pycom01g12340

regulation of response to stimulus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
N/A
Physical Location & Seq
Forward (+)
13096548 .. 13099548
3001 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1752 bp
ATGTACAAACATTACACTTTAACGAAGACTCTGGAATATTACAAAATAATGACGAAAGTAATTTTCGCAAATAACCAAAGAAGTTTCCATTATCACGTCCTTGAACTTGATGAACACACCCTATGCCACATCATCAACATAATACAACCCCCTCCTCTTAGTACCACGCCCAATGATTTCTTTTGCACAACACAGTTTAATTATTCGAGTAGGAGTGTAGGAGTGAGTGCAATCGAGCTCGCCCTTGTAATTTGGGCCACACCGCCATTAGCAGTGACAATTACTATCTTGAGGTGGTGGTATTTGGCTCCGAAGTCAATAATCCAACTTGTATCAAGATCAATAGATACAATTAAGGCTGTGCTTGTATTACCTGAATCGTTGGACTGGTTGTCTGTTGGTGGGCGCGACGTGCCCATAATTCCTGCGCTGGTTTTCCTGATGTTTGAAACTGGCTCGGCATGTGTGTCAGCGTACGGTCCAGCGTGTGTAACAACCTCCATGGAAGAGACTGCTGGCAAGACACTGGGCTTTAGTTTTCCTAGGTCTTGTTGCTTTGTAAACTTTCTTTTGGGTTTTAGAGACACCATGATACGGAGAAATTCTATTTTTGTTTACGCTTCGGAAAACAGAAAAATTGGCGAATTCAAGCTCGTACTTCTTGTACCATATGTTATCAGAATTTGGAACGCATATCAGATTCTCACGTCGAGCGATAAGTTTCTCTTATTGATTTCTTTTTTAAAAGGAAAACTTGTATGTTATAGAGCAAAGCATCAGGATTATATTCAAAGGATAGGGGAAAACCATGATACAGAAGGCTTACAAACAATAGCAAAGTTTCCAACTTTTGTAGATAACATGTCAGAGCATCTGAATAAAAACGATTTCCCTATGAATGTGTGCCCCACTGATATGCAAGATTCTGCCATGAGAAAGGCTAGGAATTCAATTAATGCTTTCTTAGCCAGTAGTCTATACATATTTAGGCACACTTTTCAGCCTCACACACCCTGCTTAATAATGTATACTGGTGAATTGCCTCCCTACATTTACGTCACAAGAATTCTTATAAATTGGGTTCTTCTCAAGCTGCTAGACAACCCAAAGTTTTACACAAAACTGATACCATGGGGATTTACAAGTTTATTTCACCACTTCTCTTCTTCTGATATTTCTGCATACATGCCTTTGTTCTTAACTCATGTCTTAGTTTGGCCTTCGCCAGATCAAACGAGTCAGATAAACTGTCATGACTTCATGGAACGTAACAATCCATTTTTGCCAATGGCACGGTGTTACCTGTGGTCACCGCCACAAGAGGATCACAATCTGAGCTCCTTGAAGCTTGGAGGCTCCATATCACGACATGTTGAAAATTTGAGTTTCCTCAGAGTTGGTTGTCTGCGCAGGCTGCGACATTTGGCGTTGTATAATAATTCACTGAGTGGAGAAATTCCCACTAATTTTTCAGGTTGTTCTGAACTCAACTACATATATCTTGACCACAATTTGTTAGAAGGTAGTATCCCCAAGGAGCTTGGCACCTTGTCAAAGCTAATCGAATTCACTAGTAAATACGCACGCGCGTTGCTACGGGAACCAGCTTTTACGTACCCAAGAGAGTATCTGAGTTTCATGGTAGAAGCTGCTGAACATAGTTGGACACAAAGGCCTATCAATCAATCGACAAATCAGAACCTACACAGAACCAGACGGATCGATTCCATCAACTATAGTACACAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

584

Amino Acids

67.14

Weight (kDa)

8.73

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1073
Acc16I TGCGCA 1 cut(s) 1409
AccB1I GGYRCC 1 cut(s) 1544
AccI GTMKAC 1 cut(s) 1028
AccII CGCG 3 cut(s) 408, 1587, 1589
AciI CCGC 2 cut(s) 263, 1313
AclWI GGATC 2 cut(s) 1332, 1727
AcsI RAATTY 8 cut(s) 601, 644, 681, 946, 1065, 1378, 1455, 1565
AdeI CACNNNGTG 1 cut(s) 1448
AfaI GTAC 7 cut(s) 5, 163, 476, 657, 666, 1616, 1741
