Prupe.7G106800_v2.0.a1

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0024568
Physical Location & Seq
Reverse (-)
13541888 .. 13543000
1113 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G106800.3_v2.0.a1.CDS.2

Sequence Viewer

Length: 558 bp
ATGGAAGAAACACGGGAGGCTGCTTTAGCTTGTTTTGATAATTTGTCAGAGGAGCAAAAGGATAAGGCATGGGAAGATTTTGAAGCAATGGATTTAGATGGTGATGGGACAATAACCCTCATCGATTACCAGCAGTTTTATTATGGCAGCTCTTACAGGATCCCCTTCCAGCAGCTCGACACTAACAGAGATGGCAAGTTGAATTTTCAGGAATTTAAAACTCTTTTCTATTTGTTGCTCAATTCCAGGGGCACAACACCATTAATACCAACACAAGATCAGCCAGAATCTTCCGAGTCCAATCAACCAAACTCTTCTCAGTCCAATACAAGGACATTCGGCTTATCAGAGGCAAAAATGGTCTTCACCTACCTCAAAGACAGCCGATTCGTCCTCCTAACATGTCTTGTGCTGGGATTGGGTGTCTGGTATCAAGTTAGCCGATCTAGTGAGGTTGTAATGAAAGCAATAGAGGATTTCAATAGCTGTTGGGCCTTTGCAAGTGCTGTAGCTTCGGTAGCTACTGGTCTCTTCGATTATGTGATCAATATATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

186

Amino Acids

21.01

Weight (kDa)

4.33

Isoelectric Point (pI)

53.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024568)

