pycom02g01770

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
N/A
Physical Location & Seq
Reverse (-)
1059304 .. 1062194
2891 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1227 bp
ATGTCGTCGATGACCAAGATCACTCATCCCCGCCAACAGATCAACGGCCAGATTTTGCCCCATCCCTGTCCCCACCTAATCGAGTTCCTCTCCAACAACGGCTCCAAGCCCTTCCGCGCCTTACAGGACTGTCTCCGGGTCAAACCGCCCGGTGGCTGCGCCTCCATCCGCCGCGATCCCAAGGAAGTCCCGCGCTGTGGCGCGTGCGGGGAGTCCGCGCGTCCCAGACTCTATGCGTGCATCACGTGCGCTGCCGTCCACTGTCACGTGCCATCCGGACCATCCCACGCGGCGGCTCACGCCCTCTGGATGCGTCCCGGTCACGAGATCGCCGTCGACGTGGACCGAGCCGAGCTCTTCTGCTGCGCGTGTAGGGACCAAGTCTACGATCGCGACTTCGACGCTGCAGTGGTCTTGGCTCAGACCGCCGCTTCGACGCTATCATCCTCCGCCGCGTCAGCTGTCCAGAAGTACGCGCCGGAGAATCTTCGGAAGCGACGCCGTATCGATTACAAGCCGTGGATGCCTGATCTGAGAGAGCAATACTTGGTGGGAAGGAATTCCAGTCCTCTAAACGGCGACGTTTCAGCTTTGCCTTGGGGATTGCGCGGGCTCAACAATCTGGGAAACACGTGCTTTATGAACTCGGTTCTGCAGGCATTGCTCCACACGCCACCGCTGCGGAACTACTTCCTCAGCGATCGGCACAACCGCTACTTCTGCCAGAAGAAGAGCAATGCCGAGAACGCGGGGAAGAAAGCCACCGCCAATAATATTGAAAGTGGCGGGAACAAAGGTGCTGCGCGCGTGTGCTTGGCCTGCGACATGGACGCCACGTTTTCGGCGGTTTTTTCAGGGGATCGATCACGCCTTATAGCCCCGCAAAGTTCTTGTACAGTTGGTGGCAGTACGCTGCGAATTTGGCGAGTTATGAGCAGCAGGACGCGCATGAGTTCTTCATTTCTATGCTTGATGGGATTCATGAGAAGGTGGATAAGGATCAGCGGAAACCCGAGAGCCAAGGCAATGGAGATTGTTGTGTTGCTCATAGAGTTTTTTCTGGTATCTTGCGATCTGATGTCATGTGTATGGCCTGTGGTTTTACGTCTACAACATATGACCCATGTGTGGACATCTCACTGGATTTGGAACCTAACCAAGGAGGTTCTGCAAAGACTGGGTCCACTAAGTCAAGTCATTCTTGCAATGGCGAGGCAGATTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UCH zf-UBP

Protein Analysis

409

Amino Acids

45.39

Weight (kDa)

9.58

Isoelectric Point (pI)

