pycom01g06270

LMBR1 domain-containing protein 2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
N/A
Physical Location & Seq
Forward (+)
6393594 .. 6400380
6787 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1242 bp
ATGTGGGTCTTCTACCTGATCTCTCTGCCCCTGACCTTGGGCATGGTTCTTTTCACGCTCAAGTACTTCGCCGGCCCGGAGGTCCCTCGCTATGTTATGATCACCGTCGGGTACACCTGGTTCTGTTCTCTCTCCATCATCGTCATTGTGCCCGCCGACATCTGGACGACGATAAACCATATTGAGAGTGGAGGAATATCTTTCTTTTGGAGCTGGTCATATTGGAGTACATTTTTACTAACTTGGACTGTGGTGCCCCTTATTCAGGGTTTTGAAGACGCTGGGGACTTCACCGTGACAGAGAGATTGAAGACTAGTGTACATGTTAACTTGGTTTTCTATCTAATTCTGGGGTTTATTGGTCTTATTGGATTAATTCTTCTCATCAGTATGCACAACTGTAACAGGGGTGGAGGTGTTCTTGGTTTTGCCATGGGCTGCTCGAATACTTTTGGACTTGTTACAGGTGCATTTCTTCTTGGATTTGGTTTGAGTGAAATCCCGAAGGGCCTTTGGAAAAATTCCGATTGGACTATCCGCCAAAAAGTTCTATATCATAAAATTGCCAAAATGGCTGTGAAACTTGACGACGCTCATCAAGATCTTTCAAATGCTATTGTGGTTGCCCAAGCAACATCCACTCAGATGTCCAAGCGCGATCCTTTGAGACCCTACATGGATATTATAGACAACATGTTAGCTCAAATGTTCAGGGAGGACCCATCCTTCAAACCGCAAGGTGGCCGATTAGGTGAAAACGACATGGACTATGATACTGATGAAAAGTCAATGGCAACGCTTAGGCGTCATCTTCGAAGAGCCCGGGAAGAGTATTATCGGTACAAAAGTGAGTATATGACTTATGTTTTGGAGGCCCTTGAACTTGAAGATACTATAAAAAATTATGAACGCCGGAGTTCTACTGGATGGAAATATGTCTCAAGCTTCAGACCTACTCGAACTGGCACATTAGGATCTCCTGTTGATACAATAGAATTCTTATGGCGCTGCATACTAAGAAAAGAAGTTGAGAAACTTTTGGCTGTCATACTTGGTATCATTTCAGTTGCTATTCTTTTAGCGGAGGCAACTCTTCTTCCTCGAGGATTGATCTATCTCTCTTTTCAATTCTCATCAATTCTGTTGGAAAGCAAGAGGTTCTTGTACAGGTATTTGCCTTTGTTCCTTTGCTGTATATGTGCATCTGCACATACTACTCCTTGTTCAAAATTGGTATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LMBR1

Protein Analysis

414

Amino Acids

47.12

Weight (kDa)

8.46

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LMBR1 PF04791 3 - 399 1.4e-63 LMBR1-like membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1101
AccB1I GGYRCC 1 cut(s) 253
AccII CGCG 1 cut(s) 657
AciI CCGC 4 cut(s) 153, 538, 734, 1082
AclWI GGATC 2 cut(s) 653, 982
AcoI YGGCCR 1 cut(s) 742
AcsI RAATTY 2 cut(s) 520, 995
AcuI CTGAAG 1 cut(s) 931
AcyI GRCGYC 1 cut(s) 805
AfaI GTAC 6 cut(s) 65, 113, 229, 321, 842, 1166
AfiI CCNNNNNNNGG 4 cut(s) 37, 162, 265, 740
AflIII ACRYGT 2 cut(s) 322, 693
AgsI TTSAA 8 cut(s) 275, 310, 609, 730, 881, 887, 1127, 1227
AhlI ACTAGT 1 cut(s) 314
AjnI CCWGG 1 cut(s) 116
AluBI AGCT 3 cut(s) 213, 701, 945
AluI AGCT 3 cut(s) 213, 701, 945
Alw26I GTCTC 2 cut(s) 661, 943
AlwI GGATC 2 cut(s) 653, 982
Ama87I CYCGRG 2 cut(s) 822, 1101
AoxI GGCC 4 cut(s) 73, 508, 742, 873
ApeKI GCWGC 2 cut(s) 438, 1008
