Prupe.6G261300_v2.0.a1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
N/A
Physical Location & Seq
Reverse (-)
25154777 .. 25156861
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G261300.1_v2.0.a1.CDS.2

Sequence Viewer

Length: 489 bp
ATGAAGGAGCTGATTGGGAGGCCTGGGTCGGTGAGTGGGTTATTGCTCAGGATAGGCCAATTTTCATGCGCTTCTATTGCCATTTCAGTCATGGTCACCACTGACGGCTTCGCTACCTTCACTGCTTTCTGCTATTTGATCGCATCAATGGGGCTTCAACTGCTATGGAGCTTGGGGCTTGCTTGTCTTGATATTTATGCTTTGTGGAAAAAGAGAGACCTTCAAAATCCAATCCTAGTGAGCCTATTTGTTGTAGGTAACAGCTACTCTATCACTAGCTGCTGCAAGCTCTTGCGCTGGGCTTACAGTTATGTTTTCAAGAGACCTACATTTCTGCAAGGGGGAATACCATCTCCCATGCATCAACTACAAGTTTGCAGTTGCGTTCACTTTTGTCACCTGGGGCCTTACTGCCGTATCTTCTCACGTGATGTTTTGGATCCTATTGGCATCGGTTTAGAGACTCATGTTTTTTTGCCCTACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

163

Amino Acids

18.05

Weight (kDa)

8.77

Isoelectric Point (pI)

