pycom01g17960

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
N/A
Physical Location & Seq
Forward (+)
17109721 .. 17111078
1358 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1209 bp
ATGGCCAGATATACATCCCCGAGATCAATTGGATGCTCATCGATCCTCTGCATTGCAGTGACCATTGGATTTAGAGACACAAAACACCTGGGAAATGCTTCTGGGCTAGCAGTGATGACAGTTATGTTGGTTACCACATGCCTCACTTCCTTGGTGATTATCATTTGTTGGCACAAGCCGCCTATTGTAGCCCTTTGCTTCCTAATCTTCTTTGGCTCCATTGAATTGCTCTACTTCTCAGCTTCGCTCACCAAGTTCCGAGAGGGTGCCTGGCTTCCCATCCTTCTTGCGCTGTTCCTCATGACTATCATGTTTGTATGGCACTATGGAACCATCAAGAAATACGAATATGACCTCCACAACAAGGTTTCATTAGACTGGCTTCTAGCTTTGGGTCCAAGCTTGGGAACTGCTCGGGTCCCTGGCATTGGCTTAGTGTTCACAGATCTCACCTCCGGCATTCCTGCTAACTTCTCCCGCTTTGTAACCAACCTCCCTGCTTTCCACCGCGTCCTAGTTTTCGTGTGTGTAAAATCAGTCCCAGTCCCTTATGTGCCGCCTGATGAGCGATACTTGGTGGGTCGCGTTGGTCCTGCAGCTCATCGGTCCTACAGGTGCATTGTTCGTTATGGATACCGTGATGTTCATCAAGATGTTGACTCCTTTGAAACAGAACTAGTTGAAAGGCTGGTTGATTTCATCCGATATGATTGGTGTCGAACACAGAGAAACAGCTCATGCACCGAGGATGATGCATCAAGGTCTACTGACACGAGCGAGTGTAGACTAGCTGTCATCGGAACAGTAGCACTTTCTGGTGCACCAGCTTATGAGATTGAGGAGACTCAACCGGCAAGTGTATCTGCTGGTTTCCCAACTATAGAAAGTATAGCAGAAGTTATTGAGATGGAACCGGTTGAAAGAAGAGTGAGGTTTGTTATCGACGAGGAGTCTAAGCCTGACACCCGGCCTGAGAATACAATGCAGATGTGCGAAGAGCTTGAGGATCTCTACGCAGCTCAACAGGCTGGGACTGCATTTATACTTGGGCACTCACACGTTAAAGCCAAGCAAGGATCGTCCTTTATGAAGAGATTCGCCATTAACTTCGGTTATAACTGCCTGAGGAGGAATTGCAGGGGGCCAGACGTGGCGCTGAAGGTCCCTCCCGTGTCTCTTCTCGAGGTTGGCATGGTTTATATTGTGTAG

Protein Analysis

403

Amino Acids

44.92

Weight (kDa)

6.51

Isoelectric Point (pI)

44.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K_trans PF02705 15 - 129 7.3e-29 K+ potassium transporter integral membrane domain
K_trans_C PF22776 141 - 400 7.5e-30 K+ potassium transporter C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1116
AccB1I GGYRCC 1 cut(s) 266
AccI GTMKAC 2 cut(s) 764, 784
AccII CGCG 2 cut(s) 510, 585
AciI CCGC 4 cut(s) 179, 478, 508, 557
AclWI GGATC 3 cut(s) 37, 1014, 1084
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 1178
AfiI CCNNNNNNNGG 3 cut(s) 364, 404, 428
AflIII ACRYGT 1 cut(s) 1057
AgeI ACCGGT 1 cut(s) 913
AgsI TTSAA 4 cut(s) 224, 668, 683, 920
AhdI GACNNNNNGTC 2 cut(s) 791, 949
AhlI ACTAGT 1 cut(s) 676
AjiI CACGTC 1 cut(s) 1150
AjnI CCWGG 3 cut(s) 87, 269, 421
