Prupe.7G108400_v2.0.a1
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
N/A
Physical Location & Seq
Forward (+)
13681131 .. 13683195
2065 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G108400.1

Sequence Viewer

Length: 1272 bp
ATGATTTTGGTTACCCACAATGGCCAAGAGAAACAATGTTATGCTGAAGATATCACAGCCATGGTTCTTGATAAGATGCGGAAGATTGCCGAGAACTACCTGGGCTCAACTGTGAAAAGTGCAGTTATCACTGTCCCTGCTTACTTCAGTGACTCACAGCGTCGGGCAACAAAAGCTGCTGGTCTTTCTGCAGGCCTAAATGTGATGCGTATTATGAACGAACCGAGCGCTGCGGCCATTTCTTTTGGCCTTTACAAGAAGGCCGGGTGGAGTAGCCCGCGAAACGTGATGATATTTGATTTGGGTGGTGGCACTTTAGATGTGTCCTTGCTTACCATGAGCACTTCTGGTGACTTTCAAGTCAAGGCAACGGCTGGAGACACTCACCTTGGCGGCCAAGACTTCGATAACAGATTGGTGAACTACTGTGCTGAGAAATTCAAGAGGGAGCATAAATTGGACGTTAGTGGAAACAAGAGAGCTCTTAGGAGGTTAAAAAATGAATGTGAGAAGGCAAAGAAGAGACTCTCATTTGAGTCAGACATTGACGTTGAAATTGACTGTTTGTGTGAGAATACTGATTTCACCATAACTTTTACTCGTGCCATATTTGAACAAGTAAACATGGATTTATTCATCAAGTGTATGGATCCTGTGAAAAAGTGTTTGGCGTATGCAAAAATGAACATAAACGGTGTCGATGATGTTCTTCTTGCTGGCGGCTCTTCTAGAATTCCAATGGTGCAGCAGCTATTACAGAAGTTTTTCAAGGGGAAGGAGCTGTGCAAGGGCGTCAATCCAGATGAGGCAGTGGCGTATGGTGCCGCTATTCAAGCTGCAGCCTTGAGTGGGAATGGAAAGGGGAAGTTTATTCAAGACTTCACTCTCAAGGATGTCACTCCTCTGCCGCTTGTTATGGAGGGTACAGATGTTAAAGGTATGAAAAAATTAATGAATTTGATTCCAAGAAACACTATAATTCCCGTGAGGAAGTGCAGAGAATTTTGTACCGTTAAGGACAACCAGGTTTTGATTGACTTCCGCATATATGAAGGTGAGAGTAGCATACCAGCAAATCTCAACTTTTTGGCTGAATGCAGCTTCCATGGCATTCCTCCAGCTCCCAAACATGTTCATATATTCCATGTTTTCTTCGAAATTGATGCCGACGGTATCTTGAGTGTCTCTGCTGTCAATAAGTCCACTGGCCAGAAGTATGAGATGATAATCAACCGAGACAGGCTGAAGAACCTTGAGGGAGATGAGACTTAA

Protein Analysis

424

Amino Acids

46.85

Weight (kDa)

