pycom01g18420

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
N/A
Physical Location & Seq
Forward (+)
17427611 .. 17428865
1255 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 621 bp
ATGGAGGCGGAAATTGATGCCGGTACACCCTTGGACTACGCTGAATTACAGATTTTTCCGTCCCAGAACAGGTATGAGGCCCTTGTTTCCAGTGGCAGGGAAGTGGAAAAGTTAGCAGGTGGGGCACTTGAACCGTTGTTGCCACATTTTCCGGAAGTAAGTGAATTGCATTGCAAGGGATCAAATGCTAACCTCACACTCCGACTTCCTAAAAGCCTGCATGGTGCTGCCTGGTTCACGAAATCAACTTTAACCAGATTCCTGCAAATTACTGGTTCACCAGACGTAATGCATACCATTACTGCTACTGAAGGAGAAATATCTCAGTTGGAAGAAGCCAAAAAGTTTCATGTTTCTTTATATGGCCAGCCTGAAGCTGAGATTGCATCATCAGATGCTTCAAAGAACGAACTTTTGCGGGCACTGGACTTGAGGCTCACTGCATTAAGAAGGGAATTAACGATTGCTATTGAAAAGGCTTGTCATTCCTCGTTCAACTCTAAAGAGATTTCAAATGTAGCAAACTTTTCTCAACATTTTGGAACAACAGATTTCAGGAATACTCTGTTCAAGATTTTAGAACAGTATCAACAAGGCAAGAGTGATCGATCCTCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

207

Amino Acids

22.87

Weight (kDa)

5.82

Isoelectric Point (pI)

