pycom01g10420

Ubiquitin-conjugating enzyme E2 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
N/A
Physical Location & Seq
Forward (+)
11210102 .. 11213184
3083 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 738 bp
ATGAAAAAATTAAAAAAACCAAACCAAACCAAATCAAAATCTACAAAATTTCGGTTTGATTTCGATTTTGACAAAAAAAAAATTGAACGGAGTAAAACCGAACCCACCCTTACTTTCACATCTATCGGGCAGATAGTGACAAAAATACATTATTATTATTCCGTCCCTGCATATCATCTTCTTATTATTGGACACTTTGTCAAGTTCCTTCCTCTTCTCTCTCTCTTCTCCTCTCTGTCAAAGCAACGCCCCCTTCCTTCCTTAGATCCGTACGATCTGCGAGGTCCATCCATGGCTTCCAAACGGATCTTGAAGGAGCTTAACGATCTCCAGAAGGACCCCCCGACCTCTTGCAGCGCAGGGCCTGTTGCTGAGGACATGTTCCATTGGCAAGCAACGATAATGGGCCCTCCAGATAGCCCTTATGCAGGGGGTGTCTTTCTAGTTACCATTCATTTTCCTCCTGATTATCCATTCAAACCACCTAAGGTTGCCTTTAGGACTAAGGTATTTCATCCCAATATCAACAGCAATGGAAGTATCTGCCTTGACATTTTGAAAGAGCAGTGGAGCCCTGCTCTCACCATATCTAAGGTATTGCTATCCATCTGTTCCCTGTTGACGGACCCAAACCCAGATGATCGCTTGGTGCCGGAAATTGCTCATATGTACAAGACAGACCGGTCAAAGTACGAGACAACCGCAAGGAGCTGGACCCAGAAGTATGCTATGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

246

Amino Acids

27.9

Weight (kDa)

