pycom01g17340

Sorting and assembly machinery

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
N/A
Physical Location & Seq
Reverse (-)
16669971 .. 16672281
2311 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1128 bp
ATGGAAAAATCCGACGCCGAAACTCAAAACGCTAACGACGACCGCGACGAGCAAACTCCCTCCAATGTCAACGGCACTGTCGACGACGAGCCCGAACCCGACGAAATCGATGACGAAATGGACGAAGAAGAAGACGAAGACGAGGAGGAGGAGCCACCTCTGCCACGAAATCCCCTCTCCCAGGAGGCGCGGTTGCGCGCGGAGAAGTCCAAGGTAGAGAACCTGGTCCGTCAGATGTCGACCGAGCGAGTCGATCTGAGGGTCCACGACGTTCTGATCAAGGGCAATACGAAGACGAAGGAGCATTTGATCGAGGCCGAGCTGGCGGAGATTAAGAAGGCCACCACCATGCAAGAGCTGCTCGAGGCTGCGGCAATTGCAAATGCGAAGCTTCAGAAGCTTGAAATATTCGACGCGGTTCGGATCACGCTCGATTCTGGCCCGCCGGAGCTGCCTGGAACTACGAATGTTATTGTGGAGGTCGTCGAAACTAAGAGTCCAATTTCTGGGGAAATCGGTGCTTACACGAAGCCGGCGGCTAGGTCATGGACTGCTGAAGGAACACTTAAGTTCAAAAATCTGCTTGGATATGGGGATTTGTGGGATGGTTCTTTGGCGTATGGGCCCAACCAAACGTCGGAGTTAAGTGCTGGCCTGTTTATGCCCAGGTTGAAAGGGTTTGTAACTCCAGTGACGGCACGAGCATTCCTGCTTTCCCAAGATTGGATGGAGTTCTCTTCTTATAAAGAGCGCATGATGGGGCTTTCTCTTGGACTGATTTCATCAAAACACCATGATTTGGCATACAACCTGGGTTGGCGAACGCTAGCAGACCCATCACAATTGGCCTCGAGGTCAATAAGAAGGCAGCTCGGCCATGGATTACTTTCTTCAATCAAATACACATTTAAGATTGATAGGAGAAATTCACCTTTGAGGCCAACTCGAGGTTACTCATTTGTTTCTACAAGTCATATTGGTGGCATTGCACCTGATCATCGAAGCACGAGGTTTTTGCGTCAGGAGTTTGATATTCGTTATGCAGTTCCATTAGGATTTTATCATGCTGCACTTAATTTTGGGATCGATCCGCTGGAGTTATTTTTCCATGGGGGTGTGGATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006996 GO:0007005 GO:0007006 GO:0007007 GO:0008150 GO:0009987 GO:0016043 GO:0042407 GO:0061024 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bac_surface_Ag POTRA

Protein Analysis

376

Amino Acids

41.96

Weight (kDa)

4.99

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Omp85 PF01103 193 - 354 8.9e-10 Omp85 superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 744
AbsI CCTCGAGG 1 cut(s) 850
AccB7I CCANNNNNTGG 2 cut(s) 506, 799
AccI GTMKAC 2 cut(s) 81, 239
AccII CGCG 5 cut(s) 45, 190, 198, 200, 416
AciI CCGC 9 cut(s) 43, 190, 200, 326, 371, 416, 443, 536, 1091
AclWI GGATC 3 cut(s) 431, 1082, 1091
AcoI YGGCCR 1 cut(s) 874
AcsI RAATTY 1 cut(s) 925
AcuI CTGAAG 2 cut(s) 377, 576
AcyI GRCGYC 1 cut(s) 15
AfiI CCNNNNNNNGG 6 cut(s) 181, 506, 637, 723, 799, 947
AflII CTTAAG 1 cut(s) 566
