Prupe.6G333300_v2.0.a1

lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
N/A
Physical Location & Seq
Forward (+)
28988996 .. 28992503
3508 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G333300.1_v2.0.a1.CDS.1

Sequence Viewer

Length: 1239 bp
ATGGAGAAAACAAAATCATCCCTTCATTCTCCCACTAACGGAGGTCTCACTACTATTCTTAGTATCGATCGAGGTGGAATAACAGGGATTATCGAAGGAGTTTCACTCAAGTTCCTGGAGTATGAGCTTCAGAGGCTTGATGGTAAGCATGCAAGGCTCGTAGATTATTTTGATTGGGTTGCAGGGACGAGCACAGGTGGCCTTGTGATGCTCATGCTTGCTACCCCAGATGAAAATAACCGTCCTTTGTTTGCTGCAAAAGATATTCTCCCTTTTTATCTCAAACATTGCCCTAAAATCTTCCACCAACCTAGATCTCTCATCGGCAAAATCAAACATTGTTTGAAAGGTTTAGTAAGATCCAAATATAATGGGAAGTATTTCCACAAATTGGTGAAGGAAAAACTAGGCGATAAGCATTTGCACGACACATTGACTAATGTTGTAATCCCAACAGTTGATATAAAACTGCCATGTCCAGTTATTTTCTCCAGCTATGAGTTGAAAAGGCACCCAAGCCTAGATGCTTTGCTGTCGGAGATATGCATTGGAACTTCAGCAGCCCCACCCTACTTTCCGGCCCATCAATTTAGTTCCACAACTTCAAGTCAAGAGAATTCAATCTCATTGATGGCTTCAATTGCTATAAAGGAAGCGGAAAAAGAAATCCTCAAGAGGAATCCTGGGTGTGGAAATGAAGAGTTGCATAAGCGGATTTTACTAATATCACTGGGGACTGGTATAGCAAATGATGAGGAGGAGTACGACGCCAAAGAAGTAGCTCGTTGGGGTGCTTTCCAGTGGCTCATCGGACCCCATTGGTCGTCTCCAGTGGTGCATATGTGTATCAAAGTAACTGGTGGCATTCTTGATTACCACACTCAATGCGTTTTAGAAGATGTTCAGTCTCACGGCCATTACCTCCGGATTCAGGACGATACACTAAGCGGAAGATTGGCTTCTGTGGATATTGCCACACAAAAAAACTTGAATGATCTTGTGAAAGTTGGAGAGGCATTGTTGAAGAAACCGGTTTCCAGATTGGATTTCGATACCAGTGAAACCGAGCCTCTTCCTCTTGAGACAAATGCAGAGGCTCTCGTGAGGATGGCGAAAATTCTCTCTGAGGAGAGGGCTCAGAGGATTCAGGAAGGACATCATCCTCGGAGAATTCAGGAAGGACATCATCACAAAAATAATAGATTAGATGCTCTCACCAAAGCATCTTTTGTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

413

Amino Acids

46.01

Weight (kDa)