ApoI RAATTY 2 cut(s) 520, 995
ArsI GACNNNNNNTTYG 2 cut(s) 850, 882
AseI ATTAAT 1 cut(s) 374
AspLEI GCGC 2 cut(s) 657, 1008
AspS9I GGNCC 5 cut(s) 74, 82, 508, 718, 874
AsuC2I CCSGG 3 cut(s) 77, 823, 824
AsuHPI GGTGA 3 cut(s) 94, 283, 764
AsuII TTCGAA 1 cut(s) 814
AvaI CYCGRG 2 cut(s) 822, 1101
AvaII GGWCC 2 cut(s) 82, 718
BaeGI GKGCMC 2 cut(s) 153, 258
BaeI ACNNNNGTAYC 2 cut(s) 832, 865
BanI GGYRCC 1 cut(s) 253
BanII GRGCYC 1 cut(s) 823
BbsI GAAGAC 2 cut(s) 282, 317
BbvI GCAGC 2 cut(s) 425, 995
BccI CCATC 3 cut(s) 143, 730, 921
BciT130I CCWGG 1 cut(s) 118
BclI TGATCA 1 cut(s) 99
BcnI CCSGG 3 cut(s) 77, 823, 824
BcoDI GTCTC 2 cut(s) 661, 943
BcuI ACTAGT 1 cut(s) 314
BfaI CTAG 1 cut(s) 315
BfoI RGCGCY 1 cut(s) 1009
BglI GCCNNNNNGGC 1 cut(s) 572
BglII AGATCT 1 cut(s) 601
BisI GCNGC 2 cut(s) 439, 1009
BlsI GCNGC 2 cut(s) 440, 1010
BmcAI AGTACT 1 cut(s) 65
Bme1390I CCNGG 4 cut(s) 77, 118, 823, 824
Bme18I GGWCC 2 cut(s) 82, 718
BmeT110I CYCGRG 2 cut(s) 822, 1101
BmgT120I GGNCC 5 cut(s) 74, 82, 508, 718, 874
BmiI GGNNCC 3 cut(s) 84, 255, 720
BmrFI CCNGG 4 cut(s) 77, 118, 823, 824
BmsI GCATC 1 cut(s) 1211
BpiI GAAGAC 2 cut(s) 282, 317
Bpu10I CCTNAGC 1 cut(s) 800
Bpu14I TTCGAA 1 cut(s) 814
BpuEI CTTGAG 2 cut(s) 44, 925
BpuMI CCSGG 3 cut(s) 77, 823, 824
BsaHI GRCGYC 1 cut(s) 805
BsaI GGTCTC 1 cut(s) 661
BsaJI CCNNGG 3 cut(s) 36, 432, 822
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 162, 265, 740
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 2 cut(s) 928, 967
BseBI CCWGG 1 cut(s) 118
BseDI CCNNGG 3 cut(s) 36, 432, 822
BseGI GGATG 3 cut(s) 635, 722, 932
BseLI CCNNNNNNNGG 4 cut(s) 37, 162, 265, 740
BseMII CTCAG 1 cut(s) 656
BseNI ACTGG 2 cut(s) 928, 967
BseSI GKGCMC 2 cut(s) 153, 258
BseXI GCAGC 2 cut(s) 425, 995
BseYI CCCAGC 1 cut(s) 281
BsgI GTGCAG 1 cut(s) 1191
Bsh1236I CGCG 1 cut(s) 657
BshFI GGCC 4 cut(s) 75, 510, 744, 875
BshNI GGYRCC 1 cut(s) 253
BsiHKCI CYCGRG 2 cut(s) 822, 1101
BsiSI CCGG 4 cut(s) 72, 77, 823, 913
BslFI GGGAC 2 cut(s) 68, 299
BslI CCNNNNNNNGG 4 cut(s) 37, 162, 265, 740
BsmAI GTCTC 2 cut(s) 661, 943
BsmFI GGGAC 2 cut(s) 68, 299
BsnI GGCC 4 cut(s) 75, 510, 744, 875
Bso31I GGTCTC 1 cut(s) 661
BsoBI CYCGRG 2 cut(s) 822, 1101
Bsp119I TTCGAA 1 cut(s) 814
Bsp1286I GDGCHC 3 cut(s) 153, 258, 823
Bsp1407I TGTACA 2 cut(s) 319, 1164
Bsp143I GATC 6 cut(s) 18, 99, 601, 658, 974, 1110
Bsp19I CCATGG 1 cut(s) 432
BspACI CCGC 4 cut(s) 153, 538, 734, 1082
BspANI GGCC 4 cut(s) 75, 510, 744, 875
