Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

N/A

Physical Location & Seq

Genomic Coordinates Reverse (-)

624919 .. 626479

Gene Structure 1561 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 984 bp

ATGGGCCGGCTTGTCTGTCCCGGCCCAATTCACAAGTCCATTATTAACCAAGGAGTCACGTGCTACTGCCAGATATTATCAACCTCTTCCTCTTTCCCGCCAAATCATTCCCGCCAAAACGGCGCGCTCCTCTTCCGCGCGGTAGAGAGAGAGAGCAGAGACATGTCAGGGTGGGACGAAGGAGCGATCTACTACAGCGACCAAGCGCAGTCGCTTGGCGGCGACGGCGGAGACGCCGGAAGCAACGCCGCAGCCGCCACCCGCCACTCCGTTCTTCAGAAATTCAAGGAATTCATCCGTAGCTTCGAGACGCAGAAAAATGTGTTTTCGTACAGGGAGAGCCTCCTCCACAACGCTAAGTACCTTCTCGTCGACATCGAAGACCTCGACTCCTTCGACGCTGACCTCGTCGCAAAGCTCCGCTCTGCCCCATCCGATTACTTGCCTCTGTTTGAAAACGCCGCCGGTCAGGTTTTGGCGAATTTAAAGACGAAAGTGCCCGGAAATGAAGGGAAATTGGAGGAGCGGGTGCCGGAGGATGTTCAGATCTTGCTGACGTCCAAAGAAGACCCGGCGTCTATGCGCCAGCTTGGGGCACAATGTATATCGAAACTCATCAAGGTTTCTGGGATCACTATAGCTGCTTCGAGGGTCAAGGCGAAGGCAACTTATGTGATTGTCATGTGTAAGAACTGTAAAAATGTGCAAAGGGTACCTTGCCGGCCAGGCCTTGGTGGCGCAATTGTCCCTCGCTCTTGCAACCATATTCCTCAGCCTGGAGAAGAACCTTGTCCGCTTGATCGATCGTCGGTTGTGGTTCCTGATAAGAGCAAGTATGTTGATCAGCAAACCTTGAAATTGCAAGAGAATCCAGAGGATGTACCCACCGGAGAGCTTCCAAGAAATATGCTTCTATCCGTGGATCGATCATCTCGTTCAGACAATTGTGCCTGGCACAAGATTAACCATCATGGGCATTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000347 GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 ko05206 map03030 map04110 map04111 map04113 map05206

Pfam Domains

Protein Families

MCM MCM_N MCM_OB Mg_chelatase

Protein Analysis

328

Amino Acids

35.91

Weight (kDa)

8.0

Isoelectric Point (pI)