Species Orthologous Gene IDs
prunus_persica Prupe.7G106800_v2.0.a1 Prupe.7G106800_v2.0.a1 Prupe.7G106800_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 154, 167
AcsI RAATTY 2 cut(s) 202, 212
AfiI CCNNNNNNNGG 1 cut(s) 330
AflIII ACRYGT 1 cut(s) 401
AgsI TTSAA 3 cut(s) 83, 202, 481
AjnI CCWGG 1 cut(s) 245
AluBI AGCT 6 cut(s) 29, 150, 175, 486, 512, 521
AluI AGCT 6 cut(s) 29, 150, 175, 486, 512, 521
Alw26I GTCTC 1 cut(s) 533
AlwI GGATC 2 cut(s) 154, 167
AoxI GGCC 1 cut(s) 492
ApeKI GCWGC 3 cut(s) 20, 147, 172
ApoI RAATTY 2 cut(s) 202, 212
ArsI GACNNNNNNTTYG 2 cut(s) 371, 403
AseI ATTAAT 1 cut(s) 263
AspS9I GGNCC 1 cut(s) 492
AsuHPI GGTGA 2 cut(s) 113, 358
BaeGI GKGCMC 1 cut(s) 254
BamHI GGATCC 1 cut(s) 159
BbsI GAAGAC 1 cut(s) 355
BbvI GCAGC 3 cut(s) 7, 159, 184
BccI CCATC 3 cut(s) 92, 98, 185
BciT130I CCWGG 1 cut(s) 247
BclI TGATCA 1 cut(s) 543
BcoDI GTCTC 1 cut(s) 533
BfaI CTAG 1 cut(s) 447
BfmI CTRYAG 1 cut(s) 507
BisI GCNGC 3 cut(s) 21, 148, 173
BlsI GCNGC 3 cut(s) 22, 149, 174
Bme1390I CCNGG 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 492
BmiI GGNNCC 1 cut(s) 161
BmrFI CCNGG 1 cut(s) 247
BpiI GAAGAC 1 cut(s) 355
Bsa29I ATCGAT 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 533
BsaJI CCNNGG 1 cut(s) 246
Bsc4I CCNNNNNNNGG 1 cut(s) 330
Bse1I ACTGG 1 cut(s) 529
Bse3DI GCAATG 1 cut(s) 93
BseBI CCWGG 1 cut(s) 247
BseCI ATCGAT 1 cut(s) 123
BseDI CCNNGG 1 cut(s) 246
BseLI CCNNNNNNNGG 1 cut(s) 330
BseMI GCAATG 1 cut(s) 93
BseMII CTCAG 1 cut(s) 332
BseNI ACTGG 1 cut(s) 529
BseRI GAGGAG 1 cut(s) 65
BseSI GKGCMC 1 cut(s) 254
BseXI GCAGC 3 cut(s) 7, 159, 184
BseYI CCCAGC 1 cut(s) 412
BshFI GGCC 1 cut(s) 494
BshVI ATCGAT 1 cut(s) 123
BslFI GGGAC 1 cut(s) 121
BslI CCNNNNNNNGG 1 cut(s) 330
BsmAI GTCTC 1 cut(s) 533
BsmFI GGGAC 1 cut(s) 121
BsnI GGCC 1 cut(s) 494
Bso31I GGTCTC 1 cut(s) 533
Bsp1286I GDGCHC 1 cut(s) 254
Bsp143I GATC 4 cut(s) 159, 277, 443, 543
BspANI GGCC 1 cut(s) 494
BspCNI CTCAG 1 cut(s) 331
BspDI ATCGAT 1 cut(s) 123
BspLI GGNNCC 1 cut(s) 161
BspPI GGATC 2 cut(s) 154, 167
BspTNI GGTCTC 1 cut(s) 533
BsrDI GCAATG 1 cut(s) 93
BsrI ACTGG 1 cut(s) 529
BssECI CCNNGG 1 cut(s) 246
BssMI GATC 4 cut(s) 159, 277, 443, 543
Bst2UI CCWGG 1 cut(s) 247
Bst6I CTCTTC 2 cut(s) 319, 536
BstDEI CTNAG 1 cut(s) 318
BstKTI GATC 4 cut(s) 162, 280, 446, 546
BstMAI GTCTC 1 cut(s) 533
BstMBI GATC 4 cut(s) 159, 277, 443, 543
BstMWI GCNNNNNNNGC 2 cut(s) 26, 518
BstNI CCWGG 1 cut(s) 247
BstNSI RCATGY 1 cut(s) 405
BstSCI CCNGG 1 cut(s) 245
BstSFI CTRYAG 1 cut(s) 507
BstSLI GKGCMC 1 cut(s) 254
BstV1I GCAGC 3 cut(s) 7, 159, 184
BstV2I GAAGAC 1 cut(s) 355
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
Bsu15I ATCGAT 1 cut(s) 123
BsuRI GGCC 1 cut(s) 494
BsuTUI ATCGAT 1 cut(s) 123
Cfr13I GGNCC 1 cut(s) 492
ClaI ATCGAT 1 cut(s) 123
CviAII CATG 2 cut(s) 69, 402
DdeI CTNAG 1 cut(s) 318
DpnI GATC 4 cut(s) 161, 279, 445, 545
DpnII GATC 4 cut(s) 159, 277, 443, 543
DraI TTTAAA 1 cut(s) 217
Eam1104I CTCTTC 2 cut(s) 319, 536
EarI CTCTTC 2 cut(s) 319, 536
Eco31I GGTCTC 1 cut(s) 533
EcoRII CCWGG 1 cut(s) 245
FaeI CATG 2 cut(s) 72, 405
FaiI YATR 6 cut(s) 70, 144, 403, 540, 551, 556
FaqI GGGAC 1 cut(s) 121
FatI CATG 2 cut(s) 68, 401
FbaI TGATCA 1 cut(s) 543
Fnu4HI GCNGC 3 cut(s) 21, 148, 173
Fsp4HI GCNGC 3 cut(s) 21, 148, 173
FspBI CTAG 1 cut(s) 447
GluI GCNGC 3 cut(s) 21, 148, 173
GsaI CCCAGC 1 cut(s) 416
HaeIII GGCC 1 cut(s) 494
Hin1II CATG 2 cut(s) 72, 405
HinfI GANTC 3 cut(s) 287, 296, 387
HphI GGTGA 2 cut(s) 113, 358
Hpy188I TCNGA 3 cut(s) 49, 295, 349
Hpy188III TCNNGA 1 cut(s) 209
HpyAV CCTTC 1 cut(s) 175
HpyCH4V TGCA 1 cut(s) 500
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 518
HpyF3I CTNAG 1 cut(s) 318
Hsp92II CATG 2 cut(s) 72, 405
Ksp22I TGATCA 1 cut(s) 543
Kzo9I GATC 4 cut(s) 159, 277, 443, 543
LmnI GCTCC 1 cut(s) 52
Lsp1109I GCAGC 3 cut(s) 7, 159, 184
MaeI CTAG 1 cut(s) 447
MalI GATC 4 cut(s) 161, 279, 445, 545
MboI GATC 4 cut(s) 159, 277, 443, 543
MboII GAAGA 6 cut(s) 17, 86, 282, 306, 355, 523
MflI RGATCY 1 cut(s) 159
MhlI GDGCHC 1 cut(s) 254
MluCI AATT 4 cut(s) 40, 202, 212, 241
MlyI GAGTC 1 cut(s) 305
MnlI CCTC 8 cut(s) 10, 43, 128, 343, 383, 404, 445, 466
MseI TTAA 2 cut(s) 216, 263
MspR9I CCNGG 1 cut(s) 247
MvaI CCWGG 1 cut(s) 247
MwoI GCNNNNNNNGC 2 cut(s) 26, 518
NdeII GATC 4 cut(s) 159, 277, 443, 543
NlaIII CATG 2 cut(s) 72, 405
NlaIV GGNNCC 1 cut(s) 161
NspI RCATGY 1 cut(s) 405
PciI ACATGT 1 cut(s) 401
PfeI GAWTC 2 cut(s) 287, 387
PkrI GCNGC 3 cut(s) 22, 149, 174
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
PscI ACATGT 1 cut(s) 401
PshBI ATTAAT 1 cut(s) 263
Psp6I CCWGG 1 cut(s) 245
PspFI CCCAGC 1 cut(s) 412
PspGI CCWGG 1 cut(s) 245
PspN4I GGNNCC 1 cut(s) 161
PspPI GGNCC 1 cut(s) 492
PsuI RGATCY 1 cut(s) 159
SaqAI TTAA 2 cut(s) 216, 263
SatI GCNGC 3 cut(s) 21, 148, 173
Sau3AI GATC 4 cut(s) 159, 277, 443, 543
Sau96I GGNCC 1 cut(s) 492
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 1 cut(s) 247
SduI GDGCHC 1 cut(s) 254
SetI ASST 9 cut(s) 31, 152, 177, 371, 375, 456, 488, 514, 523
SfcI CTRYAG 1 cut(s) 507
Sse9I AATT 4 cut(s) 40, 202, 212, 241
SspMI CTAG 1 cut(s) 447
StyD4I CCNGG 1 cut(s) 245
TaqI TCGA 3 cut(s) 123, 177, 534
TasI AATT 4 cut(s) 40, 202, 212, 241
TfiI GAWTC 2 cut(s) 287, 387
Tru1I TTAA 2 cut(s) 216, 263
Tru9I TTAA 2 cut(s) 216, 263
TseI GCWGC 3 cut(s) 20, 147, 172
TspDTI ATGAA 1 cut(s) 476
VspI ATTAAT 1 cut(s) 263
XapI RAATTY 2 cut(s) 202, 212
XceI RCATGY 1 cut(s) 405
XspI CTAG 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.