63.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBP PF02148 66 - 132 7e-10 Zn-finger in ubiquitin-hydrolases and other protein
UCH PF00443 203 - 296 3.9e-14 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 336, 384, 1108
AccIII TCCGGA 1 cut(s) 275
AclWI GGATC 3 cut(s) 170, 867, 1007
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 2 cut(s) 559, 918
AcvI CACGTG 3 cut(s) 246, 268, 633
AcyI GRCGYC 2 cut(s) 499, 831
AfaI GTAC 3 cut(s) 473, 895, 910
AfiI CCNNNNNNNGG 6 cut(s) 152, 197, 292, 575, 1128, 1159
AflIII ACRYGT 1 cut(s) 630
AgsI TTSAA 1 cut(s) 779
AjiI CACGTC 1 cut(s) 340
AluBI AGCT 3 cut(s) 355, 461, 590
AluI AGCT 3 cut(s) 355, 461, 590
Alw21I GWGCWC 1 cut(s) 357
Alw26I GTCTC 1 cut(s) 137
AlwI GGATC 3 cut(s) 170, 867, 1007
Ama87I CYCGRG 1 cut(s) 1012
Aor13HI TCCGGA 1 cut(s) 275
AoxI GGCC 3 cut(s) 46, 816, 1091
ApeKI GCWGC 8 cut(s) 156, 251, 363, 404, 679, 800, 913, 936
ApoI RAATTY 2 cut(s) 559, 918
ArsI GACNNNNNNTTYG 4 cut(s) 415, 447, 1165, 1197
Asp700I GAANNNNTTC 2 cut(s) 559, 689
AspS9I GGNCC 4 cut(s) 278, 343, 376, 1181
AsuC2I CCSGG 3 cut(s) 137, 150, 318
AvaI CYCGRG 1 cut(s) 1012
AvaII GGWCC 4 cut(s) 278, 343, 376, 1181
BanII GRGCYC 2 cut(s) 357, 615
BauI CACGAG 1 cut(s) 323
BbrPI CACGTG 3 cut(s) 246, 268, 633
Bbv12I GWGCWC 1 cut(s) 357
BbvCI CCTCAGC 1 cut(s) 695
BbvI GCAGC 8 cut(s) 143, 238, 350, 391, 666, 787, 900, 948
BccI CCATC 5 cut(s) 69, 173, 280, 289, 967
BceAI ACGGC 7 cut(s) 61, 115, 239, 317, 486, 502, 592
BcnI CCSGG 3 cut(s) 137, 150, 318
BcoDI GTCTC 1 cut(s) 137
BfmI CTRYAG 2 cut(s) 405, 653
Bme1390I CCNGG 3 cut(s) 137, 150, 318
Bme18I GGWCC 4 cut(s) 278, 343, 376, 1181
BmeT110I CYCGRG 1 cut(s) 1012
BmgBI CACGTC 1 cut(s) 340
BmgT120I GGNCC 4 cut(s) 278, 343, 376, 1181
BmiI GGNNCC 4 cut(s) 103, 377, 1151, 1182
BmrFI CCNGG 3 cut(s) 137, 150, 318
BmrI ACTGGG 1 cut(s) 1187
BmsI GCATC 3 cut(s) 249, 300, 513
BmuI ACTGGG 1 cut(s) 1187
BplI GAGNNNNNCTC 4 cut(s) 74, 106, 339, 371
Bpu10I CCTNAGC 1 cut(s) 695
BpuMI CCSGG 3 cut(s) 137, 150, 318
Bsa29I ATCGAT 2 cut(s) 507, 862
BsaAI YACGTR 3 cut(s) 246, 268, 633
BsaBI GATNNNNATC 1 cut(s) 998
BsaHI GRCGYC 2 cut(s) 499, 831
BsaJI CCNNGG 5 cut(s) 180, 518, 596, 1020, 1158
BsaWI WCCGGW 1 cut(s) 275
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 6 cut(s) 152, 197, 292, 575, 1128, 1159
Bse1I ACTGG 3 cut(s) 564, 1145, 1182
Bse3DI GCAATG 4 cut(s) 659, 742, 1032, 1212
Bse8I GATNNNNATC 1 cut(s) 998
BseAI TCCGGA 1 cut(s) 275
BseCI ATCGAT 2 cut(s) 507, 862
BseDI CCNNGG 5 cut(s) 180, 518, 596, 1020, 1158
BseGI GGATG 8 cut(s) 25, 61, 165, 272, 281, 315, 443, 528
BseJI GATNNNNATC 1 cut(s) 998
