pycom01g20060

regulation of response to stimulus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
N/A
Physical Location & Seq
Forward (+)
18516480 .. 18516821
342 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 342 bp
ATGTCAATGGCTGCTTCTTCCTCTTCCTCATGGAAATACGACGTCTTCCTGAATTTCAGAGGCGAAGACACTCGCAAGATCTTCGTCGGCCATCTCTACAGTGCTCTGCGTCGGAAAGCAATCAACACCTTCATCGACGCCGAAGAGCTCAGAAAAGGCAATGACCTTTCGGAACTCCTTGCGGCTATCGAGTCGTCGAGGCTTTCGATCGTGGTTTTCTCCCAGGACTACGCTTCTTCGACGTGGTGCTTGAAAGAACTGGTCAAAATCCTGGAGTGCATGGATGCAAAGAAGCAGATAGTGGTGCCGATTTTCTACCAAGTTGTGGATCGATCCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_3 LRR_5 LRR_8 NB-ARC TIR

Protein Analysis

114

Amino Acids

12.89

Weight (kDa)

7.75

Isoelectric Point (pI)

61.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 110 2e-36 TIR domain
TIR_2 PF13676 15 - 107 1.6e-15 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 45
AccB1I GGYRCC 1 cut(s) 304
AccB7I CCANNNNNTGG 1 cut(s) 325
AciI CCGC 1 cut(s) 182
AclWI GGATC 2 cut(s) 327, 336
AcoI YGGCCR 1 cut(s) 88
AcsI RAATTY 1 cut(s) 52
AcyI GRCGYC 2 cut(s) 42, 138
AfiI CCNNNNNNNGG 1 cut(s) 325
AgsI TTSAA 1 cut(s) 253
AjiI CACGTC 1 cut(s) 243
AjnI CCWGG 2 cut(s) 222, 270
AluBI AGCT 1 cut(s) 148
AluI AGCT 1 cut(s) 148
Alw21I GWGCWC 2 cut(s) 106, 150
AlwI GGATC 2 cut(s) 327, 336
AoxI GGCC 1 cut(s) 88
ApeKI GCWGC 1 cut(s) 11
ApoI RAATTY 1 cut(s) 52
BanI GGYRCC 1 cut(s) 304
BanII GRGCYC 1 cut(s) 150
BbsI GAAGAC 2 cut(s) 37, 72
Bbv12I GWGCWC 2 cut(s) 106, 150
BccI CCATC 1 cut(s) 99
BcgI CGANNNNNNTGC 2 cut(s) 64, 98
BciT130I CCWGG 2 cut(s) 224, 272
BfmI CTRYAG 1 cut(s) 97
BglII AGATCT 1 cut(s) 78
BisI GCNGC 2 cut(s) 12, 183
BlsI GCNGC 2 cut(s) 13, 184
Bme1390I CCNGG 2 cut(s) 224, 272
BmgBI CACGTC 1 cut(s) 243
BmiI GGNNCC 1 cut(s) 306
BmrFI CCNGG 2 cut(s) 224, 272
BmsI GCATC 1 cut(s) 274
BpiI GAAGAC 2 cut(s) 37, 72
BpmI CTGGAG 1 cut(s) 293
Bsa29I ATCGAT 1 cut(s) 331
BsaHI GRCGYC 2 cut(s) 42, 138
BsaJI CCNNGG 1 cut(s) 222
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse1I ACTGG 1 cut(s) 264
Bse3DI GCAATG 1 cut(s) 166
BseBI CCWGG 2 cut(s) 224, 272
BseCI ATCGAT 1 cut(s) 331
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 1 cut(s) 325
BseMI GCAATG 1 cut(s) 166
BseMII CTCAG 1 cut(s) 163
BseNI ACTGG 1 cut(s) 264
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 1 cut(s) 90
BshNI GGYRCC 1 cut(s) 304
BshVI ATCGAT 1 cut(s) 331
BsiEI CGRYCG 1 cut(s) 210
BsiHKAI GWGCWC 2 cut(s) 106, 150
BslI CCNNNNNNNGG 1 cut(s) 325
BsnI GGCC 1 cut(s) 90
Bsp1286I GDGCHC 2 cut(s) 106, 150
Bsp143I GATC 4 cut(s) 78, 207, 328, 332
BspACI CCGC 1 cut(s) 182
BspANI GGCC 1 cut(s) 90
BspCNI CTCAG 1 cut(s) 162
BspDI ATCGAT 1 cut(s) 331
BspLI GGNNCC 1 cut(s) 306
BspPI GGATC 2 cut(s) 327, 336
BspQI GCTCTTC 1 cut(s) 138
BspT107I GGYRCC 1 cut(s) 304
BsrDI GCAATG 1 cut(s) 166
BsrI ACTGG 1 cut(s) 264
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 4 cut(s) 78, 207, 328, 332
BssNI GRCGYC 2 cut(s) 42, 138
Bst2UI CCWGG 2 cut(s) 224, 272
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 2 cut(s) 28, 138
BstACI GRCGYC 2 cut(s) 42, 138
BstDEI CTNAG 1 cut(s) 149
BstF5I GGATG 1 cut(s) 289
BstKTI GATC 4 cut(s) 81, 210, 331, 335
BstMBI GATC 4 cut(s) 78, 207, 328, 332
BstMCI CGRYCG 1 cut(s) 210
BstNI CCWGG 2 cut(s) 224, 272
BstSCI CCNGG 2 cut(s) 222, 270
BstSFI CTRYAG 1 cut(s) 97
BstV2I GAAGAC 2 cut(s) 37, 72
