pycom01g15110

ankyrin repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0028606
Physical Location & Seq
Reverse (-)
15096757 .. 15098076
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1320 bp
ATGGCATCGTGTGTTCAAGCTTTTCATGGCCGAACGACAAATACTATTGCATGTGCAGTCACTGAAATGGCTCAAAGGTTACGTGATTTTGTCCTGGAATGCGATGGTCATGTGTTATTAGAGGGAGAACCTATTGCTAAGGAAATTCATGAGTTTATTGAATGGATAGGGCCAGGCTTCAATTTTAGTGGTCATCAGGGTAATAAGGATAGAGCTAATTACAACTTACAGGCTGATGAGATCCAACTTCAGTTATCGGCATTGAAAACCTTTCTAGATCTTGCTGGTAACCAGCTTACATCAAAAGACTACTCAATGGCCTTCTATGCTGCTTGCCTTCCCCTATATTTATCCTCTAGTTCTTTCGATCTGGGTTGCGCGTCTGGGAATTGGGAAACTACTGCAATACCAGGTTTGTTTGGTATGCTGGTGAAAGGGGGTGTGGAGAGTGGCATTTTCAATTATTCTTTGGTTCAAGCGTCTAGTTTTGGGAATACAGAGATTGTGCACATCATATTGAAGGCTGCCCGAATTAACAACCTGAATGTCGATGTTGACCGGGCATTTAATTTTGCTTGCCTGTACTCTAGGTTTGGTACTATGGAATGTCTTGTGGAAGAAGGTAATATCATGGATTTCAGGACCCCTCTGGTGGGAGCTGCTAAGAGCGGCTGTAAGCCAGTGGTTGAGTGGTTTGTAAAAATGGGTTGCCGGGACACAGAACTGTGCATTGCTCTGGTAAGTGCAACTTCTAGCAACCAAGCTGATGTTGCAGTCTATCTCCTTCATCAACTTCCTGATGATCTCCTTGCACAACGAAGCTTTAATATTCTCAAGGCTGCTGGTAGAGTAGGCGGTGTTAATTCTCTTAAAGGTGTTGCATTTCTTCTTGGTTCTGATTTCTTAGGTGACCCTGCAACTACTTATACTGTTGCGGACACATTTGCTCGGTCTTGTGATGATGATGATGCTAATCCTGAGCTAAGGGCGTTTCTTCAGGAGCATTGGTCAGAAAGAGCTTTCTCAGACGGAATACGGCAAGGACAAGAACATTACATGAACATTGTGAGGGTTTTAAAGTGGGGTGAATCTCCCATCTGCTTGGCTGTTCTACCCCCCGAATTGAGGGTAGCCATTGCTTACCTGCGACTATATAGGGAGTGTGTCACTGCAGCAGGCAGCCGTTTGGTGTTGCAAAGGTTGAGAGGGCAGCTTGTTGAAGCTGTGAGAAGGCTTGGTGGTGGAGTGTTAGATGGGGTGACCCAGTGCAGGGAGCTCTTAGCTGTTTTGGAGAAGCGTCTTCCTCAATTCACTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

440

Amino Acids

48.18

Weight (kDa)

5.39

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028606)

Species Orthologous Gene IDs
malus_domestica MD01G1124800.v1.1
pyrus_communis pycom01g15110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1152
AccBSI CCGCTC 1 cut(s) 669
AccII CGCG 1 cut(s) 380
AciI CCGC 3 cut(s) 669, 855, 935
AclWI GGATC 1 cut(s) 235
AcoI YGGCCR 1 cut(s) 28
AcsI RAATTY 1 cut(s) 144
AcuI CTGAAG 2 cut(s) 233, 980
AfaI GTAC 2 cut(s) 584, 598
AfiI CCNNNNNNNGG 5 cut(s) 652, 653, 1125, 1269, 1270
AgsI TTSAA 8 cut(s) 17, 161, 181, 265, 460, 476, 520, 1220
AjnI CCWGG 3 cut(s) 93, 172, 409
Alw21I GWGCWC 2 cut(s) 510, 1278
Alw44I GTGCAC 1 cut(s) 506
