Prupe.7G036100_v2.0.a1

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
N/A
Physical Location & Seq
Forward (+)
6624197 .. 6626309
2113 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G036100.1_v2.0.a1.CDS.4

Sequence Viewer

Length: 924 bp
ATGTATGGACTGCAGCATGTACCAGAAGGGAGATATTTGCTTCGCAATGCTATAGTTTTCAAAGGTGATAACTGCAAAAGCACAGATATTACTTTTCGAATTGATGGAACATTGATAGCTTCGTCTGATTACCGGATCCTTGGCCAATCTTTCAACTGGCTAAGCTTTGAAGGCGTTACCGGTGTGTCCATTATCGGAGGTGCCCTTGATGCTAAAGGTACCGCCTTGTGGGCATGCAAATTGGCTGGAAGGACTGATTGCCCCAACGGAGGAGCCACGAGTCTGAGTTTTACAAACTCCAAAAACATTAACTTCAATGGACTAATGTCATTGAATAGCCAGATGTTCCACATTGCTCGCATCAAAGGGGTCAAAGTCAATGCAGCTGGAAATAGCCCAAACACTGATGGCATCCATGTCCAATTATCAAGAAATGTTGCAATCTTCAACACCTCTATCAAAACTGGAGACAATTGTGTCTCGATTGGCCCTGGAAGAAAGGACTTGTGGATCGAGCAAATAACTTGTGGTCCTGGCCATGACATTAGTTGTTCAGGGTACTCAAAATGGTTTGAGGATTGGGCGAGGCCGAGCAACGGATTTGTTCAGGGTGTTCAATTCCTTGATGTCGTAATGATTAACGTCCAAAATCCTATTGTAATAGACCAAAACTATTGCCCACACAACATCAACTGCCCTGCTCAGGTATCAGGTGTGAAAGTCAGTGATGTCATATGCCGGAACATTCAAGGATTATCAGCTACAATCGTCGCAATAAATTTTGATTGTAGCGCAACAAATCCATGTAGTGGGATAAGACTCGAAAACGTGAGTTTGACCTGTAGGAATCAAGAGCTTCAGTCACATTGTGCCAATGCAATTGGCAAGATTGTTGGCACTGTTCAACCAAATAGTTGTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

308

Amino Acids

33.04

Weight (kDa)

8.18

Isoelectric Point (pI)

