Prupe.7G179200_v2.0.a1

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
N/A
Physical Location & Seq
Reverse (-)
17621871 .. 17624772
2902 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G179200.1_v2.0.a1.CDS.2

Sequence Viewer

Length: 1251 bp
ATGGCTATTGAACATCAGTTATCTGCTGAAGCAGGCTCCAAATTCAATCAGTTGTGTGCGCTTAGGTCAAATGATATTGCAGATGAAGCCTGTTCTCATGTTCGAGCATTTCCATTGTTGAACCAGGCTGCAACCTTGGCCCCCAATGTCCCAGCCACGCATGAGCTGCATTTTGCAGTTCCAATGACAGGGGCAGTTCTTTGGACACTAAATGCACGCCTTGATTCAGCTATGTTATCTGTCCTGCTGCTGAGTCATTCCGAGGCGAGAATCGTCTTTGTAGACTACCAGTTACTTGAAATTGCCCAAGGAACACTTGATCTTCTCACCCAAAAAACAGATACAAAACCACCTATTCTTGTTTTAATTGCTGTTTCCGAGGGGTCATCTGATACATCTCCCACCATATGCACTTCAAACACCTATGAATATGGGAGTCTTCTTGAAACAGGAGATGGTGGATTTGAGATAAGAAGGCCAAGGAGTGAATGGGATCCTATCAGTGTAAATTATACCTCCGGCACAACACCCAGGCCAAAAGGGTACCGGGGTGCTTATCTTAACGCGTTGTCTACGGTTCTGCTTCATGGAATAGGCTCAATGCCTGTTTATCTATGGACAGTGTCTATGTTTCACTGCAATGGGTGGTGCCTCACTTGGGGGGTGGCTGCTCAGGGTGGAACTAACATCTGCCTCAGGAAAGTCAATCCAAAACACATATTTGACAACATAGTTCAATACAATGTCACACACATGGGAGGGGCACCAACTGTCTTGAACATGATTGTTAATTCACCGGTCAGTGACTGGAGGCCACTTCCTCGCAAGGTGGAAATAATGACGGGTGGTTCACCACCCATGCCACAGATCGTTTTCAGGATGGAAGAATTGGGGTTTGGAGTAAATCAATTGTATGGTCTAACAGAATCTTATGGTCCAGGGACTTATTGCTCATTGAAACCAGAGTGGGATTCTCTGCCTTCCATTGAAGGATCAAAGCTTAAAGCTCGACAAGGGGTGCAGCATCTTGGTTTGGAAGAGGTTGACATAAAAGATCCTGTCACTATGGAAAGTGTAACACCTGATGGTAATACAGTGGGTGAGATTATGTTAAGGGGAAACACTGTTATGAGTGGATGGTTGGGGAGCGGGGATCTAGCTGTGAAACATCCAGATAATTACATTGATCGAAGTGAAGGACCGGTTGAAAGATATAATCATATACGGGGGAGAGAACATAAGTACAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

417

Amino Acids

45.58

Weight (kDa)

5.71

Isoelectric Point (pI)