AfiI CCNNNNNNNGG 1 cut(s) 594
AflIII ACRYGT 2 cut(s) 861, 1369
AgsI TTSAA 7 cut(s) 104, 449, 649, 791, 951, 1345, 1376
AhdI GACNNNNNGTC 1 cut(s) 391
AhlI ACTAGT 1 cut(s) 1571
AjiI CACGTC 3 cut(s) 97, 412, 708
AluBI AGCT 9 cut(s) 238, 652, 1093, 1338, 1348, 1540, 1558, 1607, 1649
AluI AGCT 9 cut(s) 238, 652, 1093, 1338, 1348, 1540, 1558, 1607, 1649
Alw21I GWGCWC 2 cut(s) 240, 1340
Alw26I GTCTC 2 cut(s) 503, 576
AlwI GGATC 2 cut(s) 1332, 1727
AoxI GGCC 3 cut(s) 255, 1217, 1673
ApeKI GCWGC 3 cut(s) 1093, 1414, 1649
ApoI RAATTY 8 cut(s) 601, 644, 681, 946, 1065, 1378, 1455, 1565
AseI ATTAAT 1 cut(s) 954
AspA2I CCTAGG 1 cut(s) 542
AspLEI GCGC 4 cut(s) 408, 430, 1410, 1589
AspS9I GGNCC 2 cut(s) 255, 479
AsuHPI GGTGA 3 cut(s) 1046, 1145, 1302
AvaII GGWCC 1 cut(s) 479
AvrII CCTAGG 1 cut(s) 542
BaeGI GKGCMC 2 cut(s) 417, 908
BanI GGYRCC 1 cut(s) 1544
BanII GRGCYC 2 cut(s) 240, 1340
BbsI GAAGAC 1 cut(s) 32
Bbv12I GWGCWC 2 cut(s) 240, 1340
BbvI GCAGC 3 cut(s) 1080, 1401, 1636
BccI CCATC 1 cut(s) 1736
BciVI GTATCC 1 cut(s) 1538
BcoDI GTCTC 2 cut(s) 503, 576
BcuI ACTAGT 1 cut(s) 1571
BfaI CTAG 4 cut(s) 543, 942, 1097, 1572
BfmI CTRYAG 1 cut(s) 1735
BfuI GTATCC 1 cut(s) 1538
BisI GCNGC 3 cut(s) 1094, 1415, 1650
BlnI CCTAGG 1 cut(s) 542
BlsI GCNGC 3 cut(s) 1095, 1416, 1651
Bme18I GGWCC 1 cut(s) 479
BmeRI GACNNNNNGTC 1 cut(s) 391
BmgBI CACGTC 3 cut(s) 97, 412, 708
BmgT120I GGNCC 2 cut(s) 255, 479
BmiI GGNNCC 4 cut(s) 309, 1357, 1546, 1602
BmrI ACTGGG 1 cut(s) 536
BmsI GCATC 2 cut(s) 784, 880
BmuI ACTGGG 1 cut(s) 536
BpiI GAAGAC 1 cut(s) 32
BpuEI CTTGAG 2 cut(s) 310, 1073
Bsa29I ATCGAT 1 cut(s) 1722
BsaAI YACGTR 1 cut(s) 1614
BsaBI GATNNNNATC 1 cut(s) 1329
BsaJI CCNNGG 4 cut(s) 501, 542, 1130, 1533
Bsc4I CCNNNNNNNGG 1 cut(s) 594
Bse1I ACTGG 5 cut(s) 392, 457, 531, 969, 1036
Bse8I GATNNNNATC 1 cut(s) 1329
BseCI ATCGAT 1 cut(s) 1722
BseDI CCNNGG 4 cut(s) 501, 542, 1130, 1533
BseJI GATNNNNATC 1 cut(s) 1329
BseLI CCNNNNNNNGG 1 cut(s) 594
BseMII CTCAG 4 cut(s) 1325, 1405, 1436, 1622
BseNI ACTGG 5 cut(s) 392, 457, 531, 969, 1036
BseRI GAGGAG 1 cut(s) 144
BseSI GKGCMC 2 cut(s) 417, 908
BseXI GCAGC 3 cut(s) 1080, 1401, 1636
Bsh1236I CGCG 3 cut(s) 408, 1587, 1589
BshFI GGCC 3 cut(s) 257, 1219, 1675
BshNI GGYRCC 1 cut(s) 1544
BshVI ATCGAT 1 cut(s) 1722
BsiHKAI GWGCWC 2 cut(s) 240, 1340
BsiWI CGTACG 1 cut(s) 474
BslI CCNNNNNNNGG 1 cut(s) 594
BsmAI GTCTC 2 cut(s) 503, 576
BsnI GGCC 3 cut(s) 257, 1219, 1675
Bsp1286I GDGCHC 4 cut(s) 240, 417, 908, 1340
