Prupe.7G257200_v2.0.a1

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
N/A
Physical Location & Seq
Reverse (-)
21520654 .. 21521413
760 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G257200.1_v2.0.a1.CDS.2

Sequence Viewer

Length: 636 bp
ATGGATGATGATGGTGACATTGATATTTTTTTCCCCAATTGGAACAACAAGAATCCAACTGATGATGGCCTTTTTATTGTCTCTTGCCTTATCGCTGAGTGGAGGAGAAATATCAATCTAAGTTGGATGAAAGCAATAGCCAGGTCAAAGAAAAATCCAAAGGCACGTCACAAAGTTCCTATAGCTCCTCATACTTTGGTTTTAGAATCGGTATCTCGTGGAAAGAACTTGAATTGCTGTGTATGCTTAAATTCCATGAGCCCTTCTCAAACTCTTGGCCCTACGACAGCTTCAGACAGTTTTAGTCACCGTTGCAGTATCTGTGGTGCAACGGCTCACCTAAGCTGCTCTTCTAGTAGAGATCAAACTGATGAAGCTTCTTTCTGCAGCTATTATGAAGAGCCATGTAGTGGGTCTTTTCTTGGTGGATCCCCAATATGGTGCTGCTTGTGGTGTCAAAGACTGGTACGTGTGGATTGCCACAGTAGCATGTCCAATGAAACATGTGATATTTGTGATTTAGGTCCATTCAGAAGCCAGACTTCCTCCGCTGGTGGATTTTTGAGCTCCATTACTCGGGGCCAATACCAACTGGCAAGCTTGCTCATCAACACCCACAACAGAACAAACATTTGA

Protein Analysis

212

Amino Acids

23.27

Weight (kDa)

6.95

Isoelectric Point (pI)