8.36

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 359
AccB1I GGYRCC 1 cut(s) 821
AccII CGCG 1 cut(s) 280
AciI CCGC 8 cut(s) 79, 233, 278, 393, 720, 825, 908, 1042
AclWI GGATC 2 cut(s) 644, 657
AcoI YGGCCR 4 cut(s) 22, 234, 394, 1207
AcsI RAATTY 4 cut(s) 437, 732, 955, 1001
AcuI CTGAAG 3 cut(s) 66, 130, 1265
AcyI GRCGYC 1 cut(s) 792
AfaI GTAC 2 cut(s) 925, 1009
AfeI AGCGCT 1 cut(s) 229
AfiI CCNNNNNNNGG 1 cut(s) 849
AflIII ACRYGT 1 cut(s) 1129
AgsI TTSAA 7 cut(s) 359, 442, 554, 614, 769, 833, 875
AjnI CCWGG 2 cut(s) 99, 1023
AjuI GAANNNNNNNTTGG 2 cut(s) 650, 682
AluBI AGCT 7 cut(s) 176, 482, 751, 781, 836, 1101, 1121
AluI AGCT 7 cut(s) 176, 482, 751, 781, 836, 1101, 1121
Alw21I GWGCWC 2 cut(s) 344, 484
Alw26I GTCTC 5 cut(s) 372, 517, 1189, 1230, 1259
AlwI GGATC 2 cut(s) 644, 657
Aor51HI AGCGCT 1 cut(s) 229
AoxI GGCC 7 cut(s) 22, 193, 234, 247, 261, 394, 1207
ApeKI GCWGC 7 cut(s) 176, 230, 745, 748, 836, 839, 1098
ApoI RAATTY 4 cut(s) 437, 732, 955, 1001
AseI ATTAAT 1 cut(s) 950
Asp700I GAANNNNTTC 1 cut(s) 764
AspLEI GCGC 1 cut(s) 230
AsuC2I CCSGG 1 cut(s) 265
AsuHPI GGTGA 5 cut(s) 362, 377, 430, 577, 1067
AsuII TTCGAA 1 cut(s) 1155
BaeI ACNNNNGTAYC 2 cut(s) 915, 948
BalI TGGCCA 2 cut(s) 24, 1209
BamHI GGATCC 1 cut(s) 649
BanI GGYRCC 1 cut(s) 821
BanII GRGCYC 2 cut(s) 107, 484
BauI CACGAG 1 cut(s) 600
Bbv12I GWGCWC 2 cut(s) 344, 484
BbvI GCAGC 7 cut(s) 163, 217, 757, 760, 823, 851, 1110
BceAI ACGGC 1 cut(s) 387
BcgI CGANNNNNNTGC 2 cut(s) 1145, 1179
BciT130I CCWGG 2 cut(s) 101, 1025
BcnI CCSGG 1 cut(s) 265
BcoDI GTCTC 5 cut(s) 372, 517, 1189, 1230, 1259
BfaI CTAG 1 cut(s) 729
BfmI CTRYAG 2 cut(s) 189, 837
BfoI RGCGCY 1 cut(s) 231
Bme1390I CCNGG 3 cut(s) 101, 265, 1025
BmiI GGNNCC 2 cut(s) 651, 823
BmrFI CCNGG 3 cut(s) 101, 265, 1025
BmsI GCATC 3 cut(s) 66, 195, 1153
BpmI CTGGAG 2 cut(s) 396, 1101
Bpu14I TTCGAA 1 cut(s) 1155
BpuEI CTTGAG 3 cut(s) 865, 872, 1198
BpuMI CCSGG 1 cut(s) 265
BsaBI GATNNNNATC 1 cut(s) 1226
BsaHI GRCGYC 1 cut(s) 792
BsaJI CCNNGG 4 cut(s) 60, 100, 388, 1105
Bsc4I CCNNNNNNNGG 1 cut(s) 849
Bse1I ACTGG 1 cut(s) 1210
Bse8I GATNNNNATC 1 cut(s) 1226
BseBI CCWGG 2 cut(s) 101, 1025
BseDI CCNNGG 4 cut(s) 60, 100, 388, 1105
BseGI GGATG 1 cut(s) 898
BseJI GATNNNNATC 1 cut(s) 1226
BseLI CCNNNNNNNGG 1 cut(s) 849
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 1 cut(s) 1210
BseRI GAGGAG 1 cut(s) 891
BseXI GCAGC 7 cut(s) 163, 217, 757, 760, 823, 851, 1110
BsgI GTGCAG 3 cut(s) 141, 764, 1015
Bsh1236I CGCG 1 cut(s) 280
BshFI GGCC 7 cut(s) 24, 195, 236, 249, 263, 396, 1209
BshNI GGYRCC 1 cut(s) 821
BsiHKAI GWGCWC 2 cut(s) 344, 484