BspCNI CTCAG 1 cut(s) 655
BspFNI CGCG 1 cut(s) 657
BspLI GGNNCC 3 cut(s) 84, 255, 720
BspPI GGATC 2 cut(s) 653, 982
BspQI GCTCTTC 1 cut(s) 811
BspT104I TTCGAA 1 cut(s) 814
BspT107I GGYRCC 1 cut(s) 253
BspTNI GGTCTC 1 cut(s) 661
BsrFI RCCGGY 1 cut(s) 71
BsrGI TGTACA 2 cut(s) 319, 1164
BsrI ACTGG 2 cut(s) 928, 967
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 3 cut(s) 36, 432, 822
BssMI GATC 6 cut(s) 18, 99, 601, 658, 974, 1110
BssNI GRCGYC 1 cut(s) 805
BssT1I CCWWGG 2 cut(s) 36, 432
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 4 cut(s) 106, 250, 295, 401
Bst6I CTCTTC 3 cut(s) 811, 822, 1098
BstACI GRCGYC 1 cut(s) 805
BstAUI TGTACA 2 cut(s) 319, 1164
BstBI TTCGAA 1 cut(s) 814
BstC8I GCNNGC 2 cut(s) 73, 153
BstDEI CTNAG 3 cut(s) 642, 800, 1016
BstDSI CCRYGG 1 cut(s) 432
BstENI CCTNNNNNAGG 1 cut(s) 263
BstF5I GGATG 3 cut(s) 635, 722, 932
BstFNI CGCG 1 cut(s) 657
BstH2I RGCGCY 1 cut(s) 1009
BstHHI GCGC 2 cut(s) 657, 1008
BstKTI GATC 6 cut(s) 21, 102, 604, 661, 977, 1113
BstMAI GTCTC 2 cut(s) 661, 943
BstMBI GATC 6 cut(s) 18, 99, 601, 658, 974, 1110
BstMWI GCNNNNNNNGC 1 cut(s) 572
BstNI CCWGG 1 cut(s) 118
BstNSI RCATGY 2 cut(s) 326, 697
BstSCI CCNGG 4 cut(s) 75, 116, 821, 822
BstSLI GKGCMC 2 cut(s) 153, 258
BstUI CGCG 1 cut(s) 657
BstV1I GCAGC 2 cut(s) 425, 995
BstV2I GAAGAC 2 cut(s) 282, 317
BstX2I RGATCY 2 cut(s) 601, 974
BstYI RGATCY 2 cut(s) 601, 974
BsuRI GGCC 4 cut(s) 75, 510, 744, 875
BtgI CCRYGG 1 cut(s) 432
BtsCI GGATG 3 cut(s) 635, 722, 932
Cac8I GCNNGC 2 cut(s) 73, 153
CfoI GCGC 2 cut(s) 657, 1008
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 5 cut(s) 74, 82, 508, 718, 874
Cfr9I CCCGGG 1 cut(s) 822
CseI GACGC 3 cut(s) 287, 599, 794
CsiI ACCWGGT 1 cut(s) 116
Csp6I GTAC 6 cut(s) 64, 112, 228, 320, 841, 1165
CviAII CATG 6 cut(s) 43, 323, 433, 676, 694, 763
CviQI GTAC 6 cut(s) 64, 112, 228, 320, 841, 1165
DdeI CTNAG 3 cut(s) 642, 800, 1016
DpnI GATC 6 cut(s) 20, 101, 603, 660, 976, 1112
DpnII GATC 6 cut(s) 18, 99, 601, 658, 974, 1110
EaeI YGGCCR 1 cut(s) 742
Eam1104I CTCTTC 3 cut(s) 811, 822, 1098
EarI CTCTTC 3 cut(s) 811, 822, 1098
EciI GGCGGA 1 cut(s) 527
Eco130I CCWWGG 2 cut(s) 36, 432
Eco24I GRGCYC 1 cut(s) 823
Eco31I GGTCTC 1 cut(s) 661
Eco47I GGWCC 2 cut(s) 82, 718
Eco57I CTGAAG 1 cut(s) 931
Eco88I CYCGRG 2 cut(s) 822, 1101
EcoNI CCTNNNNNAGG 1 cut(s) 263
EcoO109I RGGNCCY 4 cut(s) 82, 508, 718, 874
EcoRI GAATTC 1 cut(s) 995
EcoRII CCWGG 1 cut(s) 116
