pycom01g18630

Phosphodiesterase responsible for the U6 snRNA 3' end processing. Acts as an exoribonuclease (RNase) responsible for trimming the poly(U) tract of the last nucleotides in the pre-U6 snRNA molecule, leading to the formation of mature U6 snRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
N/A
Physical Location & Seq
Forward (+)
17532909 .. 17534915
2007 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 747 bp
ATGGAGGCCCTGATCGCCTCGTACGGAAACTCATCGTCGTCGGCCTCAAGCTCCAAGTCACCGGTTCCACCTCCAGATCTGAACAACTCTCAAAACCCGGCCCCAGCTCTGCCTCCGCCACCTCTACCACTTCTCGAAAGCGCCAATTCCTTGGGATTTCTGGACTTGTCACTAAGCGATCGGCCGAGCAGAGTCAGGAACTTCCCTCACGTCAAAGGGAACTACGTAGTATATGTATACATCCCAGTTTATATACCGCCAGCACCGAGGAAAGAGATGGCCTTGTTTTTGAACAAGCTAGATTCTCTGGTACCTGGTCTCCATGCCGTTGACGTTAACGTCCCGCTTGACACTCTGCGGAATGACAAGCACAAGCTCGAACAAGTTGCTTTAGGTAGAAAATTGCATATAAGTCTGGGAAGAACTGTCCCGGTTCGGGTGAACCAGATTGATTCCTTGGTGACAATGCTGCGGCAGAAGCTTCAAATTCAGAGGCGGTATATGACCCGGACCTTTGTGTCGATAGAAGTCATTGCTGCTGGGTTAGCTGAGATAACAAAGCAGATTCAAGCTGTCAATGAGGTGTATAAGCTTCACAATCTTCCTGAATTCTACAAGGTTCCACGCCCTCATATATCGGTAGCTTGGGCATTGGGTGACATAAGCAATCCCCTGAAGAAAGTGTTTGAAGGAGAAAGAAGAAGATCTGCAGTTGGAACGATCATTACAGAAAAGTATTTTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HVSL

Protein Analysis

249

Amino Acids

27.66

Weight (kDa)

9.77

Isoelectric Point (pI)

