pycom01g03370

4,5-DOPA dioxygenase extradiol-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
N/A
Physical Location & Seq
Forward (+)
2610541 .. 2612022
1482 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1344 bp
ATGTGGGACTCCAAGTCCCACATAAAACACAAGTTCCAACACTGTGAACACTCATGTCTGGTACCTCTTGAAGAGCATGTCCATATAGATTTACTGCGGCACAACCCCACCACACACATTGATTCGTTTACATTTCGCAATTTTTTTTTTTTACAAGTTTGGGGGGTTTTGTCAATTTCGAATTTAGTATCATTTTGTATTTGGCATTTTTCTTCTGCATTATATGATTTTGGTATGTTTTACAATTATTATGTAAGGATATATTATTACTTTCATATCAACTTTTGTTATAATTATCATAAATCTCAAAACATAGATATAATACTATTGAGGTTCAAATTTTGGTTATTTTTTCAAATTTCCACTTTTTCTTTTTCTTATATTGCGATTATCTCTAATTTTACAGAGACCCGAATACCTCATCCTAAATCAAATTCACAAATTCCCAAATCCCTAATATTTCCAAATCGAAGAAATTTTCTTGCTGTTGGGTTTTCGTTTTTGTGGTCATTGAATTCTCAAAGAAAAAGGAAAAAGATTCTTATGGCAGTGAAGGACACGTTCTACCTATCGCACGGATCCCCCACGCTGAGCATCGACGAGTCGATTCCGGCCAGAAAATTCCTCCAGTCTTGGAAGGACACGGTCTATCCTCACAAACCCACCTCCATTCTCATCATCTCGGGACACTGGGAGACCGCCGTCCCCACCGTCAACTCCATTTCCGGCCGCCACGACACCATCTACGACTTCTACGGCTTCCCCAAATCCATGTACCAGCTCAAGTACCCTGCCCCCGGCTCCCCCCACCTCGCCAACTGCGTCAAGCAGCTGCTTACGTCCTCCGGCTTCGGCCCTGTGGACGTAGACAGCAAGCGGGGGCTCGACCATGGCGCATGGGTCCCGCTCATGTTGATGTACCCGGAGGGAGACATCCCGGTTTGCCAGTTGTCGATTCAGACAGAGAGGGACGCCACTTACCACTACAACATGGGGAGGGCGCTGGCTCCACTCAAGGAGGAGGGCATCCTTATAGTGGGGTCCGGTAGCGCCACTCACAACTTAAGGGCGTTGAAGAAGACAAAGGGCAATGGTGTTGTCCCTTGGGCTCTCGAGTTTGACACATGGTTGAAGGACGCTCTTCTCGAAGGAAGGTACGAGGATGTGAACAAGTACGTGGAGAAGGCACCGCATGCGAAAATGGCGCATCCTTGGCCGGATCACTTTTACCCGCTGCATGTAGCGGTGGGGGCTGCTGGTCCGGATGCGAAGGCTAAGCAAATCCACGACAGCTGGGAGCTCGGTGCTCTTTCGTATGCCTCCTATCAGTTTACATCACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00965 map00965
Pfam Domains
Protein Families
LigB

Protein Analysis

448

Amino Acids

51.56

Weight (kDa)

8.95

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LigB PF02900 187 - 444 1.6e-50 Catalytic LigB subunit of aromatic ring-opening dioxygenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 291
Acc65I GGTACC 1 cut(s) 61
AccB1I GGYRCC 2 cut(s) 61, 1186
AccBSI CCGCTC 1 cut(s) 907
AccI GTMKAC 1 cut(s) 867
AccIII TCCGGA 1 cut(s) 1261
AciI CCGC 8 cut(s) 97, 699, 730, 877, 905, 1190, 1232, 1244
AclWI GGATC 3 cut(s) 573, 586, 1227
AcoI YGGCCR 3 cut(s) 612, 727, 1214