AluBI AGCT 9 cut(s) 242, 389, 402, 599, 735, 791, 827, 1000, 1019
AluI AGCT 9 cut(s) 242, 389, 402, 599, 735, 791, 827, 1000, 1019
Alw21I GWGCWC 1 cut(s) 823
Alw26I GTCTC 3 cut(s) 69, 836, 1179
Alw44I GTGCAC 1 cut(s) 819
AlwI GGATC 3 cut(s) 37, 1014, 1084
Ama87I CYCGRG 3 cut(s) 19, 414, 1181
AoxI GGCC 3 cut(s) 3, 968, 1142
ApaLI GTGCAC 1 cut(s) 819
ApeKI GCWGC 2 cut(s) 596, 1016
AsiGI ACCGGT 1 cut(s) 913
Asp700I GAANNNNTTC 2 cut(s) 97, 1094
AspLEI GCGC 2 cut(s) 292, 1156
AspS9I GGNCC 6 cut(s) 395, 418, 590, 606, 1142, 1162
AsuC2I CCSGG 1 cut(s) 967
AsuHPI GGTGA 3 cut(s) 166, 241, 442
AsuNHI GCTAGC 1 cut(s) 106
AvaI CYCGRG 3 cut(s) 19, 414, 1181
AvaII GGWCC 5 cut(s) 395, 418, 590, 606, 1162
AxyI CCTNAGG 1 cut(s) 1124
BaeGI GKGCMC 2 cut(s) 823, 1053
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 266
BauI CACGAG 1 cut(s) 772
Bbv12I GWGCWC 1 cut(s) 823
BbvI GCAGC 2 cut(s) 608, 1028
BccI CCATC 3 cut(s) 287, 341, 901
BcgI CGANNNNNNTGC 2 cut(s) 734, 768
BciT130I CCWGG 3 cut(s) 89, 271, 423
BciVI GTATCC 1 cut(s) 626
BcnI CCSGG 1 cut(s) 967
BcoDI GTCTC 3 cut(s) 69, 836, 1179
BcuI ACTAGT 1 cut(s) 676
BfaI CTAG 5 cut(s) 107, 386, 515, 677, 788
BfmI CTRYAG 3 cut(s) 594, 610, 879
BfoI RGCGCY 1 cut(s) 1157
BfuI GTATCC 1 cut(s) 626
BglII AGATCT 1 cut(s) 445
BisI GCNGC 4 cut(s) 179, 557, 597, 1017
BlsI GCNGC 4 cut(s) 180, 558, 598, 1018
Bme1390I CCNGG 4 cut(s) 89, 271, 423, 967
Bme18I GGWCC 5 cut(s) 395, 418, 590, 606, 1162
BmeRI GACNNNNNGTC 2 cut(s) 791, 949
BmeT110I CYCGRG 3 cut(s) 19, 414, 1181
BmgBI CACGTC 1 cut(s) 1150
BmgT120I GGNCC 6 cut(s) 395, 418, 590, 606, 1142, 1162
BmiI GGNNCC 9 cut(s) 217, 268, 331, 396, 419, 420, 912, 1143, 1164
BmrFI CCNGG 4 cut(s) 89, 271, 423, 967
BmrI ACTGGG 1 cut(s) 536
BmsI GCATC 3 cut(s) 23, 742, 764
BmtI GCTAGC 1 cut(s) 110
BmuI ACTGGG 1 cut(s) 536
BpuEI CTTGAG 1 cut(s) 1022
BpuMI CCSGG 1 cut(s) 967
Bsa29I ATCGAT 1 cut(s) 41
BsaBI GATNNNNATC 3 cut(s) 13, 37, 645
BsaJI CCNNGG 4 cut(s) 88, 150, 421, 744
BsaWI WCCGGW 1 cut(s) 913
Bsc4I CCNNNNNNNGG 3 cut(s) 364, 404, 428
Bse118I RCCGGY 2 cut(s) 850, 913
Bse1I ACTGG 2 cut(s) 383, 542
Bse21I CCTNAGG 1 cut(s) 1124
Bse3DI GCAATG 1 cut(s) 51
Bse8I GATNNNNATC 3 cut(s) 13, 37, 645
BseBI CCWGG 3 cut(s) 89, 271, 423
BseCI ATCGAT 1 cut(s) 41
BseDI CCNNGG 4 cut(s) 88, 150, 421, 744