AgsI TTSAA 4 cut(s) 404, 574, 673, 894
AjnI CCWGG 5 cut(s) 180, 222, 454, 665, 810
AluBI AGCT 6 cut(s) 322, 358, 391, 400, 451, 871
AluI AGCT 6 cut(s) 322, 358, 391, 400, 451, 871
AlwI GGATC 3 cut(s) 431, 1082, 1091
Ama87I CYCGRG 3 cut(s) 362, 850, 945
AoxI GGCC 8 cut(s) 315, 339, 439, 623, 652, 846, 874, 938
ApaI GGGCCC 1 cut(s) 627
ApeKI GCWGC 5 cut(s) 358, 368, 451, 868, 1067
ApoI RAATTY 1 cut(s) 925
AspLEI GCGC 4 cut(s) 190, 198, 200, 753
AspS9I GGNCC 5 cut(s) 226, 262, 440, 623, 624
AsuHPI GGTGA 1 cut(s) 921
AsuNHI GCTAGC 1 cut(s) 826
AvaI CYCGRG 3 cut(s) 362, 850, 945
AvaII GGWCC 2 cut(s) 226, 262
BaeGI GKGCMC 1 cut(s) 627
BanII GRGCYC 2 cut(s) 93, 627
BauI CACGAG 2 cut(s) 699, 1006
BbsI GAAGAC 3 cut(s) 138, 144, 299
BbvI GCAGC 5 cut(s) 345, 355, 438, 880, 1054
BccI CCATC 4 cut(s) 599, 721, 751, 844
BceAI ACGGC 2 cut(s) 88, 711
BcgI CGANNNNNNTGC 2 cut(s) 997, 1031
BciT130I CCWGG 5 cut(s) 182, 224, 456, 667, 812
BclI TGATCA 2 cut(s) 276, 994
BfaI CTAG 2 cut(s) 540, 827
BfrI CTTAAG 1 cut(s) 566
BglI GCCNNNNNGGC 1 cut(s) 323
BisI GCNGC 7 cut(s) 359, 369, 372, 452, 537, 869, 1068
BlsI GCNGC 7 cut(s) 360, 370, 373, 453, 538, 870, 1069
Bme1390I CCNGG 5 cut(s) 182, 224, 456, 667, 812
Bme18I GGWCC 2 cut(s) 226, 262
BmeT110I CYCGRG 3 cut(s) 362, 850, 945
BmgT120I GGNCC 5 cut(s) 226, 262, 440, 623, 624
BmiI GGNNCC 3 cut(s) 153, 263, 625
BmrFI CCNGG 5 cut(s) 182, 224, 456, 667, 812
BmtI GCTAGC 1 cut(s) 830
BpiI GAAGAC 3 cut(s) 138, 144, 299
BplI GAGNNNNNCTC 2 cut(s) 928, 960
BpmI CTGGAG 2 cut(s) 672, 1115
Bsa29I ATCGAT 2 cut(s) 108, 1086
BsaHI GRCGYC 1 cut(s) 15
BsaJI CCNNGG 6 cut(s) 180, 210, 665, 811, 877, 1108
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 6 cut(s) 181, 506, 637, 723, 799, 947
Bse118I RCCGGY 1 cut(s) 532
Bse1I ACTGG 1 cut(s) 689
Bse3DI GCAATG 1 cut(s) 984
BseBI CCWGG 5 cut(s) 182, 224, 456, 667, 812
BseCI ATCGAT 2 cut(s) 108, 1086
BseDI CCNNGG 6 cut(s) 180, 210, 665, 811, 877, 1108
BseGI GGATG 2 cut(s) 610, 732
BseLI CCNNNNNNNGG 6 cut(s) 181, 506, 637, 723, 799, 947
BseMI GCAATG 1 cut(s) 984
BseMII CTCAG 1 cut(s) 248
BseNI ACTGG 1 cut(s) 689
BsePI GCGCGC 1 cut(s) 196
BseRI GAGGAG 3 cut(s) 158, 161, 164
BseSI GKGCMC 1 cut(s) 627