AcsI RAATTY 8 cut(s) 181, 338, 357, 433, 441, 475, 514, 620
AcyI GRCGYC 1 cut(s) 972
AfaI GTAC 6 cut(s) 63, 776, 788, 920, 1157, 1175
AfiI CCNNNNNNNGG 2 cut(s) 797, 1036
AflII CTTAAG 1 cut(s) 1063
AflIII ACRYGT 1 cut(s) 558
AgsI TTSAA 6 cut(s) 71, 337, 356, 514, 1075, 1132
AhdI GACNNNNNGTC 1 cut(s) 13
AluBI AGCT 4 cut(s) 781, 832, 1293, 1300
AluI AGCT 4 cut(s) 781, 832, 1293, 1300
Alw21I GWGCWC 2 cut(s) 1302, 1309
Alw26I GTCTC 3 cut(s) 401, 689, 924
AlwI GGATC 3 cut(s) 573, 586, 1227
Ama87I CYCGRG 2 cut(s) 682, 1112
Aor13HI TCCGGA 1 cut(s) 1261
AoxI GGCC 4 cut(s) 612, 727, 853, 1214
ApeKI GCWGC 4 cut(s) 829, 832, 1234, 1253
ApoI RAATTY 8 cut(s) 181, 338, 357, 433, 441, 475, 514, 620
Asp718I GGTACC 1 cut(s) 61
AspLEI GCGC 4 cut(s) 896, 1003, 1052, 1207
AspS9I GGNCC 4 cut(s) 854, 901, 1041, 1259
AsuC2I CCSGG 3 cut(s) 798, 923, 938
AsuHPI GGTGA 1 cut(s) 1329
AsuII TTCGAA 1 cut(s) 179
AvaI CYCGRG 2 cut(s) 682, 1112
AvaII GGWCC 3 cut(s) 901, 1041, 1259
BamHI GGATCC 1 cut(s) 578
BanI GGYRCC 2 cut(s) 61, 1186
BanII GRGCYC 3 cut(s) 885, 1111, 1302
BbsI GAAGAC 1 cut(s) 1085
Bbv12I GWGCWC 2 cut(s) 1302, 1309
BbvI GCAGC 4 cut(s) 819, 841, 1221, 1240
BccI CCATC 1 cut(s) 749
BceAI ACGGC 2 cut(s) 686, 772
BcnI CCSGG 3 cut(s) 798, 923, 938
BcoDI GTCTC 3 cut(s) 401, 689, 924
BfoI RGCGCY 2 cut(s) 1004, 1053
BfrI CTTAAG 1 cut(s) 1063
BisI GCNGC 6 cut(s) 98, 730, 830, 833, 1235, 1254
BlpI GCTNAGC 2 cut(s) 590, 1275
BlsI GCNGC 6 cut(s) 99, 731, 831, 834, 1236, 1255
Bme1390I CCNGG 3 cut(s) 798, 923, 938
Bme18I GGWCC 3 cut(s) 901, 1041, 1259
BmeRI GACNNNNNGTC 1 cut(s) 13
BmeT110I CYCGRG 2 cut(s) 682, 1112
BmgT120I GGNCC 4 cut(s) 854, 901, 1041, 1259
BmiI GGNNCC 8 cut(s) 63, 580, 802, 902, 903, 1008, 1042, 1188
BmrFI CCNGG 3 cut(s) 798, 923, 938
BmrI ACTGGG 1 cut(s) 700
BmsI GCATC 4 cut(s) 603, 1035, 1216, 1255
BmuI ACTGGG 1 cut(s) 700
BoxI GACNNNNGTC 1 cut(s) 701
BpiI GAAGAC 1 cut(s) 1085
BpmI CTGGAG 1 cut(s) 611
Bpu1102I GCTNAGC 2 cut(s) 590, 1275
Bpu14I TTCGAA 1 cut(s) 179
BpuEI CTTGAG 2 cut(s) 767, 998
BpuMI CCSGG 3 cut(s) 798, 923, 938
BsaAI YACGTR 1 cut(s) 1177
BsaHI GRCGYC 1 cut(s) 972
BsaI GGTCTC 2 cut(s) 401, 689
BsaJI CCNNGG 4 cut(s) 796, 889, 1103, 1211
BsaWI WCCGGW 2 cut(s) 1043, 1261
Bsc4I CCNNNNNNNGG 2 cut(s) 797, 1036