47.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_N	PF14551	93 - 182	4e-14	MCM N-terminal domain
MCM_OB	PF17207	194 - 310	5.8e-26	MCM OB domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	560
Acc65I	GGTACC	1 cut(s)	712
AccB1I	GGYRCC	2 cut(s)	529, 712
AccB7I	CCANNNNNTGG	1 cut(s)	731
AccBSI	CCGCTC	2 cut(s)	423, 526
AccI	GTMKAC	1 cut(s)	372
AccII	CGCG	3 cut(s)	125, 138, 140
AclWI	GGATC	2 cut(s)	638, 930
AcoI	YGGCCR	1 cut(s)	722
AcsI	RAATTY	3 cut(s)	281, 290, 481
AcuI	CTGAAG	1 cut(s)	260
AcvI	CACGTG	1 cut(s)	60
AcyI	GRCGYC	3 cut(s)	234, 557, 575
AfaI	GTAC	4 cut(s)	332, 362, 714, 882
AfiI	CCNNNNNNNGG	3 cut(s)	592, 731, 776
AflIII	ACRYGT	1 cut(s)	162
AgsI	TTSAA	3 cut(s)	286, 455, 856
AhdI	GACNNNNNGTC	1 cut(s)	574
AjnI	CCWGG	3 cut(s)	724, 775, 950
AluBI	AGCT	5 cut(s)	303, 418, 589, 641, 895
AluI	AGCT	5 cut(s)	303, 418, 589, 641, 895
Alw26I	GTCTC	3 cut(s)	153, 225, 302
AlwI	GGATC	2 cut(s)	638, 930
AoxI	GGCC	4 cut(s)	4, 22, 722, 727
ApeKI	GCWGC	2 cut(s)	251, 641
ApoI	RAATTY	3 cut(s)	281, 290, 481
Asp718I	GGTACC	1 cut(s)	712
AspLEI	GCGC	6 cut(s)	125, 127, 140, 208, 585, 740
AspS9I	GGNCC	2 cut(s)	4, 23
AsuC2I	CCSGG	3 cut(s)	21, 501, 572
BaeGI	GKGCMC	2 cut(s)	501, 598
BanI	GGYRCC	2 cut(s)	529, 712
BbrPI	CACGTG	1 cut(s)	60
BbsI	GAAGAC	2 cut(s)	387, 573
BbvCI	CCTCAGC	1 cut(s)	771
BbvI	GCAGC	2 cut(s)	263, 628
BccI	CCATC	2 cut(s)	439, 975
BceAI	ACGGC	2 cut(s)	136, 241
BciT130I	CCWGG	3 cut(s)	726, 777, 952
BclI	TGATCA	1 cut(s)	841
BcnI	CCSGG	3 cut(s)	21, 501, 572
BcoDI	GTCTC	3 cut(s)	153, 225, 302
BfmI	CTRYAG	2 cut(s)	193, 636
BglI	GCCNNNNNGGC	3 cut(s)	120, 726, 735
BglII	AGATCT	1 cut(s)	546
BisI	GCNGC	6 cut(s)	220, 249, 252, 255, 462, 642
BlsI	GCNGC	6 cut(s)	221, 250, 253, 256, 463, 643
Bme1390I	CCNGG	6 cut(s)	21, 501, 572, 726, 777, 952
BmeRI	GACNNNNNGTC	1 cut(s)	574
BmgT120I	GGNCC	2 cut(s)	4, 23
BmiI	GGNNCC	3 cut(s)	531, 714, 819
BmrFI	CCNGG	6 cut(s)	21, 501, 572, 726, 777, 952
BpiI	GAAGAC	2 cut(s)	387, 573
BpmI	CTGGAG	1 cut(s)	798
Bpu10I	CCTNAGC	1 cut(s)	771
BpuMI	CCSGG	3 cut(s)	21, 501, 572
Bsa29I	ATCGAT	2 cut(s)	802, 925
BsaAI	YACGTR	1 cut(s)	60
BsaHI	GRCGYC	3 cut(s)	234, 557, 575
