pycom01g21800

Ferric reduction oxidase 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
N/A
Physical Location & Seq
Reverse (-)
19670716 .. 19672117
1402 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 690 bp
ATGGAAGGGTTGCTATGTAATATACAGGCAAGCATACATATATTGCAGCCACTCACATACGTTTGTATAGTACAAGAACTACCAGTACTACTGGGGAGATTGCAATTGTGTTGGCAATGGCCATGTGGATCACAAGCATTCCTCGCGTTAGGCGTAAAATGTTTGAACTTTTCTTCTACACCCACCATCTCTACACACTCTACATCTTCTTCTACGTACTGCATGTCGCTGCTTCCTACTTCTTCTCAGCTCGCATTTTACCATGTGGTACCATTGAGCTCAACTTCGCCAAGACCCAAGGTAACTAATTTTGCTCTAGTCGTCATTATTAAATACGTTGAGCTCATGGCACATGTTGGAAAATATCAAATTTCGTTGTCATTAAGATTGAAGGAAGTTGGTCTCAAAAACTCTACAAACAACTGTCTTCAAATGTGGATCGATATCCAAGCTGCTGTCGAAGGACCTTATGGCCCCATTTCATCTCATTTTCTAAGGCACGAGGCTTTGGTGATGCTCAGTGGAGGCAGTGGAATCACTCCTCTCATTTCCATAATCCGTGAAATTATTTTCCAAACCACCAAACCCGATTCCCATTTGCCACGTGTACGGCTCATATGTGCCTTCAAGAACACAGATCGATCTCTCCATGCTACAACTCTTGCTTCCTATTTCTTCAACCCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000293 GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0016723 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_8 Ferric_reduct NAD_binding_6

Protein Analysis

230

Amino Acids

25.37

Weight (kDa)