58.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HVSL PF09749 59 - 229 1.2e-32 Uncharacterised conserved protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 338, 517
Acc65I GGTACC 1 cut(s) 310
AccB1I GGYRCC 1 cut(s) 310
AccI GTMKAC 1 cut(s) 237
AciI CCGC 6 cut(s) 116, 257, 344, 358, 472, 496
AcoI YGGCCR 1 cut(s) 182
AcsI RAATTY 2 cut(s) 486, 608
AcuI CTGAAG 1 cut(s) 695
AfaI GTAC 2 cut(s) 23, 312
AfiI CCNNNNNNNGG 1 cut(s) 436
AgeI ACCGGT 1 cut(s) 61
AgsI TTSAA 4 cut(s) 292, 485, 569, 689
AjiI CACGTC 1 cut(s) 211
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 9 cut(s) 51, 107, 298, 376, 481, 548, 572, 592, 644
AluI AGCT 9 cut(s) 51, 107, 298, 376, 481, 548, 572, 592, 644
Alw26I GTCTC 1 cut(s) 323
AoxI GGCC 5 cut(s) 6, 42, 99, 182, 279
ApeKI GCWGC 2 cut(s) 469, 536
ApoI RAATTY 2 cut(s) 486, 608
AsiGI ACCGGT 1 cut(s) 61
Asp718I GGTACC 1 cut(s) 310
AspLEI GCGC 1 cut(s) 143
AspS9I GGNCC 3 cut(s) 7, 100, 510
AsuC2I CCSGG 3 cut(s) 98, 431, 508
AsuHPI GGTGA 4 cut(s) 51, 451, 472, 668
AvaII GGWCC 1 cut(s) 510
BanI GGYRCC 1 cut(s) 310
BbvI GCAGC 2 cut(s) 456, 523
BccI CCATC 1 cut(s) 271
BceAI ACGGC 1 cut(s) 311
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BciT130I CCWGG 1 cut(s) 315
BcnI CCSGG 3 cut(s) 98, 431, 508
BcoDI GTCTC 1 cut(s) 323
BfaI CTAG 2 cut(s) 299, 745
BfmI CTRYAG 1 cut(s) 708
BfoI RGCGCY 1 cut(s) 144
BglII AGATCT 2 cut(s) 76, 704
BisI GCNGC 3 cut(s) 470, 473, 537
BlsI GCNGC 3 cut(s) 471, 474, 538
Bme1390I CCNGG 4 cut(s) 98, 315, 431, 508
Bme18I GGWCC 1 cut(s) 510
BmgBI CACGTC 1 cut(s) 211
BmgT120I GGNCC 3 cut(s) 7, 100, 510
BmiI GGNNCC 4 cut(s) 66, 102, 312, 621
BmrFI CCNGG 4 cut(s) 98, 315, 431, 508
BmrI ACTGGG 1 cut(s) 239
BmuI ACTGGG 1 cut(s) 239
BpmI CTGGAG 1 cut(s) 57
BpuEI CTTGAG 1 cut(s) 31
BpuMI CCSGG 3 cut(s) 98, 431, 508
BsaAI YACGTR 1 cut(s) 226
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 3 cut(s) 150, 266, 456
BsaWI WCCGGW 1 cut(s) 61
BsaXI ACNNNNNCTCC 2 cut(s) 303, 333
Bsc4I CCNNNNNNNGG 1 cut(s) 436
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 1 cut(s) 245
Bse3DI GCAATG 1 cut(s) 531
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 3 cut(s) 150, 266, 456
BseGI GGATG 1 cut(s) 240
BseLI CCNNNNNNNGG 1 cut(s) 436
BseMI GCAATG 1 cut(s) 531
BseMII CTCAG 1 cut(s) 540
BseNI ACTGG 1 cut(s) 245
BseX3I CGGCCG 1 cut(s) 182
BseXI GCAGC 2 cut(s) 456, 523
BseYI CCCAGC 2 cut(s) 103, 539
Bsh1285I CGRYCG 2 cut(s) 181, 185
BshFI GGCC 5 cut(s) 8, 44, 101, 184, 281
BshNI GGYRCC 1 cut(s) 310
BshTI ACCGGT 1 cut(s) 61
BsiEI CGRYCG 2 cut(s) 181, 185
BsiSI CCGG 4 cut(s) 62, 98, 431, 508
BsiWI CGTACG 1 cut(s) 21
BslFI GGGAC 2 cut(s) 326, 413
BslI CCNNNNNNNGG 1 cut(s) 436
BsmAI GTCTC 1 cut(s) 323
BsmFI GGGAC 2 cut(s) 326, 413
BsnI GGCC 5 cut(s) 8, 44, 101, 184, 281
Bso31I GGTCTC 1 cut(s) 323
Bsp143I GATC 5 cut(s) 12, 76, 178, 704, 720
BspACI CCGC 6 cut(s) 116, 257, 344, 358, 472, 496
BspANI GGCC 5 cut(s) 8, 44, 101, 184, 281
BspCNI CTCAG 1 cut(s) 541
BspLI GGNNCC 4 cut(s) 66, 102, 312, 621