56.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 410
AciI CCGC 1 cut(s) 549
AclWI GGATC 2 cut(s) 423, 436
AcsI RAATTY 1 cut(s) 250
AcuI CTGAAG 1 cut(s) 276
AfaI GTAC 1 cut(s) 468
AfiI CCNNNNNNNGG 3 cut(s) 410, 438, 576
AflIII ACRYGT 2 cut(s) 469, 503
AgsI TTSAA 1 cut(s) 232
AjiI CACGTC 1 cut(s) 167
AjnI CCWGG 1 cut(s) 140
AluBI AGCT 7 cut(s) 185, 290, 345, 377, 390, 567, 600
AluI AGCT 7 cut(s) 185, 290, 345, 377, 390, 567, 600
Alw21I GWGCWC 1 cut(s) 569
Alw26I GTCTC 1 cut(s) 85
AlwI GGATC 2 cut(s) 423, 436
AlwNI CAGNNNCTG 1 cut(s) 321
Ama87I CYCGRG 1 cut(s) 576
AoxI GGCC 3 cut(s) 67, 277, 580
ApeKI GCWGC 3 cut(s) 345, 387, 444
ApoI RAATTY 1 cut(s) 250
AspS9I GGNCC 3 cut(s) 278, 524, 580
AsuHPI GGTGA 3 cut(s) 26, 299, 329
AvaI CYCGRG 1 cut(s) 576
AvaII GGWCC 1 cut(s) 524
BamHI GGATCC 1 cut(s) 428
BanII GRGCYC 2 cut(s) 263, 569
BauI CACGAG 1 cut(s) 216
Bbv12I GWGCWC 1 cut(s) 569
BbvI GCAGC 3 cut(s) 332, 399, 431
BccI CCATC 2 cut(s) 5, 59
BceAI ACGGC 1 cut(s) 348
BciT130I CCWGG 1 cut(s) 142
BcoDI GTCTC 1 cut(s) 85
BfaI CTAG 1 cut(s) 354
BfmI CTRYAG 2 cut(s) 180, 385
BisI GCNGC 3 cut(s) 346, 388, 445
BlsI GCNGC 3 cut(s) 347, 389, 446
Bme1390I CCNGG 1 cut(s) 142
Bme18I GGWCC 1 cut(s) 524
BmeT110I CYCGRG 1 cut(s) 576
BmgBI CACGTC 1 cut(s) 167
BmgT120I GGNCC 3 cut(s) 278, 524, 580
BmiI GGNNCC 2 cut(s) 430, 581
BmrFI CCNGG 1 cut(s) 142
BplI GAGNNNNNCTC 2 cut(s) 250, 282
Bpu10I CCTNAGC 1 cut(s) 341
BsaAI YACGTR 1 cut(s) 470
Bsc4I CCNNNNNNNGG 3 cut(s) 410, 438, 576
Bse1I ACTGG 2 cut(s) 468, 597
BseBI CCWGG 1 cut(s) 142
BseGI GGATG 2 cut(s) 10, 132
BseLI CCNNNNNNNGG 3 cut(s) 410, 438, 576
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 2 cut(s) 468, 597
BseRI GAGGAG 2 cut(s) 118, 177
BseXI GCAGC 3 cut(s) 332, 399, 431
BshFI GGCC 3 cut(s) 69, 279, 582
BsiHKAI GWGCWC 1 cut(s) 569
BsiHKCI CYCGRG 1 cut(s) 576
BslI CCNNNNNNNGG 3 cut(s) 410, 438, 576
BsmAI GTCTC 1 cut(s) 85
BsnI GGCC 3 cut(s) 69, 279, 582
BsoBI CYCGRG 1 cut(s) 576
Bsp1286I GDGCHC 2 cut(s) 263, 569
Bsp143I GATC 2 cut(s) 361, 428
BspACI CCGC 1 cut(s) 549
BspANI GGCC 3 cut(s) 69, 279, 582
BspCNI CTCAG 1 cut(s) 88
BspLI GGNNCC 2 cut(s) 430, 581
BspMAI CTGCAG 1 cut(s) 389
BspPI GGATC 2 cut(s) 423, 436
BspQI GCTCTTC 2 cut(s) 355, 393
BsrI ACTGG 2 cut(s) 468, 597
BssMI GATC 2 cut(s) 361, 428
BssSI CACGAG 1 cut(s) 216
Bst2BI CACGAG 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 3 cut(s) 299, 311, 485
Bst6I CTCTTC 2 cut(s) 355, 393
BstBAI YACGTR 1 cut(s) 470
BstC8I GCNNGC 2 cut(s) 598, 602
BstDEI CTNAG 3 cut(s) 96, 119, 341
BstF5I GGATG 2 cut(s) 10, 132
BstKTI GATC 2 cut(s) 364, 431
BstMAI GTCTC 1 cut(s) 85
BstMBI GATC 2 cut(s) 361, 428
BstMWI GCNNNNNNNGC 2 cut(s) 243, 486
BstNI CCWGG 1 cut(s) 142
BstNSI RCATGY 2 cut(s) 493, 507
BstSCI CCNGG 1 cut(s) 140
BstSFI CTRYAG 2 cut(s) 180, 385
BstV1I GCAGC 3 cut(s) 332, 399, 431
BstX2I RGATCY 1 cut(s) 428
BstYI RGATCY 1 cut(s) 428
BsuRI GGCC 3 cut(s) 69, 279, 582
BtrI CACGTC 1 cut(s) 167
BtsCI GGATG 2 cut(s) 10, 132
Cac8I GCNNGC 2 cut(s) 598, 602
CaiI CAGNNNCTG 1 cut(s) 321
Cfr13I GGNCC 3 cut(s) 278, 524, 580
Csp6I GTAC 1 cut(s) 467
CviAII CATG 4 cut(s) 256, 405, 490, 504
CviQI GTAC 1 cut(s) 467
DdeI CTNAG 3 cut(s) 96, 119, 341
DpnI GATC 2 cut(s) 363, 430
DpnII GATC 2 cut(s) 361, 428
Eam1104I CTCTTC 2 cut(s) 355, 393
EarI CTCTTC 2 cut(s) 355, 393