7.72

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 510
AccB7I CCANNNNNTGG 1 cut(s) 391
AccIII TCCGGA 1 cut(s) 924
AciI CCGC 3 cut(s) 656, 712, 948
AclWI GGATC 1 cut(s) 354
AcoI YGGCCR 1 cut(s) 913
AcsI RAATTY 3 cut(s) 616, 1116, 1170
AcuI CTGAAG 2 cut(s) 113, 540
AcyI GRCGYC 1 cut(s) 768
AfaI GTAC 1 cut(s) 764
AfiI CCNNNNNNNGG 4 cut(s) 38, 391, 689, 931
AgeI ACCGGT 1 cut(s) 1030
AgsI TTSAA 7 cut(s) 346, 505, 606, 621, 639, 991, 1024
AjnI CCWGG 2 cut(s) 114, 682
AluBI AGCT 3 cut(s) 127, 495, 782
AluI AGCT 3 cut(s) 127, 495, 782
Alw21I GWGCWC 1 cut(s) 194
Alw26I GTCTC 4 cut(s) 50, 831, 912, 1076
AlwI GGATC 1 cut(s) 354
Aor13HI TCCGGA 1 cut(s) 924
AoxI GGCC 3 cut(s) 199, 579, 913
ApeKI GCWGC 2 cut(s) 254, 560
ApoI RAATTY 3 cut(s) 616, 1116, 1170
AsiGI ACCGGT 1 cut(s) 1030
Asp700I GAANNNNTTC 2 cut(s) 380, 900
AspS9I GGNCC 2 cut(s) 580, 812
AsuHPI GGTGA 2 cut(s) 406, 1207
AvaII GGWCC 1 cut(s) 812
BanI GGYRCC 1 cut(s) 510
BanII GRGCYC 1 cut(s) 1138
BauI CACGAG 1 cut(s) 1100
Bbv12I GWGCWC 1 cut(s) 194
BbvI GCAGC 2 cut(s) 241, 572
BccI CCATC 4 cut(s) 134, 591, 625, 1102
BceAI ACGGC 1 cut(s) 928
BciT130I CCWGG 2 cut(s) 116, 684
BcoDI GTCTC 4 cut(s) 50, 831, 912, 1076
BfaI CTAG 3 cut(s) 312, 407, 521
BglII AGATCT 1 cut(s) 314
BisI GCNGC 2 cut(s) 255, 561
BlsI GCNGC 2 cut(s) 256, 562
Bme1390I CCNGG 2 cut(s) 116, 684
Bme18I GGWCC 1 cut(s) 812
BmgT120I GGNCC 2 cut(s) 580, 812
BmiI GGNNCC 2 cut(s) 512, 814
BmrFI CCNGG 2 cut(s) 116, 684
BmrI ACTGGG 1 cut(s) 740
BmsI GCATC 4 cut(s) 198, 514, 1198, 1232
BmuI ACTGGG 1 cut(s) 740
BplI GAGNNNNNCTC 2 cut(s) 90, 122
BpmI CTGGAG 3 cut(s) 137, 475, 813
BpuEI CTTGAG 3 cut(s) 92, 656, 1100
Bsa29I ATCGAT 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 768
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 683, 1163
BsaWI WCCGGW 2 cut(s) 924, 1030
Bsc4I CCNNNNNNNGG 4 cut(s) 38, 391, 689, 931
Bse118I RCCGGY 1 cut(s) 1030
Bse1I ACTGG 7 cut(s) 479, 735, 742, 799, 830, 862, 1056
Bse3DI GCAATG 1 cut(s) 286
BseAI TCCGGA 1 cut(s) 924
BseBI CCWGG 2 cut(s) 116, 684
BseCI ATCGAT 1 cut(s) 66
BseDI CCNNGG 2 cut(s) 683, 1163
BseGI GGATG 3 cut(s) 17, 1113, 1159
BseLI CCNNNNNNNGG 4 cut(s) 38, 391, 689, 931
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 2 cut(s) 1116, 1151
BseNI ACTGG 7 cut(s) 479, 735, 742, 799, 830, 862, 1056
BseRI GAGGAG 3 cut(s) 770, 773, 1142
BseXI GCAGC 2 cut(s) 241, 572
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 3 cut(s) 201, 581, 915
BshNI GGYRCC 1 cut(s) 510
BshTI ACCGGT 1 cut(s) 1030
BshVI ATCGAT 1 cut(s) 66
BsiEI CGRYCG 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 194
BsiSI CCGG 3 cut(s) 578, 925, 1031
BslFI GGGAC 2 cut(s) 199, 748
BslI CCNNNNNNNGG 4 cut(s) 38, 391, 689, 931
BsmAI GTCTC 4 cut(s) 50, 831, 912, 1076
BsmBI CGTCTC 1 cut(s) 831
BsmFI GGGAC 2 cut(s) 199, 748
BsmI GAATGC 1 cut(s) 864
BsnI GGCC 3 cut(s) 201, 581, 915
Bso31I GGTCTC 1 cut(s) 50