BseXI GCAGC 5 cut(s) 345, 355, 438, 880, 1054
BsgI GTGCAG 1 cut(s) 1053
Bsh1236I CGCG 5 cut(s) 45, 190, 198, 200, 416
Bsh1285I CGRYCG 2 cut(s) 43, 243
BshFI GGCC 8 cut(s) 317, 341, 441, 625, 654, 848, 876, 940
BshVI ATCGAT 2 cut(s) 108, 1086
BsiEI CGRYCG 2 cut(s) 43, 243
BsiHKCI CYCGRG 3 cut(s) 362, 850, 945
BsiSI CCGG 2 cut(s) 446, 533
BslI CCNNNNNNNGG 6 cut(s) 181, 506, 637, 723, 799, 947
BsmI GAATGC 1 cut(s) 704
BsnI GGCC 8 cut(s) 317, 341, 441, 625, 654, 848, 876, 940
BsoBI CYCGRG 3 cut(s) 362, 850, 945
Bsp120I GGGCCC 1 cut(s) 623
Bsp1286I GDGCHC 2 cut(s) 93, 627
Bsp143I GATC 7 cut(s) 253, 276, 309, 423, 994, 1083, 1087
Bsp19I CCATGG 2 cut(s) 877, 1108
BspACI CCGC 9 cut(s) 43, 190, 200, 326, 371, 416, 443, 536, 1091
BspANI GGCC 8 cut(s) 317, 341, 441, 625, 654, 848, 876, 940
BspCNI CTCAG 1 cut(s) 249
BspDI ATCGAT 2 cut(s) 108, 1086
BspFNI CGCG 5 cut(s) 45, 190, 198, 200, 416
BspLI GGNNCC 3 cut(s) 153, 263, 625
BspOI GCTAGC 1 cut(s) 830
BspPI GGATC 3 cut(s) 431, 1082, 1091
BspTI CTTAAG 1 cut(s) 566
BsrDI GCAATG 1 cut(s) 984
BsrFI RCCGGY 1 cut(s) 532
BsrI ACTGG 1 cut(s) 689
BssAI RCCGGY 1 cut(s) 532
BssECI CCNNGG 6 cut(s) 180, 210, 665, 811, 877, 1108
BssHII GCGCGC 1 cut(s) 196
BssMI GATC 7 cut(s) 253, 276, 309, 423, 994, 1083, 1087
BssNI GRCGYC 1 cut(s) 15
BssSI CACGAG 2 cut(s) 699, 1006
BssT1I CCWWGG 3 cut(s) 210, 877, 1108
Bst2BI CACGAG 2 cut(s) 699, 1006
Bst2UI CCWGG 5 cut(s) 182, 224, 456, 667, 812
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 1 cut(s) 742
BstACI GRCGYC 1 cut(s) 15
BstAFI CTTAAG 1 cut(s) 566
BstAPI GCANNNNNTGC 1 cut(s) 358
BstC8I GCNNGC 6 cut(s) 198, 324, 443, 534, 652, 828
BstDEI CTNAG 2 cut(s) 257, 492
BstDSI CCRYGG 2 cut(s) 877, 1108
BstENI CCTNNNNNAGG 1 cut(s) 179
BstF5I GGATG 2 cut(s) 610, 732
BstFNI CGCG 5 cut(s) 45, 190, 198, 200, 416
BstHHI GCGC 4 cut(s) 190, 198, 200, 753
BstKTI GATC 7 cut(s) 256, 279, 312, 426, 997, 1086, 1090
BstMBI GATC 7 cut(s) 253, 276, 309, 423, 994, 1083, 1087
BstMCI CGRYCG 2 cut(s) 43, 243
BstMWI GCNNNNNNNGC 6 cut(s) 160, 323, 358, 377, 397, 451
BstNI CCWGG 5 cut(s) 182, 224, 456, 667, 812
BstSCI CCNGG 5 cut(s) 180, 222, 454, 665, 810
BstSLI GKGCMC 1 cut(s) 627
BstUI CGCG 5 cut(s) 45, 190, 198, 200, 416
