pycom01g14920

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
N/A
Physical Location & Seq
Reverse (-)
14966367 .. 14968168
1802 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 624 bp
ATGCAAAACCACCTCTTACTTGACGTAAATGACATGCCATCTCTCATTCAACGCTTCCTAAGGCGGAAAGCTCCCACATATTCCTGCCGCGACCCGCAGCCCTCTTCTTCCACCGCTGAGACTCTATCCATCTCTCTCTCTCTCTCTCTCCGCCGATATTTCTCGGGAAAATGGAACCGAGAGTTGATAAACCGTGATGGGAAAATAGCAAATGCAAGATCCGGGATGGCTATGCATGACGAATGCAAGCTAAAGTTTTTAGAATTGAAGGCAAAAAGAACTTACCGCTTCATAGTTTACAAGATCGAGGAGAAGCAAAATGAGGTTATAGTGGAAAAGCTTGGCGAGCCAACTGACAGCTATGAGGATTTCTCTGCAAGCCTCCCTCCTAACGAGTGCCGATACGCTGTTTATGACTTTGATTACGTGACGGAGGAGAACTGCCAGAAGAGCCGGATTATTTTCGTTGCTTGGTCCCCTGACACGGCGAAGGTGAGAAGCAAGATGATTTACGCGAGTTCGAAGGACACGTTTAAGAGAGAGTTGGACGGAATTCAGATGGAGTTGCAAGCTACTGATCCAACCGAAGTGGGTCTCGATGTTATTAGAAGCCGTGCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

208

Amino Acids

23.97

Weight (kDa)

8.27

Isoelectric Point (pI)