30.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 476
Acc65I GGTACC 1 cut(s) 218
AccB1I GGYRCC 2 cut(s) 200, 218
AccB7I CCANNNNNTGG 1 cut(s) 809
AciI CCGC 1 cut(s) 222
AclWI GGATC 3 cut(s) 130, 143, 518
AcoI YGGCCR 2 cut(s) 142, 535
AcsI RAATTY 1 cut(s) 778
AcuI CTGAAG 1 cut(s) 842
AdeI CACNNNGTG 1 cut(s) 869
AfaI GTAC 3 cut(s) 21, 220, 560
AfiI CCNNNNNNNGG 5 cut(s) 228, 269, 596, 703, 809
AgeI ACCGGT 1 cut(s) 179
AgsI TTSAA 9 cut(s) 61, 154, 170, 316, 334, 448, 617, 749, 905
AjnI CCWGG 2 cut(s) 490, 532
AluBI AGCT 5 cut(s) 119, 165, 386, 761, 856
AluI AGCT 5 cut(s) 119, 165, 386, 761, 856
Alw26I GTCTC 2 cut(s) 462, 484
AlwI GGATC 3 cut(s) 130, 143, 518
AoxI GGCC 4 cut(s) 142, 487, 535, 587
ApeKI GCWGC 2 cut(s) 13, 383
ApoI RAATTY 1 cut(s) 778
AsiGI ACCGGT 1 cut(s) 179
Asp718I GGTACC 1 cut(s) 218
AspLEI GCGC 1 cut(s) 794
AspS9I GGNCC 2 cut(s) 488, 530
AsuHPI GGTGA 1 cut(s) 77
AsuII TTCGAA 1 cut(s) 97
AvaII GGWCC 1 cut(s) 530
BaeGI GKGCMC 1 cut(s) 205
BalI TGGCCA 2 cut(s) 144, 537
BamHI GGATCC 1 cut(s) 135
BanI GGYRCC 2 cut(s) 200, 218
BauI CACGAG 1 cut(s) 277
BbvI GCAGC 2 cut(s) 25, 395
BccI CCATC 2 cut(s) 98, 401
BciT130I CCWGG 2 cut(s) 492, 534
BcoDI GTCTC 2 cut(s) 462, 484
BfmI CTRYAG 3 cut(s) 11, 51, 841
BglI GCCNNNNNGGC 1 cut(s) 230
BisI GCNGC 2 cut(s) 14, 384
BlpI GCTNAGC 1 cut(s) 161
BlsI GCNGC 2 cut(s) 15, 385
Bme1390I CCNGG 2 cut(s) 492, 534
Bme18I GGWCC 1 cut(s) 530
BmgT120I GGNCC 2 cut(s) 488, 530
BmiI GGNNCC 4 cut(s) 137, 202, 220, 274
BmrFI CCNGG 2 cut(s) 492, 534
BmsI GCATC 3 cut(s) 199, 369, 420
BoxI GACNNNNGTC 1 cut(s) 325
BpmI CTGGAG 1 cut(s) 486
Bpu10I CCTNAGC 1 cut(s) 702
Bpu1102I GCTNAGC 1 cut(s) 161
Bpu14I TTCGAA 1 cut(s) 97
BsaJI CCNNGG 2 cut(s) 139, 490
BsaWI WCCGGW 2 cut(s) 132, 179
Bsc4I CCNNNNNNNGG 5 cut(s) 228, 269, 596, 703, 809
Bse118I RCCGGY 1 cut(s) 179
Bse1I ACTGG 2 cut(s) 161, 469
Bse3DI GCAATG 2 cut(s) 52, 351
BseBI CCWGG 2 cut(s) 492, 534
BseDI CCNNGG 2 cut(s) 139, 490
BseGI GGATG 1 cut(s) 411
BseLI CCNNNNNNNGG 5 cut(s) 228, 269, 596, 703, 809
BseMI GCAATG 2 cut(s) 52, 351
BseMII CTCAG 2 cut(s) 275, 716
BseNI ACTGG 2 cut(s) 161, 469
BseRI GAGGAG 1 cut(s) 285
BseSI GKGCMC 1 cut(s) 205
BseXI GCAGC 2 cut(s) 25, 395
BshFI GGCC 4 cut(s) 144, 489, 537, 589
BshNI GGYRCC 2 cut(s) 200, 218
BshTI ACCGGT 1 cut(s) 179
BsiSI CCGG 3 cut(s) 133, 180, 739
BslI CCNNNNNNNGG 5 cut(s) 228, 269, 596, 703, 809
BsmAI GTCTC 2 cut(s) 462, 484
BsnI GGCC 4 cut(s) 144, 489, 537, 589
Bsp119I TTCGAA 1 cut(s) 97
Bsp1286I GDGCHC 1 cut(s) 205
Bsp143I GATC 2 cut(s) 135, 510
Bsp1720I GCTNAGC 1 cut(s) 161
BspACI CCGC 1 cut(s) 222
BspANI GGCC 4 cut(s) 144, 489, 537, 589
BspCNI CTCAG 2 cut(s) 276, 715
BspLI GGNNCC 4 cut(s) 137, 202, 220, 274
BspMAI CTGCAG 1 cut(s) 15