36.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 543
AccB1I GGYRCC 3 cut(s) 543, 648, 763
AccBSI CCGCTC 1 cut(s) 1149
AccI GTMKAC 2 cut(s) 282, 572
AccII CGCG 1 cut(s) 566
AciI CCGC 1 cut(s) 1149
AclWI GGATC 5 cut(s) 488, 501, 1000, 1049, 1161
AcsI RAATTY 1 cut(s) 41
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 2 cut(s) 545, 1244
AfiI CCNNNNNNNGG 2 cut(s) 188, 658
AflIII ACRYGT 1 cut(s) 564
AgeI ACCGGT 2 cut(s) 796, 1201
AjnI CCWGG 3 cut(s) 123, 530, 937
AjuI GAANNNNNNNTTGG 2 cut(s) 879, 911
AluBI AGCT 5 cut(s) 166, 230, 1000, 1007, 1160
AluI AGCT 5 cut(s) 166, 230, 1000, 1007, 1160
AlwI GGATC 5 cut(s) 488, 501, 1000, 1049, 1161
AlwNI CAGNNNCTG 1 cut(s) 807
AoxI GGCC 4 cut(s) 138, 476, 533, 812
ApeKI GCWGC 5 cut(s) 128, 166, 247, 668, 1021
ApoI RAATTY 1 cut(s) 41
AsiGI ACCGGT 2 cut(s) 796, 1201
Asp718I GGTACC 1 cut(s) 543
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 3 cut(s) 139, 935, 1199
AsuC2I CCSGG 1 cut(s) 548
AsuHPI GGTGA 4 cut(s) 319, 786, 843, 1112
AvaII GGWCC 2 cut(s) 935, 1199
AxyI CCTNAGG 1 cut(s) 695
BaeGI GKGCMC 1 cut(s) 766
BaeI ACNNNNGTAYC 2 cut(s) 535, 568
BamHI GGATCC 1 cut(s) 493
BanI GGYRCC 3 cut(s) 543, 648, 763
BbsI GAAGAC 1 cut(s) 431
BbvI GCAGC 5 cut(s) 115, 153, 234, 655, 1033
BccI CCATC 4 cut(s) 449, 874, 1079, 1131
BciT130I CCWGG 3 cut(s) 125, 532, 939
BcnI CCSGG 1 cut(s) 548
BfaI CTAG 1 cut(s) 1157
BisI GCNGC 5 cut(s) 129, 167, 248, 669, 1022
BlsI GCNGC 5 cut(s) 130, 168, 249, 670, 1023
Bme1390I CCNGG 4 cut(s) 125, 532, 548, 939
Bme18I GGWCC 2 cut(s) 935, 1199
BmgT120I GGNCC 3 cut(s) 139, 935, 1199
BmiI GGNNCC 6 cut(s) 37, 141, 495, 545, 650, 765
BmrFI CCNGG 4 cut(s) 125, 532, 548, 939
BmsI GCATC 1 cut(s) 1033
BpiI GAAGAC 1 cut(s) 431
BpmI CTGGAG 1 cut(s) 829
Bpu10I CCTNAGC 2 cut(s) 62, 672
BpuMI CCSGG 1 cut(s) 548
BsaJI CCNNGG 8 cut(s) 135, 261, 307, 378, 479, 530, 547, 938
BsaWI WCCGGW 2 cut(s) 796, 1201
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 658
Bse118I RCCGGY 2 cut(s) 796, 1201
Bse1I ACTGG 2 cut(s) 289, 812
Bse21I CCTNAGG 1 cut(s) 695
Bse3DI GCAATG 1 cut(s) 646
BseBI CCWGG 3 cut(s) 125, 532, 939
BseDI CCNNGG 8 cut(s) 135, 261, 307, 378, 479, 530, 547, 938
BseGI GGATG 3 cut(s) 885, 1142, 1168
BseLI CCNNNNNNNGG 2 cut(s) 188, 658
BseMI GCAATG 1 cut(s) 646
BseMII CTCAG 3 cut(s) 242, 686, 709
BseNI ACTGG 2 cut(s) 289, 812
BseSI GKGCMC 1 cut(s) 766
BseXI GCAGC 5 cut(s) 115, 153, 234, 655, 1033
BseYI CCCAGC 1 cut(s) 151
