGGCCAAGGCACTGGTTTGTGGGATTGCAAGGCCTCACCAGGCAAGAGTAATTGCCCTACCGGAGCAATCTCACTGGGTTTCGTCAACTCGAGCAACATTGTGGTGAGTGGATTGCTATCCCGAAACAGCCAATTGGTCCACATAATCATCAGAGGTTGTCACAATGTGAATATGCATGGAATTAGGGTTTCTGCCTCTGAAAAAAGCCCCAACACCGATGGCATCCACATTCAAATGTCATCAGACATCACCATTTTGAACTCTGATTTTTCAACCGGTGATGATTGTGTCTCAATTGGCCCTGGCACCGTTAACTTGTGGATCGAAAAGGTTACTTGTGGACCCGGCCATGGAATTAGCGTTGGAAGTCTAGGGTGGGAGATGCAAGAGCCTGGTGTGCAAAATGTAACAGTTAAAACTGCTACATTTAAGAATACCTTGAATGGTGTCAGGATTAAGTCTTGGAGTAGGCCTAGCACTGGATTTGCTAGGCACATTCTCTTCCAACACGCCGTAATGGTCGATGTCAAAAATCCCATCATTATTGATCAAAACTACTGCCCTAACAACAAAGACTGCCCTCGTCAGGTTTCTGGAATCGAAATTACCGACGTGACGTACCAAGACATCCAGGGTACATCAGCAACTGAAGTTGCAGTGAATTTCGATTGTAGTTCCAAGAATCCTTGTACGGGGATTAGATTGGAGGATGTGAAGCTGACTTACCAGAACCAACCATCTGAAGCTTCATGTAGCCATGCAGATGGAACAGCTGCAGGTATAGTTCAGCCTACAAGTTGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

405

Amino Acids

42.85

Weight (kDa)

7.88

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1175
AccB1I GGYRCC 2 cut(s) 299, 713
AccII CGCG 2 cut(s) 220, 222
AciI CCGC 2 cut(s) 218, 222
AclWI GGATC 1 cut(s) 737
AcoI YGGCCR 2 cut(s) 409, 754
AcsI RAATTY 2 cut(s) 399, 1069
AcuI CTGAAG 3 cut(s) 235, 1077, 1170
AdeI CACNNNGTG 1 cut(s) 574
AfaI GTAC 4 cut(s) 45, 1028, 1045, 1099
AfiI CCNNNNNNNGG 5 cut(s) 324, 758, 887, 994, 1100
AgeI ACCGGT 1 cut(s) 683
AgsI TTSAA 4 cut(s) 641, 667, 681, 850
AjiI CACGTC 2 cut(s) 214, 1021
AjnI CCWGG 4 cut(s) 445, 709, 799, 1038
AluBI AGCT 6 cut(s) 182, 203, 390, 1126, 1154, 1181
AluI AGCT 6 cut(s) 182, 203, 390, 1126, 1154, 1181
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 1 cut(s) 703
Alw44I GTGCAC 1 cut(s) 368
AlwI GGATC 1 cut(s) 737
AlwNI CAGNNNCTG 1 cut(s) 1055
Ama87I CYCGRG 1 cut(s) 496
AoxI GGCC 8 cut(s) 257, 273, 373, 409, 438, 706, 754, 878
ApaLI GTGCAC 1 cut(s) 368
ApeKI GCWGC 2 cut(s) 18, 1181
ApoI RAATTY 2 cut(s) 399, 1069
AseI ATTAAT 1 cut(s) 96
AsiGI ACCGGT 1 cut(s) 683
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 5 cut(s) 257, 328, 544, 707, 749
AsuC2I CCSGG 2 cut(s) 326, 753
AsuHPI GGTGA 4 cut(s) 435, 523, 649, 698
AvaI CYCGRG 1 cut(s) 496
AvaII GGWCC 3 cut(s) 328, 544, 749
BaeGI GKGCMC 1 cut(s) 372
BalI TGGCCA 1 cut(s) 411
BanI GGYRCC 2 cut(s) 299, 713
BarI GAAGNNNNNNTAC 2 cut(s) 1115, 1147
Bbv12I GWGCWC 1 cut(s) 372
BbvI GCAGC 2 cut(s) 30, 1168
BccI CCATC 8 cut(s) 128, 254, 322, 401, 620, 953, 1153, 1166
BceAI ACGGC 3 cut(s) 62, 388, 905