BsaJI	CCNNGG	3 cut(s)	49, 730, 918
BsaWI	WCCGGW	1 cut(s)	887
BsaXI	ACNNNNNCTCC	2 cut(s)	374, 404
Bsc4I	CCNNNNNNNGG	3 cut(s)	592, 731, 776
Bse118I	RCCGGY	3 cut(s)	6, 464, 720
BseBI	CCWGG	3 cut(s)	726, 777, 952
BseCI	ATCGAT	2 cut(s)	802, 925
BseDI	CCNNGG	3 cut(s)	49, 730, 918
BseGI	GGATG	4 cut(s)	294, 431, 544, 883
BseLI	CCNNNNNNNGG	3 cut(s)	592, 731, 776
BseMII	CTCAG	1 cut(s)	785
BsePI	GCGCGC	1 cut(s)	123
BseRI	GAGGAG	3 cut(s)	119, 335, 536
BseSI	GKGCMC	2 cut(s)	501, 598
BseXI	GCAGC	2 cut(s)	263, 628
Bsh1236I	CGCG	3 cut(s)	125, 138, 140
Bsh1285I	CGRYCG	1 cut(s)	806
BshFI	GGCC	4 cut(s)	6, 24, 724, 729
BshNI	GGYRCC	2 cut(s)	529, 712
BshVI	ATCGAT	2 cut(s)	802, 925
BsiEI	CGRYCG	1 cut(s)	806
BsiSI	CCGG	9 cut(s)	7, 21, 237, 465, 501, 533, 572, 721, 888
BslFI	GGGAC	3 cut(s)	3, 188, 731
BslI	CCNNNNNNNGG	3 cut(s)	592, 731, 776
BsmAI	GTCTC	3 cut(s)	153, 225, 302
BsmBI	CGTCTC	2 cut(s)	225, 302
BsmFI	GGGAC	3 cut(s)	3, 188, 731
BsnI	GGCC	4 cut(s)	6, 24, 724, 729
Bsp1286I	GDGCHC	2 cut(s)	501, 598
Bsp143I	GATC	8 cut(s)	186, 546, 630, 799, 803, 841, 922, 926
BspANI	GGCC	4 cut(s)	6, 24, 724, 729
BspCNI	CTCAG	1 cut(s)	784
BspDI	ATCGAT	2 cut(s)	802, 925
BspFNI	CGCG	3 cut(s)	125, 138, 140
BspLI	GGNNCC	3 cut(s)	531, 714, 819
BspPI	GGATC	2 cut(s)	638, 930
BspT107I	GGYRCC	2 cut(s)	529, 712
BsrBI	CCGCTC	2 cut(s)	423, 526
BsrFI	RCCGGY	3 cut(s)	6, 464, 720
BssAI	RCCGGY	3 cut(s)	6, 464, 720
BssECI	CCNNGG	3 cut(s)	49, 730, 918
BssHII	GCGCGC	1 cut(s)	123
BssMI	GATC	8 cut(s)	186, 546, 630, 799, 803, 841, 922, 926
BssNI	GRCGYC	3 cut(s)	234, 557, 575
BssT1I	CCWWGG	2 cut(s)	49, 730
Bst2UI	CCWGG	3 cut(s)	726, 777, 952
Bst4CI	ACNGT	1 cut(s)	695
Bst6I	CTCTTC	2 cut(s)	91, 137
BstACI	GRCGYC	3 cut(s)	234, 557, 575
BstBAI	YACGTR	1 cut(s)	60
BstC8I	GCNNGC	4 cut(s)	8, 125, 587, 722
BstDEI	CTNAG	2 cut(s)	357, 771
BstDSI	CCRYGG	1 cut(s)	918
BstF5I	GGATG	4 cut(s)	294, 431, 544, 883
BstFNI	CGCG	3 cut(s)	125, 138, 140
BstHHI	GCGC	6 cut(s)	125, 127, 140, 208, 585, 740
BstKTI	GATC	8 cut(s)	189, 549, 633, 802, 806, 844, 925, 929
BstMAI	GTCTC	3 cut(s)	153, 225, 302
BstMBI	GATC	8 cut(s)	186, 546, 630, 799, 803, 841, 922, 926