Bse1I ACTGG 3 cut(s) 628, 695, 946
Bse3DI GCAATG 1 cut(s) 1096
BseAI TCCGGA 1 cut(s) 1261
BseDI CCNNGG 4 cut(s) 796, 889, 1103, 1211
BseGI GGATG 6 cut(s) 421, 933, 1026, 1168, 1207, 1270
BseLI CCNNNNNNNGG 2 cut(s) 797, 1036
BseMI GCAATG 1 cut(s) 1096
BseMII CTCAG 1 cut(s) 581
BseNI ACTGG 3 cut(s) 628, 695, 946
BseRI GAGGAG 1 cut(s) 1034
BseX3I CGGCCG 1 cut(s) 727
BseXI GCAGC 4 cut(s) 819, 841, 1221, 1240
BseYI CCCAGC 1 cut(s) 1293
Bsh1285I CGRYCG 1 cut(s) 730
BshFI GGCC 4 cut(s) 614, 729, 855, 1216
BshNI GGYRCC 2 cut(s) 61, 1186
BsiEI CGRYCG 1 cut(s) 730
BsiHKAI GWGCWC 2 cut(s) 1302, 1309
BsiHKCI CYCGRG 2 cut(s) 682, 1112
BsiSI CCGG 9 cut(s) 611, 726, 798, 846, 923, 938, 1044, 1217, 1262
BslFI GGGAC 6 cut(s) 20, 689, 699, 887, 983, 1085
BslI CCNNNNNNNGG 2 cut(s) 797, 1036
BsmAI GTCTC 3 cut(s) 401, 689, 924
BsmFI GGGAC 6 cut(s) 20, 689, 699, 887, 983, 1085
BsnI GGCC 4 cut(s) 614, 729, 855, 1216
Bso31I GGTCTC 2 cut(s) 401, 689
BsoBI CYCGRG 2 cut(s) 682, 1112
Bsp119I TTCGAA 1 cut(s) 179
Bsp1286I GDGCHC 4 cut(s) 885, 1111, 1302, 1309
Bsp13I TCCGGA 1 cut(s) 1261
Bsp143I GATC 2 cut(s) 578, 1219
Bsp1720I GCTNAGC 2 cut(s) 590, 1275
Bsp19I CCATGG 1 cut(s) 889
BspACI CCGC 8 cut(s) 97, 699, 730, 877, 905, 1190, 1232, 1244
BspANI GGCC 4 cut(s) 614, 729, 855, 1216
BspCNI CTCAG 1 cut(s) 582
BspEI TCCGGA 1 cut(s) 1261
BspLI GGNNCC 8 cut(s) 63, 580, 802, 902, 903, 1008, 1042, 1188
BspPI GGATC 3 cut(s) 573, 586, 1227
BspQI GCTCTTC 2 cut(s) 66, 1146
BspT104I TTCGAA 1 cut(s) 179
BspT107I GGYRCC 2 cut(s) 61, 1186
BspTI CTTAAG 1 cut(s) 1063
BspTNI GGTCTC 2 cut(s) 401, 689
BsrBI CCGCTC 1 cut(s) 907
BsrDI GCAATG 1 cut(s) 1096
BsrI ACTGG 3 cut(s) 628, 695, 946
BssECI CCNNGG 4 cut(s) 796, 889, 1103, 1211
BssMI GATC 2 cut(s) 578, 1219
BssNI GRCGYC 1 cut(s) 972
BssT1I CCWWGG 3 cut(s) 889, 1103, 1211
Bst4CI ACNGT 3 cut(s) 44, 646, 712
Bst6I CTCTTC 2 cut(s) 66, 1146
BstACI GRCGYC 1 cut(s) 972
BstAFI CTTAAG 1 cut(s) 1063
BstAPI GCANNNNNTGC 1 cut(s) 1193
BstBAI YACGTR 1 cut(s) 1177
BstBI TTCGAA 1 cut(s) 179
BstC8I GCNNGC 3 cut(s) 875, 1005, 1194
BstDEI CTNAG 2 cut(s) 590, 1275
BstDSI CCRYGG 1 cut(s) 889
BstF5I GGATG 6 cut(s) 421, 933, 1026, 1168, 1207, 1270
BstH2I RGCGCY 2 cut(s) 1004, 1053
BstHHI GCGC 4 cut(s) 896, 1003, 1052, 1207
