pycom01g20950

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
N/A
Physical Location & Seq
Reverse (-)
19219938 .. 19220393
456 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 456 bp
ATGATCAAGAAACGAAGAAGATTTCTTGAAGAAACAATGGAATGCGATGACTCGCCTATGACTTTGGGAAGATGGGAAGACCTCAATGCTGACATATTGTTGAAGATTTTTGAGTGCTTTGCCAACATCTCCGATTCATCTTCCGCCATCAGAAAGGTTAGCGGTAGCGCCGTTTGCAAGGAGTGGCGTTCGACGCTGTGTGATCCTCGGCTTTGGAACAAGCTTGATTTTTCGACGATGAAATCGCATTTCATCAAAACCCCAGATAAACCTTACGTTTATGTTTGCAGCCGGTCCGAGATGACACCGACTCGTGTTTTGAAGATTTCTCTGAGTCTCAGCAAGATTTATAACATGCATGCACATGCGGGACAACAACTCGTCGTGCATCATGTGCATGAGGAACAGAAACGACCACGGGTTCATCAGGTGGCGGTAGGCATGAAGAAGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

152

Amino Acids

17.49

Weight (kDa)

9.4

Isoelectric Point (pI)

57.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 351
AciI CCGC 4 cut(s) 144, 162, 368, 434
AclWI GGATC 1 cut(s) 197
AgsI TTSAA 3 cut(s) 29, 103, 322
AluBI AGCT 1 cut(s) 223
AluI AGCT 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 341
AlwI GGATC 1 cut(s) 197
ApeKI GCWGC 1 cut(s) 288
ArsI GACNNNNNNTTYG 2 cut(s) 301, 333
AspLEI GCGC 1 cut(s) 170
AspS9I GGNCC 1 cut(s) 294
AvaII GGWCC 1 cut(s) 294
BauI CACGAG 1 cut(s) 312
BbsI GAAGAC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 300
BccI CCATC 2 cut(s) 66, 155
BceAI ACGGC 1 cut(s) 155
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 341
BfoI RGCGCY 1 cut(s) 171
BisI GCNGC 1 cut(s) 289
BlsI GCNGC 1 cut(s) 290
Bme18I GGWCC 1 cut(s) 294
BmgT120I GGNCC 1 cut(s) 294
BmsI GCATC 1 cut(s) 397
BpiI GAAGAC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 206, 416
Bse118I RCCGGY 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 206, 416
BseMII CTCAG 2 cut(s) 323, 352
BseXI GCAGC 1 cut(s) 300
BsiSI CCGG 1 cut(s) 292
BslFI GGGAC 1 cut(s) 384
BsmAI GTCTC 1 cut(s) 341
BsmFI GGGAC 1 cut(s) 384
BsmI GAATGC 1 cut(s) 47
Bsp143I GATC 2 cut(s) 3, 202
BspACI CCGC 4 cut(s) 144, 162, 368, 434
BspCNI CTCAG 2 cut(s) 324, 351
BspPI GGATC 1 cut(s) 197
BsrFI RCCGGY 1 cut(s) 291
BssAI RCCGGY 1 cut(s) 291
BssECI CCNNGG 2 cut(s) 206, 416
BssMI GATC 2 cut(s) 3, 202
BssSI CACGAG 1 cut(s) 312
Bst2BI CACGAG 1 cut(s) 312
BstAPI GCANNNNNTGC 1 cut(s) 394
BstC8I GCNNGC 1 cut(s) 360
BstDEI CTNAG 2 cut(s) 332, 338
BstDSI CCRYGG 1 cut(s) 416
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 2 cut(s) 6, 205
BstMAI GTCTC 1 cut(s) 341
BstMBI GATC 2 cut(s) 3, 202
BstMWI GCNNNNNNNGC 3 cut(s) 174, 193, 394
BstNSI RCATGY 3 cut(s) 358, 362, 368
BstV1I GCAGC 1 cut(s) 300
BstV2I GAAGAC 1 cut(s) 84
BtgI CCRYGG 1 cut(s) 416
BtgZI GCGATG 1 cut(s) 60
Cac8I GCNNGC 1 cut(s) 360
CfoI GCGC 1 cut(s) 170
Cfr10I RCCGGY 1 cut(s) 291
Cfr13I GGNCC 1 cut(s) 294
CpoI CGGWCCG 1 cut(s) 294
CseI GACGC 1 cut(s) 202
CspI CGGWCCG 1 cut(s) 294