BstMCI	CGRYCG	1 cut(s)	806
BstMWI	GCNNNNNNNGC	5 cut(s)	120, 225, 254, 726, 735
BstNI	CCWGG	3 cut(s)	726, 777, 952
BstNSI	RCATGY	1 cut(s)	166
BstSCI	CCNGG	6 cut(s)	19, 499, 570, 724, 775, 950
BstSFI	CTRYAG	2 cut(s)	193, 636
BstSLI	GKGCMC	2 cut(s)	501, 598
BstUI	CGCG	3 cut(s)	125, 138, 140
BstV1I	GCAGC	2 cut(s)	263, 628
BstV2I	GAAGAC	2 cut(s)	387, 573
BstX2I	RGATCY	1 cut(s)	546
BstYI	RGATCY	1 cut(s)	546
Bsu15I	ATCGAT	2 cut(s)	802, 925
BsuRI	GGCC	4 cut(s)	6, 24, 724, 729
BsuTUI	ATCGAT	2 cut(s)	802, 925
BtgI	CCRYGG	1 cut(s)	918
BtsCI	GGATG	4 cut(s)	294, 431, 544, 883
Cac8I	GCNNGC	4 cut(s)	8, 125, 587, 722
CfoI	GCGC	6 cut(s)	125, 127, 140, 208, 585, 740
Cfr10I	RCCGGY	3 cut(s)	6, 464, 720
Cfr13I	GGNCC	2 cut(s)	4, 23
ClaI	ATCGAT	2 cut(s)	802, 925
CseI	GACGC	4 cut(s)	242, 319, 407, 564
Csp6I	GTAC	4 cut(s)	331, 361, 713, 881
CviAII	CATG	3 cut(s)	163, 682, 971
CviQI	GTAC	4 cut(s)	331, 361, 713, 881
DdeI	CTNAG	2 cut(s)	357, 771
DpnI	GATC	8 cut(s)	188, 548, 632, 801, 805, 843, 924, 928
DpnII	GATC	8 cut(s)	186, 546, 630, 799, 803, 841, 922, 926
DraI	TTTAAA	1 cut(s)	486
DriI	GACNNNNNGTC	1 cut(s)	574
EaeI	YGGCCR	1 cut(s)	722
Eam1104I	CTCTTC	2 cut(s)	91, 137
Eam1105I	GACNNNNNGTC	1 cut(s)	574
EarI	CTCTTC	2 cut(s)	91, 137
EciI	GGCGGA	1 cut(s)	243
Eco130I	CCWWGG	2 cut(s)	49, 730
Eco147I	AGGCCT	1 cut(s)	729
Eco57I	CTGAAG	1 cut(s)	260
Eco72I	CACGTG	1 cut(s)	60
EcoRI	GAATTC	1 cut(s)	290
EcoRII	CCWGG	3 cut(s)	724, 775, 950
EcoT14I	CCWWGG	2 cut(s)	49, 730
ErhI	CCWWGG	2 cut(s)	49, 730
Esp3I	CGTCTC	2 cut(s)	225, 302
FaeI	CATG	3 cut(s)	166, 685, 974
FalI	AAGNNNNNCTT	2 cut(s)	700, 732
FaqI	GGGAC	3 cut(s)	3, 188, 731
FatI	CATG	3 cut(s)	162, 681, 970
FauI	CCCGC	4 cut(s)	105, 119, 269, 519
FbaI	TGATCA	1 cut(s)	841
FblI	GTMKAC	1 cut(s)	372
Fnu4HI	GCNGC	6 cut(s)	220, 249, 252, 255, 462, 642
FokI	GGATG	4 cut(s)	281, 418, 551, 890
Fsp4HI	GCNGC	6 cut(s)	220, 249, 252, 255, 462, 642
GlaI	GCGC	6 cut(s)	124, 126, 139, 207, 584, 739
GluI	GCNGC	6 cut(s)	220, 249, 252, 255, 462, 642
GsuI	CTGGAG	1 cut(s)	798
HaeIII	GGCC	4 cut(s)	6, 24, 724, 729
HapII	CCGG	9 cut(s)	7, 21, 237, 465, 501, 533, 572, 721, 888