BstKTI GATC 2 cut(s) 581, 1222
BstMAI GTCTC 3 cut(s) 401, 689, 924
BstMBI GATC 2 cut(s) 578, 1219
BstMCI CGRYCG 1 cut(s) 730
BstMWI GCNNNNNNNGC 4 cut(s) 1193, 1202, 1213, 1250
BstNSI RCATGY 3 cut(s) 80, 1196, 1241
BstPAI GACNNNNGTC 1 cut(s) 701
BstSCI CCNGG 3 cut(s) 796, 921, 936
BstV1I GCAGC 4 cut(s) 819, 841, 1221, 1240
BstV2I GAAGAC 1 cut(s) 1085
BstX2I RGATCY 1 cut(s) 578
BstYI RGATCY 1 cut(s) 578
BstZI CGGCCG 1 cut(s) 727
BsuRI GGCC 4 cut(s) 614, 729, 855, 1216
BtgI CCRYGG 1 cut(s) 889
BtsCI GGATG 6 cut(s) 421, 933, 1026, 1168, 1207, 1270
BtsI GCAGTG 1 cut(s) 555
BtsIMutI CAGTG 3 cut(s) 40, 555, 688
Cac8I GCNNGC 3 cut(s) 875, 1005, 1194
CfoI GCGC 4 cut(s) 896, 1003, 1052, 1207
Cfr13I GGNCC 4 cut(s) 854, 901, 1041, 1259
CseI GACGC 3 cut(s) 811, 980, 1145
Csp6I GTAC 6 cut(s) 62, 775, 787, 919, 1156, 1174
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviQI GTAC 6 cut(s) 62, 775, 787, 919, 1156, 1174
DdeI CTNAG 2 cut(s) 590, 1275
DpnI GATC 2 cut(s) 580, 1221
DpnII GATC 2 cut(s) 578, 1219
DriI GACNNNNNGTC 1 cut(s) 13
EaeI YGGCCR 3 cut(s) 612, 727, 1214
EagI CGGCCG 1 cut(s) 727
Eam1104I CTCTTC 2 cut(s) 66, 1146
Eam1105I GACNNNNNGTC 1 cut(s) 13
EarI CTCTTC 2 cut(s) 66, 1146
Ecl136II GAGCTC 1 cut(s) 1300
EclXI CGGCCG 1 cut(s) 727
Eco130I CCWWGG 3 cut(s) 889, 1103, 1211
Eco24I GRGCYC 3 cut(s) 885, 1111, 1302
Eco31I GGTCTC 2 cut(s) 401, 689
Eco47I GGWCC 3 cut(s) 901, 1041, 1259
Eco52I CGGCCG 1 cut(s) 727
Eco53kI GAGCTC 1 cut(s) 1300
Eco88I CYCGRG 2 cut(s) 682, 1112
EcoICRI GAGCTC 1 cut(s) 1300
EcoO109I RGGNCCY 1 cut(s) 901
EcoRI GAATTC 1 cut(s) 514
EcoT14I CCWWGG 3 cut(s) 889, 1103, 1211
EcoT38I GRGCYC 3 cut(s) 885, 1111, 1302
ErhI CCWWGG 3 cut(s) 889, 1103, 1211
FaqI GGGAC 6 cut(s) 20, 689, 699, 887, 983, 1085
FauI CCCGC 3 cut(s) 870, 912, 1239
FblI GTMKAC 1 cut(s) 867
Fnu4HI GCNGC 6 cut(s) 98, 730, 830, 833, 1235, 1254
FokI GGATG 6 cut(s) 408, 920, 1013, 1175, 1194, 1277
FriOI GRGCYC 3 cut(s) 885, 1111, 1302
Fsp4HI GCNGC 6 cut(s) 98, 730, 830, 833, 1235, 1254
GlaI GCGC 4 cut(s) 895, 1002, 1051, 1206
GluI GCNGC 6 cut(s) 98, 730, 830, 833, 1235, 1254
GsaI CCCAGC 1 cut(s) 1297
GsuI CTGGAG 1 cut(s) 611
HaeII RGCGCY 2 cut(s) 1004, 1053
HaeIII GGCC 4 cut(s) 614, 729, 855, 1216