Bsp1286I GDGCHC 2 cut(s) 194, 1138
Bsp13I TCCGGA 1 cut(s) 924
Bsp143I GATC 4 cut(s) 67, 314, 359, 994
BspACI CCGC 3 cut(s) 656, 712, 948
BspANI GGCC 3 cut(s) 201, 581, 915
BspCNI CTCAG 2 cut(s) 1117, 1150
BspDI ATCGAT 1 cut(s) 66
BspEI TCCGGA 1 cut(s) 924
BspLI GGNNCC 2 cut(s) 512, 814
BspPI GGATC 1 cut(s) 354
BspT107I GGYRCC 1 cut(s) 510
BspTNI GGTCTC 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 286
BsrFI RCCGGY 1 cut(s) 1030
BsrI ACTGG 7 cut(s) 479, 735, 742, 799, 830, 862, 1056
BssAI RCCGGY 1 cut(s) 1030
BssECI CCNNGG 2 cut(s) 683, 1163
BssMI GATC 4 cut(s) 67, 314, 359, 994
BssNI GRCGYC 1 cut(s) 768
BssSI CACGAG 1 cut(s) 1100
Bst2BI CACGAG 1 cut(s) 1100
Bst2UI CCWGG 2 cut(s) 116, 684
Bst4CI ACNGT 2 cut(s) 242, 457
Bst6I CTCTTC 2 cut(s) 693, 1077
BstACI GRCGYC 1 cut(s) 768
BstC8I GCNNGC 2 cut(s) 150, 219
BstDEI CTNAG 4 cut(s) 59, 944, 1125, 1137
BstF5I GGATG 3 cut(s) 17, 1113, 1159
BstKTI GATC 4 cut(s) 70, 317, 362, 997
BstMAI GTCTC 4 cut(s) 50, 831, 912, 1076
BstMBI GATC 4 cut(s) 67, 314, 359, 994
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 4 cut(s) 133, 154, 198, 641
BstNI CCWGG 2 cut(s) 116, 684
BstNSI RCATGY 1 cut(s) 152
BstSCI CCNGG 2 cut(s) 114, 682
BstV1I GCAGC 2 cut(s) 241, 572
BstX2I RGATCY 2 cut(s) 314, 359
BstYI RGATCY 2 cut(s) 314, 359
Bsu15I ATCGAT 1 cut(s) 66
BsuRI GGCC 3 cut(s) 201, 581, 915
BsuTUI ATCGAT 1 cut(s) 66
BtsCI GGATG 3 cut(s) 17, 1113, 1159
BtsIMutI CAGTG 4 cut(s) 728, 806, 837, 1063
Cac8I GCNNGC 2 cut(s) 150, 219
Cfr10I RCCGGY 1 cut(s) 1030
Cfr13I GGNCC 2 cut(s) 580, 812
ClaI ATCGAT 1 cut(s) 66
CseI GACGC 1 cut(s) 776
Csp6I GTAC 1 cut(s) 763
CspAI ACCGGT 1 cut(s) 1030
CviAII CATG 3 cut(s) 149, 214, 474
CviQI GTAC 1 cut(s) 763
DdeI CTNAG 4 cut(s) 59, 944, 1125, 1137
DpnI GATC 4 cut(s) 69, 316, 361, 996
DpnII GATC 4 cut(s) 67, 314, 359, 994
EaeI YGGCCR 1 cut(s) 913
Eam1104I CTCTTC 2 cut(s) 693, 1077
EarI CTCTTC 2 cut(s) 693, 1077
Eco24I GRGCYC 1 cut(s) 1138
Eco31I GGTCTC 1 cut(s) 50
Eco47I GGWCC 1 cut(s) 812
Eco57I CTGAAG 2 cut(s) 113, 540
EcoRI GAATTC 2 cut(s) 616, 1170
EcoRII CCWGG 2 cut(s) 114, 682
EcoT22I ATGCAT 1 cut(s) 548
EcoT38I GRGCYC 1 cut(s) 1138
Esp3I CGTCTC 1 cut(s) 831
FaeI CATG 3 cut(s) 152, 217, 477
FalI AAGNNNNNCTT 2 cut(s) 943, 975
FaqI GGGAC 2 cut(s) 199, 748
FatI CATG 3 cut(s) 148, 213, 473
FauNDI CATATG 1 cut(s) 840
Fnu4HI GCNGC 2 cut(s) 255, 561
FokI GGATG 3 cut(s) 4, 1120, 1146
FriOI GRGCYC 1 cut(s) 1138
Fsp4HI GCNGC 2 cut(s) 255, 561
FspBI CTAG 3 cut(s) 312, 407, 521
GluI GCNGC 2 cut(s) 255, 561
GsuI CTGGAG 3 cut(s) 137, 475, 813
HaeIII GGCC 3 cut(s) 201, 581, 915
HapII CCGG 3 cut(s) 578, 925, 1031
HgaI GACGC 1 cut(s) 776
Hin1I GRCGYC 1 cut(s) 768
Hin1II CATG 3 cut(s) 152, 217, 477
HinfI GANTC 3 cut(s) 679, 928, 1144
HpaII CCGG 3 cut(s) 578, 925, 1031
HphI GGTGA 2 cut(s) 406, 1207
Hpy188I TCNGA 6 cut(s) 132, 538, 812, 1126, 1140, 1167