8.95

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_6 PF08030 167 - 213 3.3e-07 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 268
AccB1I GGYRCC 1 cut(s) 268
AccII CGCG 1 cut(s) 146
AclWI GGATC 2 cut(s) 136, 446
AcoI YGGCCR 1 cut(s) 119
AcsI RAATTY 1 cut(s) 369
AcvI CACGTG 1 cut(s) 605
AfaI GTAC 5 cut(s) 72, 87, 218, 270, 609
AflIII ACRYGT 2 cut(s) 352, 604
AgsI TTSAA 5 cut(s) 166, 391, 431, 628, 679
AluBI AGCT 4 cut(s) 250, 279, 343, 452
AluI AGCT 4 cut(s) 250, 279, 343, 452
Alw21I GWGCWC 2 cut(s) 281, 345
Alw26I GTCTC 1 cut(s) 407
AlwI GGATC 2 cut(s) 136, 446
AoxI GGCC 2 cut(s) 119, 472
ApeKI GCWGC 3 cut(s) 46, 229, 452
ApoI RAATTY 1 cut(s) 369
Asp718I GGTACC 1 cut(s) 268
AspS9I GGNCC 2 cut(s) 464, 473
AsuHPI GGTGA 1 cut(s) 523
AvaII GGWCC 1 cut(s) 464
BalI TGGCCA 1 cut(s) 121
BanI GGYRCC 1 cut(s) 268
BanII GRGCYC 2 cut(s) 281, 345
BauI CACGAG 1 cut(s) 500
BbrPI CACGTG 1 cut(s) 605
BbsI GAAGAC 1 cut(s) 419
Bbv12I GWGCWC 2 cut(s) 281, 345
BbvI GCAGC 3 cut(s) 58, 216, 439
BccI CCATC 1 cut(s) 194
BceAI ACGGC 1 cut(s) 626
BcoDI GTCTC 1 cut(s) 407
BfaI CTAG 1 cut(s) 317
BisI GCNGC 3 cut(s) 47, 230, 453
BlsI GCNGC 3 cut(s) 48, 231, 454
BmcAI AGTACT 1 cut(s) 87
Bme18I GGWCC 1 cut(s) 464
BmgT120I GGNCC 2 cut(s) 464, 473
BmiI GGNNCC 2 cut(s) 270, 475
BmrI ACTGGG 1 cut(s) 101
BmsI GCATC 1 cut(s) 504
BmuI ACTGGG 1 cut(s) 101
BpiI GAAGAC 1 cut(s) 419
Bsa29I ATCGAT 2 cut(s) 441, 640
BsaAI YACGTR 2 cut(s) 216, 605
BsaBI GATNNNNATC 1 cut(s) 443
BsaI GGTCTC 1 cut(s) 407
BsaJI CCNNGG 1 cut(s) 297
Bse1I ACTGG 2 cut(s) 83, 96
Bse3DI GCAATG 1 cut(s) 122
Bse8I GATNNNNATC 1 cut(s) 443
BseCI ATCGAT 2 cut(s) 441, 640
BseDI CCNNGG 1 cut(s) 297
BseJI GATNNNNATC 1 cut(s) 443
BseMI GCAATG 1 cut(s) 122
BseMII CTCAG 2 cut(s) 260, 532
BseNI ACTGG 2 cut(s) 83, 96
BseRI GAGGAG 1 cut(s) 531
BseXI GCAGC 3 cut(s) 58, 216, 439
Bsh1236I CGCG 1 cut(s) 146
BshFI GGCC 2 cut(s) 121, 474
BshNI GGYRCC 1 cut(s) 268
BshVI ATCGAT 2 cut(s) 441, 640
BsiHKAI GWGCWC 2 cut(s) 281, 345
BsmAI GTCTC 1 cut(s) 407
BsmI GAATGC 1 cut(s) 137
BsnI GGCC 2 cut(s) 121, 474
Bso31I GGTCTC 1 cut(s) 407
Bsp1286I GDGCHC 2 cut(s) 281, 345
Bsp143I GATC 4 cut(s) 128, 438, 637, 641
BspANI GGCC 2 cut(s) 121, 474
BspCNI CTCAG 2 cut(s) 259, 531
BspDI ATCGAT 2 cut(s) 441, 640
BspFNI CGCG 1 cut(s) 146
BspLI GGNNCC 2 cut(s) 270, 475
BspPI GGATC 2 cut(s) 136, 446
BspT107I GGYRCC 1 cut(s) 268
BspTNI GGTCTC 1 cut(s) 407
BsrDI GCAATG 1 cut(s) 122
BsrI ACTGG 2 cut(s) 83, 96
BssECI CCNNGG 1 cut(s) 297
BssMI GATC 4 cut(s) 128, 438, 637, 641