9.52

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 102 - 238 6.8e-53 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 682
AccB1I GGYRCC 1 cut(s) 649
AciI CCGC 1 cut(s) 702
AclWI GGATC 2 cut(s) 260, 314
AcsI RAATTY 1 cut(s) 47
AfaI GTAC 3 cut(s) 272, 671, 692
AfiI CCNNNNNNNGG 2 cut(s) 428, 622
AflIII ACRYGT 1 cut(s) 378
AgeI ACCGGT 1 cut(s) 681
AgsI TTSAA 4 cut(s) 86, 313, 478, 559
AhdI GACNNNNNGTC 1 cut(s) 197
AluBI AGCT 2 cut(s) 319, 711
AluI AGCT 2 cut(s) 319, 711
Alw26I GTCTC 1 cut(s) 689
AlwI GGATC 2 cut(s) 260, 314
AlwNI CAGNNNCTG 1 cut(s) 365
AoxI GGCC 2 cut(s) 362, 406
ApaI GGGCCC 1 cut(s) 410
ApeKI GCWGC 1 cut(s) 354
ApoI RAATTY 1 cut(s) 47
AsiGI ACCGGT 1 cut(s) 681
AspLEI GCGC 1 cut(s) 359
AspS9I GGNCC 7 cut(s) 284, 337, 362, 406, 407, 625, 714
AsuHPI GGTGA 1 cut(s) 574
AvaII GGWCC 4 cut(s) 284, 337, 625, 714
AxyI CCTNAGG 1 cut(s) 486
BaeGI GKGCMC 1 cut(s) 410
BanI GGYRCC 1 cut(s) 649
BanII GRGCYC 2 cut(s) 410, 575
BbvCI CCTCAGC 1 cut(s) 372
BbvI GCAGC 1 cut(s) 366
BccI CCATC 2 cut(s) 295, 614
BcoDI GTCTC 1 cut(s) 689
BfaI CTAG 2 cut(s) 443, 736
BisI GCNGC 1 cut(s) 355
BlsI GCNGC 1 cut(s) 356
Bme18I GGWCC 4 cut(s) 284, 337, 625, 714
BmeRI GACNNNNNGTC 1 cut(s) 197
BmgT120I GGNCC 7 cut(s) 284, 337, 362, 406, 407, 625, 714
BmiI GGNNCC 6 cut(s) 339, 408, 572, 627, 651, 716
BplI GAGNNNNNCTC 2 cut(s) 562, 594
BpmI CTGGAG 2 cut(s) 314, 396
Bpu10I CCTNAGC 1 cut(s) 372
BsaJI CCNNGG 1 cut(s) 291
BsaWI WCCGGW 1 cut(s) 681
Bsc4I CCNNNNNNNGG 2 cut(s) 428, 622
Bse118I RCCGGY 1 cut(s) 681
Bse21I CCTNAGG 1 cut(s) 486
Bse3DI GCAATG 1 cut(s) 538
BseDI CCNNGG 1 cut(s) 291
BseGI GGATG 2 cut(s) 287, 514
BseLI CCNNNNNNNGG 2 cut(s) 428, 622
BseMI GCAATG 1 cut(s) 538
BseMII CTCAG 1 cut(s) 363
BseRI GAGGAG 1 cut(s) 220
BseSI GKGCMC 1 cut(s) 410
BseXI GCAGC 1 cut(s) 366
BshFI GGCC 2 cut(s) 364, 408
BshNI GGYRCC 1 cut(s) 649
BshTI ACCGGT 1 cut(s) 681
BsiSI CCGG 2 cut(s) 653, 682
BsiWI CGTACG 1 cut(s) 270
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 2 cut(s) 428, 622
BsmAI GTCTC 1 cut(s) 689
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 2 cut(s) 364, 408
Bsp120I GGGCCC 1 cut(s) 406
Bsp1286I GDGCHC 2 cut(s) 410, 575
Bsp1407I TGTACA 1 cut(s) 669
Bsp143I GATC 5 cut(s) 265, 274, 306, 325, 640
Bsp19I CCATGG 1 cut(s) 291
BspACI CCGC 1 cut(s) 702
BspANI GGCC 2 cut(s) 364, 408
BspCNI CTCAG 1 cut(s) 364
BspLI GGNNCC 6 cut(s) 339, 408, 572, 627, 651, 716
BspPI GGATC 2 cut(s) 260, 314
BspT107I GGYRCC 1 cut(s) 649
BsrDI GCAATG 1 cut(s) 538
BsrFI RCCGGY 1 cut(s) 681