BstV1I GCAGC 5 cut(s) 345, 355, 438, 880, 1054
BstV2I GAAGAC 3 cut(s) 138, 144, 299
Bsu15I ATCGAT 2 cut(s) 108, 1086
BsuRI GGCC 8 cut(s) 317, 341, 441, 625, 654, 848, 876, 940
BsuTUI ATCGAT 2 cut(s) 108, 1086
BtgI CCRYGG 2 cut(s) 877, 1108
BtsCI GGATG 2 cut(s) 610, 732
BtsIMutI CAGTG 2 cut(s) 75, 696
Cac8I GCNNGC 6 cut(s) 198, 324, 443, 534, 652, 828
CfoI GCGC 4 cut(s) 190, 198, 200, 753
Cfr10I RCCGGY 1 cut(s) 532
Cfr13I GGNCC 5 cut(s) 226, 262, 440, 623, 624
ClaI ATCGAT 2 cut(s) 108, 1086
CseI GACGC 3 cut(s) 23, 422, 1007
CsiI ACCWGGT 1 cut(s) 222
CviAII CATG 7 cut(s) 349, 546, 754, 794, 878, 1064, 1109
DdeI CTNAG 2 cut(s) 257, 492
DpnI GATC 7 cut(s) 255, 278, 311, 425, 996, 1085, 1089
DpnII GATC 7 cut(s) 253, 276, 309, 423, 994, 1083, 1087
EaeI YGGCCR 1 cut(s) 874
Eam1104I CTCTTC 1 cut(s) 742
EarI CTCTTC 1 cut(s) 742
EciI GGCGGA 1 cut(s) 341
Eco130I CCWWGG 3 cut(s) 210, 877, 1108
Eco24I GRGCYC 2 cut(s) 93, 627
Eco47I GGWCC 2 cut(s) 226, 262
Eco57I CTGAAG 2 cut(s) 377, 576
Eco88I CYCGRG 3 cut(s) 362, 850, 945
EcoNI CCTNNNNNAGG 1 cut(s) 179
EcoRII CCWGG 5 cut(s) 180, 222, 454, 665, 810
EcoT14I CCWWGG 3 cut(s) 210, 877, 1108
EcoT38I GRGCYC 2 cut(s) 93, 627
ErhI CCWWGG 3 cut(s) 210, 877, 1108
FaeI CATG 7 cut(s) 352, 549, 757, 797, 881, 1067, 1112
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FatI CATG 7 cut(s) 348, 545, 753, 793, 877, 1063, 1108
FauI CCCGC 1 cut(s) 450
FbaI TGATCA 2 cut(s) 276, 994
FblI GTMKAC 2 cut(s) 81, 239
Fnu4HI GCNGC 7 cut(s) 359, 369, 372, 452, 537, 869, 1068
FokI GGATG 2 cut(s) 617, 739
FriOI GRGCYC 2 cut(s) 93, 627
Fsp4HI GCNGC 7 cut(s) 359, 369, 372, 452, 537, 869, 1068
FspBI CTAG 2 cut(s) 540, 827
GlaI GCGC 4 cut(s) 189, 197, 199, 752
GluI GCNGC 7 cut(s) 359, 369, 372, 452, 537, 869, 1068
GsuI CTGGAG 2 cut(s) 672, 1115
HaeIII GGCC 8 cut(s) 317, 341, 441, 625, 654, 848, 876, 940
HapII CCGG 2 cut(s) 446, 533
HgaI GACGC 3 cut(s) 23, 422, 1007
HhaI GCGC 4 cut(s) 190, 198, 200, 753
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 7 cut(s) 352, 549, 757, 797, 881, 1067, 1112
Hin6I GCGC 4 cut(s) 188, 196, 198, 751
HinP1I GCGC 4 cut(s) 188, 196, 198, 751
HincII GTYRAC 3 cut(s) 70, 82, 240
HindII GTYRAC 3 cut(s) 70, 82, 240
HindIII AAGCTT 2 cut(s) 389, 398