BseLI CCNNNNNNNGG 6 cut(s) 152, 197, 292, 575, 1128, 1159
BseMI GCAATG 4 cut(s) 659, 742, 1032, 1212
BseMII CTCAG 3 cut(s) 434, 524, 709
BseNI ACTGG 3 cut(s) 564, 1145, 1182
BsePI GCGCGC 1 cut(s) 803
BseXI GCAGC 8 cut(s) 143, 238, 350, 391, 666, 787, 900, 948
Bsh1285I CGRYCG 2 cut(s) 391, 703
BshFI GGCC 3 cut(s) 48, 818, 1093
BshVI ATCGAT 2 cut(s) 507, 862
BsiEI CGRYCG 2 cut(s) 391, 703
BsiHKAI GWGCWC 1 cut(s) 357
BsiHKCI CYCGRG 1 cut(s) 1012
BsiSI CCGG 5 cut(s) 136, 150, 276, 318, 479
BslFI GGGAC 5 cut(s) 54, 173, 207, 300, 389
BslI CCNNNNNNNGG 6 cut(s) 152, 197, 292, 575, 1128, 1159
BsmAI GTCTC 1 cut(s) 137
BsmFI GGGAC 5 cut(s) 54, 173, 207, 300, 389
BsnI GGCC 3 cut(s) 48, 818, 1093
BsoBI CYCGRG 1 cut(s) 1012
Bsp1286I GDGCHC 2 cut(s) 357, 615
Bsp13I TCCGGA 1 cut(s) 275
Bsp1407I TGTACA 1 cut(s) 893
Bsp68I TCGCGA 1 cut(s) 393
BspANI GGCC 3 cut(s) 48, 818, 1093
BspCNI CTCAG 3 cut(s) 433, 525, 708
BspDI ATCGAT 2 cut(s) 507, 862
BspEI TCCGGA 1 cut(s) 275
BspHI TCATGA 1 cut(s) 981
BspLI GGNNCC 4 cut(s) 103, 377, 1151, 1182
BspMAI CTGCAG 2 cut(s) 409, 657
BspPI GGATC 3 cut(s) 170, 867, 1007
BspQI GCTCTTC 2 cut(s) 362, 725
BsrDI GCAATG 4 cut(s) 659, 742, 1032, 1212
BsrGI TGTACA 1 cut(s) 893
BsrI ACTGG 3 cut(s) 564, 1145, 1182
BssECI CCNNGG 5 cut(s) 180, 518, 596, 1020, 1158
BssHII GCGCGC 1 cut(s) 803
BssNI GRCGYC 2 cut(s) 499, 831
BssSI CACGAG 1 cut(s) 323
BssT1I CCWWGG 4 cut(s) 180, 596, 1020, 1158
Bst2BI CACGAG 1 cut(s) 323
Bst4CI ACNGT 3 cut(s) 131, 263, 898
Bst6I CTCTTC 2 cut(s) 362, 725
BstACI GRCGYC 2 cut(s) 499, 831
BstAPI GCANNNNNTGC 2 cut(s) 246, 661
BstAUI TGTACA 1 cut(s) 893
BstBAI YACGTR 3 cut(s) 246, 268, 633
BstC8I GCNNGC 6 cut(s) 205, 238, 611, 657, 805, 820
BstDEI CTNAG 4 cut(s) 420, 533, 695, 1187
BstDSI CCRYGG 1 cut(s) 518
BstENI CCTNNNNNAGG 1 cut(s) 1157
BstF5I GGATG 8 cut(s) 25, 61, 165, 272, 281, 315, 443, 528
BstMAI GTCTC 1 cut(s) 137
BstMCI CGRYCG 2 cut(s) 391, 703
BstSCI CCNGG 3 cut(s) 135, 148, 316
BstSFI CTRYAG 2 cut(s) 405, 653
BstV1I GCAGC 8 cut(s) 143, 238, 350, 391, 666, 787, 900, 948
BstXI CCANNNNNNTGG 1 cut(s) 1027
Bsu15I ATCGAT 2 cut(s) 507, 862
BsuRI GGCC 3 cut(s) 48, 818, 1093
BsuTUI ATCGAT 2 cut(s) 507, 862
BtgI CCRYGG 1 cut(s) 518
BtrI CACGTC 1 cut(s) 340
BtsCI GGATG 8 cut(s) 25, 61, 165, 272, 281, 315, 443, 528
BtsI GCAGTG 1 cut(s) 414
BtsIMutI CAGTG 3 cut(s) 259, 414, 1138
BtuMI TCGCGA 1 cut(s) 393
Cac8I GCNNGC 6 cut(s) 205, 238, 611, 657, 805, 820
CciI TCATGA 1 cut(s) 981
Cfr13I GGNCC 4 cut(s) 278, 343, 376, 1181
ClaI ATCGAT 2 cut(s) 507, 862
CseI GACGC 8 cut(s) 209, 302, 410, 444, 445, 507, 839, 952
Csp6I GTAC 3 cut(s) 472, 894, 909