BciT130I CCWGG 4 cut(s) 447, 711, 801, 1040
BclI TGATCA 1 cut(s) 955
BcnI CCSGG 2 cut(s) 326, 753
BcoDI GTCTC 1 cut(s) 703
BfaI CTAG 5 cut(s) 236, 387, 779, 882, 897
BfmI CTRYAG 1 cut(s) 1182
BfuAI ACCTGC 1 cut(s) 1175
BisI GCNGC 2 cut(s) 19, 1182
BlsI GCNGC 2 cut(s) 20, 1183
Bme1390I CCNGG 6 cut(s) 326, 447, 711, 753, 801, 1040
Bme18I GGWCC 3 cut(s) 328, 544, 749
BmeT110I CYCGRG 1 cut(s) 496
BmgBI CACGTC 2 cut(s) 214, 1021
BmgT120I GGNCC 5 cut(s) 257, 328, 544, 707, 749
BmiI GGNNCC 5 cut(s) 124, 301, 329, 715, 751
BmrFI CCNGG 6 cut(s) 326, 447, 711, 753, 801, 1040
BmrI ACTGGG 1 cut(s) 491
BmsI GCATC 3 cut(s) 297, 639, 780
BmuI ACTGGG 1 cut(s) 491
BpuMI CCSGG 2 cut(s) 326, 753
BsaBI GATNNNNATC 1 cut(s) 523
BsaJI CCNNGG 5 cut(s) 331, 412, 709, 757, 1039
BsaWI WCCGGW 2 cut(s) 467, 683
Bsc4I CCNNNNNNNGG 5 cut(s) 324, 758, 887, 994, 1100
Bse118I RCCGGY 1 cut(s) 683
Bse1I ACTGG 5 cut(s) 87, 353, 424, 486, 892
Bse3DI GCAATG 1 cut(s) 247
Bse8I GATNNNNATC 1 cut(s) 523
BseBI CCWGG 4 cut(s) 447, 711, 801, 1040
BseDI CCNNGG 5 cut(s) 331, 412, 709, 757, 1039
BseGI GGATG 5 cut(s) 314, 370, 630, 1035, 1123
BseJI GATNNNNATC 1 cut(s) 523
BseLI CCNNNNNNNGG 5 cut(s) 324, 758, 887, 994, 1100
BseMI GCAATG 1 cut(s) 247
BseNI ACTGG 5 cut(s) 87, 353, 424, 486, 892
BseSI GKGCMC 1 cut(s) 372
BseXI GCAGC 2 cut(s) 30, 1168
Bsh1236I CGCG 2 cut(s) 220, 222
BshFI GGCC 8 cut(s) 259, 275, 375, 411, 440, 708, 756, 880
BshNI GGYRCC 2 cut(s) 299, 713
BshTI ACCGGT 1 cut(s) 683
BsiHKAI GWGCWC 1 cut(s) 372
BsiHKCI CYCGRG 1 cut(s) 496
BsiSI CCGG 4 cut(s) 325, 468, 684, 753
BslFI GGGAC 3 cut(s) 188, 200, 238
BslI CCNNNNNNNGG 5 cut(s) 324, 758, 887, 994, 1100
BsmAI GTCTC 1 cut(s) 703
BsmFI GGGAC 3 cut(s) 188, 200, 238
BsnI GGCC 8 cut(s) 259, 275, 375, 411, 440, 708, 756, 880
BsoBI CYCGRG 1 cut(s) 496
Bsp1286I GDGCHC 1 cut(s) 372
Bsp143I GATC 2 cut(s) 729, 955
Bsp19I CCATGG 1 cut(s) 757
BspACI CCGC 2 cut(s) 218, 222
BspANI GGCC 8 cut(s) 259, 275, 375, 411, 440, 708, 756, 880
BspFNI CGCG 2 cut(s) 220, 222
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 5 cut(s) 124, 301, 329, 715, 751
BspMAI CTGCAG 1 cut(s) 1186
BspMI ACCTGC 1 cut(s) 1175
BspPI GGATC 1 cut(s) 737
BspT107I GGYRCC 2 cut(s) 299, 713
BsrDI GCAATG 1 cut(s) 247
BsrFI RCCGGY 1 cut(s) 683
BsrI ACTGG 5 cut(s) 87, 353, 424, 486, 892
BssAI RCCGGY 1 cut(s) 683
BssECI CCNNGG 5 cut(s) 331, 412, 709, 757, 1039
BssMI GATC 2 cut(s) 729, 955