BstX2I RGATCY 1 cut(s) 78
BstYI RGATCY 1 cut(s) 78
Bsu15I ATCGAT 1 cut(s) 331
BsuRI GGCC 1 cut(s) 90
BsuTUI ATCGAT 1 cut(s) 331
BtrI CACGTC 1 cut(s) 243
BtsCI GGATG 1 cut(s) 289
BtsIMutI CAGTG 1 cut(s) 106
ClaI ATCGAT 1 cut(s) 331
CseI GACGC 2 cut(s) 98, 146
CviAII CATG 2 cut(s) 30, 280
CviJI RGCY 5 cut(s) 11, 90, 148, 185, 202
CviKI_1 RGCY 5 cut(s) 11, 90, 148, 185, 202
DdeI CTNAG 1 cut(s) 149
DpnI GATC 4 cut(s) 80, 209, 330, 334
DpnII GATC 4 cut(s) 78, 207, 328, 332
EaeI YGGCCR 1 cut(s) 88
Eam1104I CTCTTC 2 cut(s) 28, 138
EarI CTCTTC 2 cut(s) 28, 138
Ecl136II GAGCTC 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 150
Eco53kI GAGCTC 1 cut(s) 148
EcoICRI GAGCTC 1 cut(s) 148
EcoRII CCWGG 2 cut(s) 222, 270
EcoT38I GRGCYC 1 cut(s) 150
FaeI CATG 2 cut(s) 33, 283
FaiI YATR 2 cut(s) 31, 281
FatI CATG 2 cut(s) 29, 279
Fnu4HI GCNGC 2 cut(s) 12, 183
FokI GGATG 1 cut(s) 296
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 2 cut(s) 12, 183
GluI GCNGC 2 cut(s) 12, 183
GsuI CTGGAG 1 cut(s) 293
HaeIII GGCC 1 cut(s) 90
HgaI GACGC 2 cut(s) 98, 146
Hin1I GRCGYC 2 cut(s) 42, 138
Hin1II CATG 2 cut(s) 33, 283
HinfI GANTC 1 cut(s) 191
Hpy188I TCNGA 5 cut(s) 59, 114, 152, 172, 341
Hpy188III TCNNGA 1 cut(s) 49
Hpy99I CGWCG 6 cut(s) 44, 89, 114, 140, 199, 244
HpyAV CCTTC 1 cut(s) 139
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 2 cut(s) 42, 242
HpyCH4V TGCA 2 cut(s) 279, 287
HpyF3I CTNAG 1 cut(s) 149
HpySE526I ACGT 2 cut(s) 42, 242
Hsp92I GRCGYC 2 cut(s) 42, 138
Hsp92II CATG 2 cut(s) 33, 283
Kzo9I GATC 4 cut(s) 78, 207, 328, 332
LguI GCTCTTC 1 cut(s) 138
LpnPI CCDG 6 cut(s) 62, 209, 236, 245, 257, 284
LweI GCATC 1 cut(s) 274
MaeII ACGT 2 cut(s) 42, 242
MalI GATC 4 cut(s) 80, 209, 330, 334
MboI GATC 4 cut(s) 78, 207, 328, 332
MboII GAAGA 7 cut(s) 9, 15, 37, 73, 77, 155, 228
MflI RGATCY 1 cut(s) 78
MhlI GDGCHC 2 cut(s) 106, 150
MluCI AATT 1 cut(s) 52
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 92
MnlI CCTC 4 cut(s) 31, 37, 53, 192
MspR9I CCNGG 2 cut(s) 224, 272
MvaI CCWGG 2 cut(s) 224, 272
NdeII GATC 4 cut(s) 78, 207, 328, 332
NlaIII CATG 2 cut(s) 33, 283
NlaIV GGNNCC 1 cut(s) 306
PciSI GCTCTTC 1 cut(s) 138
PcsI WCGNNNNNNNCGW 1 cut(s) 203
PflMI CCANNNNNTGG 1 cut(s) 325
PfoI TCCNGGA 1 cut(s) 270
PkrI GCNGC 2 cut(s) 13, 184
Ple19I CGATCG 1 cut(s) 210
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
Psp124BI GAGCTC 1 cut(s) 150
Psp6I CCWGG 2 cut(s) 222, 270
PspGI CCWGG 2 cut(s) 222, 270
PspN4I GGNNCC 1 cut(s) 306
PsuI RGATCY 1 cut(s) 78
PvuI CGATCG 1 cut(s) 210
SacI GAGCTC 1 cut(s) 150
SapI GCTCTTC 1 cut(s) 138
SatI GCNGC 2 cut(s) 12, 183
Sau3AI GATC 4 cut(s) 78, 207, 328, 332
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 2 cut(s) 224, 272
SduI GDGCHC 2 cut(s) 106, 150
SetI ASST 5 cut(s) 45, 131, 150, 168, 245
SfaNI GCATC 1 cut(s) 274
SfcI CTRYAG 1 cut(s) 97
Sse9I AATT 1 cut(s) 52
SsiI CCGC 1 cut(s) 182
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 2 cut(s) 222, 270
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 2 cut(s) 45, 245
TaqI TCGA 6 cut(s) 135, 189, 197, 206, 239, 331
TasI AATT 1 cut(s) 52
TauI GCSGC 1 cut(s) 185
TscAI CASTG 1 cut(s) 106
TseI GCWGC 1 cut(s) 11
TspDTI ATGAA 1 cut(s) 121
TspRI CASTG 1 cut(s) 106
Van91I CCANNNNNTGG 1 cut(s) 325
XapI RAATTY 1 cut(s) 52
ZraI GACGTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.