58.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 75 - 206 2.4e-41 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 90, 515
AciI CCGC 6 cut(s) 64, 88, 95, 114, 151, 286
AclWI GGATC 2 cut(s) 213, 572
AcsI RAATTY 1 cut(s) 552
AfiI CCNNNNNNNGG 1 cut(s) 484
AflIII ACRYGT 1 cut(s) 528
AgsI TTSAA 2 cut(s) 50, 268
AluBI AGCT 5 cut(s) 71, 250, 340, 360, 572
AluI AGCT 5 cut(s) 71, 250, 340, 360, 572
Alw26I GTCTC 2 cut(s) 113, 599
AlwI GGATC 2 cut(s) 213, 572
Ama87I CYCGRG 1 cut(s) 163
ApeKI GCWGC 1 cut(s) 97
ApoI RAATTY 1 cut(s) 552
AspS9I GGNCC 1 cut(s) 474
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 1 cut(s) 505
AsuII TTCGAA 1 cut(s) 521
AvaI CYCGRG 1 cut(s) 163
AvaII GGWCC 1 cut(s) 474
AxyI CCTNAGG 1 cut(s) 59
BbvI GCAGC 1 cut(s) 109
BccI CCATC 5 cut(s) 46, 137, 191, 220, 553
BceAI ACGGC 2 cut(s) 501, 597
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 2 cut(s) 113, 599
BisI GCNGC 2 cut(s) 88, 98
BlsI GCNGC 2 cut(s) 89, 99
Bme1390I CCNGG 1 cut(s) 223
Bme18I GGWCC 1 cut(s) 474
BmeT110I CYCGRG 1 cut(s) 163
BmgT120I GGNCC 1 cut(s) 474
BmiI GGNNCC 2 cut(s) 176, 476
BmrFI CCNGG 1 cut(s) 223
BplI GAGNNNNNCTC 2 cut(s) 356, 388
Bpu14I TTCGAA 1 cut(s) 521
BpuMI CCSGG 1 cut(s) 223
BsaAI YACGTR 1 cut(s) 427
BsaI GGTCTC 1 cut(s) 599
Bsc4I CCNNNNNNNGG 1 cut(s) 484
Bse21I CCTNAGG 1 cut(s) 59
BseGI GGATG 1 cut(s) 231
BseLI CCNNNNNNNGG 1 cut(s) 484
BseMII CTCAG 1 cut(s) 108
BseRI GAGGAG 2 cut(s) 323, 449
BseXI GCAGC 1 cut(s) 109
Bsh1236I CGCG 2 cut(s) 90, 515
BsiHKCI CYCGRG 1 cut(s) 163
BsiSI CCGG 2 cut(s) 222, 454
BslFI GGGAC 1 cut(s) 460
BslI CCNNNNNNNGG 1 cut(s) 484
BsmAI GTCTC 2 cut(s) 113, 599
BsmFI GGGAC 1 cut(s) 460
BsmI GAATGC 1 cut(s) 248
Bso31I GGTCTC 1 cut(s) 599
BsoBI CYCGRG 1 cut(s) 163
Bsp119I TTCGAA 1 cut(s) 521
Bsp143I GATC 3 cut(s) 218, 303, 577
BspACI CCGC 6 cut(s) 64, 88, 95, 114, 151, 286
BspCNI CTCAG 1 cut(s) 109
BspFNI CGCG 2 cut(s) 90, 515
BspLI GGNNCC 2 cut(s) 176, 476
BspPI GGATC 2 cut(s) 213, 572
BspQI GCTCTTC 1 cut(s) 443
BspT104I TTCGAA 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 599
BssMI GATC 3 cut(s) 218, 303, 577
Bst4CI ACNGT 1 cut(s) 194
Bst6I CTCTTC 2 cut(s) 109, 443
BstBAI YACGTR 1 cut(s) 427
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 4 cut(s) 248, 347, 379, 570
BstDEI CTNAG 2 cut(s) 59, 117
BstF5I GGATG 1 cut(s) 231
BstFNI CGCG 2 cut(s) 90, 515
BstKTI GATC 3 cut(s) 221, 306, 580
BstMAI GTCTC 2 cut(s) 113, 599
BstMBI GATC 3 cut(s) 218, 303, 577
BstMWI GCNNNNNNNGC 2 cut(s) 346, 450
BstNSI RCATGY 1 cut(s) 37
BstSCI CCNGG 1 cut(s) 221
BstUI CGCG 2 cut(s) 90, 515
BstV1I GCAGC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 218
BstYI RGATCY 1 cut(s) 218
Bsu36I CCTNAGG 1 cut(s) 59
BtsCI GGATG 1 cut(s) 231
Cac8I GCNNGC 4 cut(s) 248, 347, 379, 570
Cfr13I GGNCC 1 cut(s) 474
CspCI CAANNNNNGTGG 2 cut(s) 570, 605
CviAII CATG 2 cut(s) 34, 236
DdeI CTNAG 2 cut(s) 59, 117
DpnI GATC 3 cut(s) 220, 305, 579
DpnII GATC 3 cut(s) 218, 303, 577
Eam1104I CTCTTC 2 cut(s) 109, 443
EarI CTCTTC 2 cut(s) 109, 443
EciI GGCGGA 2 cut(s) 79, 140
Eco31I GGTCTC 1 cut(s) 599
Eco47I GGWCC 1 cut(s) 474