BseGI GGATG 5 cut(s) 14, 38, 279, 699, 754
BseJI GATNNNNATC 3 cut(s) 13, 37, 645
BseLI CCNNNNNNNGG 3 cut(s) 364, 404, 428
BseMI GCAATG 1 cut(s) 51
BseMII CTCAG 3 cut(s) 252, 963, 1115
BseNI ACTGG 2 cut(s) 383, 542
BseRI GAGGAG 3 cut(s) 854, 962, 1141
BseSI GKGCMC 2 cut(s) 823, 1053
BseXI GCAGC 2 cut(s) 608, 1028
BseYI CCCAGC 1 cut(s) 1028
Bsh1236I CGCG 2 cut(s) 510, 585
BshFI GGCC 3 cut(s) 5, 970, 1144
BshNI GGYRCC 1 cut(s) 266
BshTI ACCGGT 1 cut(s) 913
BshVI ATCGAT 1 cut(s) 41
BsiHKAI GWGCWC 1 cut(s) 823
BsiHKCI CYCGRG 3 cut(s) 19, 414, 1181
BsiSI CCGG 4 cut(s) 456, 851, 914, 967
BslFI GGGAC 5 cut(s) 404, 524, 530, 1045, 1148
BslI CCNNNNNNNGG 3 cut(s) 364, 404, 428
BsmAI GTCTC 3 cut(s) 69, 836, 1179
BsmFI GGGAC 5 cut(s) 404, 524, 530, 1045, 1148
BsmI GAATGC 1 cut(s) 459
BsnI GGCC 3 cut(s) 5, 970, 1144
BsoBI CYCGRG 3 cut(s) 19, 414, 1181
Bsp1286I GDGCHC 2 cut(s) 823, 1053
Bsp143I GATC 5 cut(s) 23, 42, 445, 1006, 1076
BspACI CCGC 4 cut(s) 179, 478, 508, 557
BspANI GGCC 3 cut(s) 5, 970, 1144
BspCNI CTCAG 3 cut(s) 251, 964, 1116
BspDI ATCGAT 1 cut(s) 41
BspFNI CGCG 2 cut(s) 510, 585
BspHI TCATGA 1 cut(s) 300
BspLI GGNNCC 9 cut(s) 217, 268, 331, 396, 419, 420, 912, 1143, 1164
BspMAI CTGCAG 1 cut(s) 598
BspOI GCTAGC 1 cut(s) 110
BspPI GGATC 3 cut(s) 37, 1014, 1084
BspQI GCTCTTC 1 cut(s) 990
BspT107I GGYRCC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 51
BsrFI RCCGGY 2 cut(s) 850, 913
BsrI ACTGG 2 cut(s) 383, 542
BssAI RCCGGY 2 cut(s) 850, 913
BssECI CCNNGG 4 cut(s) 88, 150, 421, 744
BssMI GATC 5 cut(s) 23, 42, 445, 1006, 1076
BssSI CACGAG 1 cut(s) 772
BssT1I CCWWGG 1 cut(s) 150
Bst2BI CACGAG 1 cut(s) 772
Bst2UI CCWGG 3 cut(s) 89, 271, 423
Bst4CI ACNGT 3 cut(s) 121, 638, 805
Bst6I CTCTTC 4 cut(s) 919, 990, 1085, 1182
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 5 cut(s) 238, 433, 954, 972, 1124
BstEII GGTNACC 1 cut(s) 130
BstF5I GGATG 5 cut(s) 14, 38, 279, 699, 754
BstFNI CGCG 2 cut(s) 510, 585
BstH2I RGCGCY 1 cut(s) 1157
BstHHI GCGC 2 cut(s) 292, 1156
BstKTI GATC 5 cut(s) 26, 45, 448, 1009, 1079
BstMAI GTCTC 3 cut(s) 69, 836, 1179
BstMBI GATC 5 cut(s) 23, 42, 445, 1006, 1076
BstMWI GCNNNNNNNGC 4 cut(s) 178, 565, 1025, 1034
BstNI CCWGG 3 cut(s) 89, 271, 423
BstNSI RCATGY 1 cut(s) 141