BssT1I CCWWGG 3 cut(s) 331, 412, 757
Bst2UI CCWGG 4 cut(s) 447, 711, 801, 1040
Bst4CI ACNGT 3 cut(s) 107, 718, 820
Bst6I CTCTTC 1 cut(s) 914
BstC8I GCNNGC 1 cut(s) 201
BstDEI CTNAG 1 cut(s) 118
BstDSI CCRYGG 1 cut(s) 757
BstF5I GGATG 5 cut(s) 314, 370, 630, 1035, 1123
BstFNI CGCG 2 cut(s) 220, 222
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 2 cut(s) 732, 958
BstMAI GTCTC 1 cut(s) 703
BstMBI GATC 2 cut(s) 729, 955
BstMWI GCNNNNNNNGC 1 cut(s) 805
BstNI CCWGG 4 cut(s) 447, 711, 801, 1040
BstSCI CCNGG 6 cut(s) 324, 445, 709, 751, 799, 1038
BstSFI CTRYAG 1 cut(s) 1182
BstSLI GKGCMC 1 cut(s) 372
BstUI CGCG 2 cut(s) 220, 222
BstV1I GCAGC 2 cut(s) 30, 1168
BstXI CCANNNNNNTGG 2 cut(s) 419, 1172
BsuRI GGCC 8 cut(s) 259, 275, 375, 411, 440, 708, 756, 880
BtgI CCRYGG 1 cut(s) 757
BtrI CACGTC 2 cut(s) 214, 1021
BtsCI GGATG 5 cut(s) 314, 370, 630, 1035, 1123
BtsI GCAGTG 1 cut(s) 1071
BtsIMutI CAGTG 5 cut(s) 94, 417, 479, 885, 1071
BveI ACCTGC 1 cut(s) 1175
Cac8I GCNNGC 1 cut(s) 201
CaiI CAGNNNCTG 1 cut(s) 1055
CciI TCATGA 1 cut(s) 60
CfoI GCGC 1 cut(s) 222
Cfr10I RCCGGY 1 cut(s) 683
Cfr13I GGNCC 5 cut(s) 257, 328, 544, 707, 749
CseI GACGC 1 cut(s) 72
Csp6I GTAC 4 cut(s) 44, 1027, 1044, 1098
CspAI ACCGGT 1 cut(s) 683
CviAII CATG 9 cut(s) 15, 39, 61, 171, 261, 584, 758, 1158, 1166
CviQI GTAC 4 cut(s) 44, 1027, 1044, 1098
DdeI CTNAG 1 cut(s) 118
DpnI GATC 2 cut(s) 731, 957
DpnII GATC 2 cut(s) 729, 955
DraIII CACNNNGTG 1 cut(s) 574
EaeI YGGCCR 2 cut(s) 409, 754
Eam1104I CTCTTC 1 cut(s) 914
EarI CTCTTC 1 cut(s) 914
Eco130I CCWWGG 3 cut(s) 331, 412, 757
Eco147I AGGCCT 2 cut(s) 440, 880
Eco47I GGWCC 3 cut(s) 328, 544, 749
Eco57I CTGAAG 3 cut(s) 235, 1077, 1170
Eco88I CYCGRG 1 cut(s) 496
EcoO109I RGGNCCY 1 cut(s) 328
EcoRI GAATTC 1 cut(s) 399
EcoRII CCWGG 4 cut(s) 445, 709, 799, 1038
EcoT14I CCWWGG 3 cut(s) 331, 412, 757
EcoT22I ATGCAT 1 cut(s) 585
ErhI CCWWGG 3 cut(s) 331, 412, 757
FaeI CATG 9 cut(s) 18, 42, 64, 174, 264, 587, 761, 1161, 1169
FalI AAGNNNNNCTT 4 cut(s) 147, 179, 830, 862
FaqI GGGAC 3 cut(s) 188, 200, 238
FatI CATG 9 cut(s) 14, 38, 60, 170, 260, 583, 757, 1157, 1165
FauI CCCGC 2 cut(s) 215, 225
FbaI TGATCA 1 cut(s) 955
Fnu4HI GCNGC 2 cut(s) 19, 1182
FokI GGATG 5 cut(s) 301, 377, 617, 1022, 1130
Fsp4HI GCNGC 2 cut(s) 19, 1182
FspBI CTAG 5 cut(s) 236, 387, 779, 882, 897
GlaI GCGC 1 cut(s) 221
GluI GCNGC 2 cut(s) 19, 1182
HaeIII GGCC 8 cut(s) 259, 275, 375, 411, 440, 708, 756, 880