BspPI GGATC 3 cut(s) 130, 143, 518
BspT104I TTCGAA 1 cut(s) 97
BspT107I GGYRCC 2 cut(s) 200, 218
BsrDI GCAATG 2 cut(s) 52, 351
BsrFI RCCGGY 1 cut(s) 179
BsrI ACTGG 2 cut(s) 161, 469
BssAI RCCGGY 1 cut(s) 179
BssECI CCNNGG 2 cut(s) 139, 490
BssMI GATC 2 cut(s) 135, 510
BssSI CACGAG 1 cut(s) 277
BssT1I CCWWGG 1 cut(s) 139
Bst2BI CACGAG 1 cut(s) 277
Bst2UI CCWGG 2 cut(s) 492, 534
Bst4CI ACNGT 1 cut(s) 901
BstBI TTCGAA 1 cut(s) 97
BstC8I GCNNGC 2 cut(s) 235, 358
BstDEI CTNAG 3 cut(s) 161, 284, 702
BstF5I GGATG 1 cut(s) 411
BstHHI GCGC 1 cut(s) 794
BstKTI GATC 2 cut(s) 138, 513
BstMAI GTCTC 2 cut(s) 462, 484
BstMBI GATC 2 cut(s) 135, 510
BstMWI GCNNNNNNNGC 3 cut(s) 171, 209, 230
BstNI CCWGG 2 cut(s) 492, 534
BstNSI RCATGY 2 cut(s) 20, 237
BstPAI GACNNNNGTC 1 cut(s) 325
BstSCI CCNGG 2 cut(s) 490, 532
BstSFI CTRYAG 3 cut(s) 11, 51, 841
BstSLI GKGCMC 1 cut(s) 205
BstV1I GCAGC 2 cut(s) 25, 395
BstX2I RGATCY 1 cut(s) 135
BstYI RGATCY 1 cut(s) 135
BsuRI GGCC 4 cut(s) 144, 489, 537, 589
BtsCI GGATG 1 cut(s) 411
BtsIMutI CAGTG 3 cut(s) 402, 730, 897
Cac8I GCNNGC 2 cut(s) 235, 358
CfoI GCGC 1 cut(s) 794
Cfr10I RCCGGY 1 cut(s) 179
Cfr13I GGNCC 2 cut(s) 488, 530
Csp6I GTAC 3 cut(s) 20, 219, 559
CspAI ACCGGT 1 cut(s) 179
CviAII CATG 5 cut(s) 17, 234, 416, 539, 804
CviQI GTAC 3 cut(s) 20, 219, 559
DdeI CTNAG 3 cut(s) 161, 284, 702
DpnI GATC 2 cut(s) 137, 512
DpnII GATC 2 cut(s) 135, 510
DraIII CACNNNGTG 1 cut(s) 869
DrdI GACNNNNNNGTC 1 cut(s) 476
DseDI GACNNNNNNGTC 1 cut(s) 476
EaeI YGGCCR 2 cut(s) 142, 535
Eco130I CCWWGG 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 530
Eco57I CTGAAG 1 cut(s) 842
EcoRII CCWGG 2 cut(s) 490, 532
EcoT14I CCWWGG 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 5 cut(s) 20, 237, 419, 542, 807
FaiI YATR 9 cut(s) 6, 18, 53, 235, 417, 540, 734, 736, 805
FatI CATG 5 cut(s) 16, 233, 415, 538, 803
FauNDI CATATG 1 cut(s) 734
Fnu4HI GCNGC 2 cut(s) 14, 384
FokI GGATG 1 cut(s) 398
Fsp4HI GCNGC 2 cut(s) 14, 384
GlaI GCGC 1 cut(s) 793
GluI GCNGC 2 cut(s) 14, 384
GsuI CTGGAG 1 cut(s) 486
HaeIII GGCC 4 cut(s) 144, 489, 537, 589
HapII CCGG 3 cut(s) 133, 180, 739
HhaI GCGC 1 cut(s) 794
Hin1II CATG 5 cut(s) 20, 237, 419, 542, 807
Hin6I GCGC 1 cut(s) 792
HinP1I GCGC 1 cut(s) 792
HindIII AAGCTT 1 cut(s) 163
HinfI GANTC 3 cut(s) 280, 819, 847
HpaII CCGG 3 cut(s) 133, 180, 739
HphI GGTGA 1 cut(s) 77
Hpy188I TCNGA 3 cut(s) 127, 197, 285
Hpy188III TCNNGA 3 cut(s) 429, 481, 851
Hpy99I CGWCG 1 cut(s) 773
HpyAV CCTTC 3 cut(s) 20, 164, 243
HpyCH4III ACNGT 1 cut(s) 901
HpyCH4IV ACGT 2 cut(s) 642, 828
HpyCH4V TGCA 6 cut(s) 13, 75, 237, 383, 440, 878
HpyF10VI GCNNNNNNNGC 3 cut(s) 171, 209, 230
HpyF3I CTNAG 3 cut(s) 161, 284, 702
HpySE526I ACGT 2 cut(s) 642, 828
Hsp92II CATG 5 cut(s) 20, 237, 419, 542, 807