BssSI CACGAG 1 cut(s) 500
BssT1I CCWWGG 1 cut(s) 297
Bst2BI CACGAG 1 cut(s) 500
Bst4CI ACNGT 1 cut(s) 425
BstBAI YACGTR 2 cut(s) 216, 605
BstC8I GCNNGC 2 cut(s) 31, 252
BstDEI CTNAG 3 cut(s) 246, 494, 518
BstFNI CGCG 1 cut(s) 146
BstKTI GATC 4 cut(s) 131, 441, 640, 644
BstMAI GTCTC 1 cut(s) 407
BstMBI GATC 4 cut(s) 128, 438, 637, 641
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstNSI RCATGY 2 cut(s) 226, 356
BstSNI TACGTA 1 cut(s) 216
BstUI CGCG 1 cut(s) 146
BstV1I GCAGC 3 cut(s) 58, 216, 439
BstV2I GAAGAC 1 cut(s) 419
Bsu15I ATCGAT 2 cut(s) 441, 640
BsuRI GGCC 2 cut(s) 121, 474
BsuTUI ATCGAT 2 cut(s) 441, 640
BtsI GCAGTG 1 cut(s) 535
BtsIMutI CAGTG 2 cut(s) 526, 535
Cac8I GCNNGC 2 cut(s) 31, 252
Cfr13I GGNCC 2 cut(s) 464, 473
ClaI ATCGAT 2 cut(s) 441, 640
Csp6I GTAC 5 cut(s) 71, 86, 217, 269, 608
CviAII CATG 6 cut(s) 123, 223, 263, 346, 353, 650
CviJI RGCY 9 cut(s) 49, 121, 250, 279, 343, 452, 474, 506, 613
CviKI_1 RGCY 9 cut(s) 49, 121, 250, 279, 343, 452, 474, 506, 613
CviQI GTAC 5 cut(s) 71, 86, 217, 269, 608
DdeI CTNAG 3 cut(s) 246, 494, 518
DpnI GATC 4 cut(s) 130, 440, 639, 643
DpnII GATC 4 cut(s) 128, 438, 637, 641
EaeI YGGCCR 1 cut(s) 119
Ecl136II GAGCTC 2 cut(s) 279, 343
Eco105I TACGTA 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 297
Eco24I GRGCYC 2 cut(s) 281, 345
Eco31I GGTCTC 1 cut(s) 407
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 1 cut(s) 464
Eco53kI GAGCTC 2 cut(s) 279, 343
Eco72I CACGTG 1 cut(s) 605
EcoICRI GAGCTC 2 cut(s) 279, 343
EcoO109I RGGNCCY 1 cut(s) 464
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 297
EcoT38I GRGCYC 2 cut(s) 281, 345
ErhI CCWWGG 1 cut(s) 297
FaeI CATG 6 cut(s) 126, 226, 266, 349, 356, 653
FatI CATG 6 cut(s) 122, 222, 262, 345, 352, 649
FauNDI CATATG 1 cut(s) 617
Fnu4HI GCNGC 3 cut(s) 47, 230, 453
FriOI GRGCYC 2 cut(s) 281, 345
Fsp4HI GCNGC 3 cut(s) 47, 230, 453
FspBI CTAG 1 cut(s) 317
GluI GCNGC 3 cut(s) 47, 230, 453
HaeIII GGCC 2 cut(s) 121, 474
Hin1II CATG 6 cut(s) 126, 226, 266, 349, 356, 653
HinfI GANTC 2 cut(s) 534, 590
HphI GGTGA 1 cut(s) 523
Hpy166II GTNNAC 1 cut(s) 608
Hpy188III TCNNGA 1 cut(s) 628
Hpy8I GTNNAC 1 cut(s) 608
HpyAV CCTTC 3 cut(s) 385, 455, 634
HpyCH4III ACNGT 1 cut(s) 425
HpyCH4IV ACGT 4 cut(s) 60, 215, 336, 604
HpyCH4V TGCA 3 cut(s) 46, 103, 222
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 3 cut(s) 246, 494, 518
HpySE526I ACGT 4 cut(s) 60, 215, 336, 604
Hsp92II CATG 6 cut(s) 126, 226, 266, 349, 356, 653
KpnI GGTACC 1 cut(s) 272
