pycom01g14630

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
N/A
Physical Location & Seq
Forward (+)
14735704 .. 14738285
2582 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 831 bp
ATGTTGTTTCGGTTATTTATTTATTTTCTTGAGCTTTTTCAGTCACGTCTCCATTATTCTTCTTCTATTTTACACTCCATAATTTTTATCTCAACCTTATCAAAAGTCCAAATATATGTGGGAGTCCAAGTTGACCACATTATCACTCCAAGGCAGGCCTATATATATGCATCACATGTATACGTTGGTTTCTATATACACAATATCAACTCCCCTATACTCTCCACTCTAAATCCTAATTTGTTAAATTCCATCAGAGTTTTGCGTATTTCTCTCTCACTGATCATACGATTAGCAGTCTTCCAGAAAAACCTCGGCTTCGTCCTGATATCAAGCACTCTACGCATCATGGCCTCTGACATGGATTTTGTCAAATATGAAGAGGAGTACATCTTAAATCCTAGAGGCATGAAGCTTTTCACATGCAGATGGCTCCCGAAGAACACCAAACCCAAAGCCCTAATCTGCATCTGCCATGGGTACGCCATGGAATGCAGCATCACCATGAACAGCACTGCAATCAGGCTAGCCAAAGCAGGATTCGCCATATATGGAATAGACTATGAAGGCCATGGAAAATCAGCAGGTCTACAAGGTTACGTGAAGAGCTTTGATGATGTGGTGGATGACTGTACCAACCACTTCACAAACATATGTGAGAGCAAAGAGAACAACGGAACGATGAGGTATCTAATGGGGGAGTCGATGGGAGGAGCTGTGGCTCTGCTGGTGCACAGGAAAAAGCCAGAGTTTTGGGATGGTGCGGTTTTGGTTGCACCCATGTGTAAGTTTGAATGTTTCACCATTTTAGGGTGCTCACTTTATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

277

Amino Acids

31.32

Weight (kDa)