AlwI GGATC 1 cut(s) 235
AlwNI CAGNNNCTG 1 cut(s) 62
AoxI GGCC 3 cut(s) 28, 170, 318
ApaLI GTGCAC 1 cut(s) 506
ApeKI GCWGC 7 cut(s) 329, 524, 659, 839, 1172, 1179, 1210
ApoI RAATTY 1 cut(s) 144
AspLEI GCGC 1 cut(s) 380
AspS9I GGNCC 2 cut(s) 170, 642
AsuC2I CCSGG 2 cut(s) 560, 713
AsuHPI GGTGA 4 cut(s) 442, 920, 1097, 1270
AvaII GGWCC 1 cut(s) 642
BaeGI GKGCMC 1 cut(s) 510
BanII GRGCYC 1 cut(s) 1278
BbsI GAAGAC 1 cut(s) 1292
Bbv12I GWGCWC 2 cut(s) 510, 1278
BbvI GCAGC 7 cut(s) 316, 511, 646, 826, 1184, 1191, 1222
BccI CCATC 3 cut(s) 98, 1103, 1247
BceAI ACGGC 2 cut(s) 1052, 1167
BciT130I CCWGG 3 cut(s) 95, 174, 411
BcnI CCSGG 2 cut(s) 560, 713
BfaI CTAG 5 cut(s) 275, 357, 483, 588, 753
BfmI CTRYAG 1 cut(s) 1170
BfuAI ACCTGC 1 cut(s) 1152
BglII AGATCT 1 cut(s) 277
BisI GCNGC 8 cut(s) 330, 525, 660, 670, 840, 1173, 1180, 1211
BlsI GCNGC 8 cut(s) 331, 526, 661, 671, 841, 1174, 1181, 1212
Bme1390I CCNGG 5 cut(s) 95, 174, 411, 560, 713
Bme18I GGWCC 1 cut(s) 642
BmgT120I GGNCC 2 cut(s) 170, 642
BmiI GGNNCC 1 cut(s) 644
BmrFI CCNGG 5 cut(s) 95, 174, 411, 560, 713
BmrI ACTGGG 1 cut(s) 1258
BmsI GCATC 2 cut(s) 14, 958
BmuI ACTGGG 1 cut(s) 1258
BpiI GAAGAC 1 cut(s) 1292
Bpu10I CCTNAGC 3 cut(s) 138, 978, 983
BpuEI CTTGAG 1 cut(s) 818
BpuMI CCSGG 2 cut(s) 560, 713
BsaAI YACGTR 1 cut(s) 83
BsaBI GATNNNNATC 1 cut(s) 972
BsaXI ACNNNNNCTCC 2 cut(s) 992, 1022
Bsc4I CCNNNNNNNGG 5 cut(s) 652, 653, 1125, 1269, 1270
Bse1I ACTGG 2 cut(s) 680, 1264
Bse3DI GCAATG 2 cut(s) 729, 1134
Bse8I GATNNNNATC 1 cut(s) 972
BseBI CCWGG 3 cut(s) 95, 174, 411
BseJI GATNNNNATC 1 cut(s) 972
BseLI CCNNNNNNNGG 5 cut(s) 652, 653, 1125, 1269, 1270
BseMI GCAATG 2 cut(s) 729, 1134
BseMII CTCAG 2 cut(s) 969, 1038
BseNI ACTGG 2 cut(s) 680, 1264
BseSI GKGCMC 1 cut(s) 510
BseXI GCAGC 7 cut(s) 316, 511, 646, 826, 1184, 1191, 1222
BsgI GTGCAG 2 cut(s) 75, 1288
Bsh1236I CGCG 1 cut(s) 380
BshFI GGCC 3 cut(s) 30, 172, 320
BsiHKAI GWGCWC 2 cut(s) 510, 1278
BsiSI CCGG 2 cut(s) 559, 712
BslFI GGGAC 1 cut(s) 728
BslI CCNNNNNNNGG 5 cut(s) 652, 653, 1125, 1269, 1270
BsmFI GGGAC 1 cut(s) 728
BsmI GAATGC 1 cut(s) 104
BsnI GGCC 3 cut(s) 30, 172, 320
Bsp1286I GDGCHC 2 cut(s) 510, 1278
Bsp143I GATC 4 cut(s) 240, 277, 367, 802
BspACI CCGC 3 cut(s) 669, 855, 935