Kzo9I GATC 4 cut(s) 128, 438, 637, 641
LpnPI CCDG 3 cut(s) 11, 77, 96
Lsp1109I GCAGC 3 cut(s) 58, 216, 439
LweI GCATC 1 cut(s) 504
MaeI CTAG 1 cut(s) 317
MaeII ACGT 4 cut(s) 60, 215, 336, 604
MaeIII GTNAC 1 cut(s) 301
MalI GATC 4 cut(s) 130, 440, 639, 643
MboI GATC 4 cut(s) 128, 438, 637, 641
MboII GAAGA 6 cut(s) 165, 198, 201, 234, 419, 667
MfeI CAATTG 1 cut(s) 104
MhlI GDGCHC 2 cut(s) 281, 345
MlsI TGGCCA 1 cut(s) 121
MluCI AATT 4 cut(s) 104, 307, 369, 564
MluNI TGGCCA 1 cut(s) 121
MmeI TCCRAC 1 cut(s) 337
MnlI CCTC 4 cut(s) 152, 496, 518, 552
Mox20I TGGCCA 1 cut(s) 121
MscI TGGCCA 1 cut(s) 121
MseI TTAA 2 cut(s) 330, 383
Msp20I TGGCCA 1 cut(s) 121
MunI CAATTG 1 cut(s) 104
Mva1269I GAATGC 1 cut(s) 137
MvnI CGCG 1 cut(s) 146
MwoI GCNNNNNNNGC 1 cut(s) 143
NdeI CATATG 1 cut(s) 617
NdeII GATC 4 cut(s) 128, 438, 637, 641
NlaIII CATG 6 cut(s) 126, 226, 266, 349, 356, 653
NlaIV GGNNCC 2 cut(s) 270, 475
NspI RCATGY 2 cut(s) 226, 356
PciI ACATGT 1 cut(s) 352
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PctI GAATGC 1 cut(s) 137
PfeI GAWTC 2 cut(s) 534, 590
PkrI GCNGC 3 cut(s) 48, 231, 454
PmaCI CACGTG 1 cut(s) 605
PmlI CACGTG 1 cut(s) 605
Ppu21I YACGTR 2 cut(s) 216, 605
PpuMI RGGWCCY 1 cut(s) 464
PscI ACATGT 1 cut(s) 352
Psp124BI GAGCTC 2 cut(s) 281, 345
Psp5II RGGWCCY 1 cut(s) 464
PspCI CACGTG 1 cut(s) 605
PspN4I GGNNCC 2 cut(s) 270, 475
PspPI GGNCC 2 cut(s) 464, 473
PspPPI RGGWCCY 1 cut(s) 464
PsrI GAACNNNNNNTAC 2 cut(s) 69, 101
RsaI GTAC 5 cut(s) 72, 87, 218, 270, 609
RsaNI GTAC 5 cut(s) 71, 86, 217, 269, 608
SacI GAGCTC 2 cut(s) 281, 345
SaqAI TTAA 2 cut(s) 330, 383
SatI GCNGC 3 cut(s) 47, 230, 453
Sau3AI GATC 4 cut(s) 128, 438, 637, 641
Sau96I GGNCC 2 cut(s) 464, 473
ScaI AGTACT 1 cut(s) 87
SduI GDGCHC 2 cut(s) 281, 345
SfaNI GCATC 1 cut(s) 504
SinI GGWCC 1 cut(s) 464
SnaBI TACGTA 1 cut(s) 216
Sse9I AATT 4 cut(s) 104, 307, 369, 564
SspMI CTAG 1 cut(s) 317
SstI GAGCTC 2 cut(s) 281, 345
StyI CCWWGG 1 cut(s) 297
TaaI ACNGT 1 cut(s) 425
TaiI ACGT 4 cut(s) 63, 218, 339, 607
TaqI TCGA 3 cut(s) 441, 459, 640
TasI AATT 4 cut(s) 104, 307, 369, 564
TatI WGTACW 2 cut(s) 70, 85
TfiI GAWTC 2 cut(s) 534, 590
Tru1I TTAA 2 cut(s) 330, 383
Tru9I TTAA 2 cut(s) 330, 383
TscAI CASTG 2 cut(s) 526, 535
TseI GCWGC 3 cut(s) 46, 229, 452
TspDTI ATGAA 1 cut(s) 471
TspGWI ACGGA 1 cut(s) 548
TspRI CASTG 2 cut(s) 526, 535
VpaK11BI GGWCC 1 cut(s) 464
XapI RAATTY 1 cut(s) 369
XceI RCATGY 2 cut(s) 226, 356
XspI CTAG 1 cut(s) 317
ZrmI AGTACT 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.