HapII CCGG 9 cut(s) 611, 726, 798, 846, 923, 938, 1044, 1217, 1262
HgaI GACGC 3 cut(s) 811, 980, 1145
HhaI GCGC 4 cut(s) 896, 1003, 1052, 1207
Hin1I GRCGYC 1 cut(s) 972
Hin6I GCGC 4 cut(s) 894, 1001, 1050, 1205
HinP1I GCGC 4 cut(s) 894, 1001, 1050, 1205
HincII GTYRAC 1 cut(s) 715
HindII GTYRAC 1 cut(s) 715
HinfI GANTC 6 cut(s) 8, 122, 538, 602, 607, 955
HpaII CCGG 9 cut(s) 611, 726, 798, 846, 923, 938, 1044, 1217, 1262
HphI GGTGA 1 cut(s) 1329
Hpy166II GTNNAC 7 cut(s) 47, 129, 715, 862, 868, 1168, 1332
Hpy188I TCNGA 1 cut(s) 960
Hpy188III TCNNGA 5 cut(s) 68, 684, 1112, 1145, 1262
Hpy8I GTNNAC 7 cut(s) 47, 129, 715, 862, 868, 1168, 1332
Hpy99I CGWCG 1 cut(s) 602
HpyAV CCTTC 7 cut(s) 547, 631, 1126, 1142, 1146, 1177, 1264
HpyCH4III ACNGT 3 cut(s) 44, 646, 712
HpyCH4IV ACGT 4 cut(s) 560, 839, 864, 1176
HpyCH4V TGCA 2 cut(s) 218, 1237
HpyF10VI GCNNNNNNNGC 4 cut(s) 1193, 1202, 1213, 1250
HpyF3I CTNAG 2 cut(s) 590, 1275
HpySE526I ACGT 4 cut(s) 560, 839, 864, 1176
Hsp92I GRCGYC 1 cut(s) 972
HspAI GCGC 4 cut(s) 894, 1001, 1050, 1205
KflI GGGWCCC 1 cut(s) 901
Kpn2I TCCGGA 1 cut(s) 1261
KpnI GGTACC 1 cut(s) 65
Kzo9I GATC 2 cut(s) 578, 1219
LguI GCTCTTC 2 cut(s) 66, 1146
LmnI GCTCC 3 cut(s) 806, 1012, 1297
Lsp1109I GCAGC 4 cut(s) 819, 841, 1221, 1240
LweI GCATC 4 cut(s) 603, 1035, 1216, 1255
MaeII ACGT 4 cut(s) 560, 839, 864, 1176
MalI GATC 2 cut(s) 580, 1221
MbiI CCGCTC 1 cut(s) 907
MboI GATC 2 cut(s) 578, 1219
MboII GAAGA 6 cut(s) 83, 204, 483, 1087, 1090, 1133
MflI RGATCY 1 cut(s) 578
MhlI GDGCHC 4 cut(s) 885, 1111, 1302, 1309
MlyI GAGTC 2 cut(s) 2, 611
MmeI TCCRAC 1 cut(s) 61
MroI TCCGGA 1 cut(s) 1261
MseI TTAA 1 cut(s) 1064
MslI CAYNNNNRTG 1 cut(s) 914
MspA1I CMGCKG 3 cut(s) 832, 1234, 1293
MspCI CTTAAG 1 cut(s) 1063
MspI CCGG 9 cut(s) 611, 726, 798, 846, 923, 938, 1044, 1217, 1262
MspR9I CCNGG 3 cut(s) 798, 923, 938
MwoI GCNNNNNNNGC 4 cut(s) 1193, 1202, 1213, 1250
NciI CCSGG 3 cut(s) 798, 923, 938
NcoI CCATGG 1 cut(s) 889
NdeII GATC 2 cut(s) 578, 1219
NlaIV GGNNCC 8 cut(s) 63, 580, 802, 902, 903, 1008, 1042, 1188
NspI RCATGY 3 cut(s) 80, 1196, 1241
NspV TTCGAA 1 cut(s) 179
PaeI GCATGC 1 cut(s) 1196
PaeR7I CTCGAG 1 cut(s) 1112
PciSI GCTCTTC 2 cut(s) 66, 1146
PcsI WCGNNNNNNNCGW 2 cut(s) 819, 1143