HinfI GANTC 4 cut(s) 249, 434, 496, 1121
HpaII CCGG 2 cut(s) 446, 533
HphI GGTGA 1 cut(s) 921
Hpy166II GTNNAC 4 cut(s) 70, 82, 240, 265
Hpy188I TCNGA 7 cut(s) 13, 234, 258, 276, 396, 423, 640
Hpy188III TCNNGA 2 cut(s) 1022, 1125
Hpy8I GTNNAC 4 cut(s) 70, 82, 240, 265
HpyAV CCTTC 4 cut(s) 292, 331, 551, 858
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 2 cut(s) 270, 635
HpyCH4V TGCA 5 cut(s) 352, 380, 989, 1043, 1070
HpyF10VI GCNNNNNNNGC 6 cut(s) 160, 323, 358, 377, 397, 451
HpyF3I CTNAG 2 cut(s) 257, 492
HpySE526I ACGT 2 cut(s) 270, 635
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 7 cut(s) 352, 549, 757, 797, 881, 1067, 1112
HspAI GCGC 4 cut(s) 188, 196, 198, 751
KroI GCCGGC 1 cut(s) 532
KroNI GCCGGC 1 cut(s) 534
Ksp22I TGATCA 2 cut(s) 276, 994
Kzo9I GATC 7 cut(s) 253, 276, 309, 423, 994, 1083, 1087
LmnI GCTCC 3 cut(s) 151, 301, 448
Lsp1109I GCAGC 5 cut(s) 345, 355, 438, 880, 1054
MabI ACCWGGT 1 cut(s) 222
MaeI CTAG 2 cut(s) 540, 827
MaeII ACGT 2 cut(s) 270, 635
MaeIII GTNAC 3 cut(s) 682, 691, 950
MalI GATC 7 cut(s) 255, 278, 311, 425, 996, 1085, 1089
MboI GATC 7 cut(s) 253, 276, 309, 423, 994, 1083, 1087
MboII GAAGA 7 cut(s) 137, 140, 143, 149, 304, 729, 882
MfeI CAATTG 2 cut(s) 375, 842
MhlI GDGCHC 2 cut(s) 93, 627
MluCI AATT 5 cut(s) 375, 501, 842, 925, 1075
MlyI GAGTC 2 cut(s) 258, 505
MmeI TCCRAC 2 cut(s) 36, 618
MroNI GCCGGC 1 cut(s) 532
MseI TTAA 5 cut(s) 333, 567, 644, 909, 1074
MslI CAYNNNNRTG 3 cut(s) 347, 978, 1113
MspA1I CMGCKG 1 cut(s) 1093
MspCI CTTAAG 1 cut(s) 566
MspI CCGG 2 cut(s) 446, 533
MspR9I CCNGG 5 cut(s) 182, 224, 456, 667, 812
MunI CAATTG 2 cut(s) 375, 842
Mva1269I GAATGC 1 cut(s) 704
MvaI CCWGG 5 cut(s) 182, 224, 456, 667, 812
MvnI CGCG 5 cut(s) 45, 190, 198, 200, 416
MwoI GCNNNNNNNGC 6 cut(s) 160, 323, 358, 377, 397, 451
NaeI GCCGGC 1 cut(s) 534
NcoI CCATGG 2 cut(s) 877, 1108
NdeII GATC 7 cut(s) 253, 276, 309, 423, 994, 1083, 1087
NgoMIV GCCGGC 1 cut(s) 532
NheI GCTAGC 1 cut(s) 826
NlaIII CATG 7 cut(s) 352, 549, 757, 797, 881, 1067, 1112
NlaIV GGNNCC 3 cut(s) 153, 263, 625
NmeAIII GCCGAG 2 cut(s) 343, 852
NmuCI GTSAC 1 cut(s) 691
PaeR7I CTCGAG 3 cut(s) 362, 850, 945
PauI GCGCGC 1 cut(s) 196