HgaI	GACGC	4 cut(s)	242, 319, 407, 564
HhaI	GCGC	6 cut(s)	125, 127, 140, 208, 585, 740
Hin1I	GRCGYC	3 cut(s)	234, 557, 575
Hin1II	CATG	3 cut(s)	166, 685, 974
Hin6I	GCGC	6 cut(s)	123, 125, 138, 206, 583, 738
HinP1I	GCGC	6 cut(s)	123, 125, 138, 206, 583, 738
HincII	GTYRAC	1 cut(s)	373
HindII	GTYRAC	1 cut(s)	373
HinfI	GANTC	3 cut(s)	54, 389, 868
HpaII	CCGG	9 cut(s)	7, 21, 237, 465, 501, 533, 572, 721, 888
Hpy166II	GTNNAC	1 cut(s)	373
Hpy188I	TCNGA	4 cut(s)	279, 436, 546, 940
Hpy188III	TCNNGA	3 cut(s)	307, 821, 872
Hpy8I	GTNNAC	1 cut(s)	373
Hpy99I	CGWCG	5 cut(s)	227, 374, 401, 413, 811
HpyAV	CCTTC	5 cut(s)	173, 374, 403, 503, 655
HpyCH4III	ACNGT	1 cut(s)	695
HpyCH4IV	ACGT	2 cut(s)	59, 557
HpyCH4V	TGCA	3 cut(s)	706, 759, 862
HpyF10VI	GCNNNNNNNGC	5 cut(s)	120, 225, 254, 726, 735
HpyF3I	CTNAG	2 cut(s)	357, 771
HpySE526I	ACGT	2 cut(s)	59, 557
Hsp92I	GRCGYC	3 cut(s)	234, 557, 575
Hsp92II	CATG	3 cut(s)	166, 685, 974
HspAI	GCGC	6 cut(s)	123, 125, 138, 206, 583, 738
KpnI	GGTACC	1 cut(s)	716
KroI	GCCGGC	2 cut(s)	6, 720
KroNI	GCCGGC	2 cut(s)	8, 722
Ksp22I	TGATCA	1 cut(s)	841
Kzo9I	GATC	8 cut(s)	186, 546, 630, 799, 803, 841, 922, 926
LmnI	GCTCC	4 cut(s)	132, 182, 423, 523
Lsp1109I	GCAGC	2 cut(s)	263, 628
MaeII	ACGT	2 cut(s)	59, 557
MaeIII	GTNAC	1 cut(s)	55
MalI	GATC	8 cut(s)	188, 548, 632, 801, 805, 843, 924, 928
MbiI	CCGCTC	2 cut(s)	423, 526
MboI	GATC	8 cut(s)	186, 546, 630, 799, 803, 841, 922, 926
MboII	GAAGA	6 cut(s)	78, 124, 266, 392, 578, 794
MfeI	CAATTG	2 cut(s)	741, 943
MflI	RGATCY	1 cut(s)	546
MhlI	GDGCHC	2 cut(s)	501, 598
MluCI	AATT	8 cut(s)	27, 281, 290, 481, 515, 741, 857, 943
MlyI	GAGTC	2 cut(s)	63, 383
MroNI	GCCGGC	2 cut(s)	6, 720
MseI	TTAA	3 cut(s)	45, 485, 963
MspI	CCGG	9 cut(s)	7, 21, 237, 465, 501, 533, 572, 721, 888
MspR9I	CCNGG	6 cut(s)	21, 501, 572, 726, 777, 952
MunI	CAATTG	2 cut(s)	741, 943
MvaI	CCWGG	3 cut(s)	726, 777, 952
MvnI	CGCG	3 cut(s)	125, 138, 140
MwoI	GCNNNNNNNGC	5 cut(s)	120, 225, 254, 726, 735
NaeI	GCCGGC	2 cut(s)	8, 722
NciI	CCSGG	3 cut(s)	21, 501, 572
NdeII	GATC	8 cut(s)	186, 546, 630, 799, 803, 841, 922, 926
NgoMIV	GCCGGC	2 cut(s)	6, 720