BspANI GGCC 3 cut(s) 30, 172, 320
BspCNI CTCAG 2 cut(s) 970, 1037
BspFNI CGCG 1 cut(s) 380
BspHI TCATGA 1 cut(s) 148
BspLI GGNNCC 1 cut(s) 644
BspMAI CTGCAG 1 cut(s) 1174
BspMI ACCTGC 1 cut(s) 1152
BspPI GGATC 1 cut(s) 235
BsrBI CCGCTC 1 cut(s) 669
BsrDI GCAATG 2 cut(s) 729, 1134
BsrI ACTGG 2 cut(s) 680, 1264
BssMI GATC 4 cut(s) 240, 277, 367, 802
Bst2UI CCWGG 3 cut(s) 95, 174, 411
Bst4CI ACNGT 2 cut(s) 726, 931
BstBAI YACGTR 1 cut(s) 83
BstC8I GCNNGC 3 cut(s) 334, 577, 1177
BstDEI CTNAG 7 cut(s) 138, 663, 904, 978, 983, 1024, 1279
BstEII GGTNACC 3 cut(s) 287, 908, 1258
BstFNI CGCG 1 cut(s) 380
BstHHI GCGC 1 cut(s) 380
BstKTI GATC 4 cut(s) 243, 280, 370, 805
BstMBI GATC 4 cut(s) 240, 277, 367, 802
BstMWI GCNNNNNNNGC 2 cut(s) 326, 770
BstNI CCWGG 3 cut(s) 95, 174, 411
BstNSI RCATGY 1 cut(s) 54
BstPI GGTNACC 3 cut(s) 287, 908, 1258
BstSCI CCNGG 5 cut(s) 93, 172, 409, 558, 711
BstSFI CTRYAG 1 cut(s) 1170
BstSLI GKGCMC 1 cut(s) 510
BstUI CGCG 1 cut(s) 380
BstV1I GCAGC 7 cut(s) 316, 511, 646, 826, 1184, 1191, 1222
BstV2I GAAGAC 1 cut(s) 1292
BstX2I RGATCY 2 cut(s) 240, 277
BstXI CCANNNNNNTGG 1 cut(s) 1102
BstYI RGATCY 2 cut(s) 240, 277
BsuRI GGCC 3 cut(s) 30, 172, 320
BtgZI GCGATG 1 cut(s) 117
BtsI GCAGTG 1 cut(s) 1167
BtsIMutI CAGTG 4 cut(s) 60, 687, 1167, 1271
BveI ACCTGC 1 cut(s) 1152
Cac8I GCNNGC 3 cut(s) 334, 577, 1177
CaiI CAGNNNCTG 1 cut(s) 62
CciI TCATGA 1 cut(s) 148
CfoI GCGC 1 cut(s) 380
Cfr13I GGNCC 2 cut(s) 170, 642
CseI GACGC 3 cut(s) 369, 468, 1286
CsiI ACCWGGT 1 cut(s) 409
Csp6I GTAC 2 cut(s) 583, 597
CspCI CAANNNNNGTGG 2 cut(s) 169, 204
CviAII CATG 6 cut(s) 26, 51, 110, 149, 631, 1057
CviQI GTAC 2 cut(s) 583, 597
DdeI CTNAG 7 cut(s) 138, 663, 904, 978, 983, 1024, 1279
DpnI GATC 4 cut(s) 242, 279, 369, 804
DpnII GATC 4 cut(s) 240, 277, 367, 802
DraI TTTAAA 1 cut(s) 1077
EaeI YGGCCR 1 cut(s) 28
Ecl136II GAGCTC 1 cut(s) 1276
Eco24I GRGCYC 1 cut(s) 1278
Eco47I GGWCC 1 cut(s) 642
Eco53kI GAGCTC 1 cut(s) 1276
Eco57I CTGAAG 2 cut(s) 233, 980
Eco91I GGTNACC 3 cut(s) 287, 908, 1258
EcoICRI GAGCTC 1 cut(s) 1276
EcoO109I RGGNCCY 1 cut(s) 642
EcoO65I GGTNACC 3 cut(s) 287, 908, 1258
EcoRII CCWGG 3 cut(s) 93, 172, 409
EcoT38I GRGCYC 1 cut(s) 1278
FaeI CATG 6 cut(s) 29, 54, 113, 152, 634, 1060