BsiSI CCGG 1 cut(s) 264
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 1 cut(s) 849
BsmAI GTCTC 5 cut(s) 372, 517, 1189, 1230, 1259
BsmFI GGGAC 1 cut(s) 119
BsmI GAATGC 2 cut(s) 1100, 1110
BsnI GGCC 7 cut(s) 24, 195, 236, 249, 263, 396, 1209
Bsp119I TTCGAA 1 cut(s) 1155
Bsp1286I GDGCHC 3 cut(s) 107, 344, 484
Bsp143I GATC 1 cut(s) 649
Bsp19I CCATGG 2 cut(s) 60, 1105
BspACI CCGC 8 cut(s) 79, 233, 278, 393, 720, 825, 908, 1042
BspANI GGCC 7 cut(s) 24, 195, 236, 249, 263, 396, 1209
BspCNI CTCAG 1 cut(s) 424
BspFNI CGCG 1 cut(s) 280
BspLI GGNNCC 2 cut(s) 651, 823
BspMAI CTGCAG 2 cut(s) 193, 841
BspPI GGATC 2 cut(s) 644, 657
BspQI GCTCTTC 1 cut(s) 730
BspT104I TTCGAA 1 cut(s) 1155
BspT107I GGYRCC 1 cut(s) 821
BsrI ACTGG 1 cut(s) 1210
BssECI CCNNGG 4 cut(s) 60, 100, 388, 1105
BssMI GATC 1 cut(s) 649
BssNI GRCGYC 1 cut(s) 792
BssSI CACGAG 1 cut(s) 600
BssT1I CCWWGG 3 cut(s) 60, 388, 1105
Bst2BI CACGAG 1 cut(s) 600
Bst2UI CCWGG 2 cut(s) 101, 1025
Bst4CI ACNGT 7 cut(s) 112, 133, 428, 563, 695, 1012, 1172
Bst6I CTCTTC 2 cut(s) 515, 730
BstACI GRCGYC 1 cut(s) 792
BstBI TTCGAA 1 cut(s) 1155
BstC8I GCNNGC 3 cut(s) 193, 278, 718
BstDEI CTNAG 2 cut(s) 432, 485
BstDSI CCRYGG 2 cut(s) 60, 1105
BstEII GGTNACC 1 cut(s) 10
BstF5I GGATG 1 cut(s) 898
BstFNI CGCG 1 cut(s) 280
BstH2I RGCGCY 1 cut(s) 231
BstHHI GCGC 1 cut(s) 230
BstKTI GATC 1 cut(s) 652
BstMAI GTCTC 5 cut(s) 372, 517, 1189, 1230, 1259
BstMBI GATC 1 cut(s) 649
BstMWI GCNNNNNNNGC 4 cut(s) 173, 821, 833, 1107
BstNI CCWGG 2 cut(s) 101, 1025
BstNSI RCATGY 1 cut(s) 1133
BstPI GGTNACC 1 cut(s) 10
BstSCI CCNGG 3 cut(s) 99, 263, 1023
BstSFI CTRYAG 2 cut(s) 189, 837
BstUI CGCG 1 cut(s) 280
BstV1I GCAGC 7 cut(s) 163, 217, 757, 760, 823, 851, 1110
BstX2I RGATCY 1 cut(s) 649
BstYI RGATCY 1 cut(s) 649
BsuRI GGCC 7 cut(s) 24, 195, 236, 249, 263, 396, 1209
BtgI CCRYGG 2 cut(s) 60, 1105
BtsCI GGATG 1 cut(s) 898
BtsI GCAGTG 1 cut(s) 816
BtsIMutI CAGTG 4 cut(s) 129, 154, 816, 1203
Cac8I GCNNGC 3 cut(s) 193, 278, 718
CfoI GCGC 1 cut(s) 230
CseI GACGC 2 cut(s) 149, 781
CsiI ACCWGGT 1 cut(s) 1023
Csp6I GTAC 2 cut(s) 924, 1008
CviAII CATG 6 cut(s) 61, 337, 625, 1106, 1130, 1145
CviQI GTAC 2 cut(s) 924, 1008
DdeI CTNAG 2 cut(s) 432, 485
DpnI GATC 1 cut(s) 651
DpnII GATC 1 cut(s) 649
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
EaeI YGGCCR 4 cut(s) 22, 234, 394, 1207
Eam1104I CTCTTC 2 cut(s) 515, 730
EarI CTCTTC 2 cut(s) 515, 730
Ecl136II GAGCTC 1 cut(s) 482
Eco130I CCWWGG 3 cut(s) 60, 388, 1105
Eco147I AGGCCT 1 cut(s) 195
Eco24I GRGCYC 2 cut(s) 107, 484
Eco32I GATATC 1 cut(s) 52