BspMAI CTGCAG 1 cut(s) 712
BspT107I GGYRCC 1 cut(s) 310
BspTNI GGTCTC 1 cut(s) 323
BsrDI GCAATG 1 cut(s) 531
BsrFI RCCGGY 1 cut(s) 61
BsrI ACTGG 1 cut(s) 245
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 3 cut(s) 150, 266, 456
BssMI GATC 5 cut(s) 12, 76, 178, 704, 720
BssNAI GTATAC 1 cut(s) 238
BssT1I CCWWGG 2 cut(s) 150, 456
Bst1107I GTATAC 1 cut(s) 238
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 427
BstBAI YACGTR 1 cut(s) 226
BstC8I GCNNGC 1 cut(s) 261
BstDEI CTNAG 2 cut(s) 173, 549
BstF5I GGATG 1 cut(s) 240
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 5 cut(s) 15, 79, 181, 707, 723
BstMAI GTCTC 1 cut(s) 323
BstMBI GATC 5 cut(s) 12, 76, 178, 704, 720
BstMCI CGRYCG 2 cut(s) 181, 185
BstMWI GCNNNNNNNGC 3 cut(s) 14, 478, 545
BstNI CCWGG 1 cut(s) 315
BstSCI CCNGG 4 cut(s) 96, 313, 429, 506
BstSFI CTRYAG 1 cut(s) 708
BstSNI TACGTA 1 cut(s) 226
BstV1I GCAGC 2 cut(s) 456, 523
BstX2I RGATCY 2 cut(s) 76, 704
BstXI CCANNNNNNTGG 1 cut(s) 151
BstYI RGATCY 2 cut(s) 76, 704
BstZ17I GTATAC 1 cut(s) 238
BstZI CGGCCG 1 cut(s) 182
BsuRI GGCC 5 cut(s) 8, 44, 101, 184, 281
BtrI CACGTC 1 cut(s) 211
BtsCI GGATG 1 cut(s) 240
Cac8I GCNNGC 1 cut(s) 261
CfoI GCGC 1 cut(s) 143
Cfr10I RCCGGY 1 cut(s) 61
Cfr13I GGNCC 3 cut(s) 7, 100, 510
CsiI ACCWGGT 1 cut(s) 313
Csp6I GTAC 2 cut(s) 22, 311
CspAI ACCGGT 1 cut(s) 61
CviAII CATG 1 cut(s) 323
CviQI GTAC 2 cut(s) 22, 311
DdeI CTNAG 2 cut(s) 173, 549
DpnI GATC 5 cut(s) 14, 78, 180, 706, 722
DpnII GATC 5 cut(s) 12, 76, 178, 704, 720
DrdI GACNNNNNNGTC 2 cut(s) 338, 517
DseDI GACNNNNNNGTC 2 cut(s) 338, 517
EaeI YGGCCR 1 cut(s) 182
EagI CGGCCG 1 cut(s) 182
EciI GGCGGA 1 cut(s) 105
EclXI CGGCCG 1 cut(s) 182
Eco105I TACGTA 1 cut(s) 226
Eco130I CCWWGG 2 cut(s) 150, 456
Eco31I GGTCTC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 510
Eco52I CGGCCG 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 695
EcoO109I RGGNCCY 1 cut(s) 7
EcoRI GAATTC 1 cut(s) 608
EcoRII CCWGG 1 cut(s) 313
EcoT14I CCWWGG 2 cut(s) 150, 456
ErhI CCWWGG 2 cut(s) 150, 456
FaeI CATG 1 cut(s) 326
FaqI GGGAC 2 cut(s) 326, 413
FatI CATG 1 cut(s) 322
FauI CCCGC 1 cut(s) 351
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 3 cut(s) 470, 473, 537
FokI GGATG 1 cut(s) 227
Fsp4HI GCNGC 3 cut(s) 470, 473, 537
FspBI CTAG 2 cut(s) 299, 745
GlaI GCGC 1 cut(s) 142
GluI GCNGC 3 cut(s) 470, 473, 537
GsaI CCCAGC 2 cut(s) 107, 543
GsuI CTGGAG 1 cut(s) 57
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 5 cut(s) 8, 44, 101, 184, 281
HapII CCGG 4 cut(s) 62, 98, 431, 508
HhaI GCGC 1 cut(s) 143
Hin1II CATG 1 cut(s) 326
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HincII GTYRAC 2 cut(s) 331, 337
HindII GTYRAC 2 cut(s) 331, 337
HindIII AAGCTT 2 cut(s) 479, 590
HinfI GANTC 4 cut(s) 192, 302, 452, 565
HpaI GTTAAC 1 cut(s) 337
HpaII CCGG 4 cut(s) 62, 98, 431, 508
HphI GGTGA 4 cut(s) 51, 451, 472, 668
Hpy166II GTNNAC 4 cut(s) 238, 331, 337, 442
Hpy188I TCNGA 2 cut(s) 81, 492
Hpy188III TCNNGA 5 cut(s) 74, 134, 161, 196, 605