HapII CCGG 4 cut(s) 325, 468, 684, 753
HgaI GACGC 1 cut(s) 72
HhaI GCGC 1 cut(s) 222
Hin1II CATG 9 cut(s) 18, 42, 64, 174, 264, 587, 761, 1161, 1169
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HincII GTYRAC 3 cut(s) 115, 493, 721
HindII GTYRAC 3 cut(s) 115, 493, 721
HindIII AAGCTT 2 cut(s) 180, 1152
HinfI GANTC 3 cut(s) 146, 1005, 1090
HpaI GTTAAC 1 cut(s) 721
HpaII CCGG 4 cut(s) 325, 468, 684, 753
HphI GGTGA 4 cut(s) 435, 523, 649, 698
Hpy166II GTNNAC 8 cut(s) 44, 115, 211, 370, 493, 547, 721, 749
Hpy188I TCNGA 8 cut(s) 254, 295, 319, 560, 607, 652, 673, 1150
Hpy188III TCNNGA 6 cut(s) 61, 362, 404, 528, 859, 1002
Hpy8I GTNNAC 8 cut(s) 44, 115, 211, 370, 493, 547, 721, 749
Hpy99I CGWCG 1 cut(s) 1022
HpyAV CCTTC 3 cut(s) 67, 169, 290
HpyCH4III ACNGT 3 cut(s) 107, 718, 820
HpyCH4IV ACGT 4 cut(s) 213, 227, 1020, 1025
HpyF10VI GCNNNNNNNGC 1 cut(s) 805
HpyF3I CTNAG 1 cut(s) 118
HpySE526I ACGT 4 cut(s) 213, 227, 1020, 1025
Hsp92II CATG 9 cut(s) 18, 42, 64, 174, 264, 587, 761, 1161, 1169
HspAI GCGC 1 cut(s) 220
Ksp22I TGATCA 1 cut(s) 955
KspAI GTTAAC 1 cut(s) 721
Kzo9I GATC 2 cut(s) 729, 955
LmnI GCTCC 2 cut(s) 122, 470
Lsp1109I GCAGC 2 cut(s) 30, 1168
LweI GCATC 3 cut(s) 297, 639, 780
MaeI CTAG 5 cut(s) 236, 387, 779, 882, 897
MaeII ACGT 4 cut(s) 213, 227, 1020, 1025
MaeIII GTNAC 5 cut(s) 344, 566, 739, 814, 1021
MalI GATC 2 cut(s) 731, 957
MboI GATC 2 cut(s) 729, 955
MboII GAAGA 2 cut(s) 241, 901
MfeI CAATTG 2 cut(s) 539, 702
MhlI GDGCHC 1 cut(s) 372
MlsI TGGCCA 1 cut(s) 411
MluCI AATT 9 cut(s) 100, 399, 457, 539, 588, 702, 762, 1011, 1069
MluNI TGGCCA 1 cut(s) 411
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 2 cut(s) 751, 937
Mox20I TGGCCA 1 cut(s) 411
Mph1103I ATGCAT 1 cut(s) 585
MscI TGGCCA 1 cut(s) 411
MseI TTAA 5 cut(s) 96, 720, 822, 837, 864
MslI CAYNNNNRTG 3 cut(s) 509, 641, 1170
Msp20I TGGCCA 1 cut(s) 411
MspA1I CMGCKG 2 cut(s) 203, 1181
MspI CCGG 4 cut(s) 325, 468, 684, 753
MspR9I CCNGG 6 cut(s) 326, 447, 711, 753, 801, 1040
MunI CAATTG 2 cut(s) 539, 702
MvaI CCWGG 4 cut(s) 447, 711, 801, 1040
MvnI CGCG 2 cut(s) 220, 222
MwoI GCNNNNNNNGC 1 cut(s) 805
NciI CCSGG 2 cut(s) 326, 753
NcoI CCATGG 1 cut(s) 757
NdeII GATC 2 cut(s) 729, 955
NlaIII CATG 9 cut(s) 18, 42, 64, 174, 264, 587, 761, 1161, 1169
NlaIV GGNNCC 5 cut(s) 124, 301, 329, 715, 751
NmuCI GTSAC 2 cut(s) 566, 1021
NsiI ATGCAT 1 cut(s) 585
PaeR7I CTCGAG 1 cut(s) 496