Eco81I CCTNAGG 1 cut(s) 59
Eco88I CYCGRG 1 cut(s) 163
EcoRI GAATTC 1 cut(s) 552
EcoT22I ATGCAT 1 cut(s) 237
FaeI CATG 2 cut(s) 37, 239
FaiI YATR 8 cut(s) 35, 79, 233, 237, 293, 329, 363, 414
FaqI GGGAC 1 cut(s) 460
FatI CATG 2 cut(s) 33, 235
FauI CCCGC 1 cut(s) 102
Fnu4HI GCNGC 2 cut(s) 88, 98
FokI GGATG 1 cut(s) 238
Fsp4HI GCNGC 2 cut(s) 88, 98
GluI GCNGC 2 cut(s) 88, 98
HapII CCGG 2 cut(s) 222, 454
Hin1II CATG 2 cut(s) 37, 239
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 1 cut(s) 121
HpaII CCGG 2 cut(s) 222, 454
HphI GGTGA 1 cut(s) 505
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 1 cut(s) 558
Hpy188III TCNNGA 2 cut(s) 165, 596
Hpy8I GTNNAC 1 cut(s) 298
HpyAV CCTTC 3 cut(s) 262, 484, 517
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4IV ACGT 3 cut(s) 24, 426, 530
HpyCH4V TGCA 6 cut(s) 4, 215, 235, 246, 377, 568
HpyF10VI GCNNNNNNNGC 2 cut(s) 346, 450
HpyF3I CTNAG 2 cut(s) 59, 117
HpySE526I ACGT 3 cut(s) 24, 426, 530
Hsp92II CATG 2 cut(s) 37, 239
Kzo9I GATC 3 cut(s) 218, 303, 577
LguI GCTCTTC 1 cut(s) 443
LmnI GCTCC 1 cut(s) 76
LpnPI CCDG 5 cut(s) 97, 235, 458, 467, 492
Lsp1109I GCAGC 1 cut(s) 109
MaeII ACGT 3 cut(s) 24, 426, 530
MaeIII GTNAC 1 cut(s) 427
MalI GATC 3 cut(s) 220, 305, 579
MboI GATC 3 cut(s) 218, 303, 577
MboII GAAGA 3 cut(s) 96, 99, 460
MflI RGATCY 1 cut(s) 218
MluCI AATT 3 cut(s) 263, 552, 619
MlyI GAGTC 1 cut(s) 115
MmeI TCCRAC 2 cut(s) 525, 605
MnlI CCTC 8 cut(s) 23, 112, 301, 316, 358, 392, 396, 427
Mph1103I ATGCAT 1 cut(s) 237
MseI TTAA 1 cut(s) 534
MspA1I CMGCKG 1 cut(s) 116
MspI CCGG 2 cut(s) 222, 454
MspR9I CCNGG 1 cut(s) 223
Mva1269I GAATGC 1 cut(s) 248
MvnI CGCG 2 cut(s) 90, 515
MwoI GCNNNNNNNGC 2 cut(s) 346, 450
NciI CCSGG 1 cut(s) 223
NdeII GATC 3 cut(s) 218, 303, 577
NlaIII CATG 2 cut(s) 37, 239
NlaIV GGNNCC 2 cut(s) 176, 476
NmuCI GTSAC 1 cut(s) 427
NsiI ATGCAT 1 cut(s) 237
NspI RCATGY 1 cut(s) 37
NspV TTCGAA 1 cut(s) 521
PciSI GCTCTTC 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 527
PctI GAATGC 1 cut(s) 248
PfoI TCCNGGA 1 cut(s) 221
PkrI GCNGC 2 cut(s) 89, 99
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
Ppu21I YACGTR 1 cut(s) 427
PspN4I GGNNCC 2 cut(s) 176, 476
PspPI GGNCC 1 cut(s) 474
PsuI RGATCY 1 cut(s) 218
SapI GCTCTTC 1 cut(s) 443
SaqAI TTAA 1 cut(s) 534
SatI GCNGC 2 cut(s) 88, 98
Sau3AI GATC 3 cut(s) 218, 303, 577
Sau96I GGNCC 1 cut(s) 474
SchI GAGTC 1 cut(s) 115
ScrFI CCNGG 1 cut(s) 223
SfuI TTCGAA 1 cut(s) 521
SinI GGWCC 1 cut(s) 474
Sse9I AATT 3 cut(s) 263, 552, 619
SsiI CCGC 6 cut(s) 64, 88, 95, 114, 151, 286
StyD4I CCNGG 1 cut(s) 221
TaaI ACNGT 1 cut(s) 194
TaiI ACGT 3 cut(s) 27, 429, 533
TaqI TCGA 3 cut(s) 306, 521, 597
TasI AATT 3 cut(s) 263, 552, 619
TauI GCSGC 1 cut(s) 90
Tru1I TTAA 1 cut(s) 534
Tru9I TTAA 1 cut(s) 534
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 1 cut(s) 97
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 1 cut(s) 280
TspGWI ACGGA 2 cut(s) 446, 564
VpaK11BI GGWCC 1 cut(s) 474
XapI RAATTY 1 cut(s) 552
XceI RCATGY 1 cut(s) 37
Zsp2I ATGCAT 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.