pycom02g02650

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
N/A
Physical Location & Seq
Reverse (-)
1660548 .. 1660847
300 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 300 bp
ATGCATCGATGCATGCATGAAAACAAGGTGCTTTACAAGTACATACACTCCCGCCACCATAGGTTGGTGGCGCCGTACGCATATGAAGCAATGTACAACCACCCCATCGAGGGGCTGCTGGCGGACAACATGAGTGGTATTGTCAGCTTTCAGGCGTTCGGCGTGTCTCCGAGGATGTTAATTTTCTTCTCGTTCGCCACCGTCAAGGTAGTGGACGACGTGGAATGTGGATTCCGTGAAATCTATTCAAGGGTAACCATTTGTGATCCCTTTTACCTTATAACTTTCGTCGTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 map00600 map01100
Pfam Domains
Protein Families
FA_hydroxylase

Protein Analysis

100

Amino Acids

11.54

Weight (kDa)

7.0

Isoelectric Point (pI)

60.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 1 - 67 3.4e-11 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 281
AccB1I GGYRCC 1 cut(s) 70
AccB7I CCANNNNNTGG 1 cut(s) 64
AciI CCGC 2 cut(s) 52, 122
AclWI GGATC 1 cut(s) 260
AcyI GRCGYC 1 cut(s) 71
AfaI GTAC 3 cut(s) 41, 77, 95
AfiI CCNNNNNNNGG 4 cut(s) 64, 109, 110, 111
AgsI TTSAA 1 cut(s) 249
AjiI CACGTC 1 cut(s) 220
AluBI AGCT 1 cut(s) 147
AluI AGCT 1 cut(s) 147
Alw26I GTCTC 1 cut(s) 171
AlwI GGATC 1 cut(s) 260
ApeKI GCWGC 1 cut(s) 115
AspLEI GCGC 1 cut(s) 73
BanI GGYRCC 1 cut(s) 70
BbvI GCAGC 1 cut(s) 102
BccI CCATC 1 cut(s) 113
BceAI ACGGC 1 cut(s) 58
BcoDI GTCTC 1 cut(s) 171
BfoI RGCGCY 1 cut(s) 74
BisI GCNGC 1 cut(s) 116
BlsI GCNGC 1 cut(s) 117
BmgBI CACGTC 1 cut(s) 220
BmiI GGNNCC 1 cut(s) 72
BmsI GCATC 1 cut(s) 13
Bsa29I ATCGAT 1 cut(s) 7
BsaHI GRCGYC 1 cut(s) 71
BsaJI CCNNGG 1 cut(s) 170
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 109, 110, 111
Bse3DI GCAATG 1 cut(s) 96
BseCI ATCGAT 1 cut(s) 7
BseDI CCNNGG 1 cut(s) 170
BseGI GGATG 1 cut(s) 180
BseLI CCNNNNNNNGG 4 cut(s) 64, 109, 110, 111
BseMI GCAATG 1 cut(s) 96
BseXI GCAGC 1 cut(s) 102
BshNI GGYRCC 1 cut(s) 70
BshVI ATCGAT 1 cut(s) 7
BsiWI CGTACG 1 cut(s) 75
BslI CCNNNNNNNGG 4 cut(s) 64, 109, 110, 111
BsmAI GTCTC 1 cut(s) 171
Bsp1407I TGTACA 1 cut(s) 93
Bsp143I GATC 1 cut(s) 265
BspACI CCGC 2 cut(s) 52, 122
BspDI ATCGAT 1 cut(s) 7
BspLI GGNNCC 1 cut(s) 72
BspPI GGATC 1 cut(s) 260
BspT107I GGYRCC 1 cut(s) 70
BsrDI GCAATG 1 cut(s) 96
BsrGI TGTACA 1 cut(s) 93
BssECI CCNNGG 1 cut(s) 170
BssMI GATC 1 cut(s) 265
BssNI GRCGYC 1 cut(s) 71
Bst4CI ACNGT 1 cut(s) 202
BstACI GRCGYC 1 cut(s) 71
BstAUI TGTACA 1 cut(s) 93
BstC8I GCNNGC 2 cut(s) 14, 120