EcoT14I CCWWGG 2 cut(s) 36, 432
EcoT38I GRGCYC 1 cut(s) 823
ErhI CCWWGG 2 cut(s) 36, 432
FaeI CATG 6 cut(s) 46, 326, 436, 679, 697, 766
FalI AAGNNNNNCTT 2 cut(s) 1145, 1177
FaqI GGGAC 2 cut(s) 68, 299
FatI CATG 6 cut(s) 42, 322, 432, 675, 693, 762
FauI CCCGC 1 cut(s) 160
FbaI TGATCA 1 cut(s) 99
Fnu4HI GCNGC 2 cut(s) 439, 1009
FokI GGATG 3 cut(s) 622, 709, 939
FriOI GRGCYC 1 cut(s) 823
Fsp4HI GCNGC 2 cut(s) 439, 1009
FspBI CTAG 1 cut(s) 315
GlaI GCGC 2 cut(s) 656, 1007
GluI GCNGC 2 cut(s) 439, 1009
GsaI CCCAGC 1 cut(s) 285
HaeII RGCGCY 1 cut(s) 1009
HaeIII GGCC 4 cut(s) 75, 510, 744, 875
HapII CCGG 4 cut(s) 72, 77, 823, 913
HgaI GACGC 3 cut(s) 287, 599, 794
HhaI GCGC 2 cut(s) 657, 1008
Hin1I GRCGYC 1 cut(s) 805
Hin1II CATG 6 cut(s) 46, 326, 436, 679, 697, 766
Hin6I GCGC 2 cut(s) 655, 1006
HinP1I GCGC 2 cut(s) 655, 1006
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HindIII AAGCTT 1 cut(s) 943
HpaI GTTAAC 1 cut(s) 328
HpaII CCGG 4 cut(s) 72, 77, 823, 913
HphI GGTGA 3 cut(s) 94, 283, 764
Hpy166II GTNNAC 3 cut(s) 114, 320, 328
Hpy188I TCNGA 3 cut(s) 526, 645, 950
Hpy188III TCNNGA 3 cut(s) 163, 502, 599
Hpy8I GTNNAC 3 cut(s) 114, 320, 328
Hpy99I CGWCG 3 cut(s) 110, 172, 593
HpyAV CCTTC 2 cut(s) 499, 736
HpyCH4III ACNGT 4 cut(s) 106, 250, 295, 401
HpyCH4V TGCA 5 cut(s) 394, 470, 1011, 1202, 1208
HpyF10VI GCNNNNNNNGC 1 cut(s) 572
HpyF3I CTNAG 3 cut(s) 642, 800, 1016
Hsp92I GRCGYC 1 cut(s) 805
Hsp92II CATG 6 cut(s) 46, 326, 436, 679, 697, 766
HspAI GCGC 2 cut(s) 655, 1006
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
Ksp22I TGATCA 1 cut(s) 99
KspAI GTTAAC 1 cut(s) 328
Kzo9I GATC 6 cut(s) 18, 99, 601, 658, 974, 1110
LguI GCTCTTC 1 cut(s) 811
LmnI GCTCC 1 cut(s) 210
Lsp1109I GCAGC 2 cut(s) 425, 995
LweI GCATC 1 cut(s) 1211
MabI ACCWGGT 1 cut(s) 116
MaeI CTAG 1 cut(s) 315
MaeIII GTNAC 3 cut(s) 295, 401, 460
MalI GATC 6 cut(s) 20, 101, 603, 660, 976, 1112
MboI GATC 6 cut(s) 18, 99, 601, 658, 974, 1110
MflI RGATCY 2 cut(s) 601, 974
MhlI GDGCHC 3 cut(s) 153, 258, 823
MluCI AATT 9 cut(s) 345, 375, 520, 561, 901, 995, 1127, 1137, 1229
MmeI TCCRAC 1 cut(s) 1125
MroNI GCCGGC 1 cut(s) 71
MseI TTAA 2 cut(s) 327, 374
MslI CAYNNNNRTG 2 cut(s) 389, 644
MspI CCGG 4 cut(s) 72, 77, 823, 913
MspR9I CCNGG 4 cut(s) 77, 118, 823, 824
MvaI CCWGG 1 cut(s) 118
MvnI CGCG 1 cut(s) 657
MwoI GCNNNNNNNGC 1 cut(s) 572
NaeI GCCGGC 1 cut(s) 73
NciI CCSGG 3 cut(s) 77, 823, 824
NcoI CCATGG 1 cut(s) 432