PcsI WCGNNNNNNNCGW 6 cut(s) 36, 42, 45, 78, 90, 120
PctI GAATGC 1 cut(s) 704
PdiI GCCGGC 1 cut(s) 534
PfeI GAWTC 2 cut(s) 434, 1121
PflMI CCANNNNNTGG 2 cut(s) 506, 799
PkrI GCNGC 7 cut(s) 360, 370, 373, 453, 538, 870, 1069
PleI GAGTC 2 cut(s) 257, 504
PpsI GAGTC 2 cut(s) 257, 504
PsiI TTATAA 1 cut(s) 744
Psp6I CCWGG 5 cut(s) 180, 222, 454, 665, 810
PspGI CCWGG 5 cut(s) 180, 222, 454, 665, 810
PspN4I GGNNCC 3 cut(s) 153, 263, 625
PspOMI GGGCCC 1 cut(s) 623
PspPI GGNCC 5 cut(s) 226, 262, 440, 623, 624
PspXI VCTCGAGB 3 cut(s) 362, 850, 945
PteI GCGCGC 1 cut(s) 196
RseI CAYNNNNRTG 3 cut(s) 347, 978, 1113
SalI GTCGAC 2 cut(s) 80, 238
SaqAI TTAA 5 cut(s) 333, 567, 644, 909, 1074
SatI GCNGC 7 cut(s) 359, 369, 372, 452, 537, 869, 1068
Sau3AI GATC 7 cut(s) 253, 276, 309, 423, 994, 1083, 1087
Sau96I GGNCC 5 cut(s) 226, 262, 440, 623, 624
SchI GAGTC 2 cut(s) 258, 505
ScrFI CCNGG 5 cut(s) 182, 224, 456, 667, 812
SduI GDGCHC 2 cut(s) 93, 627
SexAI ACCWGGT 1 cut(s) 222
Sfr274I CTCGAG 3 cut(s) 362, 850, 945
SinI GGWCC 2 cut(s) 226, 262
SlaI CTCGAG 3 cut(s) 362, 850, 945
SmiMI CAYNNNNRTG 3 cut(s) 347, 978, 1113
SmlI CTYRAG 4 cut(s) 362, 566, 850, 945
SmoI CTYRAG 4 cut(s) 362, 566, 850, 945
Sse9I AATT 5 cut(s) 375, 501, 842, 925, 1075
SsiI CCGC 9 cut(s) 43, 190, 200, 326, 371, 416, 443, 536, 1091
SspI AATATT 1 cut(s) 408
SspMI CTAG 2 cut(s) 540, 827
StyD4I CCNGG 5 cut(s) 180, 222, 454, 665, 810
StyI CCWWGG 3 cut(s) 210, 877, 1108
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 2 cut(s) 273, 638
TaqII GACCGA 1 cut(s) 257
TasI AATT 5 cut(s) 375, 501, 842, 925, 1075
TauI GCSGC 2 cut(s) 374, 539
TfiI GAWTC 2 cut(s) 434, 1121
Tru1I TTAA 5 cut(s) 333, 567, 644, 909, 1074
Tru9I TTAA 5 cut(s) 333, 567, 644, 909, 1074
TscAI CASTG 2 cut(s) 82, 696
TseFI GTSAC 1 cut(s) 691
TseI GCWGC 5 cut(s) 358, 368, 451, 868, 1067
Tsp45I GTSAC 1 cut(s) 691
TspDTI ATGAA 1 cut(s) 771
TspGWI ACGGA 1 cut(s) 218
TspRI CASTG 2 cut(s) 82, 696
Van91I CCANNNNNTGG 2 cut(s) 506, 799
Vha464I CTTAAG 1 cut(s) 566
VpaK11BI GGWCC 2 cut(s) 226, 262
XagI CCTNNNNNAGG 1 cut(s) 179
XapI RAATTY 1 cut(s) 925
XhoI CTCGAG 3 cut(s) 362, 850, 945
XmiI GTMKAC 2 cut(s) 81, 239
XspI CTAG 2 cut(s) 540, 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.