BsrGI TGTACA 1 cut(s) 669
BssAI RCCGGY 1 cut(s) 681
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 5 cut(s) 265, 274, 306, 325, 640
BssT1I CCWWGG 1 cut(s) 291
Bst6I CTCTTC 2 cut(s) 219, 230
BstAUI TGTACA 1 cut(s) 669
BstC8I GCNNGC 1 cut(s) 393
BstDEI CTNAG 5 cut(s) 262, 372, 486, 504, 591
BstDSI CCRYGG 1 cut(s) 291
BstENI CCTNNNNNAGG 1 cut(s) 426
BstF5I GGATG 2 cut(s) 287, 514
BstHHI GCGC 1 cut(s) 359
BstKTI GATC 5 cut(s) 268, 277, 309, 328, 643
BstMAI GTCTC 1 cut(s) 689
BstMBI GATC 5 cut(s) 265, 274, 306, 325, 640
BstNSI RCATGY 1 cut(s) 382
BstSLI GKGCMC 1 cut(s) 410
BstV1I GCAGC 1 cut(s) 366
BstX2I RGATCY 2 cut(s) 265, 306
BstYI RGATCY 2 cut(s) 265, 306
Bsu36I CCTNAGG 1 cut(s) 486
BsuRI GGCC 2 cut(s) 364, 408
BtgI CCRYGG 1 cut(s) 291
BtsCI GGATG 2 cut(s) 287, 514
BtsI GCAGTG 1 cut(s) 572
BtsIMutI CAGTG 1 cut(s) 572
Cac8I GCNNGC 1 cut(s) 393
CaiI CAGNNNCTG 1 cut(s) 365
CfoI GCGC 1 cut(s) 359
Cfr10I RCCGGY 1 cut(s) 681
Cfr13I GGNCC 7 cut(s) 284, 337, 362, 406, 407, 625, 714
Csp6I GTAC 3 cut(s) 271, 670, 691
CspAI ACCGGT 1 cut(s) 681
CviAII CATG 2 cut(s) 292, 379
CviJI RGCY 8 cut(s) 296, 319, 364, 408, 420, 573, 711, 735
CviKI_1 RGCY 8 cut(s) 296, 319, 364, 408, 420, 573, 711, 735
CviQI GTAC 3 cut(s) 271, 670, 691
DdeI CTNAG 5 cut(s) 262, 372, 486, 504, 591
DpnI GATC 5 cut(s) 267, 276, 308, 327, 642
DpnII GATC 5 cut(s) 265, 274, 306, 325, 640
DrdI GACNNNNNNGTC 1 cut(s) 682
DriI GACNNNNNGTC 1 cut(s) 197
DseDI GACNNNNNNGTC 1 cut(s) 682
Eam1104I CTCTTC 2 cut(s) 219, 230
Eam1105I GACNNNNNGTC 1 cut(s) 197
EarI CTCTTC 2 cut(s) 219, 230
Eco130I CCWWGG 1 cut(s) 291
Eco24I GRGCYC 2 cut(s) 410, 575
Eco47I GGWCC 4 cut(s) 284, 337, 625, 714
Eco81I CCTNAGG 1 cut(s) 486
EcoNI CCTNNNNNAGG 1 cut(s) 426
EcoO109I RGGNCCY 3 cut(s) 337, 362, 407
EcoT14I CCWWGG 1 cut(s) 291
EcoT38I GRGCYC 2 cut(s) 410, 575
ErhI CCWWGG 1 cut(s) 291
FaeI CATG 2 cut(s) 295, 382
FaiI YATR 9 cut(s) 172, 293, 380, 426, 587, 666, 668, 726, 731
FalI AAGNNNNNCTT 2 cut(s) 479, 511
FaqI GGGAC 1 cut(s) 149
FatI CATG 2 cut(s) 291, 378
FauNDI CATATG 1 cut(s) 666
Fnu4HI GCNGC 1 cut(s) 355
FokI GGATG 2 cut(s) 274, 501
FriOI GRGCYC 2 cut(s) 410, 575
Fsp4HI GCNGC 1 cut(s) 355
FspBI CTAG 2 cut(s) 443, 736
GlaI GCGC 1 cut(s) 358
GluI GCNGC 1 cut(s) 355
GsuI CTGGAG 2 cut(s) 314, 396
HaeIII GGCC 2 cut(s) 364, 408
HapII CCGG 2 cut(s) 653, 682
HhaI GCGC 1 cut(s) 359
Hin1II CATG 2 cut(s) 295, 382
Hin6I GCGC 1 cut(s) 357
HinP1I GCGC 1 cut(s) 357
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HpaII CCGG 2 cut(s) 653, 682