CviAII CATG 6 cut(s) 355, 359, 365, 392, 398, 442
CviJI RGCY 3 cut(s) 211, 223, 291
CviKI_1 RGCY 3 cut(s) 211, 223, 291
DdeI CTNAG 2 cut(s) 332, 338
DpnI GATC 2 cut(s) 5, 204
DpnII GATC 2 cut(s) 3, 202
EciI GGCGGA 1 cut(s) 133
Eco47I GGWCC 1 cut(s) 294
EcoT22I ATGCAT 1 cut(s) 360
FaeI CATG 6 cut(s) 358, 362, 368, 395, 401, 445
FaqI GGGAC 1 cut(s) 384
FatI CATG 6 cut(s) 354, 358, 364, 391, 397, 441
FauI CCCGC 1 cut(s) 361
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 289
Fsp4HI GCNGC 1 cut(s) 289
GlaI GCGC 1 cut(s) 169
GluI GCNGC 1 cut(s) 289
HaeII RGCGCY 1 cut(s) 171
HapII CCGG 1 cut(s) 292
HgaI GACGC 1 cut(s) 202
HhaI GCGC 1 cut(s) 170
Hin1II CATG 6 cut(s) 358, 362, 368, 395, 401, 445
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HindIII AAGCTT 1 cut(s) 221
HinfI GANTC 4 cut(s) 50, 134, 310, 334
HpaII CCGG 1 cut(s) 292
Hpy188I TCNGA 4 cut(s) 133, 152, 298, 333
Hpy188III TCNNGA 2 cut(s) 7, 26
Hpy99I CGWCG 3 cut(s) 196, 238, 386
HpyAV CCTTC 1 cut(s) 442
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 6 cut(s) 177, 288, 358, 362, 388, 397
HpyF10VI GCNNNNNNNGC 3 cut(s) 174, 193, 394
HpyF3I CTNAG 2 cut(s) 332, 338
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 6 cut(s) 358, 362, 368, 395, 401, 445
HspAI GCGC 1 cut(s) 168
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 202
LpnPI CCDG 3 cut(s) 276, 305, 413
Lsp1109I GCAGC 1 cut(s) 300
LweI GCATC 1 cut(s) 397
MaeII ACGT 1 cut(s) 276
MalI GATC 2 cut(s) 5, 204
MboI GATC 2 cut(s) 3, 202
MboII GAAGA 8 cut(s) 27, 30, 41, 81, 89, 115, 132, 334
MlyI GAGTC 3 cut(s) 44, 304, 343
MnlI CCTC 3 cut(s) 92, 216, 394
Mph1103I ATGCAT 1 cut(s) 360
MslI CAYNNNNRTG 2 cut(s) 363, 396
MspI CCGG 1 cut(s) 292
Mva1269I GAATGC 1 cut(s) 47
MwoI GCNNNNNNNGC 3 cut(s) 174, 193, 394
NdeII GATC 2 cut(s) 3, 202
NlaIII CATG 6 cut(s) 358, 362, 368, 395, 401, 445
NmeAIII GCCGAG 1 cut(s) 187
NsiI ATGCAT 1 cut(s) 360
NspI RCATGY 3 cut(s) 358, 362, 368
PaeI GCATGC 1 cut(s) 362
PctI GAATGC 1 cut(s) 47
PfeI GAWTC 1 cut(s) 134
PkrI GCNGC 1 cut(s) 290
PleI GAGTC 3 cut(s) 44, 304, 342
PpsI GAGTC 3 cut(s) 44, 304, 342
PsiI TTATAA 1 cut(s) 351
PspPI GGNCC 1 cut(s) 294
RseI CAYNNNNRTG 2 cut(s) 363, 396
Rsr2I CGGWCCG 1 cut(s) 294
RsrII CGGWCCG 1 cut(s) 294
SatI GCNGC 1 cut(s) 289
Sau3AI GATC 2 cut(s) 3, 202
Sau96I GGNCC 1 cut(s) 294
SchI GAGTC 3 cut(s) 44, 304, 343
SetI ASST 6 cut(s) 84, 159, 225, 274, 279, 432
SfaNI GCATC 1 cut(s) 397
SinI GGWCC 1 cut(s) 294
SmiMI CAYNNNNRTG 2 cut(s) 363, 396
SphI GCATGC 1 cut(s) 362
SsiI CCGC 4 cut(s) 144, 162, 368, 434
TaiI ACGT 1 cut(s) 279
TaqI TCGA 2 cut(s) 191, 233
TfiI GAWTC 1 cut(s) 134
TseI GCWGC 1 cut(s) 288
TspDTI ATGAA 4 cut(s) 126, 241, 254, 413
VpaK11BI GGWCC 1 cut(s) 294
XceI RCATGY 3 cut(s) 358, 362, 368
Zsp2I ATGCAT 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.