Eco47III AGCGCT 1 cut(s) 229
Eco53kI GAGCTC 1 cut(s) 482
Eco57I CTGAAG 3 cut(s) 66, 130, 1265
Eco91I GGTNACC 1 cut(s) 10
EcoICRI GAGCTC 1 cut(s) 482
EcoO65I GGTNACC 1 cut(s) 10
EcoRI GAATTC 1 cut(s) 732
EcoRII CCWGG 2 cut(s) 99, 1023
EcoRV GATATC 1 cut(s) 52
EcoT14I CCWWGG 3 cut(s) 60, 388, 1105
EcoT38I GRGCYC 2 cut(s) 107, 484
ErhI CCWWGG 3 cut(s) 60, 388, 1105
FaeI CATG 6 cut(s) 64, 340, 628, 1109, 1133, 1148
FaqI GGGAC 1 cut(s) 119
FatI CATG 6 cut(s) 60, 336, 624, 1105, 1129, 1144
FauI CCCGC 1 cut(s) 285
FokI GGATG 1 cut(s) 905
FriOI GRGCYC 2 cut(s) 107, 484
FspBI CTAG 1 cut(s) 729
GlaI GCGC 1 cut(s) 229
GsuI CTGGAG 2 cut(s) 396, 1101
HaeII RGCGCY 1 cut(s) 231
HaeIII GGCC 7 cut(s) 24, 195, 236, 249, 263, 396, 1209
HapII CCGG 1 cut(s) 264
HgaI GACGC 2 cut(s) 149, 781
HhaI GCGC 1 cut(s) 230
Hin1I GRCGYC 1 cut(s) 792
Hin1II CATG 6 cut(s) 64, 340, 628, 1109, 1133, 1148
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HinfI GANTC 4 cut(s) 152, 525, 536, 961
HpaII CCGG 1 cut(s) 264
HphI GGTGA 5 cut(s) 362, 377, 430, 577, 1067
Hpy166II GTNNAC 3 cut(s) 421, 622, 1203
Hpy188I TCNGA 1 cut(s) 541
Hpy188III TCNNGA 6 cut(s) 68, 442, 729, 800, 875, 1177
Hpy8I GTNNAC 3 cut(s) 421, 622, 1203
Hpy99I CGWCG 2 cut(s) 165, 1172
HpyAV CCTTC 4 cut(s) 253, 505, 769, 1046
HpyCH4III ACNGT 7 cut(s) 112, 133, 428, 563, 695, 1012, 1172
HpyCH4IV ACGT 3 cut(s) 285, 462, 549
HpyCH4V TGCA 8 cut(s) 122, 191, 677, 745, 786, 839, 996, 1098
HpyF10VI GCNNNNNNNGC 4 cut(s) 173, 821, 833, 1107
HpyF3I CTNAG 2 cut(s) 432, 485
HpySE526I ACGT 3 cut(s) 285, 462, 549
Hsp92I GRCGYC 1 cut(s) 792
Hsp92II CATG 6 cut(s) 64, 340, 628, 1109, 1133, 1148
HspAI GCGC 1 cut(s) 228
Kzo9I GATC 1 cut(s) 649
LguI GCTCTTC 1 cut(s) 730
LmnI GCTCC 3 cut(s) 448, 778, 1126
Lsp1109I GCAGC 7 cut(s) 163, 217, 757, 760, 823, 851, 1110
LweI GCATC 3 cut(s) 66, 195, 1153
MabI ACCWGGT 1 cut(s) 1023
MaeI CTAG 1 cut(s) 729
MaeII ACGT 3 cut(s) 285, 462, 549
MaeIII GTNAC 4 cut(s) 10, 149, 350, 895
MalI GATC 1 cut(s) 651
MboI GATC 1 cut(s) 649
MboII GAAGA 7 cut(s) 59, 94, 532, 701, 717, 1144, 1258
MflI RGATCY 1 cut(s) 649
MhlI GDGCHC 3 cut(s) 107, 344, 484
MlsI TGGCCA 2 cut(s) 24, 1209
MluCI AATT 9 cut(s) 437, 455, 555, 732, 947, 955, 978, 1001, 1158
MluNI TGGCCA 2 cut(s) 24, 1209
MlyI GAGTC 3 cut(s) 146, 519, 545
MnlI CCTC 8 cut(s) 438, 483, 799, 912, 913, 981, 1125, 1249
Mox20I TGGCCA 2 cut(s) 24, 1209
MroXI GAANNNNTTC 1 cut(s) 764
MscI TGGCCA 2 cut(s) 24, 1209
MseI TTAA 5 cut(s) 494, 933, 950, 1014, 1270
MslI CAYNNNNRTG 1 cut(s) 59
Msp20I TGGCCA 2 cut(s) 24, 1209
MspI CCGG 1 cut(s) 264
MspR9I CCNGG 3 cut(s) 101, 265, 1025