PfeI GAWTC 4 cut(s) 122, 538, 607, 955
PflFI GACNNNGTC 1 cut(s) 644
PkrI GCNGC 6 cut(s) 99, 731, 831, 834, 1236, 1255
PleI GAGTC 2 cut(s) 2, 610
PpsI GAGTC 2 cut(s) 2, 610
Ppu21I YACGTR 1 cut(s) 1177
PpuMI RGGWCCY 1 cut(s) 901
PshAI GACNNNNGTC 1 cut(s) 701
PsiI TTATAA 1 cut(s) 291
Psp124BI GAGCTC 1 cut(s) 1302
Psp5II RGGWCCY 1 cut(s) 901
PspFI CCCAGC 1 cut(s) 1293
PspN4I GGNNCC 8 cut(s) 63, 580, 802, 902, 903, 1008, 1042, 1188
PspPI GGNCC 4 cut(s) 854, 901, 1041, 1259
PspPPI RGGWCCY 1 cut(s) 901
PsuI RGATCY 1 cut(s) 578
PsyI GACNNNGTC 1 cut(s) 644
PvuII CAGCTG 2 cut(s) 832, 1293
RsaI GTAC 6 cut(s) 63, 776, 788, 920, 1157, 1175
RsaNI GTAC 6 cut(s) 62, 775, 787, 919, 1156, 1174
RseI CAYNNNNRTG 1 cut(s) 914
SacI GAGCTC 1 cut(s) 1302
SapI GCTCTTC 2 cut(s) 66, 1146
SaqAI TTAA 1 cut(s) 1064
SatI GCNGC 6 cut(s) 98, 730, 830, 833, 1235, 1254
Sau3AI GATC 2 cut(s) 578, 1219
Sau96I GGNCC 4 cut(s) 854, 901, 1041, 1259
SchI GAGTC 2 cut(s) 2, 611
ScrFI CCNGG 3 cut(s) 798, 923, 938
SduI GDGCHC 4 cut(s) 885, 1111, 1302, 1309
SfaNI GCATC 4 cut(s) 603, 1035, 1216, 1255
Sfr274I CTCGAG 1 cut(s) 1112
SfuI TTCGAA 1 cut(s) 179
SinI GGWCC 3 cut(s) 901, 1041, 1259
SlaI CTCGAG 1 cut(s) 1112
SmiMI CAYNNNNRTG 1 cut(s) 914
SmlI CTYRAG 4 cut(s) 782, 1013, 1063, 1112
SmoI CTYRAG 4 cut(s) 782, 1013, 1063, 1112
SphI GCATGC 1 cut(s) 1196
SsiI CCGC 8 cut(s) 97, 699, 730, 877, 905, 1190, 1232, 1244
SspI AATATT 1 cut(s) 459
SstI GAGCTC 1 cut(s) 1302
StyD4I CCNGG 3 cut(s) 796, 921, 936
StyI CCWWGG 3 cut(s) 889, 1103, 1211
TaaI ACNGT 3 cut(s) 44, 646, 712
TaiI ACGT 4 cut(s) 563, 842, 867, 1179
TaqI TCGA 8 cut(s) 179, 469, 597, 605, 885, 953, 1113, 1146
TauI GCSGC 2 cut(s) 100, 732
TfiI GAWTC 4 cut(s) 122, 538, 607, 955
Tru1I TTAA 1 cut(s) 1064
Tru9I TTAA 1 cut(s) 1064
TscAI CASTG 3 cut(s) 47, 555, 695
TseI GCWGC 4 cut(s) 829, 832, 1234, 1253
TspDTI ATGAA 1 cut(s) 263
TspGWI ACGGA 1 cut(s) 591
TspRI CASTG 3 cut(s) 47, 555, 695
Tth111I GACNNNGTC 1 cut(s) 644
Vha464I CTTAAG 1 cut(s) 1063
VpaK11BI GGWCC 3 cut(s) 901, 1041, 1259
XapI RAATTY 8 cut(s) 181, 338, 357, 433, 441, 475, 514, 620
XceI RCATGY 3 cut(s) 80, 1196, 1241
XhoI CTCGAG 1 cut(s) 1112
XmiI GTMKAC 1 cut(s) 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.