HphI GGTGA 1 cut(s) 574
Hpy166II GTNNAC 1 cut(s) 621
Hpy188III TCNNGA 4 cut(s) 310, 331, 413, 464
Hpy8I GTNNAC 1 cut(s) 621
HpyAV CCTTC 5 cut(s) 218, 263, 267, 307, 328
HpyCH4V TGCA 3 cut(s) 170, 354, 428
HpyF3I CTNAG 5 cut(s) 262, 372, 486, 504, 591
Hsp92II CATG 2 cut(s) 295, 382
HspAI GCGC 1 cut(s) 357
Kzo9I GATC 5 cut(s) 265, 274, 306, 325, 640
LmnI GCTCC 3 cut(s) 316, 570, 708
Lsp1109I GCAGC 1 cut(s) 366
MaeI CTAG 2 cut(s) 443, 736
MaeIII GTNAC 2 cut(s) 136, 445
MalI GATC 5 cut(s) 267, 276, 308, 327, 642
MboI GATC 5 cut(s) 265, 274, 306, 325, 640
MboII GAAGA 3 cut(s) 170, 206, 217
MflI RGATCY 2 cut(s) 265, 306
MhlI GDGCHC 2 cut(s) 410, 575
MluCI AATT 4 cut(s) 8, 47, 81, 657
MnlI CCTC 7 cut(s) 222, 241, 275, 358, 367, 420, 471
MseI TTAA 2 cut(s) 11, 321
MspI CCGG 2 cut(s) 653, 682
NcoI CCATGG 1 cut(s) 291
NdeI CATATG 1 cut(s) 666
NdeII GATC 5 cut(s) 265, 274, 306, 325, 640
NlaIII CATG 2 cut(s) 295, 382
NlaIV GGNNCC 6 cut(s) 339, 408, 572, 627, 651, 716
NmuCI GTSAC 1 cut(s) 136
NspI RCATGY 1 cut(s) 382
PciI ACATGT 1 cut(s) 378
Pfl23II CGTACG 1 cut(s) 270
PinAI ACCGGT 1 cut(s) 681
PkrI GCNGC 1 cut(s) 356
PpuMI RGGWCCY 1 cut(s) 337
PscI ACATGT 1 cut(s) 378
Psp5II RGGWCCY 1 cut(s) 337
PspLI CGTACG 1 cut(s) 270
PspN4I GGNNCC 6 cut(s) 339, 408, 572, 627, 651, 716
PspOMI GGGCCC 1 cut(s) 406
PspPI GGNCC 7 cut(s) 284, 337, 362, 406, 407, 625, 714
PspPPI RGGWCCY 1 cut(s) 337
PstNI CAGNNNCTG 1 cut(s) 365
PsuI RGATCY 2 cut(s) 265, 306
RsaI GTAC 3 cut(s) 272, 671, 692
RsaNI GTAC 3 cut(s) 271, 670, 691
SaqAI TTAA 2 cut(s) 11, 321
SatI GCNGC 1 cut(s) 355
Sau3AI GATC 5 cut(s) 265, 274, 306, 325, 640
Sau96I GGNCC 7 cut(s) 284, 337, 362, 406, 407, 625, 714
SduI GDGCHC 2 cut(s) 410, 575
SetI ASST 8 cut(s) 286, 321, 350, 487, 492, 510, 597, 713
SinI GGWCC 4 cut(s) 284, 337, 625, 714
Sse9I AATT 4 cut(s) 8, 47, 81, 657
SsiI CCGC 1 cut(s) 702
SspMI CTAG 2 cut(s) 443, 736
StyI CCWWGG 1 cut(s) 291
TaqI TCGA 1 cut(s) 63
TasI AATT 4 cut(s) 8, 47, 81, 657
TatI WGTACW 1 cut(s) 669
Tru1I TTAA 2 cut(s) 11, 321
Tru9I TTAA 2 cut(s) 11, 321
TscAI CASTG 1 cut(s) 572
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 1 cut(s) 354
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 3 cut(s) 17, 443, 503
TspGWI ACGGA 5 cut(s) 103, 151, 258, 319, 638
TspRI CASTG 1 cut(s) 572
VpaK11BI GGWCC 4 cut(s) 284, 337, 625, 714
XagI CCTNNNNNAGG 1 cut(s) 426
XapI RAATTY 1 cut(s) 47
XceI RCATGY 1 cut(s) 382
XspI CTAG 2 cut(s) 443, 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.