8.78

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 150 - 264 1.6e-33 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 154 - 260 9.4e-07 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 575
AccI GTMKAC 2 cut(s) 180, 589
AciI CCGC 1 cut(s) 764
AcsI RAATTY 1 cut(s) 247
AfaI GTAC 3 cut(s) 389, 482, 634
AfiI CCNNNNNNNGG 1 cut(s) 810
AflIII ACRYGT 1 cut(s) 175
AgsI TTSAA 1 cut(s) 794
AjiI CACGTC 1 cut(s) 47
AjuI GAANNNNNNNTTGG 2 cut(s) 524, 556
AleI CACNNNNGTG 1 cut(s) 781
AluBI AGCT 4 cut(s) 34, 415, 609, 716
AluI AGCT 4 cut(s) 34, 415, 609, 716
Alw21I GWGCWC 2 cut(s) 735, 818
Alw26I GTCTC 1 cut(s) 53
Alw44I GTGCAC 1 cut(s) 731
AoxI GGCC 3 cut(s) 156, 351, 568
ApaLI GTGCAC 1 cut(s) 731
ApeKI GCWGC 1 cut(s) 495
ApoI RAATTY 1 cut(s) 247
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
Asp700I GAANNNNTTC 1 cut(s) 416
AsuHPI GGTGA 2 cut(s) 493, 793
AsuNHI GCTAGC 1 cut(s) 526
BaeGI GKGCMC 1 cut(s) 735
BbsI GAAGAC 1 cut(s) 292
Bbv12I GWGCWC 2 cut(s) 735, 818
BbvI GCAGC 1 cut(s) 507
BccI CCATC 4 cut(s) 260, 423, 700, 752
BclI TGATCA 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 2 cut(s) 402, 527
BfuAI ACCTGC 1 cut(s) 575
BisI GCNGC 1 cut(s) 496
BlsI GCNGC 1 cut(s) 497
BmgBI CACGTC 1 cut(s) 47
BmiI GGNNCC 1 cut(s) 434
BmsI GCATC 4 cut(s) 179, 354, 477, 507
BmtI GCTAGC 1 cut(s) 530
BpiI GAAGAC 1 cut(s) 292
BpuEI CTTGAG 1 cut(s) 50
BsaAI YACGTR 1 cut(s) 601
BsaJI CCNNGG 5 cut(s) 149, 313, 475, 486, 571
BsaXI ACNNNNNCTCC 4 cut(s) 114, 144, 702, 732
Bsc4I CCNNNNNNNGG 1 cut(s) 810
BseDI CCNNGG 5 cut(s) 149, 313, 475, 486, 571
BseGI GGATG 2 cut(s) 631, 763
BseLI CCNNNNNNNGG 1 cut(s) 810
BseRI GAGGAG 2 cut(s) 398, 726
BseSI GKGCMC 1 cut(s) 735
BseXI GCAGC 1 cut(s) 507
BshFI GGCC 3 cut(s) 158, 353, 570
BsiHKAI GWGCWC 2 cut(s) 735, 818
BslI CCNNNNNNNGG 1 cut(s) 810
BsmAI GTCTC 1 cut(s) 53
BsmBI CGTCTC 1 cut(s) 53
BsmI GAATGC 1 cut(s) 497
BsnI GGCC 3 cut(s) 158, 353, 570
Bsp1286I GDGCHC 2 cut(s) 735, 818
Bsp143I GATC 1 cut(s) 282
Bsp19I CCATGG 3 cut(s) 475, 486, 571
BspACI CCGC 1 cut(s) 764
BspANI GGCC 3 cut(s) 158, 353, 570
BspLI GGNNCC 1 cut(s) 434
BspMI ACCTGC 1 cut(s) 575
BspOI GCTAGC 1 cut(s) 530
BspQI GCTCTTC 1 cut(s) 599
BssECI CCNNGG 5 cut(s) 149, 313, 475, 486, 571
BssMI GATC 1 cut(s) 282
BssNAI GTATAC 1 cut(s) 181
BssT1I CCWWGG 4 cut(s) 149, 475, 486, 571
Bst1107I GTATAC 1 cut(s) 181
Bst4CI ACNGT 1 cut(s) 632
Bst6I CTCTTC 2 cut(s) 375, 599
BstBAI YACGTR 1 cut(s) 601
BstC8I GCNNGC 2 cut(s) 156, 528
BstDSI CCRYGG 3 cut(s) 475, 486, 571
BstF5I GGATG 2 cut(s) 631, 763
BstKTI GATC 1 cut(s) 285
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 1 cut(s) 282
BstMWI GCNNNNNNNGC 2 cut(s) 342, 542
BstNSI RCATGY 2 cut(s) 179, 426
BstSLI GKGCMC 1 cut(s) 735
BstV1I GCAGC 1 cut(s) 507
BstV2I GAAGAC 1 cut(s) 292
BstXI CCANNNNNNTGG 1 cut(s) 753
BstZ17I GTATAC 1 cut(s) 181
BsuRI GGCC 3 cut(s) 158, 353, 570
BtgI CCRYGG 3 cut(s) 475, 486, 571
BtrI CACGTC 1 cut(s) 47
BtsCI GGATG 2 cut(s) 631, 763
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 2 cut(s) 278, 513
BveI ACCTGC 1 cut(s) 575
Cac8I GCNNGC 2 cut(s) 156, 528
Csp6I GTAC 3 cut(s) 388, 481, 633
CviQI GTAC 3 cut(s) 388, 481, 633
DpnI GATC 1 cut(s) 284
DpnII GATC 1 cut(s) 282
Eam1104I CTCTTC 2 cut(s) 375, 599
EarI CTCTTC 2 cut(s) 375, 599
Eco130I CCWWGG 4 cut(s) 149, 475, 486, 571
Eco147I AGGCCT 1 cut(s) 158
Eco32I GATATC 1 cut(s) 330
EcoRV GATATC 1 cut(s) 330
EcoT14I CCWWGG 4 cut(s) 149, 475, 486, 571