BstPI GGTNACC 1 cut(s) 130
BstSCI CCNGG 4 cut(s) 87, 269, 421, 965
BstSFI CTRYAG 3 cut(s) 594, 610, 879
BstSLI GKGCMC 2 cut(s) 823, 1053
BstUI CGCG 2 cut(s) 510, 585
BstV1I GCAGC 2 cut(s) 608, 1028
BstX2I RGATCY 2 cut(s) 445, 1006
BstYI RGATCY 2 cut(s) 445, 1006
Bsu15I ATCGAT 1 cut(s) 41
Bsu36I CCTNAGG 1 cut(s) 1124
BsuI GTATCC 1 cut(s) 626
BsuRI GGCC 3 cut(s) 5, 970, 1144
BsuTUI ATCGAT 1 cut(s) 41
BtrI CACGTC 1 cut(s) 1150
BtsCI GGATG 5 cut(s) 14, 38, 279, 699, 754
BtsI GCAGTG 2 cut(s) 63, 117
BtsIMutI CAGTG 2 cut(s) 63, 117
Cac8I GCNNGC 1 cut(s) 108
CciI TCATGA 1 cut(s) 300
CfoI GCGC 2 cut(s) 292, 1156
Cfr10I RCCGGY 2 cut(s) 850, 913
Cfr13I GGNCC 6 cut(s) 395, 418, 590, 606, 1142, 1162
ClaI ATCGAT 1 cut(s) 41
CseI GACGC 1 cut(s) 499
CspAI ACCGGT 1 cut(s) 913
CviAII CATG 5 cut(s) 138, 301, 310, 738, 1192
DdeI CTNAG 5 cut(s) 238, 433, 954, 972, 1124
DpnI GATC 5 cut(s) 25, 44, 447, 1008, 1078
DpnII GATC 5 cut(s) 23, 42, 445, 1006, 1076
DriI GACNNNNNGTC 2 cut(s) 791, 949
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 919, 990, 1085, 1182
Eam1105I GACNNNNNGTC 2 cut(s) 791, 949
EarI CTCTTC 4 cut(s) 919, 990, 1085, 1182
Eco130I CCWWGG 1 cut(s) 150
Eco47I GGWCC 5 cut(s) 395, 418, 590, 606, 1162
Eco57I CTGAAG 1 cut(s) 1178
Eco81I CCTNAGG 1 cut(s) 1124
Eco88I CYCGRG 3 cut(s) 19, 414, 1181
Eco91I GGTNACC 1 cut(s) 130
EcoO109I RGGNCCY 2 cut(s) 418, 1162
EcoO65I GGTNACC 1 cut(s) 130
EcoRII CCWGG 3 cut(s) 87, 269, 421
EcoT14I CCWWGG 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 757
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 5 cut(s) 141, 304, 313, 741, 1195
FaqI GGGAC 5 cut(s) 404, 524, 530, 1045, 1148
FatI CATG 5 cut(s) 137, 300, 309, 737, 1191
FauI CCCGC 1 cut(s) 485
FblI GTMKAC 2 cut(s) 764, 784
Fnu4HI GCNGC 4 cut(s) 179, 557, 597, 1017
FokI GGATG 4 cut(s) 45, 266, 686, 761
Fsp4HI GCNGC 4 cut(s) 179, 557, 597, 1017
FspBI CTAG 5 cut(s) 107, 386, 515, 677, 788
GlaI GCGC 2 cut(s) 291, 1155
GluI GCNGC 4 cut(s) 179, 557, 597, 1017
GsaI CCCAGC 1 cut(s) 1032
HaeII RGCGCY 1 cut(s) 1157
HaeIII GGCC 3 cut(s) 5, 970, 1144
HapII CCGG 4 cut(s) 456, 851, 914, 967
HgaI GACGC 1 cut(s) 499
HhaI GCGC 2 cut(s) 292, 1156
Hin1II CATG 5 cut(s) 141, 304, 313, 741, 1195
Hin6I GCGC 2 cut(s) 290, 1154
