pycom01g21250

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
N/A
Physical Location & Seq
Reverse (-)
19393429 .. 19394779
1351 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 624 bp
ATGGAGCTTCATTTGCTGGAGATGCAGTTGTTAATAACAGGAGGGCCAAAGATTTGGGAAGCAATCATCCTTGATCTTCTGCCGAAAGGTCTAGGACAAGCGCGTAAGGCTCTTTTATCAGGTTGTTCTGCTGGTGATTTAGCATCATTTTTGCATTGTAACAACTTCACCAAATACTTACCAAGCAACGCCAGTGTAAAATACTTGAGCGACGCCGGATTCTTTCTCGATGAACGAGATGTAAGCTTGGAACCACACCATGTTATCTTTCGTGAAAGATCTTGTGACTCTACAGGGAGTAGAGAAGAATCTGGAGGAGAACTGCACCAAGAATTCCGTTTATCTCGAGCTGTGTTTCTTCCCACAGTATGCACTGAGATTCATCACAACACATTTCTTCATATTGAATTCTCAGCCTATGACGTTTACCAGTTCCATCACATACTGGTTCCGCCTTCAGCTGATCCGCGCGGACATTGGAACCGTTGCAAATTGAATCCTGCAGCATGCAATCCTGACCAGCTAAATACATTGCAAGAATTCAGGCACGATATGATTGTTCTACAAGTATTCCAGAAGAGGAGGGATTTTCCTCAATTCATGCTTTGCTCATTGCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
PAE

Protein Analysis

208

Amino Acids

23.64

Weight (kDa)

6.29

Isoelectric Point (pI)