BsgI GTGCAG 1 cut(s) 1040
Bsh1236I CGCG 1 cut(s) 566
BshFI GGCC 4 cut(s) 140, 478, 535, 814
BshNI GGYRCC 3 cut(s) 543, 648, 763
BshTI ACCGGT 2 cut(s) 796, 1201
BsiSI CCGG 4 cut(s) 519, 547, 797, 1202
BslFI GGGAC 2 cut(s) 134, 955
BslI CCNNNNNNNGG 2 cut(s) 188, 658
BsmFI GGGAC 2 cut(s) 134, 955
BsnI GGCC 4 cut(s) 140, 478, 535, 814
Bsp1286I GDGCHC 1 cut(s) 766
Bsp143I GATC 7 cut(s) 319, 493, 867, 992, 1054, 1153, 1186
BspACI CCGC 1 cut(s) 1149
BspANI GGCC 4 cut(s) 140, 478, 535, 814
BspCNI CTCAG 3 cut(s) 243, 685, 708
BspFNI CGCG 1 cut(s) 566
BspLI GGNNCC 6 cut(s) 37, 141, 495, 545, 650, 765
BspPI GGATC 5 cut(s) 488, 501, 1000, 1049, 1161
BspT107I GGYRCC 3 cut(s) 543, 648, 763
BsrBI CCGCTC 1 cut(s) 1149
BsrDI GCAATG 1 cut(s) 646
BsrFI RCCGGY 2 cut(s) 796, 1201
BsrI ACTGG 2 cut(s) 289, 812
BssAI RCCGGY 2 cut(s) 796, 1201
BssECI CCNNGG 8 cut(s) 135, 261, 307, 378, 479, 530, 547, 938
BssMI GATC 7 cut(s) 319, 493, 867, 992, 1054, 1153, 1186
BssT1I CCWWGG 3 cut(s) 135, 307, 479
Bst2UI CCWGG 3 cut(s) 125, 532, 939
Bst4CI ACNGT 6 cut(s) 577, 622, 772, 1096, 1126, 1247
Bst6I CTCTTC 1 cut(s) 1032
BstAPI GCANNNNNTGC 1 cut(s) 166
BstC8I GCNNGC 2 cut(s) 34, 217
BstDEI CTNAG 4 cut(s) 62, 251, 672, 695
BstF5I GGATG 3 cut(s) 885, 1142, 1168
BstFNI CGCG 1 cut(s) 566
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 7 cut(s) 322, 496, 870, 995, 1057, 1156, 1189
BstMBI GATC 7 cut(s) 319, 493, 867, 992, 1054, 1153, 1186
BstMWI GCNNNNNNNGC 3 cut(s) 86, 137, 166
BstNI CCWGG 3 cut(s) 125, 532, 939
BstSCI CCNGG 4 cut(s) 123, 530, 546, 937
BstSLI GKGCMC 1 cut(s) 766
BstUI CGCG 1 cut(s) 566
BstV1I GCAGC 5 cut(s) 115, 153, 234, 655, 1033
BstV2I GAAGAC 1 cut(s) 431
BstX2I RGATCY 3 cut(s) 493, 1054, 1153
BstYI RGATCY 3 cut(s) 493, 1054, 1153
Bsu36I CCTNAGG 1 cut(s) 695
BsuRI GGCC 4 cut(s) 140, 478, 535, 814
BtsCI GGATG 3 cut(s) 885, 1142, 1168
BtsI GCAGTG 1 cut(s) 634
BtsIMutI CAGTG 6 cut(s) 508, 627, 634, 808, 1101, 1122
Cac8I GCNNGC 2 cut(s) 34, 217
CaiI CAGNNNCTG 1 cut(s) 807
CfoI GCGC 1 cut(s) 61
Cfr10I RCCGGY 2 cut(s) 796, 1201
Cfr13I GGNCC 3 cut(s) 139, 935, 1199
Csp6I GTAC 2 cut(s) 544, 1243
CspAI ACCGGT 2 cut(s) 796, 1201
CviAII CATG 6 cut(s) 98, 161, 587, 754, 781, 859
CviQI GTAC 2 cut(s) 544, 1243
DdeI CTNAG 4 cut(s) 62, 251, 672, 695
DpnI GATC 7 cut(s) 321, 495, 869, 994, 1056, 1155, 1188
DpnII GATC 7 cut(s) 319, 493, 867, 992, 1054, 1153, 1186
Eam1104I CTCTTC 1 cut(s) 1032
EarI CTCTTC 1 cut(s) 1032