HinP1I GCGC 2 cut(s) 290, 1154
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HindIII AAGCTT 1 cut(s) 400
HinfI GANTC 4 cut(s) 659, 844, 950, 1095
HpaII CCGG 4 cut(s) 456, 851, 914, 967
HphI GGTGA 3 cut(s) 166, 241, 442
Hpy166II GTNNAC 5 cut(s) 441, 658, 765, 785, 821
Hpy188I TCNGA 3 cut(s) 260, 704, 800
Hpy188III TCNNGA 4 cut(s) 301, 337, 650, 1181
Hpy8I GTNNAC 5 cut(s) 441, 658, 765, 785, 821
Hpy99I CGWCG 1 cut(s) 947
HpyAV CCTTC 2 cut(s) 293, 1153
HpyCH4III ACNGT 3 cut(s) 121, 638, 805
HpyCH4IV ACGT 2 cut(s) 1059, 1149
HpyF10VI GCNNNNNNNGC 4 cut(s) 178, 565, 1025, 1034
HpyF3I CTNAG 5 cut(s) 238, 433, 954, 972, 1124
HpySE526I ACGT 2 cut(s) 1059, 1149
Hsp92II CATG 5 cut(s) 141, 304, 313, 741, 1195
HspAI GCGC 2 cut(s) 290, 1154
KflI GGGWCCC 1 cut(s) 418
Kzo9I GATC 5 cut(s) 23, 42, 445, 1006, 1076
LguI GCTCTTC 1 cut(s) 990
LmnI GCTCC 1 cut(s) 221
Lsp1109I GCAGC 2 cut(s) 608, 1028
LweI GCATC 3 cut(s) 23, 742, 764
MaeI CTAG 5 cut(s) 107, 386, 515, 677, 788
MaeII ACGT 2 cut(s) 1059, 1149
MaeIII GTNAC 3 cut(s) 58, 130, 484
MalI GATC 5 cut(s) 25, 44, 447, 1008, 1078
MboI GATC 5 cut(s) 23, 42, 445, 1006, 1076
MboII GAAGA 5 cut(s) 199, 936, 1007, 1102, 1169
MfeI CAATTG 1 cut(s) 27
MflI RGATCY 2 cut(s) 445, 1006
MhlI GDGCHC 2 cut(s) 823, 1053
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 27, 224, 1132
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 653, 838, 959
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 757
MroXI GAANNNNTTC 2 cut(s) 97, 1094
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 1062, 1104
MslI CAYNNNNRTG 2 cut(s) 56, 651
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 4 cut(s) 456, 851, 914, 967
MspR9I CCNGG 4 cut(s) 89, 271, 423, 967
MunI CAATTG 1 cut(s) 27
Mva1269I GAATGC 1 cut(s) 459
MvaI CCWGG 3 cut(s) 89, 271, 423
MvnI CGCG 2 cut(s) 510, 585
MwoI GCNNNNNNNGC 4 cut(s) 178, 565, 1025, 1034
NciI CCSGG 1 cut(s) 967
NdeII GATC 5 cut(s) 23, 42, 445, 1006, 1076
NheI GCTAGC 1 cut(s) 106
NlaIII CATG 5 cut(s) 141, 304, 313, 741, 1195
NlaIV GGNNCC 9 cut(s) 217, 268, 331, 396, 419, 420, 912, 1143, 1164
NmuCI GTSAC 1 cut(s) 58
NsiI ATGCAT 1 cut(s) 757
NspI RCATGY 1 cut(s) 141
PaeR7I CTCGAG 1 cut(s) 1181
PagI TCATGA 1 cut(s) 300
PciSI GCTCTTC 1 cut(s) 990
PctI GAATGC 1 cut(s) 459