NlaIII	CATG	3 cut(s)	166, 685, 974
NlaIV	GGNNCC	3 cut(s)	531, 714, 819
NmuCI	GTSAC	1 cut(s)	55
NspI	RCATGY	1 cut(s)	166
PauI	GCGCGC	1 cut(s)	123
PceI	AGGCCT	1 cut(s)	729
PciI	ACATGT	1 cut(s)	162
PcsI	WCGNNNNNNNCGW	5 cut(s)	375, 384, 393, 405, 931
PdiI	GCCGGC	2 cut(s)	8, 722
PfeI	GAWTC	1 cut(s)	868
PflFI	GACNNNGTC	1 cut(s)	407
PflMI	CCANNNNNTGG	1 cut(s)	731
PkrI	GCNGC	6 cut(s)	221, 250, 253, 256, 463, 643
Ple19I	CGATCG	1 cut(s)	806
PleI	GAGTC	2 cut(s)	62, 383
PmaCI	CACGTG	1 cut(s)	60
PmlI	CACGTG	1 cut(s)	60
PpsI	GAGTC	2 cut(s)	62, 383
Ppu21I	YACGTR	1 cut(s)	60
PscI	ACATGT	1 cut(s)	162
Psp6I	CCWGG	3 cut(s)	724, 775, 950
PspCI	CACGTG	1 cut(s)	60
PspGI	CCWGG	3 cut(s)	724, 775, 950
PspN4I	GGNNCC	3 cut(s)	531, 714, 819
PspPI	GGNCC	2 cut(s)	4, 23
PsuI	RGATCY	1 cut(s)	546
PsyI	GACNNNGTC	1 cut(s)	407
PteI	GCGCGC	1 cut(s)	123
PvuI	CGATCG	1 cut(s)	806
RsaI	GTAC	4 cut(s)	332, 362, 714, 882
RsaNI	GTAC	4 cut(s)	331, 361, 713, 881
SalI	GTCGAC	1 cut(s)	371
SaqAI	TTAA	3 cut(s)	45, 485, 963
SatI	GCNGC	6 cut(s)	220, 249, 252, 255, 462, 642
Sau3AI	GATC	8 cut(s)	186, 546, 630, 799, 803, 841, 922, 926
Sau96I	GGNCC	2 cut(s)	4, 23
SchI	GAGTC	2 cut(s)	63, 383
ScrFI	CCNGG	6 cut(s)	21, 501, 572, 726, 777, 952
SduI	GDGCHC	2 cut(s)	501, 598
SfcI	CTRYAG	2 cut(s)	193, 636
Sse9I	AATT	8 cut(s)	27, 281, 290, 481, 515, 741, 857, 943
SseBI	AGGCCT	1 cut(s)	729
StuI	AGGCCT	1 cut(s)	729
StyD4I	CCNGG	6 cut(s)	19, 499, 570, 724, 775, 950
StyI	CCWWGG	2 cut(s)	49, 730
TaaI	ACNGT	1 cut(s)	695
TaiI	ACGT	2 cut(s)	62, 560
TaqI	TCGA	9 cut(s)	306, 372, 378, 387, 396, 608, 647, 802, 925
TasI	AATT	8 cut(s)	27, 281, 290, 481, 515, 741, 857, 943
TauI	GCSGC	4 cut(s)	222, 251, 257, 464
TfiI	GAWTC	1 cut(s)	868
Tru1I	TTAA	3 cut(s)	45, 485, 963
Tru9I	TTAA	3 cut(s)	45, 485, 963
TseFI	GTSAC	1 cut(s)	55
TseI	GCWGC	2 cut(s)	251, 641
Tsp45I	GTSAC	1 cut(s)	55
TspDTI	ATGAA	2 cut(s)	283, 522
TspGWI	ACGGA	3 cut(s)	259, 287, 907
Tth111I	GACNNNGTC	1 cut(s)	407
Van91I	CCANNNNNTGG	1 cut(s)	731
XapI	RAATTY	3 cut(s)	281, 290, 481
XceI	RCATGY	1 cut(s)	166
XmiI	GTMKAC	1 cut(s)	372
ZraI	GACGTC	1 cut(s)	558

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.