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 4 cut(s) 338, 1228, 1324, 1719
Bsp19I CCATGG 2 cut(s) 501, 1130
BspACI CCGC 2 cut(s) 263, 1313
BspANI GGCC 3 cut(s) 257, 1219, 1675
BspCNI CTCAG 4 cut(s) 1326, 1404, 1437, 1623
BspDI ATCGAT 1 cut(s) 1722
BspFNI CGCG 3 cut(s) 408, 1587, 1589
BspHI TCATGA 1 cut(s) 1252
BspLI GGNNCC 4 cut(s) 309, 1357, 1546, 1602
BspPI GGATC 2 cut(s) 1332, 1727
BspT107I GGYRCC 1 cut(s) 1544
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 5 cut(s) 392, 457, 531, 969, 1036
BssECI CCNNGG 4 cut(s) 501, 542, 1130, 1533
BssMI GATC 4 cut(s) 338, 1228, 1324, 1719
BssNAI GTATAC 1 cut(s) 1029
BssT1I CCWWGG 4 cut(s) 501, 542, 1130, 1533
Bst1107I GTATAC 1 cut(s) 1029
Bst4CI ACNGT 4 cut(s) 195, 479, 1250, 1296
Bst6I CTCTTC 2 cut(s) 501, 1168
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 1614
BstC8I GCNNGC 4 cut(s) 240, 517, 1412, 1585
BstDEI CTNAG 7 cut(s) 158, 964, 1210, 1334, 1391, 1445, 1631
BstDSI CCRYGG 2 cut(s) 501, 1130
BstEII GGTNACC 1 cut(s) 1308
BstFNI CGCG 3 cut(s) 408, 1587, 1589
BstHHI GCGC 4 cut(s) 408, 430, 1410, 1589
BstKTI GATC 4 cut(s) 341, 1231, 1327, 1722
BstMAI GTCTC 2 cut(s) 503, 576
BstMBI GATC 4 cut(s) 338, 1228, 1324, 1719
BstMWI GCNNNNNNNGC 3 cut(s) 412, 965, 1414
BstNSI RCATGY 4 cut(s) 465, 865, 1189, 1373
BstPI GGTNACC 1 cut(s) 1308
BstSFI CTRYAG 1 cut(s) 1735
BstSLI GKGCMC 2 cut(s) 417, 908
BstSNI TACGTA 1 cut(s) 1614
BstUI CGCG 3 cut(s) 408, 1587, 1589
BstV1I GCAGC 3 cut(s) 1080, 1401, 1636
BstV2I GAAGAC 1 cut(s) 32
BstZ17I GTATAC 1 cut(s) 1029
Bsu15I ATCGAT 1 cut(s) 1722
BsuI GTATCC 1 cut(s) 1538
BsuRI GGCC 3 cut(s) 257, 1219, 1675
BsuTUI ATCGAT 1 cut(s) 1722
BtgI CCRYGG 2 cut(s) 501, 1130
BtrI CACGTC 3 cut(s) 97, 412, 708
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 4 cut(s) 279, 524, 909, 1442
Cac8I GCNNGC 4 cut(s) 240, 517, 1412, 1585
CciI TCATGA 1 cut(s) 1252
CfoI GCGC 4 cut(s) 408, 430, 1410, 1589
Cfr13I GGNCC 2 cut(s) 255, 479
ClaI ATCGAT 1 cut(s) 1722
Csp6I GTAC 7 cut(s) 4, 162, 475, 656, 665, 1615, 1740
CviQI GTAC 7 cut(s) 4, 162, 475, 656, 665, 1615, 1740
DdeI CTNAG 7 cut(s) 158, 964, 1210, 1334, 1391, 1445, 1631
DpnI GATC 4 cut(s) 340, 1230, 1326, 1721
DpnII GATC 4 cut(s) 338, 1228, 1324, 1719
DraI TTTAAA 1 cut(s) 744
DraIII CACNNNGTG 1 cut(s) 1448
DriI GACNNNNNGTC 1 cut(s) 391
Eam1104I CTCTTC 2 cut(s) 501, 1168
Eam1105I GACNNNNNGTC 1 cut(s) 391
EarI CTCTTC 2 cut(s) 501, 1168
Ecl136II GAGCTC 2 cut(s) 238, 1338
Eco105I TACGTA 1 cut(s) 1614