CviAII CATG 6 cut(s) 826, 949, 982, 1083, 1124, 1224
CviQI GTAC 3 cut(s) 472, 894, 909
DdeI CTNAG 4 cut(s) 420, 533, 695, 1187
EaeI YGGCCR 1 cut(s) 46
Eam1104I CTCTTC 2 cut(s) 362, 725
EarI CTCTTC 2 cut(s) 362, 725
EciI GGCGGA 2 cut(s) 158, 439
Ecl136II GAGCTC 1 cut(s) 355
Eco130I CCWWGG 4 cut(s) 180, 596, 1020, 1158
Eco24I GRGCYC 2 cut(s) 357, 615
Eco47I GGWCC 4 cut(s) 278, 343, 376, 1181
Eco53kI GAGCTC 1 cut(s) 355
Eco72I CACGTG 3 cut(s) 246, 268, 633
Eco88I CYCGRG 1 cut(s) 1012
EcoICRI GAGCTC 1 cut(s) 355
EcoNI CCTNNNNNAGG 1 cut(s) 1157
EcoRI GAATTC 1 cut(s) 559
EcoT14I CCWWGG 4 cut(s) 180, 596, 1020, 1158
EcoT38I GRGCYC 2 cut(s) 357, 615
ErhI CCWWGG 4 cut(s) 180, 596, 1020, 1158
FaeI CATG 6 cut(s) 829, 952, 985, 1086, 1127, 1227
FalI AAGNNNNNCTT 2 cut(s) 1185, 1217
FaqI GGGAC 5 cut(s) 54, 173, 207, 300, 389
FatI CATG 6 cut(s) 825, 948, 981, 1082, 1123, 1223
FauI CCCGC 7 cut(s) 38, 198, 200, 602, 742, 779, 888
FauNDI CATATG 1 cut(s) 1116
FblI GTMKAC 3 cut(s) 336, 384, 1108
FokI GGATG 8 cut(s) 12, 48, 152, 259, 268, 322, 430, 535
FriOI GRGCYC 2 cut(s) 357, 615
HaeIII GGCC 3 cut(s) 48, 818, 1093
HapII CCGG 5 cut(s) 136, 150, 276, 318, 479
HgaI GACGC 8 cut(s) 209, 302, 410, 444, 445, 507, 839, 952
Hin1I GRCGYC 2 cut(s) 499, 831
Hin1II CATG 6 cut(s) 829, 952, 985, 1086, 1127, 1227
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HinfI GANTC 4 cut(s) 212, 228, 484, 978
HpaII CCGG 5 cut(s) 136, 150, 276, 318, 479
Hpy166II GTNNAC 7 cut(s) 259, 337, 343, 385, 1109, 1131, 1184
Hpy188I TCNGA 4 cut(s) 423, 492, 534, 1077
Hpy188III TCNNGA 6 cut(s) 276, 307, 323, 392, 466, 982
Hpy8I GTNNAC 7 cut(s) 259, 337, 343, 385, 1109, 1131, 1184
Hpy99I CGWCG 7 cut(s) 10, 338, 341, 404, 439, 501, 584
HpyAV CCTTC 3 cut(s) 121, 549, 981
HpyCH4III ACNGT 3 cut(s) 131, 263, 898
HpyCH4IV ACGT 7 cut(s) 245, 267, 339, 582, 632, 836, 1105
HpyCH4V TGCA 6 cut(s) 240, 407, 655, 1171, 1205, 1223
HpyF3I CTNAG 4 cut(s) 420, 533, 695, 1187
HpySE526I ACGT 7 cut(s) 245, 267, 339, 582, 632, 836, 1105
Hsp92I GRCGYC 2 cut(s) 499, 831
Hsp92II CATG 6 cut(s) 829, 952, 985, 1086, 1127, 1227
Kpn2I TCCGGA 1 cut(s) 275
LguI GCTCTTC 2 cut(s) 362, 725
LmnI GCTCC 2 cut(s) 107, 669
Lsp1109I GCAGC 8 cut(s) 143, 238, 350, 391, 666, 787, 900, 948
LweI GCATC 3 cut(s) 249, 300, 513
MaeII ACGT 7 cut(s) 245, 267, 339, 582, 632, 836, 1105
MaeIII GTNAC 2 cut(s) 263, 320
MboII GAAGA 6 cut(s) 349, 479, 739, 742, 766, 948
MhlI GDGCHC 2 cut(s) 357, 615
MluCI AATT 2 cut(s) 559, 918
MlyI GAGTC 2 cut(s) 221, 222
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 8 cut(s) 98, 172, 314, 457, 579, 704, 1156, 1206