Eco130I CCWWGG 3 cut(s) 135, 307, 479
Eco47I GGWCC 2 cut(s) 935, 1199
Eco57I CTGAAG 1 cut(s) 48
Eco81I CCTNAGG 1 cut(s) 695
EcoRII CCWGG 3 cut(s) 123, 530, 937
EcoT14I CCWWGG 3 cut(s) 135, 307, 479
ErhI CCWWGG 3 cut(s) 135, 307, 479
FaeI CATG 6 cut(s) 101, 164, 590, 757, 784, 862
FalI AAGNNNNNCTT 2 cut(s) 300, 332
FaqI GGGAC 2 cut(s) 134, 955
FatI CATG 6 cut(s) 97, 160, 586, 753, 780, 858
FauI CCCGC 1 cut(s) 1142
FauNDI CATATG 1 cut(s) 407
FblI GTMKAC 2 cut(s) 282, 572
Fnu4HI GCNGC 5 cut(s) 129, 167, 248, 669, 1022
FokI GGATG 3 cut(s) 892, 1149, 1155
Fsp4HI GCNGC 5 cut(s) 129, 167, 248, 669, 1022
FspBI CTAG 1 cut(s) 1157
GlaI GCGC 1 cut(s) 60
GluI GCNGC 5 cut(s) 129, 167, 248, 669, 1022
GsaI CCCAGC 1 cut(s) 155
GsuI CTGGAG 1 cut(s) 829
HaeIII GGCC 4 cut(s) 140, 478, 535, 814
HapII CCGG 4 cut(s) 519, 547, 797, 1202
HhaI GCGC 1 cut(s) 61
Hin1II CATG 6 cut(s) 101, 164, 590, 757, 784, 862
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HincII GTYRAC 1 cut(s) 1045
HindII GTYRAC 1 cut(s) 1045
HindIII AAGCTT 1 cut(s) 998
HinfI GANTC 6 cut(s) 224, 253, 270, 436, 926, 971
HpaII CCGG 4 cut(s) 519, 547, 797, 1202
HphI GGTGA 4 cut(s) 319, 786, 843, 1112
Hpy166II GTNNAC 4 cut(s) 283, 573, 851, 1045
Hpy188I TCNGA 3 cut(s) 262, 379, 391
Hpy188III TCNNGA 5 cut(s) 443, 697, 775, 877, 1172
Hpy8I GTNNAC 4 cut(s) 283, 573, 851, 1045
HpyAV CCTTC 4 cut(s) 468, 983, 990, 1190
HpyCH4III ACNGT 6 cut(s) 577, 622, 772, 1096, 1126, 1247
HpyCH4V TGCA 8 cut(s) 80, 131, 169, 176, 215, 411, 639, 1021
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 137, 166
HpyF3I CTNAG 4 cut(s) 62, 251, 672, 695
Hsp92II CATG 6 cut(s) 101, 164, 590, 757, 784, 862
HspAI GCGC 1 cut(s) 59
KpnI GGTACC 1 cut(s) 547
Kzo9I GATC 7 cut(s) 319, 493, 867, 992, 1054, 1153, 1186
LmnI GCTCC 2 cut(s) 41, 1146
Lsp1109I GCAGC 5 cut(s) 115, 153, 234, 655, 1033
LweI GCATC 1 cut(s) 1033
MaeI CTAG 1 cut(s) 1157
MaeIII GTNAC 5 cut(s) 291, 745, 803, 1060, 1075
MalI GATC 7 cut(s) 321, 495, 869, 994, 1056, 1155, 1188
MbiI CCGCTC 1 cut(s) 1149
MboI GATC 7 cut(s) 319, 493, 867, 992, 1054, 1153, 1186
MboII GAAGA 4 cut(s) 314, 431, 896, 1049
MfeI CAATTG 1 cut(s) 908
MflI RGATCY 3 cut(s) 493, 1054, 1153
MhlI GDGCHC 1 cut(s) 766
MluCI AATT 8 cut(s) 41, 300, 366, 508, 790, 887, 908, 1177
MluI ACGCGT 1 cut(s) 564
MlyI GAGTC 2 cut(s) 262, 445
MnlI CCTC 9 cut(s) 256, 373, 526, 662, 704, 752, 804, 831, 1033
MseI TTAA 5 cut(s) 365, 561, 789, 1002, 1112