51.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 15 - 187 4.7e-44 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 103, 469, 471
AciI CCGC 3 cut(s) 452, 467, 471
AclWI GGATC 1 cut(s) 458
AcsI RAATTY 3 cut(s) 332, 407, 539
AcuI CTGAAG 1 cut(s) 441
AcyI GRCGYC 1 cut(s) 213
AgsI TTSAA 2 cut(s) 407, 496
AluBI AGCT 5 cut(s) 7, 246, 350, 461, 523
AluI AGCT 5 cut(s) 7, 246, 350, 461, 523
AlwI GGATC 1 cut(s) 458
Ama87I CYCGRG 1 cut(s) 345
AoxI GGCC 1 cut(s) 44
ApeKI GCWGC 1 cut(s) 503
ApoI RAATTY 3 cut(s) 332, 407, 539
AspLEI GCGC 2 cut(s) 103, 471
AspS9I GGNCC 1 cut(s) 44
AsuHPI GGTGA 2 cut(s) 146, 160
AvaI CYCGRG 1 cut(s) 345
BbvI GCAGC 1 cut(s) 515
BccI CCATC 1 cut(s) 444
BfaI CTAG 1 cut(s) 92
BfmI CTRYAG 2 cut(s) 291, 501
BglII AGATCT 1 cut(s) 278
BisI GCNGC 1 cut(s) 504
BlsI GCNGC 1 cut(s) 505
BmeT110I CYCGRG 1 cut(s) 345
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 3 cut(s) 252, 450, 482
BmsI GCATC 2 cut(s) 12, 152
BpmI CTGGAG 2 cut(s) 38, 333
BpuEI CTTGAG 1 cut(s) 226
BsaHI GRCGYC 1 cut(s) 213
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bse1I ACTGG 3 cut(s) 192, 430, 450
Bse3DI GCAATG 2 cut(s) 530, 611
BseGI GGATG 1 cut(s) 66
BseMI GCAATG 2 cut(s) 530, 611
BseMII CTCAG 2 cut(s) 366, 426
BseNI ACTGG 3 cut(s) 192, 430, 450
BseRI GAGGAG 2 cut(s) 330, 595
BseXI GCAGC 1 cut(s) 515
BsgI GTGCAG 1 cut(s) 308
Bsh1236I CGCG 3 cut(s) 103, 469, 471
BshFI GGCC 1 cut(s) 46
BsiHKCI CYCGRG 1 cut(s) 345
BsiSI CCGG 1 cut(s) 216
BsnI GGCC 1 cut(s) 46
BsoBI CYCGRG 1 cut(s) 345
Bsp143I GATC 3 cut(s) 73, 278, 463
BspACI CCGC 3 cut(s) 452, 467, 471
BspANI GGCC 1 cut(s) 46
BspCNI CTCAG 2 cut(s) 367, 425
BspFNI CGCG 3 cut(s) 103, 469, 471
BspLI GGNNCC 3 cut(s) 252, 450, 482
BspMAI CTGCAG 1 cut(s) 505
BspPI GGATC 1 cut(s) 458
BsrDI GCAATG 2 cut(s) 530, 611
BsrI ACTGG 3 cut(s) 192, 430, 450
BssMI GATC 3 cut(s) 73, 278, 463
BssNI GRCGYC 1 cut(s) 213
Bst4CI ACNGT 2 cut(s) 367, 485
Bst6I CTCTTC 1 cut(s) 572
BstACI GRCGYC 1 cut(s) 213
BstC8I GCNNGC 1 cut(s) 508
BstDEI CTNAG 2 cut(s) 375, 412
BstF5I GGATG 1 cut(s) 66
BstFNI CGCG 3 cut(s) 103, 469, 471
BstHHI GCGC 2 cut(s) 103, 471
BstKTI GATC 3 cut(s) 76, 281, 466
BstMBI GATC 3 cut(s) 73, 278, 463
BstMWI GCNNNNNNNGC 3 cut(s) 13, 22, 107
BstNSI RCATGY 1 cut(s) 510
BstSFI CTRYAG 2 cut(s) 291, 501
BstUI CGCG 3 cut(s) 103, 469, 471
BstV1I GCAGC 1 cut(s) 515
BstX2I RGATCY 1 cut(s) 278
BstXI CCANNNNNNTGG 1 cut(s) 54
BstYI RGATCY 1 cut(s) 278
BsuRI GGCC 1 cut(s) 46
BtsCI GGATG 1 cut(s) 66
BtsIMutI CAGTG 2 cut(s) 199, 372
Cac8I GCNNGC 1 cut(s) 508
CfoI GCGC 2 cut(s) 103, 471
Cfr13I GGNCC 1 cut(s) 44
CseI GACGC 1 cut(s) 221
CviAII CATG 3 cut(s) 260, 507, 601
CviJI RGCY 8 cut(s) 7, 46, 110, 246, 350, 416, 461, 523
CviKI_1 RGCY 8 cut(s) 7, 46, 110, 246, 350, 416, 461, 523
DdeI CTNAG 2 cut(s) 375, 412
DpnI GATC 3 cut(s) 75, 280, 465
DpnII GATC 3 cut(s) 73, 278, 463
Eam1104I CTCTTC 1 cut(s) 572
EarI CTCTTC 1 cut(s) 572
EciI GGCGGA 1 cut(s) 441
Eco57I CTGAAG 1 cut(s) 441
Eco88I CYCGRG 1 cut(s) 345
EcoRI GAATTC 3 cut(s) 332, 407, 539
FaeI CATG 3 cut(s) 263, 510, 604
FaiI YATR 8 cut(s) 261, 370, 402, 420, 443, 508, 554, 602
FatI CATG 3 cut(s) 259, 506, 600
Fnu4HI GCNGC 1 cut(s) 504
FokI GGATG 1 cut(s) 53