Eco130I CCWWGG 4 cut(s) 501, 542, 1130, 1533
Eco147I AGGCCT 1 cut(s) 1675
Eco24I GRGCYC 2 cut(s) 240, 1340
Eco47I GGWCC 1 cut(s) 479
Eco53kI GAGCTC 2 cut(s) 238, 1338
Eco91I GGTNACC 1 cut(s) 1308
EcoICRI GAGCTC 2 cut(s) 238, 1338
EcoO65I GGTNACC 1 cut(s) 1308
EcoRI GAATTC 4 cut(s) 644, 946, 1065, 1565
EcoT14I CCWWGG 4 cut(s) 501, 542, 1130, 1533
EcoT38I GRGCYC 2 cut(s) 240, 1340
ErhI CCWWGG 4 cut(s) 501, 542, 1130, 1533
FalI AAGNNNNNCTT 4 cut(s) 710, 742, 738, 770
FauNDI CATATG 1 cut(s) 670
FblI GTMKAC 1 cut(s) 1028
Fnu4HI GCNGC 3 cut(s) 1094, 1415, 1650
FriOI GRGCYC 2 cut(s) 240, 1340
Fsp4HI GCNGC 3 cut(s) 1094, 1415, 1650
FspBI CTAG 4 cut(s) 543, 942, 1097, 1572
FspI TGCGCA 1 cut(s) 1409
GlaI GCGC 4 cut(s) 407, 429, 1409, 1588
GluI GCNGC 3 cut(s) 1094, 1415, 1650
HaeIII GGCC 3 cut(s) 257, 1219, 1675
HhaI GCGC 4 cut(s) 408, 430, 1410, 1589
Hin6I GCGC 4 cut(s) 406, 428, 1408, 1587
HinP1I GCGC 4 cut(s) 406, 428, 1408, 1587
HindIII AAGCTT 1 cut(s) 1346
HinfI GANTC 6 cut(s) 28, 377, 700, 923, 1237, 1724
HphI GGTGA 3 cut(s) 1046, 1145, 1302
Hpy166II GTNNAC 4 cut(s) 562, 616, 1029, 1742
Hpy188III TCNNGA 8 cut(s) 32, 289, 336, 439, 779, 1253, 1365, 1502
Hpy8I GTNNAC 4 cut(s) 562, 616, 1029, 1742
Hpy99I CGWCG 2 cut(s) 413, 712
HpyAV CCTTC 3 cut(s) 812, 1230, 1514
HpyCH4III ACNGT 4 cut(s) 195, 479, 1250, 1296
HpyCH4IV ACGT 6 cut(s) 96, 411, 707, 1056, 1267, 1613
HpyCH4V TGCA 4 cut(s) 186, 230, 919, 1181
HpyF10VI GCNNNNNNNGC 3 cut(s) 412, 965, 1414
HpyF3I CTNAG 7 cut(s) 158, 964, 1210, 1334, 1391, 1445, 1631
HpySE526I ACGT 6 cut(s) 96, 411, 707, 1056, 1267, 1613
HspAI GCGC 4 cut(s) 406, 428, 1408, 1587
Kzo9I GATC 4 cut(s) 338, 1228, 1324, 1719
LmnI GCTCC 4 cut(s) 313, 1343, 1361, 1537
Lsp1109I GCAGC 3 cut(s) 1080, 1401, 1636
LweI GCATC 2 cut(s) 784, 880
MaeI CTAG 4 cut(s) 543, 942, 1097, 1572
MaeII ACGT 6 cut(s) 96, 411, 707, 1056, 1267, 1613
MaeIII GTNAC 6 cut(s) 274, 490, 1057, 1268, 1298, 1308
MalI GATC 4 cut(s) 340, 1230, 1326, 1721
MboI GATC 4 cut(s) 338, 1228, 1324, 1719
MboII GAAGA 5 cut(s) 37, 518, 1076, 1155, 1158
MhlI GDGCHC 4 cut(s) 240, 417, 908, 1340
MlyI GAGTC 2 cut(s) 22, 1246
MmeI TCCRAC 4 cut(s) 349, 363, 869, 1643
MnlI CCTC 9 cut(s) 162, 165, 285, 508, 1014, 1053, 1315, 1346, 1400
MseI TTAA 7 cut(s) 20, 198, 354, 743, 954, 1019, 1199
MslI CAYNNNNRTG 2 cut(s) 914, 1747
MvnI CGCG 3 cut(s) 408, 1587, 1589
MwoI GCNNNNNNNGC 3 cut(s) 412, 965, 1414
NcoI CCATGG 2 cut(s) 501, 1130
NdeI CATATG 1 cut(s) 670
NdeII GATC 4 cut(s) 338, 1228, 1324, 1719