PdmI GAANNNNTTC 2 cut(s) 97, 1094
PfeI GAWTC 1 cut(s) 1095
PinAI ACCGGT 1 cut(s) 913
PkrI GCNGC 4 cut(s) 180, 558, 598, 1018
PleI GAGTC 3 cut(s) 653, 838, 958
PpsI GAGTC 3 cut(s) 653, 838, 958
PpuMI RGGWCCY 2 cut(s) 418, 1162
PsiI TTATAA 1 cut(s) 1116
Psp5II RGGWCCY 2 cut(s) 418, 1162
Psp6I CCWGG 3 cut(s) 87, 269, 421
PspEI GGTNACC 1 cut(s) 130
PspFI CCCAGC 1 cut(s) 1028
PspGI CCWGG 3 cut(s) 87, 269, 421
PspN4I GGNNCC 9 cut(s) 217, 268, 331, 396, 419, 420, 912, 1143, 1164
PspPI GGNCC 6 cut(s) 395, 418, 590, 606, 1142, 1162
PspPPI RGGWCCY 2 cut(s) 418, 1162
PstI CTGCAG 1 cut(s) 598
PsuI RGATCY 2 cut(s) 445, 1006
RseI CAYNNNNRTG 2 cut(s) 56, 651
SapI GCTCTTC 1 cut(s) 990
SaqAI TTAA 2 cut(s) 1062, 1104
SatI GCNGC 4 cut(s) 179, 557, 597, 1017
Sau3AI GATC 5 cut(s) 23, 42, 445, 1006, 1076
Sau96I GGNCC 6 cut(s) 395, 418, 590, 606, 1142, 1162
SchI GAGTC 3 cut(s) 653, 838, 959
ScrFI CCNGG 4 cut(s) 89, 271, 423, 967
SduI GDGCHC 2 cut(s) 823, 1053
SfaNI GCATC 3 cut(s) 23, 742, 764
SfcI CTRYAG 3 cut(s) 594, 610, 879
Sfr274I CTCGAG 1 cut(s) 1181
SinI GGWCC 5 cut(s) 395, 418, 590, 606, 1162
SlaI CTCGAG 1 cut(s) 1181
SmiMI CAYNNNNRTG 2 cut(s) 56, 651
SmlI CTYRAG 2 cut(s) 1001, 1181
SmoI CTYRAG 2 cut(s) 1001, 1181
SpeI ACTAGT 1 cut(s) 676
Sse9I AATT 3 cut(s) 27, 224, 1132
SsiI CCGC 4 cut(s) 179, 478, 508, 557
SspMI CTAG 5 cut(s) 107, 386, 515, 677, 788
StyD4I CCNGG 4 cut(s) 87, 269, 421, 965
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 3 cut(s) 121, 638, 805
TaiI ACGT 2 cut(s) 1062, 1152
TaqI TCGA 4 cut(s) 41, 718, 942, 1182
TaqII GACCGA 1 cut(s) 594
TasI AATT 3 cut(s) 27, 224, 1132
TauI GCSGC 2 cut(s) 181, 559
TfiI GAWTC 1 cut(s) 1095
Tru1I TTAA 2 cut(s) 1062, 1104
Tru9I TTAA 2 cut(s) 1062, 1104
TscAI CASTG 2 cut(s) 63, 117
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 2 cut(s) 596, 1016
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 4 cut(s) 360, 635, 688, 1103
TspRI CASTG 2 cut(s) 63, 117
VneI GTGCAC 1 cut(s) 819
VpaK11BI GGWCC 5 cut(s) 395, 418, 590, 606, 1162
XceI RCATGY 1 cut(s) 141
XhoI CTCGAG 1 cut(s) 1181
XmiI GTMKAC 2 cut(s) 764, 784
XmnI GAANNNNTTC 2 cut(s) 97, 1094
XspI CTAG 5 cut(s) 107, 386, 515, 677, 788
Zsp2I ATGCAT 1 cut(s) 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.