49.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 107
Acc36I ACCTGC 1 cut(s) 107
AccIII TCCGGA 1 cut(s) 151
AciI CCGC 2 cut(s) 8, 418
AclWI GGATC 2 cut(s) 187, 603
AcoI YGGCCR 1 cut(s) 364
AcuI CTGAAG 2 cut(s) 330, 393
AfaI GTAC 1 cut(s) 25
AfiI CCNNNNNNNGG 2 cut(s) 69, 96
AgsI TTSAA 6 cut(s) 131, 402, 473, 496, 513, 571
AjnI CCWGG 1 cut(s) 230
AluBI AGCT 1 cut(s) 377
AluI AGCT 1 cut(s) 377
AlwI GGATC 2 cut(s) 187, 603
Aor13HI TCCGGA 1 cut(s) 151
AoxI GGCC 2 cut(s) 78, 364
ApeKI GCWGC 1 cut(s) 227
AspS9I GGNCC 1 cut(s) 79
AsuHPI GGTGA 1 cut(s) 270
BaeGI GKGCMC 2 cut(s) 127, 424
BalI TGGCCA 1 cut(s) 366
BbvI GCAGC 1 cut(s) 214
BciT130I CCWGG 1 cut(s) 232
BfuAI ACCTGC 1 cut(s) 107
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
Bme1390I CCNGG 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 79
BmrFI CCNGG 1 cut(s) 232
BmsI GCATC 3 cut(s) 7, 385, 395
BpuEI CTTGAG 1 cut(s) 451
Bsa29I ATCGAT 1 cut(s) 607
BsaJI CCNNGG 1 cut(s) 30
BsaWI WCCGGW 1 cut(s) 151
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 96
Bse118I RCCGGY 1 cut(s) 20
Bse1I ACTGG 3 cut(s) 90, 277, 429
Bse3DI GCAATG 1 cut(s) 169
BseAI TCCGGA 1 cut(s) 151
BseBI CCWGG 1 cut(s) 232
BseCI ATCGAT 1 cut(s) 607
BseDI CCNNGG 1 cut(s) 30
BseLI CCNNNNNNNGG 2 cut(s) 69, 96
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 2 cut(s) 338, 369
BseNI ACTGG 3 cut(s) 90, 277, 429
BseSI GKGCMC 2 cut(s) 127, 424
BseXI GCAGC 1 cut(s) 214
BshFI GGCC 2 cut(s) 80, 366
BshVI ATCGAT 1 cut(s) 607
BsiSI CCGG 2 cut(s) 21, 152
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 2 cut(s) 69, 96
BsmFI GGGAC 1 cut(s) 46
BsnI GGCC 2 cut(s) 80, 366
Bsp1286I GDGCHC 2 cut(s) 127, 424
Bsp13I TCCGGA 1 cut(s) 151
Bsp143I GATC 3 cut(s) 179, 604, 608
BspACI CCGC 2 cut(s) 8, 418
BspANI GGCC 2 cut(s) 80, 366
BspCNI CTCAG 2 cut(s) 337, 370
BspDI ATCGAT 1 cut(s) 607
BspEI TCCGGA 1 cut(s) 151
BspMI ACCTGC 1 cut(s) 107
BspPI GGATC 2 cut(s) 187, 603
BsrDI GCAATG 1 cut(s) 169
BsrFI RCCGGY 1 cut(s) 20
BsrI ACTGG 3 cut(s) 90, 277, 429
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 3 cut(s) 179, 604, 608
BssT1I CCWWGG 1 cut(s) 30
Bst2UI CCWGG 1 cut(s) 232
Bst4CI ACNGT 2 cut(s) 135, 585
BstC8I GCNNGC 3 cut(s) 218, 368, 420
BstDEI CTNAG 2 cut(s) 324, 378
BstKTI GATC 3 cut(s) 182, 607, 611
BstMBI GATC 3 cut(s) 179, 604, 608
BstMWI GCNNNNNNNGC 2 cut(s) 122, 383
BstNI CCWGG 1 cut(s) 232
BstSCI CCNGG 1 cut(s) 230
BstSLI GKGCMC 2 cut(s) 127, 424
BstV1I GCAGC 1 cut(s) 214
Bsu15I ATCGAT 1 cut(s) 607
BsuRI GGCC 2 cut(s) 80, 366
BsuTUI ATCGAT 1 cut(s) 607
BtsI GCAGTG 1 cut(s) 438
BtsIMutI CAGTG 3 cut(s) 97, 422, 438
BveI ACCTGC 1 cut(s) 107
Cac8I GCNNGC 3 cut(s) 218, 368, 420
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 1 cut(s) 79
ClaI ATCGAT 1 cut(s) 607
Csp6I GTAC 1 cut(s) 24
CviAII CATG 2 cut(s) 221, 350
CviJI RGCY 8 cut(s) 80, 216, 338, 366, 370, 377, 436, 479
CviKI_1 RGCY 8 cut(s) 80, 216, 338, 366, 370, 377, 436, 479
CviQI GTAC 1 cut(s) 24
DdeI CTNAG 2 cut(s) 324, 378
DpnI GATC 3 cut(s) 181, 606, 610