Ecl136II GAGCTC 1 cut(s) 567
Eco24I GRGCYC 2 cut(s) 263, 569
Eco47I GGWCC 1 cut(s) 524
Eco53kI GAGCTC 1 cut(s) 567
Eco57I CTGAAG 1 cut(s) 276
Eco88I CYCGRG 1 cut(s) 576
EcoICRI GAGCTC 1 cut(s) 567
EcoRII CCWGG 1 cut(s) 140
EcoT38I GRGCYC 2 cut(s) 263, 569
FaeI CATG 4 cut(s) 259, 408, 493, 507
FaiI YATR 9 cut(s) 182, 192, 244, 257, 396, 406, 439, 491, 505
FalI AAGNNNNNCTT 4 cut(s) 334, 366, 526, 558
FatI CATG 4 cut(s) 255, 404, 489, 503
Fnu4HI GCNGC 3 cut(s) 346, 388, 445
FokI GGATG 2 cut(s) 17, 139
FriOI GRGCYC 2 cut(s) 263, 569
Fsp4HI GCNGC 3 cut(s) 346, 388, 445
FspBI CTAG 1 cut(s) 354
GluI GCNGC 3 cut(s) 346, 388, 445
HaeIII GGCC 3 cut(s) 69, 279, 582
Hin1II CATG 4 cut(s) 259, 408, 493, 507
HindIII AAGCTT 2 cut(s) 375, 598
HinfI GANTC 2 cut(s) 52, 206
HphI GGTGA 3 cut(s) 26, 299, 329
Hpy188I TCNGA 2 cut(s) 295, 533
HpyAV CCTTC 1 cut(s) 273
HpyCH4III ACNGT 3 cut(s) 299, 311, 485
HpyCH4IV ACGT 2 cut(s) 166, 469
HpyCH4V TGCA 3 cut(s) 315, 329, 387
HpyF10VI GCNNNNNNNGC 2 cut(s) 243, 486
HpyF3I CTNAG 3 cut(s) 96, 119, 341
HpySE526I ACGT 2 cut(s) 166, 469
Hsp92II CATG 4 cut(s) 259, 408, 493, 507
Kzo9I GATC 2 cut(s) 361, 428
LguI GCTCTTC 2 cut(s) 355, 393
LmnI GCTCC 2 cut(s) 190, 572
LpnPI CCDG 6 cut(s) 127, 154, 449, 537, 551, 578
Lsp1109I GCAGC 3 cut(s) 332, 399, 431
MaeI CTAG 1 cut(s) 354
MaeII ACGT 2 cut(s) 166, 469
MaeIII GTNAC 3 cut(s) 14, 167, 305
MalI GATC 2 cut(s) 363, 430
MboI GATC 2 cut(s) 361, 428
MboII GAAGA 2 cut(s) 342, 410
MfeI CAATTG 1 cut(s) 37
MflI RGATCY 1 cut(s) 428
MhlI GDGCHC 2 cut(s) 263, 569
MluCI AATT 3 cut(s) 37, 232, 250
MmeI TCCRAC 2 cut(s) 80, 104
MnlI CCTC 3 cut(s) 96, 198, 556
MseI TTAA 1 cut(s) 248
MspA1I CMGCKG 1 cut(s) 551
MspR9I CCNGG 1 cut(s) 142
MunI CAATTG 1 cut(s) 37
MvaI CCWGG 1 cut(s) 142
MwoI GCNNNNNNNGC 2 cut(s) 243, 486
NdeII GATC 2 cut(s) 361, 428
NlaIII CATG 4 cut(s) 259, 408, 493, 507
NlaIV GGNNCC 2 cut(s) 430, 581
NmuCI GTSAC 3 cut(s) 14, 167, 305
NspI RCATGY 2 cut(s) 493, 507
PciI ACATGT 1 cut(s) 503
PciSI GCTCTTC 2 cut(s) 355, 393
PfeI GAWTC 2 cut(s) 52, 206
PflMI CCANNNNNTGG 1 cut(s) 410
PkrI GCNGC 3 cut(s) 347, 389, 446
Ppu21I YACGTR 1 cut(s) 470
PscI ACATGT 1 cut(s) 503
Psp124BI GAGCTC 1 cut(s) 569
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 2 cut(s) 430, 581
PspPI GGNCC 3 cut(s) 278, 524, 580
PstI CTGCAG 1 cut(s) 389
PstNI CAGNNNCTG 1 cut(s) 321
PsuI RGATCY 1 cut(s) 428
RsaI GTAC 1 cut(s) 468
RsaNI GTAC 1 cut(s) 467
SacI GAGCTC 1 cut(s) 569
SapI GCTCTTC 2 cut(s) 355, 393
SaqAI TTAA 1 cut(s) 248
SatI GCNGC 3 cut(s) 346, 388, 445
Sau3AI GATC 2 cut(s) 361, 428
Sau96I GGNCC 3 cut(s) 278, 524, 580
ScrFI CCNGG 1 cut(s) 142
SduI GDGCHC 2 cut(s) 263, 569
SfcI CTRYAG 2 cut(s) 180, 385
SinI GGWCC 1 cut(s) 524
Sse9I AATT 3 cut(s) 37, 232, 250
SsiI CCGC 1 cut(s) 549
SspMI CTAG 1 cut(s) 354
SstI GAGCTC 1 cut(s) 569
StyD4I CCNGG 1 cut(s) 140
TaaI ACNGT 3 cut(s) 299, 311, 485
TaiI ACGT 2 cut(s) 169, 472
TasI AATT 3 cut(s) 37, 232, 250
TfiI GAWTC 2 cut(s) 52, 206
Tru1I TTAA 1 cut(s) 248
Tru9I TTAA 1 cut(s) 248
TseFI GTSAC 3 cut(s) 14, 167, 305
TseI GCWGC 3 cut(s) 345, 387, 444
Tsp45I GTSAC 3 cut(s) 14, 167, 305
TspDTI ATGAA 4 cut(s) 143, 387, 411, 513
Van91I CCANNNNNTGG 1 cut(s) 410
VpaK11BI GGWCC 1 cut(s) 524
XapI RAATTY 1 cut(s) 250
XceI RCATGY 2 cut(s) 493, 507
XspI CTAG 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.