FalI AAGNNNNNCTT 2 cut(s) 733, 765
FaqI GGGAC 1 cut(s) 728
FatI CATG 6 cut(s) 25, 50, 109, 148, 630, 1056
Fnu4HI GCNGC 8 cut(s) 330, 525, 660, 670, 840, 1173, 1180, 1211
FriOI GRGCYC 1 cut(s) 1278
Fsp4HI GCNGC 8 cut(s) 330, 525, 660, 670, 840, 1173, 1180, 1211
FspBI CTAG 5 cut(s) 275, 357, 483, 588, 753
GlaI GCGC 1 cut(s) 379
GluI GCNGC 8 cut(s) 330, 525, 660, 670, 840, 1173, 1180, 1211
HaeIII GGCC 3 cut(s) 30, 172, 320
HapII CCGG 2 cut(s) 559, 712
HgaI GACGC 3 cut(s) 369, 468, 1286
HhaI GCGC 1 cut(s) 380
Hin1II CATG 6 cut(s) 29, 54, 113, 152, 634, 1060
Hin6I GCGC 1 cut(s) 378
HinP1I GCGC 1 cut(s) 378
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HindIII AAGCTT 2 cut(s) 18, 820
HinfI GANTC 1 cut(s) 1088
HpaII CCGG 2 cut(s) 559, 712
HphI GGTGA 4 cut(s) 442, 920, 1097, 1270
Hpy166II GTNNAC 2 cut(s) 508, 556
Hpy188I TCNGA 3 cut(s) 898, 1012, 1027
Hpy188III TCNNGA 6 cut(s) 149, 275, 640, 797, 977, 998
Hpy8I GTNNAC 2 cut(s) 508, 556
HpyAV CCTTC 6 cut(s) 331, 347, 514, 614, 794, 1224
HpyCH4III ACNGT 2 cut(s) 726, 931
HpyCH4IV ACGT 1 cut(s) 82
HpyF10VI GCNNNNNNNGC 2 cut(s) 326, 770
HpyF3I CTNAG 7 cut(s) 138, 663, 904, 978, 983, 1024, 1279
HpySE526I ACGT 1 cut(s) 82
Hsp92II CATG 6 cut(s) 29, 54, 113, 152, 634, 1060
HspAI GCGC 1 cut(s) 378
Kzo9I GATC 4 cut(s) 240, 277, 367, 802
LmnI GCTCC 3 cut(s) 656, 1000, 1273
Lsp1109I GCAGC 7 cut(s) 316, 511, 646, 826, 1184, 1191, 1222
LweI GCATC 2 cut(s) 14, 958
MabI ACCWGGT 1 cut(s) 409
MaeI CTAG 5 cut(s) 275, 357, 483, 588, 753
MaeII ACGT 1 cut(s) 82
MaeIII GTNAC 6 cut(s) 58, 78, 287, 908, 1165, 1258
MalI GATC 4 cut(s) 242, 279, 369, 804
MbiI CCGCTC 1 cut(s) 669
MboI GATC 4 cut(s) 240, 277, 367, 802
MboII GAAGA 4 cut(s) 629, 878, 986, 1292
MflI RGATCY 2 cut(s) 240, 277
MhlI GDGCHC 2 cut(s) 510, 1278
MmeI TCCRAC 1 cut(s) 268
MnlI CCTC 7 cut(s) 115, 364, 657, 1062, 1119, 1199, 1314
MseI TTAA 7 cut(s) 534, 567, 825, 861, 870, 1076, 1318
MslI CAYNNNNRTG 1 cut(s) 65
MspI CCGG 2 cut(s) 559, 712
MspR9I CCNGG 5 cut(s) 95, 174, 411, 560, 713
Mva1269I GAATGC 1 cut(s) 104
MvaI CCWGG 3 cut(s) 95, 174, 411
MvnI CGCG 1 cut(s) 380
MwoI GCNNNNNNNGC 2 cut(s) 326, 770
NciI CCSGG 2 cut(s) 560, 713
NdeII GATC 4 cut(s) 240, 277, 367, 802
NlaIII CATG 6 cut(s) 29, 54, 113, 152, 634, 1060