Hpy99I CGWCG 1 cut(s) 770
HpyAV CCTTC 5 cut(s) 32, 89, 391, 1145, 1172
HpyCH4III ACNGT 2 cut(s) 242, 457
HpyCH4V TGCA 8 cut(s) 152, 182, 257, 424, 546, 706, 838, 1091
HpyF10VI GCNNNNNNNGC 4 cut(s) 133, 154, 198, 641
HpyF3I CTNAG 4 cut(s) 59, 944, 1125, 1137
Hsp92I GRCGYC 1 cut(s) 768
Hsp92II CATG 3 cut(s) 152, 217, 477
Kpn2I TCCGGA 1 cut(s) 924
Kzo9I GATC 4 cut(s) 67, 314, 359, 994
Lsp1109I GCAGC 2 cut(s) 241, 572
LweI GCATC 4 cut(s) 198, 514, 1198, 1232
MaeI CTAG 3 cut(s) 312, 407, 521
MaeIII GTNAC 1 cut(s) 853
MalI GATC 4 cut(s) 69, 316, 361, 996
MboI GATC 4 cut(s) 67, 314, 359, 994
MboII GAAGA 6 cut(s) 292, 710, 908, 963, 1036, 1064
MfeI CAATTG 1 cut(s) 639
MflI RGATCY 2 cut(s) 314, 359
MhlI GDGCHC 2 cut(s) 194, 1138
MluCI AATT 6 cut(s) 389, 587, 616, 639, 1116, 1170
MmeI TCCRAC 2 cut(s) 516, 988
Mph1103I ATGCAT 1 cut(s) 548
MroI TCCGGA 1 cut(s) 924
MroXI GAANNNNTTC 2 cut(s) 380, 900
MspI CCGG 3 cut(s) 578, 925, 1031
MspR9I CCNGG 2 cut(s) 116, 684
MunI CAATTG 1 cut(s) 639
Mva1269I GAATGC 1 cut(s) 864
MvaI CCWGG 2 cut(s) 116, 684
MwoI GCNNNNNNNGC 4 cut(s) 133, 154, 198, 641
NdeI CATATG 1 cut(s) 840
NdeII GATC 4 cut(s) 67, 314, 359, 994
NlaIII CATG 3 cut(s) 152, 217, 477
NlaIV GGNNCC 2 cut(s) 512, 814
NsiI ATGCAT 1 cut(s) 548
NspI RCATGY 1 cut(s) 152
PaeI GCATGC 1 cut(s) 152
PctI GAATGC 1 cut(s) 864
PdmI GAANNNNTTC 2 cut(s) 380, 900
PfeI GAWTC 3 cut(s) 679, 928, 1144
PflMI CCANNNNNTGG 1 cut(s) 391
PfoI TCCNGGA 1 cut(s) 114
PinAI ACCGGT 1 cut(s) 1030
PkrI GCNGC 2 cut(s) 256, 562
Ple19I CGATCG 1 cut(s) 70
Psp6I CCWGG 2 cut(s) 114, 682
PspGI CCWGG 2 cut(s) 114, 682
PspN4I GGNNCC 2 cut(s) 512, 814
PspPI GGNCC 2 cut(s) 580, 812
PsuI RGATCY 2 cut(s) 314, 359
PvuI CGATCG 1 cut(s) 70
RsaI GTAC 1 cut(s) 764
RsaNI GTAC 1 cut(s) 763
SatI GCNGC 2 cut(s) 255, 561
Sau3AI GATC 4 cut(s) 67, 314, 359, 994
Sau96I GGNCC 2 cut(s) 580, 812
ScrFI CCNGG 2 cut(s) 116, 684
SduI GDGCHC 2 cut(s) 194, 1138
SetI ASST 9 cut(s) 46, 76, 129, 199, 313, 352, 497, 784, 924
SfaNI GCATC 4 cut(s) 198, 514, 1198, 1232
SinI GGWCC 1 cut(s) 812
SmlI CTYRAG 3 cut(s) 107, 671, 1079
SmoI CTYRAG 3 cut(s) 107, 671, 1079
SphI GCATGC 1 cut(s) 152
Sse9I AATT 6 cut(s) 389, 587, 616, 639, 1116, 1170
SsiI CCGC 3 cut(s) 656, 712, 948
SspMI CTAG 3 cut(s) 312, 407, 521
StyD4I CCNGG 2 cut(s) 114, 682
TaaI ACNGT 2 cut(s) 242, 457
TaqI TCGA 4 cut(s) 66, 70, 93, 1050
TasI AATT 6 cut(s) 389, 587, 616, 639, 1116, 1170
TfiI GAWTC 3 cut(s) 679, 928, 1144
TscAI CASTG 4 cut(s) 735, 806, 837, 1063
TseI GCWGC 2 cut(s) 254, 560
TspDTI ATGAA 3 cut(s) 14, 246, 711
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 4 cut(s) 735, 806, 837, 1063
Van91I CCANNNNNTGG 1 cut(s) 391
VpaK11BI GGWCC 1 cut(s) 812
XapI RAATTY 3 cut(s) 616, 1116, 1170
XceI RCATGY 1 cut(s) 152
XmnI GAANNNNTTC 2 cut(s) 380, 900
XspI CTAG 3 cut(s) 312, 407, 521
Zsp2I ATGCAT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.