Fsp4HI GCNGC 1 cut(s) 504
FspBI CTAG 1 cut(s) 92
GlaI GCGC 2 cut(s) 102, 470
GluI GCNGC 1 cut(s) 504
GsuI CTGGAG 2 cut(s) 38, 333
HaeIII GGCC 1 cut(s) 46
HapII CCGG 1 cut(s) 216
HgaI GACGC 1 cut(s) 221
HhaI GCGC 2 cut(s) 103, 471
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 3 cut(s) 263, 510, 604
Hin6I GCGC 2 cut(s) 101, 469
HinP1I GCGC 2 cut(s) 101, 469
HindIII AAGCTT 1 cut(s) 244
HinfI GANTC 5 cut(s) 219, 287, 308, 379, 496
HpaII CCGG 1 cut(s) 216
HphI GGTGA 2 cut(s) 146, 160
Hpy166II GTNNAC 1 cut(s) 427
Hpy188III TCNNGA 6 cut(s) 227, 272, 312, 345, 515, 574
Hpy8I GTNNAC 1 cut(s) 427
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 1 cut(s) 465
HpyCH4III ACNGT 2 cut(s) 367, 485
HpyCH4IV ACGT 1 cut(s) 423
HpyCH4V TGCA 8 cut(s) 25, 154, 325, 372, 489, 503, 510, 535
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 22, 107
HpyF3I CTNAG 2 cut(s) 375, 412
HpySE526I ACGT 1 cut(s) 423
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 3 cut(s) 263, 510, 604
HspAI GCGC 2 cut(s) 101, 469
Kzo9I GATC 3 cut(s) 73, 278, 463
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 1 cut(s) 515
LweI GCATC 2 cut(s) 12, 152
MaeI CTAG 1 cut(s) 92
MaeII ACGT 1 cut(s) 423
MaeIII GTNAC 2 cut(s) 158, 284
MalI GATC 3 cut(s) 75, 280, 465
MboI GATC 3 cut(s) 73, 278, 463
MboII GAAGA 5 cut(s) 68, 317, 350, 389, 589
MflI RGATCY 1 cut(s) 278
MluCI AATT 5 cut(s) 332, 407, 491, 539, 596
MlyI GAGTC 1 cut(s) 281
MnlI CCTC 5 cut(s) 35, 308, 573, 576, 603
MseI TTAA 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 461
MspI CCGG 1 cut(s) 216
MvnI CGCG 3 cut(s) 103, 469, 471
MwoI GCNNNNNNNGC 3 cut(s) 13, 22, 107
NdeII GATC 3 cut(s) 73, 278, 463
NlaIII CATG 3 cut(s) 263, 510, 604
NlaIV GGNNCC 3 cut(s) 252, 450, 482
NmuCI GTSAC 1 cut(s) 284
NspI RCATGY 1 cut(s) 510
PaeI GCATGC 1 cut(s) 510
PaeR7I CTCGAG 1 cut(s) 345
PfeI GAWTC 4 cut(s) 219, 308, 379, 496
PkrI GCNGC 1 cut(s) 505
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PspN4I GGNNCC 3 cut(s) 252, 450, 482
PspPI GGNCC 1 cut(s) 44
PstI CTGCAG 1 cut(s) 505
PsuI RGATCY 1 cut(s) 278
PvuII CAGCTG 1 cut(s) 461
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 1 cut(s) 504
Sau3AI GATC 3 cut(s) 73, 278, 463
Sau96I GGNCC 1 cut(s) 44
SchI GAGTC 1 cut(s) 281
SetI ASST 8 cut(s) 9, 91, 124, 248, 352, 426, 463, 525
SfaNI GCATC 2 cut(s) 12, 152
SfcI CTRYAG 2 cut(s) 291, 501
Sfr274I CTCGAG 1 cut(s) 345
SlaI CTCGAG 1 cut(s) 345
SmlI CTYRAG 2 cut(s) 205, 345
SmoI CTYRAG 2 cut(s) 205, 345
SphI GCATGC 1 cut(s) 510
Sse9I AATT 5 cut(s) 332, 407, 491, 539, 596
SsiI CCGC 3 cut(s) 452, 467, 471
SspMI CTAG 1 cut(s) 92
TaaI ACNGT 2 cut(s) 367, 485
TaiI ACGT 1 cut(s) 426
TaqI TCGA 2 cut(s) 228, 346
TasI AATT 5 cut(s) 332, 407, 491, 539, 596
TfiI GAWTC 4 cut(s) 219, 308, 379, 496
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TscAI CASTG 2 cut(s) 199, 379
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 1 cut(s) 503
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 4 cut(s) 246, 371, 389, 589
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 2 cut(s) 199, 379
XapI RAATTY 3 cut(s) 332, 407, 539
XceI RCATGY 1 cut(s) 510
XhoI CTCGAG 1 cut(s) 345
XspI CTAG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.