MslI CAYNNNNRTG 3 cut(s) 639, 752, 1127
MspI CCGG 4 cut(s) 519, 547, 797, 1202
MspR9I CCNGG 4 cut(s) 125, 532, 548, 939
MunI CAATTG 1 cut(s) 908
MvaI CCWGG 3 cut(s) 125, 532, 939
MvnI CGCG 1 cut(s) 566
MwoI GCNNNNNNNGC 3 cut(s) 86, 137, 166
NciI CCSGG 1 cut(s) 548
NdeI CATATG 1 cut(s) 407
NdeII GATC 7 cut(s) 319, 493, 867, 992, 1054, 1153, 1186
NlaIII CATG 6 cut(s) 101, 164, 590, 757, 784, 862
NlaIV GGNNCC 6 cut(s) 37, 141, 495, 545, 650, 765
NmuCI GTSAC 3 cut(s) 745, 803, 1060
PfeI GAWTC 4 cut(s) 224, 270, 926, 971
PflFI GACNNNGTC 1 cut(s) 622
PinAI ACCGGT 2 cut(s) 796, 1201
PkrI GCNGC 5 cut(s) 130, 168, 249, 670, 1023
PleI GAGTC 2 cut(s) 261, 444
PpsI GAGTC 2 cut(s) 261, 444
Psp6I CCWGG 3 cut(s) 123, 530, 937
PspFI CCCAGC 1 cut(s) 151
PspGI CCWGG 3 cut(s) 123, 530, 937
PspN4I GGNNCC 6 cut(s) 37, 141, 495, 545, 650, 765
PspPI GGNCC 3 cut(s) 139, 935, 1199
PstNI CAGNNNCTG 1 cut(s) 807
PsuI RGATCY 3 cut(s) 493, 1054, 1153
PsyI GACNNNGTC 1 cut(s) 622
RsaI GTAC 2 cut(s) 545, 1244
RsaNI GTAC 2 cut(s) 544, 1243
RseI CAYNNNNRTG 3 cut(s) 639, 752, 1127
SaqAI TTAA 5 cut(s) 365, 561, 789, 1002, 1112
SatI GCNGC 5 cut(s) 129, 167, 248, 669, 1022
Sau3AI GATC 7 cut(s) 319, 493, 867, 992, 1054, 1153, 1186
Sau96I GGNCC 3 cut(s) 139, 935, 1199
SchI GAGTC 2 cut(s) 262, 445
ScrFI CCNGG 4 cut(s) 125, 532, 548, 939
SduI GDGCHC 1 cut(s) 766
SfaNI GCATC 1 cut(s) 1033
SinI GGWCC 2 cut(s) 935, 1199
SmiMI CAYNNNNRTG 3 cut(s) 639, 752, 1127
Sse9I AATT 8 cut(s) 41, 300, 366, 508, 790, 887, 908, 1177
SsiI CCGC 1 cut(s) 1149
SspMI CTAG 1 cut(s) 1157
StyD4I CCNGG 4 cut(s) 123, 530, 546, 937
StyI CCWWGG 3 cut(s) 135, 307, 479
TaaI ACNGT 6 cut(s) 577, 622, 772, 1096, 1126, 1247
TaqI TCGA 3 cut(s) 103, 1009, 1189
TasI AATT 8 cut(s) 41, 300, 366, 508, 790, 887, 908, 1177
TatI WGTACW 1 cut(s) 1242
TfiI GAWTC 4 cut(s) 224, 270, 926, 971
Tru1I TTAA 5 cut(s) 365, 561, 789, 1002, 1112
Tru9I TTAA 5 cut(s) 365, 561, 789, 1002, 1112
TscAI CASTG 6 cut(s) 508, 627, 641, 808, 1101, 1129
TseFI GTSAC 3 cut(s) 745, 803, 1060
TseI GCWGC 5 cut(s) 128, 166, 247, 668, 1021
Tsp45I GTSAC 3 cut(s) 745, 803, 1060
TspDTI ATGAA 3 cut(s) 99, 441, 575
TspRI CASTG 6 cut(s) 508, 627, 641, 808, 1101, 1129
Tth111I GACNNNGTC 1 cut(s) 622
VpaK11BI GGWCC 2 cut(s) 935, 1199
XapI RAATTY 1 cut(s) 41
XcmI CCANNNNNNNNNTGG 1 cut(s) 486
XmiI GTMKAC 2 cut(s) 282, 572
XspI CTAG 1 cut(s) 1157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.