PagI TCATGA 1 cut(s) 60
PceI AGGCCT 2 cut(s) 440, 880
PcsI WCGNNNNNNNCGW 1 cut(s) 1014
PfeI GAWTC 2 cut(s) 1005, 1090
PinAI ACCGGT 1 cut(s) 683
PkrI GCNGC 2 cut(s) 20, 1183
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
PpuMI RGGWCCY 1 cut(s) 328
PshBI ATTAAT 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 328
Psp6I CCWGG 4 cut(s) 445, 709, 799, 1038
PspGI CCWGG 4 cut(s) 445, 709, 799, 1038
PspN4I GGNNCC 5 cut(s) 124, 301, 329, 715, 751
PspPI GGNCC 5 cut(s) 257, 328, 544, 707, 749
PspPPI RGGWCCY 1 cut(s) 328
PspXI VCTCGAGB 1 cut(s) 496
PstI CTGCAG 1 cut(s) 1186
PstNI CAGNNNCTG 1 cut(s) 1055
PvuII CAGCTG 2 cut(s) 203, 1181
RsaI GTAC 4 cut(s) 45, 1028, 1045, 1099
RsaNI GTAC 4 cut(s) 44, 1027, 1044, 1098
RseI CAYNNNNRTG 3 cut(s) 509, 641, 1170
SaqAI TTAA 5 cut(s) 96, 720, 822, 837, 864
SatI GCNGC 2 cut(s) 19, 1182
Sau3AI GATC 2 cut(s) 729, 955
Sau96I GGNCC 5 cut(s) 257, 328, 544, 707, 749
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 6 cut(s) 326, 447, 711, 753, 801, 1040
SduI GDGCHC 1 cut(s) 372
SfaNI GCATC 3 cut(s) 297, 639, 780
SfcI CTRYAG 1 cut(s) 1182
Sfr274I CTCGAG 1 cut(s) 496
SinI GGWCC 3 cut(s) 328, 544, 749
SlaI CTCGAG 1 cut(s) 496
SmiMI CAYNNNNRTG 3 cut(s) 509, 641, 1170
SmlI CTYRAG 1 cut(s) 496
SmoI CTYRAG 1 cut(s) 496
Sse9I AATT 9 cut(s) 100, 399, 457, 539, 588, 702, 762, 1011, 1069
SseBI AGGCCT 2 cut(s) 440, 880
SsiI CCGC 2 cut(s) 218, 222
SspMI CTAG 5 cut(s) 236, 387, 779, 882, 897
StuI AGGCCT 2 cut(s) 440, 880
StyD4I CCNGG 6 cut(s) 324, 445, 709, 751, 799, 1038
StyI CCWWGG 3 cut(s) 331, 412, 757
TaaI ACNGT 3 cut(s) 107, 718, 820
TaiI ACGT 4 cut(s) 216, 230, 1023, 1028
TaqI TCGA 6 cut(s) 392, 497, 732, 930, 1008, 1074
TasI AATT 9 cut(s) 100, 399, 457, 539, 588, 702, 762, 1011, 1069
TatI WGTACW 1 cut(s) 43
TfiI GAWTC 2 cut(s) 1005, 1090
Tru1I TTAA 5 cut(s) 96, 720, 822, 837, 864
Tru9I TTAA 5 cut(s) 96, 720, 822, 837, 864
TscAI CASTG 5 cut(s) 94, 424, 486, 892, 1071
TseFI GTSAC 2 cut(s) 566, 1021
TseI GCWGC 2 cut(s) 18, 1181
Tsp45I GTSAC 2 cut(s) 566, 1021
TspDTI ATGAA 3 cut(s) 49, 77, 1146
TspGWI ACGGA 2 cut(s) 181, 411
TspRI CASTG 5 cut(s) 94, 424, 486, 892, 1071
VneI GTGCAC 1 cut(s) 368
VpaK11BI GGWCC 3 cut(s) 328, 544, 749
VspI ATTAAT 1 cut(s) 96
XapI RAATTY 2 cut(s) 399, 1069
XhoI CTCGAG 1 cut(s) 496
XspI CTAG 5 cut(s) 236, 387, 779, 882, 897
Zsp2I ATGCAT 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.