NlaIV GGNNCC 1 cut(s) 644
NmuCI GTSAC 4 cut(s) 58, 908, 1165, 1258
NspI RCATGY 1 cut(s) 54
PagI TCATGA 1 cut(s) 148
PctI GAATGC 1 cut(s) 104
PfeI GAWTC 1 cut(s) 1088
PfoI TCCNGGA 1 cut(s) 93
PkrI GCNGC 8 cut(s) 331, 526, 661, 671, 841, 1174, 1181, 1212
Ppu21I YACGTR 1 cut(s) 83
PpuMI RGGWCCY 1 cut(s) 642
Psp124BI GAGCTC 1 cut(s) 1278
Psp5II RGGWCCY 1 cut(s) 642
Psp6I CCWGG 3 cut(s) 93, 172, 409
PspEI GGTNACC 3 cut(s) 287, 908, 1258
PspGI CCWGG 3 cut(s) 93, 172, 409
PspN4I GGNNCC 1 cut(s) 644
PspPI GGNCC 2 cut(s) 170, 642
PspPPI RGGWCCY 1 cut(s) 642
PsrI GAACNNNNNNTAC 2 cut(s) 25, 57
PstI CTGCAG 1 cut(s) 1174
PstNI CAGNNNCTG 1 cut(s) 62
PsuI RGATCY 2 cut(s) 240, 277
RsaI GTAC 2 cut(s) 584, 598
RsaNI GTAC 2 cut(s) 583, 597
RseI CAYNNNNRTG 1 cut(s) 65
SacI GAGCTC 1 cut(s) 1278
SaqAI TTAA 7 cut(s) 534, 567, 825, 861, 870, 1076, 1318
SatI GCNGC 8 cut(s) 330, 525, 660, 670, 840, 1173, 1180, 1211
Sau3AI GATC 4 cut(s) 240, 277, 367, 802
Sau96I GGNCC 2 cut(s) 170, 642
ScrFI CCNGG 5 cut(s) 95, 174, 411, 560, 713
SduI GDGCHC 2 cut(s) 510, 1278
SexAI ACCWGGT 1 cut(s) 409
SfaNI GCATC 2 cut(s) 14, 958
SfcI CTRYAG 1 cut(s) 1170
SinI GGWCC 1 cut(s) 642
SmiMI CAYNNNNRTG 1 cut(s) 65
SmlI CTYRAG 1 cut(s) 833
SmoI CTYRAG 1 cut(s) 833
SsiI CCGC 3 cut(s) 669, 855, 935
SspI AATATT 1 cut(s) 829
SspMI CTAG 5 cut(s) 275, 357, 483, 588, 753
SstI GAGCTC 1 cut(s) 1278
StyD4I CCNGG 5 cut(s) 93, 172, 409, 558, 711
TaaI ACNGT 2 cut(s) 726, 931
TaiI ACGT 1 cut(s) 85
TaqI TCGA 2 cut(s) 366, 549
TaqII GACCGA 1 cut(s) 939
TatI WGTACW 1 cut(s) 582
TauI GCSGC 1 cut(s) 672
TfiI GAWTC 1 cut(s) 1088
Tru1I TTAA 7 cut(s) 534, 567, 825, 861, 870, 1076, 1318
Tru9I TTAA 7 cut(s) 534, 567, 825, 861, 870, 1076, 1318
TscAI CASTG 4 cut(s) 67, 687, 1174, 1271
TseFI GTSAC 4 cut(s) 58, 908, 1165, 1258
TseI GCWGC 7 cut(s) 329, 524, 659, 839, 1172, 1179, 1210
Tsp45I GTSAC 4 cut(s) 58, 908, 1165, 1258
TspDTI ATGAA 4 cut(s) 14, 137, 776, 1073
TspGWI ACGGA 1 cut(s) 1044
TspRI CASTG 4 cut(s) 67, 687, 1174, 1271
VneI GTGCAC 1 cut(s) 506
VpaK11BI GGWCC 1 cut(s) 642
XapI RAATTY 1 cut(s) 144
XbaI TCTAGA 1 cut(s) 274
XceI RCATGY 1 cut(s) 54
XcmI CCANNNNNNNNNTGG 1 cut(s) 687
XspI CTAG 5 cut(s) 275, 357, 483, 588, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.