52.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 434, 447
AcvI CACGTG 1 cut(s) 428
AgsI TTSAA 3 cut(s) 158, 224, 319
AjnI CCWGG 2 cut(s) 22, 399
AluBI AGCT 5 cut(s) 10, 171, 264, 279, 289
AluI AGCT 5 cut(s) 10, 171, 264, 279, 289
Alw26I GTCTC 3 cut(s) 210, 316, 455
AlwI GGATC 2 cut(s) 434, 447
AoxI GGCC 3 cut(s) 20, 55, 404
ApeKI GCWGC 2 cut(s) 279, 282
AspLEI GCGC 2 cut(s) 71, 297
AspS9I GGNCC 1 cut(s) 404
AsuHPI GGTGA 3 cut(s) 43, 88, 389
BaeI ACNNNNGTAYC 2 cut(s) 400, 433
BamHI GGATCC 1 cut(s) 439
BbrPI CACGTG 1 cut(s) 428
BbvI GCAGC 2 cut(s) 266, 269
BccI CCATC 1 cut(s) 358
BceAI ACGGC 2 cut(s) 121, 399
BciT130I CCWGG 2 cut(s) 24, 401
BcoDI GTCTC 3 cut(s) 210, 316, 455
BfaI CTAG 2 cut(s) 236, 276
BisI GCNGC 2 cut(s) 280, 283
BlsI GCNGC 2 cut(s) 281, 284
Bme1390I CCNGG 2 cut(s) 24, 401
BmgT120I GGNCC 1 cut(s) 404
BmiI GGNNCC 2 cut(s) 405, 441
BmrFI CCNGG 2 cut(s) 24, 401
BmsI GCATC 3 cut(s) 152, 370, 459
Bpu10I CCTNAGC 1 cut(s) 47
BsaAI YACGTR 1 cut(s) 428
BsaI GGTCTC 2 cut(s) 210, 316
BsaJI CCNNGG 2 cut(s) 23, 400
BseBI CCWGG 2 cut(s) 24, 401
BseDI CCNNGG 2 cut(s) 23, 400
BseMII CTCAG 1 cut(s) 61
BseXI GCAGC 2 cut(s) 266, 269
BseYI CCCAGC 1 cut(s) 297
BshFI GGCC 3 cut(s) 22, 57, 406
BsmAI GTCTC 3 cut(s) 210, 316, 455
BsnI GGCC 3 cut(s) 22, 57, 406
Bso31I GGTCTC 2 cut(s) 210, 316
Bsp143I GATC 2 cut(s) 138, 439
BspANI GGCC 3 cut(s) 22, 57, 406
BspCNI CTCAG 1 cut(s) 60
BspLI GGNNCC 2 cut(s) 405, 441
BspPI GGATC 2 cut(s) 434, 447
BspTNI GGTCTC 2 cut(s) 210, 316
BssECI CCNNGG 2 cut(s) 23, 400
BssMI GATC 2 cut(s) 138, 439
Bst2UI CCWGG 2 cut(s) 24, 401
Bst4CI ACNGT 1 cut(s) 308
BstBAI YACGTR 1 cut(s) 428
BstC8I GCNNGC 2 cut(s) 180, 287
BstDEI CTNAG 1 cut(s) 47
BstEII GGTNACC 1 cut(s) 94
BstHHI GCGC 2 cut(s) 71, 297
BstKTI GATC 2 cut(s) 141, 442
BstMAI GTCTC 3 cut(s) 210, 316, 455
BstMBI GATC 2 cut(s) 138, 439
BstMWI GCNNNNNNNGC 2 cut(s) 77, 160
BstNI CCWGG 2 cut(s) 24, 401
BstPI GGTNACC 1 cut(s) 94
BstSCI CCNGG 2 cut(s) 22, 399
BstV1I GCAGC 2 cut(s) 266, 269
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
BsuRI GGCC 3 cut(s) 22, 57, 406
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 2 cut(s) 99, 120
Cac8I GCNNGC 2 cut(s) 180, 287
CfoI GCGC 2 cut(s) 71, 297
Cfr13I GGNCC 1 cut(s) 404
CviAII CATG 4 cut(s) 66, 91, 358, 467
DdeI CTNAG 1 cut(s) 47
DpnI GATC 2 cut(s) 140, 441
DpnII GATC 2 cut(s) 138, 439
Eco147I AGGCCT 1 cut(s) 22
Eco31I GGTCTC 2 cut(s) 210, 316
Eco72I CACGTG 1 cut(s) 428
Eco91I GGTNACC 1 cut(s) 94
EcoO109I RGGNCCY 1 cut(s) 404
EcoO65I GGTNACC 1 cut(s) 94
EcoRII CCWGG 2 cut(s) 22, 399
EcoT22I ATGCAT 1 cut(s) 363
FaeI CATG 4 cut(s) 69, 94, 361, 470
FaiI YATR 7 cut(s) 67, 92, 166, 198, 312, 359, 468
FatI CATG 4 cut(s) 65, 90, 357, 466
Fnu4HI GCNGC 2 cut(s) 280, 283
Fsp4HI GCNGC 2 cut(s) 280, 283
FspBI CTAG 2 cut(s) 236, 276
GlaI GCGC 2 cut(s) 70, 296
GluI GCNGC 2 cut(s) 280, 283
GsaI CCCAGC 1 cut(s) 301
HaeIII GGCC 3 cut(s) 22, 57, 406
HhaI GCGC 2 cut(s) 71, 297
Hin1II CATG 4 cut(s) 69, 94, 361, 470
Hin6I GCGC 2 cut(s) 69, 295
HinP1I GCGC 2 cut(s) 69, 295
HinfI GANTC 1 cut(s) 463
HphI GGTGA 3 cut(s) 43, 88, 389
Hpy166II GTNNAC 1 cut(s) 388
Hpy188III TCNNGA 3 cut(s) 49, 188, 319
Hpy8I GTNNAC 1 cut(s) 388
HpyAV CCTTC 2 cut(s) 127, 230
HpyCH4III ACNGT 1 cut(s) 308
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 4 cut(s) 285, 337, 361, 378
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 160
HpyF3I CTNAG 1 cut(s) 47
HpySE526I ACGT 1 cut(s) 427
Hsp92II CATG 4 cut(s) 69, 94, 361, 470
HspAI GCGC 2 cut(s) 69, 295
Kzo9I GATC 2 cut(s) 138, 439
LmnI GCTCC 2 cut(s) 7, 168
LpnPI CCDG 6 cut(s) 9, 34, 36, 283, 386, 413
Lsp1109I GCAGC 2 cut(s) 266, 269
LweI GCATC 3 cut(s) 152, 370, 459
MaeI CTAG 2 cut(s) 236, 276
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 3 cut(s) 94, 257, 395
MalI GATC 2 cut(s) 140, 441
MboI GATC 2 cut(s) 138, 439
MboII GAAGA 1 cut(s) 412
MflI RGATCY 1 cut(s) 439
MluCI AATT 1 cut(s) 59
MlyI GAGTC 1 cut(s) 457
MnlI CCTC 1 cut(s) 12
Mph1103I ATGCAT 1 cut(s) 363
MspR9I CCNGG 2 cut(s) 24, 401
MvaI CCWGG 2 cut(s) 24, 401
MwoI GCNNNNNNNGC 2 cut(s) 77, 160
NdeII GATC 2 cut(s) 138, 439
NlaIII CATG 4 cut(s) 69, 94, 361, 470
NlaIV GGNNCC 2 cut(s) 405, 441
NmuCI GTSAC 2 cut(s) 94, 395
NsiI ATGCAT 1 cut(s) 363
PceI AGGCCT 1 cut(s) 22
PkrI GCNGC 2 cut(s) 281, 284
PleI GAGTC 1 cut(s) 457
PmaCI CACGTG 1 cut(s) 428
PmlI CACGTG 1 cut(s) 428
PpsI GAGTC 1 cut(s) 457
Ppu21I YACGTR 1 cut(s) 428
Psp6I CCWGG 2 cut(s) 22, 399
PspCI CACGTG 1 cut(s) 428
PspEI GGTNACC 1 cut(s) 94
PspFI CCCAGC 1 cut(s) 297
PspGI CCWGG 2 cut(s) 22, 399
PspN4I GGNNCC 2 cut(s) 405, 441
PspPI GGNCC 1 cut(s) 404
PsuI RGATCY 1 cut(s) 439
SatI GCNGC 2 cut(s) 280, 283
Sau3AI GATC 2 cut(s) 138, 439
Sau96I GGNCC 1 cut(s) 404
SchI GAGTC 1 cut(s) 457
ScrFI CCNGG 2 cut(s) 24, 401
SfaNI GCATC 3 cut(s) 152, 370, 459
Sse9I AATT 1 cut(s) 59
SseBI AGGCCT 1 cut(s) 22
SspMI CTAG 2 cut(s) 236, 276
StuI AGGCCT 1 cut(s) 22
StyD4I CCNGG 2 cut(s) 22, 399
TaaI ACNGT 1 cut(s) 308
TaiI ACGT 1 cut(s) 430
TasI AATT 1 cut(s) 59
TscAI CASTG 2 cut(s) 106, 127
TseFI GTSAC 2 cut(s) 94, 395
TseI GCWGC 2 cut(s) 279, 282
Tsp45I GTSAC 2 cut(s) 94, 395
TspDTI ATGAA 2 cut(s) 17, 54
TspRI CASTG 2 cut(s) 106, 127
XcmI CCANNNNNNNNNTGG 1 cut(s) 88
XspI CTAG 2 cut(s) 236, 276
Zsp2I ATGCAT 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.