Mva1269I GAATGC 2 cut(s) 1100, 1110
MvaI CCWGG 2 cut(s) 101, 1025
MvnI CGCG 1 cut(s) 280
MwoI GCNNNNNNNGC 4 cut(s) 173, 821, 833, 1107
NciI CCSGG 1 cut(s) 265
NcoI CCATGG 2 cut(s) 60, 1105
NdeII GATC 1 cut(s) 649
NlaIII CATG 6 cut(s) 64, 340, 628, 1109, 1133, 1148
NlaIV GGNNCC 2 cut(s) 651, 823
NmeAIII GCCGAG 1 cut(s) 115
NmuCI GTSAC 3 cut(s) 149, 350, 895
NspI RCATGY 1 cut(s) 1133
NspV TTCGAA 1 cut(s) 1155
PceI AGGCCT 1 cut(s) 195
PciI ACATGT 1 cut(s) 1129
PciSI GCTCTTC 1 cut(s) 730
PctI GAATGC 2 cut(s) 1100, 1110
PdmI GAANNNNTTC 1 cut(s) 764
PfeI GAWTC 1 cut(s) 961
PleI GAGTC 3 cut(s) 146, 519, 544
PpsI GAGTC 3 cut(s) 146, 519, 544
PscI ACATGT 1 cut(s) 1129
PshBI ATTAAT 1 cut(s) 950
Psp124BI GAGCTC 1 cut(s) 484
Psp6I CCWGG 2 cut(s) 99, 1023
PspEI GGTNACC 1 cut(s) 10
PspGI CCWGG 2 cut(s) 99, 1023
PspN4I GGNNCC 2 cut(s) 651, 823
PstI CTGCAG 2 cut(s) 193, 841
PsuI RGATCY 1 cut(s) 649
RsaI GTAC 2 cut(s) 925, 1009
RsaNI GTAC 2 cut(s) 924, 1008
RseI CAYNNNNRTG 1 cut(s) 59
SacI GAGCTC 1 cut(s) 484
SapI GCTCTTC 1 cut(s) 730
SaqAI TTAA 5 cut(s) 494, 933, 950, 1014, 1270
Sau3AI GATC 1 cut(s) 649
SchI GAGTC 3 cut(s) 146, 519, 545
ScrFI CCNGG 3 cut(s) 101, 265, 1025
SduI GDGCHC 3 cut(s) 107, 344, 484
SexAI ACCWGGT 1 cut(s) 1023
SfaNI GCATC 3 cut(s) 66, 195, 1153
SfcI CTRYAG 2 cut(s) 189, 837
SfuI TTCGAA 1 cut(s) 1155
SmiMI CAYNNNNRTG 1 cut(s) 59
SmlI CTYRAG 4 cut(s) 844, 887, 1177, 1253
SmoI CTYRAG 4 cut(s) 844, 887, 1177, 1253
Sse9I AATT 9 cut(s) 437, 455, 555, 732, 947, 955, 978, 1001, 1158
SseBI AGGCCT 1 cut(s) 195
SsiI CCGC 8 cut(s) 79, 233, 278, 393, 720, 825, 908, 1042
SspMI CTAG 1 cut(s) 729
SstI GAGCTC 1 cut(s) 484
StuI AGGCCT 1 cut(s) 195
StyD4I CCNGG 3 cut(s) 99, 263, 1023
StyI CCWWGG 3 cut(s) 60, 388, 1105
TaaI ACNGT 7 cut(s) 112, 133, 428, 563, 695, 1012, 1172
TaiI ACGT 3 cut(s) 288, 465, 552
TaqI TCGA 3 cut(s) 405, 699, 1155
TasI AATT 9 cut(s) 437, 455, 555, 732, 947, 955, 978, 1001, 1158
TauI GCSGC 5 cut(s) 236, 396, 723, 827, 910
TfiI GAWTC 1 cut(s) 961
Tru1I TTAA 5 cut(s) 494, 933, 950, 1014, 1270
Tru9I TTAA 5 cut(s) 494, 933, 950, 1014, 1270
TscAI CASTG 4 cut(s) 136, 154, 816, 1210
TseFI GTSAC 3 cut(s) 149, 350, 895
TseI GCWGC 7 cut(s) 176, 230, 745, 748, 836, 839, 1098
Tsp45I GTSAC 3 cut(s) 149, 350, 895
TspDTI ATGAA 8 cut(s) 230, 516, 625, 698, 956, 968, 1065, 1124
TspRI CASTG 4 cut(s) 136, 154, 816, 1210
VspI ATTAAT 1 cut(s) 950
XapI RAATTY 4 cut(s) 437, 732, 955, 1001
XbaI TCTAGA 1 cut(s) 728
XceI RCATGY 1 cut(s) 1133
XmnI GAANNNNTTC 1 cut(s) 764
XspI CTAG 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.