NlaIV GGNNCC 4 cut(s) 309, 1357, 1546, 1602
NmeAIII GCCGAG 1 cut(s) 437
NmuCI GTSAC 3 cut(s) 274, 1057, 1308
NsbI TGCGCA 1 cut(s) 1409
NspI RCATGY 4 cut(s) 465, 865, 1189, 1373
PagI TCATGA 1 cut(s) 1252
PceI AGGCCT 1 cut(s) 1675
PciI ACATGT 2 cut(s) 861, 1369
PfeI GAWTC 4 cut(s) 377, 700, 923, 1724
Pfl23II CGTACG 1 cut(s) 474
PkrI GCNGC 3 cut(s) 1095, 1416, 1651
PleI GAGTC 2 cut(s) 22, 1245
PpsI GAGTC 2 cut(s) 22, 1245
Ppu21I YACGTR 1 cut(s) 1614
PscI ACATGT 2 cut(s) 861, 1369
PshBI ATTAAT 1 cut(s) 954
PsiI TTATAA 1 cut(s) 1073
Psp124BI GAGCTC 2 cut(s) 240, 1340
PspEI GGTNACC 1 cut(s) 1308
PspLI CGTACG 1 cut(s) 474
PspN4I GGNNCC 4 cut(s) 309, 1357, 1546, 1602
PspPI GGNCC 2 cut(s) 255, 479
RsaI GTAC 7 cut(s) 5, 163, 476, 657, 666, 1616, 1741
RsaNI GTAC 7 cut(s) 4, 162, 475, 656, 665, 1615, 1740
RseI CAYNNNNRTG 2 cut(s) 914, 1747
SacI GAGCTC 2 cut(s) 240, 1340
SaqAI TTAA 7 cut(s) 20, 198, 354, 743, 954, 1019, 1199
SatI GCNGC 3 cut(s) 1094, 1415, 1650
Sau3AI GATC 4 cut(s) 338, 1228, 1324, 1719
Sau96I GGNCC 2 cut(s) 255, 479
SchI GAGTC 2 cut(s) 22, 1246
SduI GDGCHC 4 cut(s) 240, 417, 908, 1340
SfaNI GCATC 2 cut(s) 784, 880
SfcI CTRYAG 1 cut(s) 1735
SinI GGWCC 1 cut(s) 479
SmiMI CAYNNNNRTG 2 cut(s) 914, 1747
SmlI CTYRAG 2 cut(s) 289, 1088
SmoI CTYRAG 2 cut(s) 289, 1088
SnaBI TACGTA 1 cut(s) 1614
SpeI ACTAGT 1 cut(s) 1571
SseBI AGGCCT 1 cut(s) 1675
SsiI CCGC 2 cut(s) 263, 1313
SspI AATATT 1 cut(s) 38
SspMI CTAG 4 cut(s) 543, 942, 1097, 1572
SstI GAGCTC 2 cut(s) 240, 1340
StuI AGGCCT 1 cut(s) 1675
StyI CCWWGG 4 cut(s) 501, 542, 1130, 1533
TaaI ACNGT 4 cut(s) 195, 479, 1250, 1296
TaiI ACGT 6 cut(s) 99, 414, 710, 1059, 1270, 1616
TaqI TCGA 6 cut(s) 206, 234, 710, 1563, 1688, 1722
TatI WGTACW 2 cut(s) 3, 1739
TfiI GAWTC 4 cut(s) 377, 700, 923, 1724
Tru1I TTAA 7 cut(s) 20, 198, 354, 743, 954, 1019, 1199
Tru9I TTAA 7 cut(s) 20, 198, 354, 743, 954, 1019, 1199
TscAI CASTG 4 cut(s) 279, 531, 916, 1449
TseFI GTSAC 3 cut(s) 274, 1057, 1308
TseI GCWGC 3 cut(s) 1093, 1414, 1649
Tsp45I GTSAC 3 cut(s) 274, 1057, 1308
TspDTI ATGAA 4 cut(s) 126, 911, 1249, 1627
TspGWI ACGGA 2 cut(s) 610, 1732
TspRI CASTG 4 cut(s) 279, 531, 916, 1449
VpaK11BI GGWCC 1 cut(s) 479
VspI ATTAAT 1 cut(s) 954
XapI RAATTY 8 cut(s) 601, 644, 681, 946, 1065, 1378, 1455, 1565
XceI RCATGY 4 cut(s) 465, 865, 1189, 1373
XmaJI CCTAGG 1 cut(s) 542
XmiI GTMKAC 1 cut(s) 1028
XspI CTAG 4 cut(s) 543, 942, 1097, 1572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.