MroI TCCGGA 1 cut(s) 275
MroXI GAANNNNTTC 2 cut(s) 559, 689
MslI CAYNNNNRTG 2 cut(s) 964, 1087
MspA1I CMGCKG 3 cut(s) 461, 679, 1005
MspI CCGG 5 cut(s) 136, 150, 276, 318, 479
MspR9I CCNGG 3 cut(s) 137, 150, 318
NciI CCSGG 3 cut(s) 137, 150, 318
NdeI CATATG 1 cut(s) 1116
NlaIII CATG 6 cut(s) 829, 952, 985, 1086, 1127, 1227
NlaIV GGNNCC 4 cut(s) 103, 377, 1151, 1182
NmeAIII GCCGAG 2 cut(s) 376, 766
NmuCI GTSAC 2 cut(s) 263, 320
NruI TCGCGA 1 cut(s) 393
PagI TCATGA 1 cut(s) 981
PauI GCGCGC 1 cut(s) 803
PciSI GCTCTTC 2 cut(s) 362, 725
PcsI WCGNNNNNNNCGW 2 cut(s) 330, 336
PdmI GAANNNNTTC 2 cut(s) 559, 689
PfeI GAWTC 2 cut(s) 484, 978
PflFI GACNNNGTC 2 cut(s) 380, 1179
Ple19I CGATCG 2 cut(s) 391, 703
PleI GAGTC 2 cut(s) 220, 222
PmaCI CACGTG 3 cut(s) 246, 268, 633
PmlI CACGTG 3 cut(s) 246, 268, 633
PpsI GAGTC 2 cut(s) 220, 222
Ppu21I YACGTR 3 cut(s) 246, 268, 633
Psp124BI GAGCTC 1 cut(s) 357
PspCI CACGTG 3 cut(s) 246, 268, 633
PspN4I GGNNCC 4 cut(s) 103, 377, 1151, 1182
PspPI GGNCC 4 cut(s) 278, 343, 376, 1181
PstI CTGCAG 2 cut(s) 409, 657
PsyI GACNNNGTC 2 cut(s) 380, 1179
PteI GCGCGC 1 cut(s) 803
PvuI CGATCG 2 cut(s) 391, 703
PvuII CAGCTG 1 cut(s) 461
RruI TCGCGA 1 cut(s) 393
RsaI GTAC 3 cut(s) 473, 895, 910
RsaNI GTAC 3 cut(s) 472, 894, 909
RseI CAYNNNNRTG 2 cut(s) 964, 1087
SacI GAGCTC 1 cut(s) 357
SalI GTCGAC 1 cut(s) 335
SapI GCTCTTC 2 cut(s) 362, 725
Sau96I GGNCC 4 cut(s) 278, 343, 376, 1181
SchI GAGTC 2 cut(s) 221, 222
ScrFI CCNGG 3 cut(s) 137, 150, 318
SduI GDGCHC 2 cut(s) 357, 615
SfaNI GCATC 3 cut(s) 249, 300, 513
SfcI CTRYAG 2 cut(s) 405, 653
SgrDI CGTCGACG 1 cut(s) 335
SinI GGWCC 4 cut(s) 278, 343, 376, 1181
SmiMI CAYNNNNRTG 2 cut(s) 964, 1087
Sse9I AATT 2 cut(s) 559, 918
SspI AATATT 1 cut(s) 775
SstI GAGCTC 1 cut(s) 357
StyD4I CCNGG 3 cut(s) 135, 148, 316
StyI CCWWGG 4 cut(s) 180, 596, 1020, 1158
TaaI ACNGT 3 cut(s) 131, 263, 898
TaiI ACGT 7 cut(s) 248, 270, 342, 585, 635, 839, 1108
TaqI TCGA 7 cut(s) 8, 81, 336, 399, 434, 507, 862
TaqII GACCGA 1 cut(s) 360
TasI AATT 2 cut(s) 559, 918
TatI WGTACW 1 cut(s) 893
TauI GCSGC 5 cut(s) 174, 293, 296, 431, 455
TfiI GAWTC 2 cut(s) 484, 978
TscAI CASTG 3 cut(s) 266, 414, 1145
TseFI GTSAC 2 cut(s) 263, 320
TseI GCWGC 8 cut(s) 156, 251, 363, 404, 679, 800, 913, 936
Tsp45I GTSAC 2 cut(s) 263, 320
TspDTI ATGAA 3 cut(s) 656, 948, 970
TspRI CASTG 3 cut(s) 266, 414, 1145
Tth111I GACNNNGTC 2 cut(s) 380, 1179
VpaK11BI GGWCC 4 cut(s) 278, 343, 376, 1181
XagI CCTNNNNNAGG 1 cut(s) 1157
XapI RAATTY 2 cut(s) 559, 918
XmiI GTMKAC 3 cut(s) 336, 384, 1108
XmnI GAANNNNTTC 2 cut(s) 559, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.