Hpy8I GTNNAC 4 cut(s) 238, 331, 337, 442
Hpy99I CGWCG 2 cut(s) 40, 43
HpyAV CCTTC 1 cut(s) 683
HpyCH4III ACNGT 1 cut(s) 427
HpyCH4IV ACGT 4 cut(s) 210, 225, 333, 339
HpyCH4V TGCA 2 cut(s) 406, 710
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 478, 545
HpyF3I CTNAG 2 cut(s) 173, 549
HpySE526I ACGT 4 cut(s) 210, 225, 333, 339
Hsp92II CATG 1 cut(s) 326
HspAI GCGC 1 cut(s) 141
KpnI GGTACC 1 cut(s) 314
KspAI GTTAAC 1 cut(s) 337
Kzo9I GATC 5 cut(s) 12, 76, 178, 704, 720
LmnI GCTCC 1 cut(s) 56
Lsp1109I GCAGC 2 cut(s) 456, 523
MabI ACCWGGT 1 cut(s) 313
MaeI CTAG 2 cut(s) 299, 745
MaeII ACGT 4 cut(s) 210, 225, 333, 339
MaeIII GTNAC 4 cut(s) 57, 168, 460, 656
MalI GATC 5 cut(s) 14, 78, 180, 706, 722
MboI GATC 5 cut(s) 12, 76, 178, 704, 720
MboII GAAGA 5 cut(s) 432, 593, 688, 711, 714
MflI RGATCY 2 cut(s) 76, 704
MluCI AATT 4 cut(s) 145, 401, 486, 608
MlyI GAGTC 1 cut(s) 201
MmeI TCCRAC 1 cut(s) 694
MseI TTAA 1 cut(s) 336
MspI CCGG 4 cut(s) 62, 98, 431, 508
MspR9I CCNGG 4 cut(s) 98, 315, 431, 508
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 3 cut(s) 14, 478, 545
NciI CCSGG 3 cut(s) 98, 431, 508
NdeII GATC 5 cut(s) 12, 76, 178, 704, 720
NlaIII CATG 1 cut(s) 326
NlaIV GGNNCC 4 cut(s) 66, 102, 312, 621
NmeAIII GCCGAG 1 cut(s) 210
NmuCI GTSAC 4 cut(s) 57, 168, 460, 656
PfeI GAWTC 3 cut(s) 302, 452, 565
Pfl23II CGTACG 1 cut(s) 21
PinAI ACCGGT 1 cut(s) 61
PkrI GCNGC 3 cut(s) 471, 474, 538
Ple19I CGATCG 1 cut(s) 181
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
Ppu21I YACGTR 1 cut(s) 226
Psp6I CCWGG 1 cut(s) 313
PspFI CCCAGC 2 cut(s) 103, 539
PspGI CCWGG 1 cut(s) 313
PspLI CGTACG 1 cut(s) 21
PspN4I GGNNCC 4 cut(s) 66, 102, 312, 621
PspPI GGNCC 3 cut(s) 7, 100, 510
PsrI GAACNNNNNNTAC 2 cut(s) 709, 741
PstI CTGCAG 1 cut(s) 712
PsuI RGATCY 2 cut(s) 76, 704
PvuI CGATCG 1 cut(s) 181
RsaI GTAC 2 cut(s) 23, 312
RsaNI GTAC 2 cut(s) 22, 311
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 3 cut(s) 470, 473, 537
Sau3AI GATC 5 cut(s) 12, 76, 178, 704, 720
Sau96I GGNCC 3 cut(s) 7, 100, 510
SchI GAGTC 1 cut(s) 201
ScrFI CCNGG 4 cut(s) 98, 315, 431, 508
SexAI ACCWGGT 1 cut(s) 313
SfcI CTRYAG 1 cut(s) 708
SinI GGWCC 1 cut(s) 510
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
SnaBI TACGTA 1 cut(s) 226
Sse9I AATT 4 cut(s) 145, 401, 486, 608
SsiI CCGC 6 cut(s) 116, 257, 344, 358, 472, 496
SspMI CTAG 2 cut(s) 299, 745
StyD4I CCNGG 4 cut(s) 96, 313, 429, 506
StyI CCWWGG 2 cut(s) 150, 456
TaaI ACNGT 1 cut(s) 427
TaiI ACGT 4 cut(s) 213, 228, 336, 342
TaqI TCGA 3 cut(s) 135, 378, 521
TasI AATT 4 cut(s) 145, 401, 486, 608
TauI GCSGC 1 cut(s) 475
TfiI GAWTC 3 cut(s) 302, 452, 565
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TseFI GTSAC 4 cut(s) 57, 168, 460, 656
TseI GCWGC 2 cut(s) 469, 536
Tsp45I GTSAC 4 cut(s) 57, 168, 460, 656
TspGWI ACGGA 1 cut(s) 39
VpaK11BI GGWCC 1 cut(s) 510
XapI RAATTY 2 cut(s) 486, 608
XmiI GTMKAC 1 cut(s) 237
XspI CTAG 2 cut(s) 299, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.