NdeII GATC 6 cut(s) 18, 99, 601, 658, 974, 1110
NgoMIV GCCGGC 1 cut(s) 71
NlaIII CATG 6 cut(s) 46, 326, 436, 679, 697, 766
NlaIV GGNNCC 3 cut(s) 84, 255, 720
NmuCI GTSAC 1 cut(s) 295
NspI RCATGY 2 cut(s) 326, 697
NspV TTCGAA 1 cut(s) 814
PaeR7I CTCGAG 1 cut(s) 1101
PciI ACATGT 2 cut(s) 322, 693
PciSI GCTCTTC 1 cut(s) 811
PdiI GCCGGC 1 cut(s) 73
PkrI GCNGC 2 cut(s) 440, 1010
PpuMI RGGWCCY 2 cut(s) 82, 718
PscI ACATGT 2 cut(s) 322, 693
PshBI ATTAAT 1 cut(s) 374
Psp5II RGGWCCY 2 cut(s) 82, 718
Psp6I CCWGG 1 cut(s) 116
PspFI CCCAGC 1 cut(s) 281
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 3 cut(s) 84, 255, 720
PspPI GGNCC 5 cut(s) 74, 82, 508, 718, 874
PspPPI RGGWCCY 2 cut(s) 82, 718
PspXI VCTCGAGB 1 cut(s) 1101
PsrI GAACNNNNNNTAC 2 cut(s) 104, 136
PsuI RGATCY 2 cut(s) 601, 974
RsaI GTAC 6 cut(s) 65, 113, 229, 321, 842, 1166
RsaNI GTAC 6 cut(s) 64, 112, 228, 320, 841, 1165
RseI CAYNNNNRTG 2 cut(s) 389, 644
SapI GCTCTTC 1 cut(s) 811
SaqAI TTAA 2 cut(s) 327, 374
SatI GCNGC 2 cut(s) 439, 1009
Sau3AI GATC 6 cut(s) 18, 99, 601, 658, 974, 1110
Sau96I GGNCC 5 cut(s) 74, 82, 508, 718, 874
ScaI AGTACT 1 cut(s) 65
ScrFI CCNGG 4 cut(s) 77, 118, 823, 824
SduI GDGCHC 3 cut(s) 153, 258, 823
SexAI ACCWGGT 1 cut(s) 116
SfaNI GCATC 1 cut(s) 1211
Sfr274I CTCGAG 1 cut(s) 1101
SfuI TTCGAA 1 cut(s) 814
SinI GGWCC 2 cut(s) 82, 718
SlaI CTCGAG 1 cut(s) 1101
SmaI CCCGGG 1 cut(s) 824
SmiMI CAYNNNNRTG 2 cut(s) 389, 644
SmlI CTYRAG 3 cut(s) 59, 940, 1101
SmoI CTYRAG 3 cut(s) 59, 940, 1101
SpeI ACTAGT 1 cut(s) 314
Sse9I AATT 9 cut(s) 345, 375, 520, 561, 901, 995, 1127, 1137, 1229
SsiI CCGC 4 cut(s) 153, 538, 734, 1082
SspMI CTAG 1 cut(s) 315
StyD4I CCNGG 4 cut(s) 75, 116, 821, 822
StyI CCWWGG 2 cut(s) 36, 432
TaaI ACNGT 4 cut(s) 106, 250, 295, 401
TaqI TCGA 4 cut(s) 443, 814, 958, 1102
TasI AATT 9 cut(s) 345, 375, 520, 561, 901, 995, 1127, 1137, 1229
TatI WGTACW 4 cut(s) 63, 227, 319, 1164
Tru1I TTAA 2 cut(s) 327, 374
Tru9I TTAA 2 cut(s) 327, 374
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 2 cut(s) 438, 1008
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 2 cut(s) 795, 921
TspMI CCCGGG 1 cut(s) 822
VpaK11BI GGWCC 2 cut(s) 82, 718
VspI ATTAAT 1 cut(s) 374
XagI CCTNNNNNAGG 1 cut(s) 263
XapI RAATTY 2 cut(s) 520, 995
XceI RCATGY 2 cut(s) 326, 697
XcmI CCANNNNNNNNNTGG 1 cut(s) 185
XhoI CTCGAG 1 cut(s) 1101
XmaI CCCGGG 1 cut(s) 822
XspI CTAG 1 cut(s) 315
ZrmI AGTACT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.