DpnII GATC 3 cut(s) 179, 604, 608
EaeI YGGCCR 1 cut(s) 364
EciI GGCGGA 1 cut(s) 23
Eco130I CCWWGG 1 cut(s) 30
Eco57I CTGAAG 2 cut(s) 330, 393
EcoO109I RGGNCCY 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 230
EcoT14I CCWWGG 1 cut(s) 30
EcoT22I ATGCAT 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 2 cut(s) 224, 353
FaiI YATR 6 cut(s) 75, 222, 294, 351, 361, 363
FaqI GGGAC 1 cut(s) 46
FatI CATG 2 cut(s) 220, 349
FauI CCCGC 1 cut(s) 411
Fnu4HI GCNGC 1 cut(s) 228
Fsp4HI GCNGC 1 cut(s) 228
GluI GCNGC 1 cut(s) 228
HaeIII GGCC 2 cut(s) 80, 366
HapII CCGG 2 cut(s) 21, 152
Hin1II CATG 2 cut(s) 224, 353
HinfI GANTC 1 cut(s) 258
HpaII CCGG 2 cut(s) 21, 152
HphI GGTGA 1 cut(s) 270
Hpy166II GTNNAC 3 cut(s) 26, 237, 278
Hpy188I TCNGA 2 cut(s) 203, 394
Hpy188III TCNNGA 4 cut(s) 152, 238, 556, 571
Hpy8I GTNNAC 3 cut(s) 26, 237, 278
HpyAV CCTTC 2 cut(s) 305, 444
HpyCH4III ACNGT 2 cut(s) 135, 585
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 7 cut(s) 169, 174, 220, 265, 292, 386, 443
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 383
HpyF3I CTNAG 2 cut(s) 324, 378
HpySE526I ACGT 1 cut(s) 285
Hsp92II CATG 2 cut(s) 224, 353
Kpn2I TCCGGA 1 cut(s) 151
Kzo9I GATC 3 cut(s) 179, 604, 608
Lsp1109I GCAGC 1 cut(s) 214
LweI GCATC 3 cut(s) 7, 385, 395
MaeII ACGT 1 cut(s) 285
MalI GATC 3 cut(s) 181, 606, 610
MboI GATC 3 cut(s) 179, 604, 608
MboII GAAGA 1 cut(s) 344
MhlI GDGCHC 2 cut(s) 127, 424
MlsI TGGCCA 1 cut(s) 366
MluCI AATT 5 cut(s) 12, 44, 164, 267, 455
MluNI TGGCCA 1 cut(s) 366
MmeI TCCRAC 2 cut(s) 226, 309
MnlI CCTC 4 cut(s) 70, 203, 426, 499
Mox20I TGGCCA 1 cut(s) 366
Mph1103I ATGCAT 1 cut(s) 294
MroI TCCGGA 1 cut(s) 151
MscI TGGCCA 1 cut(s) 366
MseI TTAA 3 cut(s) 251, 446, 458
Msp20I TGGCCA 1 cut(s) 366
MspI CCGG 2 cut(s) 21, 152
MspR9I CCNGG 1 cut(s) 232
MvaI CCWGG 1 cut(s) 232
MwoI GCNNNNNNNGC 2 cut(s) 122, 383
NdeII GATC 3 cut(s) 179, 604, 608
NlaIII CATG 2 cut(s) 224, 353
NsiI ATGCAT 1 cut(s) 294
PaqCI CACCTGC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 258
PkrI GCNGC 1 cut(s) 229
Psp6I CCWGG 1 cut(s) 230
PspGI CCWGG 1 cut(s) 230
PspPI GGNCC 1 cut(s) 79
RsaI GTAC 1 cut(s) 25
RsaNI GTAC 1 cut(s) 24
SaqAI TTAA 3 cut(s) 251, 446, 458
SatI GCNGC 1 cut(s) 228
Sau3AI GATC 3 cut(s) 179, 604, 608
Sau96I GGNCC 1 cut(s) 79
ScrFI CCNGG 1 cut(s) 232
SduI GDGCHC 2 cut(s) 127, 424
SetI ASST 5 cut(s) 74, 121, 195, 288, 379
SfaNI GCATC 3 cut(s) 7, 385, 395
SmlI CTYRAG 1 cut(s) 430
SmoI CTYRAG 1 cut(s) 430
Sse9I AATT 5 cut(s) 12, 44, 164, 267, 455
SsiI CCGC 2 cut(s) 8, 418
StyD4I CCNGG 1 cut(s) 230
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 135, 585
TaiI ACGT 1 cut(s) 288
TaqI TCGA 1 cut(s) 607
TasI AATT 5 cut(s) 12, 44, 164, 267, 455
TfiI GAWTC 1 cut(s) 258
Tru1I TTAA 3 cut(s) 251, 446, 458
Tru9I TTAA 3 cut(s) 251, 446, 458
TscAI CASTG 3 cut(s) 97, 429, 445
TseI GCWGC 1 cut(s) 227
TspDTI ATGAA 1 cut(s) 338
TspGWI ACGGA 1 cut(s) 48
TspRI CASTG 3 cut(s) 97, 429, 445
Zsp2I ATGCAT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.