HspAI GCGC 1 cut(s) 792
KpnI GGTACC 1 cut(s) 222
Kzo9I GATC 2 cut(s) 135, 510
LmnI GCTCC 1 cut(s) 272
Lsp1109I GCAGC 2 cut(s) 25, 395
LweI GCATC 3 cut(s) 199, 369, 420
MaeII ACGT 2 cut(s) 642, 828
MaeIII GTNAC 2 cut(s) 175, 861
MalI GATC 2 cut(s) 137, 512
MboI GATC 2 cut(s) 135, 510
MboII GAAGA 2 cut(s) 436, 507
MfeI CAATTG 2 cut(s) 472, 879
MflI RGATCY 1 cut(s) 135
MhlI GDGCHC 1 cut(s) 205
MlsI TGGCCA 2 cut(s) 144, 537
MluCI AATT 7 cut(s) 99, 239, 422, 472, 617, 778, 879
MluNI TGGCCA 2 cut(s) 144, 537
MlyI GAGTC 2 cut(s) 289, 813
MnlI CCTC 5 cut(s) 191, 263, 463, 568, 579
Mox20I TGGCCA 2 cut(s) 144, 537
MscI TGGCCA 2 cut(s) 144, 537
MseI TTAA 2 cut(s) 309, 639
Msp20I TGGCCA 2 cut(s) 144, 537
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 3 cut(s) 133, 180, 739
MspR9I CCNGG 2 cut(s) 492, 534
MunI CAATTG 2 cut(s) 472, 879
MvaI CCWGG 2 cut(s) 492, 534
MwoI GCNNNNNNNGC 3 cut(s) 171, 209, 230
NdeI CATATG 1 cut(s) 734
NdeII GATC 2 cut(s) 135, 510
NlaIII CATG 5 cut(s) 20, 237, 419, 542, 807
NlaIV GGNNCC 4 cut(s) 137, 202, 220, 274
NmeAIII GCCGAG 1 cut(s) 615
NmuCI GTSAC 1 cut(s) 861
NspI RCATGY 2 cut(s) 20, 237
NspV TTCGAA 1 cut(s) 97
PaeI GCATGC 1 cut(s) 237
PfeI GAWTC 1 cut(s) 847
PflMI CCANNNNNTGG 1 cut(s) 809
PinAI ACCGGT 1 cut(s) 179
PkrI GCNGC 2 cut(s) 15, 385
PleI GAGTC 2 cut(s) 288, 813
PpsI GAGTC 2 cut(s) 288, 813
PshAI GACNNNNGTC 1 cut(s) 325
Psp6I CCWGG 2 cut(s) 490, 532
PspGI CCWGG 2 cut(s) 490, 532
PspN4I GGNNCC 4 cut(s) 137, 202, 220, 274
PspPI GGNCC 2 cut(s) 488, 530
PstI CTGCAG 1 cut(s) 15
PsuI RGATCY 1 cut(s) 135
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 3 cut(s) 21, 220, 560
RsaNI GTAC 3 cut(s) 20, 219, 559
SaqAI TTAA 2 cut(s) 309, 639
SatI GCNGC 2 cut(s) 14, 384
Sau3AI GATC 2 cut(s) 135, 510
Sau96I GGNCC 2 cut(s) 488, 530
SchI GAGTC 2 cut(s) 289, 813
ScrFI CCNGG 2 cut(s) 492, 534
SduI GDGCHC 1 cut(s) 205
SfaNI GCATC 3 cut(s) 199, 369, 420
SfcI CTRYAG 3 cut(s) 11, 51, 841
SfuI TTCGAA 1 cut(s) 97
SinI GGWCC 1 cut(s) 530
SphI GCATGC 1 cut(s) 237
Sse9I AATT 7 cut(s) 99, 239, 422, 472, 617, 778, 879
SsiI CCGC 1 cut(s) 222
StyD4I CCNGG 2 cut(s) 490, 532
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 1 cut(s) 901
TaiI ACGT 2 cut(s) 645, 831
TaqI TCGA 4 cut(s) 97, 482, 513, 822
TasI AATT 7 cut(s) 99, 239, 422, 472, 617, 778, 879
TfiI GAWTC 1 cut(s) 847
Tru1I TTAA 2 cut(s) 309, 639
Tru9I TTAA 2 cut(s) 309, 639
TscAI CASTG 3 cut(s) 409, 730, 904
TseFI GTSAC 1 cut(s) 861
TseI GCWGC 2 cut(s) 13, 383
Tsp45I GTSAC 1 cut(s) 861
TspGWI ACGGA 2 cut(s) 282, 612
TspRI CASTG 3 cut(s) 409, 730, 904
Van91I CCANNNNNTGG 1 cut(s) 809
VpaK11BI GGWCC 1 cut(s) 530
XapI RAATTY 1 cut(s) 778
XceI RCATGY 2 cut(s) 20, 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.