EcoT22I ATGCAT 1 cut(s) 172
ErhI CCWWGG 4 cut(s) 149, 475, 486, 571
Esp3I CGTCTC 1 cut(s) 53
FauNDI CATATG 1 cut(s) 653
FbaI TGATCA 1 cut(s) 282
FblI GTMKAC 2 cut(s) 180, 589
Fnu4HI GCNGC 1 cut(s) 496
FokI GGATG 2 cut(s) 638, 770
Fsp4HI GCNGC 1 cut(s) 496
FspBI CTAG 2 cut(s) 402, 527
GluI GCNGC 1 cut(s) 496
HaeIII GGCC 3 cut(s) 158, 353, 570
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HindIII AAGCTT 1 cut(s) 413
HinfI GANTC 3 cut(s) 123, 540, 701
HphI GGTGA 2 cut(s) 493, 793
Hpy166II GTNNAC 4 cut(s) 133, 181, 590, 733
Hpy188I TCNGA 2 cut(s) 257, 358
Hpy188III TCNNGA 4 cut(s) 29, 304, 325, 436
Hpy8I GTNNAC 4 cut(s) 133, 181, 590, 733
HpyAV CCTTC 1 cut(s) 560
HpyCH4III ACNGT 1 cut(s) 632
HpyCH4IV ACGT 3 cut(s) 46, 183, 600
HpyCH4V TGCA 7 cut(s) 170, 426, 468, 495, 518, 733, 776
HpyF10VI GCNNNNNNNGC 2 cut(s) 342, 542
HpySE526I ACGT 3 cut(s) 46, 183, 600
Ksp22I TGATCA 1 cut(s) 282
Kzo9I GATC 1 cut(s) 282
LguI GCTCTTC 1 cut(s) 599
LmnI GCTCC 2 cut(s) 438, 713
LpnPI CCDG 9 cut(s) 140, 317, 338, 508, 522, 570, 713, 721, 759
Lsp1109I GCAGC 1 cut(s) 507
LweI GCATC 4 cut(s) 179, 354, 477, 507
MaeI CTAG 2 cut(s) 402, 527
MaeII ACGT 3 cut(s) 46, 183, 600
MaeIII GTNAC 2 cut(s) 42, 596
MalI GATC 1 cut(s) 284
MboI GATC 1 cut(s) 282
MboII GAAGA 6 cut(s) 51, 54, 292, 392, 451, 616
MhlI GDGCHC 2 cut(s) 735, 818
MluCI AATT 3 cut(s) 81, 238, 247
MlyI GAGTC 2 cut(s) 132, 710
MnlI CCTC 6 cut(s) 323, 364, 376, 398, 678, 704
Mph1103I ATGCAT 1 cut(s) 172
MroXI GAANNNNTTC 1 cut(s) 416
MseI TTAA 2 cut(s) 245, 395
MslI CAYNNNNRTG 3 cut(s) 427, 503, 781
Mva1269I GAATGC 1 cut(s) 497
MwoI GCNNNNNNNGC 2 cut(s) 342, 542
NcoI CCATGG 3 cut(s) 475, 486, 571
NdeI CATATG 1 cut(s) 653
NdeII GATC 1 cut(s) 282
NheI GCTAGC 1 cut(s) 526
NlaIV GGNNCC 1 cut(s) 434
NmeAIII GCCGAG 1 cut(s) 294
NmuCI GTSAC 1 cut(s) 42
NsiI ATGCAT 1 cut(s) 172
NspI RCATGY 2 cut(s) 179, 426
OliI CACNNNNGTG 1 cut(s) 781
PceI AGGCCT 1 cut(s) 158
PciI ACATGT 1 cut(s) 175
PciSI GCTCTTC 1 cut(s) 599
PctI GAATGC 1 cut(s) 497
PdmI GAANNNNTTC 1 cut(s) 416
PfeI GAWTC 1 cut(s) 540
PkrI GCNGC 1 cut(s) 497
PleI GAGTC 2 cut(s) 131, 709
PpsI GAGTC 2 cut(s) 131, 709
Ppu21I YACGTR 1 cut(s) 601
PscI ACATGT 1 cut(s) 175
PspN4I GGNNCC 1 cut(s) 434
RsaI GTAC 3 cut(s) 389, 482, 634
RsaNI GTAC 3 cut(s) 388, 481, 633
RseI CAYNNNNRTG 3 cut(s) 427, 503, 781
SapI GCTCTTC 1 cut(s) 599
SaqAI TTAA 2 cut(s) 245, 395
SatI GCNGC 1 cut(s) 496
Sau3AI GATC 1 cut(s) 282
SchI GAGTC 2 cut(s) 132, 710
SduI GDGCHC 2 cut(s) 735, 818
SfaNI GCATC 4 cut(s) 179, 354, 477, 507
SmiMI CAYNNNNRTG 3 cut(s) 427, 503, 781
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 3 cut(s) 81, 238, 247
SseBI AGGCCT 1 cut(s) 158
SsiI CCGC 1 cut(s) 764
SspMI CTAG 2 cut(s) 402, 527
StuI AGGCCT 1 cut(s) 158
StyI CCWWGG 4 cut(s) 149, 475, 486, 571
TaaI ACNGT 1 cut(s) 632
TaiI ACGT 3 cut(s) 49, 186, 603
TaqI TCGA 1 cut(s) 704
TasI AATT 3 cut(s) 81, 238, 247
TatI WGTACW 1 cut(s) 387
TfiI GAWTC 1 cut(s) 540
Tru1I TTAA 2 cut(s) 245, 395
Tru9I TTAA 2 cut(s) 245, 395
TscAI CASTG 2 cut(s) 285, 520
TseFI GTSAC 1 cut(s) 42
TseI GCWGC 1 cut(s) 495
Tsp45I GTSAC 1 cut(s) 42
TspDTI ATGAA 4 cut(s) 393, 425, 521, 579
TspGWI ACGGA 1 cut(s) 690
TspRI CASTG 2 cut(s) 285, 520
VneI GTGCAC 1 cut(s) 731
XapI RAATTY 1 cut(s) 247
XceI RCATGY 2 cut(s) 179, 426
XmiI GTMKAC 2 cut(s) 180, 589
XmnI GAANNNNTTC 1 cut(s) 416
XspI CTAG 2 cut(s) 402, 527
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.