BstEII GGTNACC 1 cut(s) 253
BstF5I GGATG 1 cut(s) 180
BstH2I RGCGCY 1 cut(s) 74
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 1 cut(s) 268
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 1 cut(s) 265
BstMWI GCNNNNNNNGC 2 cut(s) 77, 86
BstNSI RCATGY 1 cut(s) 16
BstPI GGTNACC 1 cut(s) 253
BstV1I GCAGC 1 cut(s) 102
Bsu15I ATCGAT 1 cut(s) 7
BsuTUI ATCGAT 1 cut(s) 7
BtrI CACGTC 1 cut(s) 220
BtsCI GGATG 1 cut(s) 180
Cac8I GCNNGC 2 cut(s) 14, 120
CfoI GCGC 1 cut(s) 73
ClaI ATCGAT 1 cut(s) 7
Csp6I GTAC 3 cut(s) 40, 76, 94
CspCI CAANNNNNGTGG 2 cut(s) 115, 150
CviAII CATG 3 cut(s) 13, 17, 130
CviJI RGCY 2 cut(s) 115, 147
CviKI_1 RGCY 2 cut(s) 115, 147
CviQI GTAC 3 cut(s) 40, 76, 94
DinI GGCGCC 1 cut(s) 72
DpnI GATC 1 cut(s) 267
DpnII GATC 1 cut(s) 265
EciI GGCGGA 1 cut(s) 137
Eco91I GGTNACC 1 cut(s) 253
EcoO65I GGTNACC 1 cut(s) 253
EcoT22I ATGCAT 3 cut(s) 6, 14, 18
EgeI GGCGCC 1 cut(s) 72
EheI GGCGCC 1 cut(s) 72
FaeI CATG 3 cut(s) 16, 20, 133
FaiI YATR 9 cut(s) 14, 18, 44, 60, 82, 84, 131, 281, 298
FatI CATG 3 cut(s) 12, 16, 129
FauI CCCGC 1 cut(s) 59
FauNDI CATATG 1 cut(s) 82
Fnu4HI GCNGC 1 cut(s) 116
FokI GGATG 1 cut(s) 187
Fsp4HI GCNGC 1 cut(s) 116
GlaI GCGC 1 cut(s) 72
GluI GCNGC 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 74
HhaI GCGC 1 cut(s) 73
Hin1I GRCGYC 1 cut(s) 71
Hin1II CATG 3 cut(s) 16, 20, 133
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HinfI GANTC 1 cut(s) 231
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 1 cut(s) 171
Hpy8I GTNNAC 1 cut(s) 214
Hpy99I CGWCG 2 cut(s) 221, 293
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 3 cut(s) 4, 12, 16
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 86
HpySE526I ACGT 1 cut(s) 219
Hsp92I GRCGYC 1 cut(s) 71
Hsp92II CATG 3 cut(s) 16, 20, 133
HspAI GCGC 1 cut(s) 71
KasI GGCGCC 1 cut(s) 70
Kzo9I GATC 1 cut(s) 265
LpnPI CCDG 2 cut(s) 104, 137
Lsp1109I GCAGC 1 cut(s) 102
LweI GCATC 1 cut(s) 13
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 1 cut(s) 253
MalI GATC 1 cut(s) 267
MboI GATC 1 cut(s) 265
MboII GAAGA 1 cut(s) 178
MluCI AATT 1 cut(s) 180
Mly113I GGCGCC 1 cut(s) 71
MnlI CCTC 2 cut(s) 103, 165
Mph1103I ATGCAT 3 cut(s) 6, 14, 18
MseI TTAA 1 cut(s) 179
MwoI GCNNNNNNNGC 2 cut(s) 77, 86
NarI GGCGCC 1 cut(s) 71
NdeI CATATG 1 cut(s) 82
NdeII GATC 1 cut(s) 265
NlaIII CATG 3 cut(s) 16, 20, 133
NlaIV GGNNCC 1 cut(s) 72
NsiI ATGCAT 3 cut(s) 6, 14, 18
NspI RCATGY 1 cut(s) 16
PaeI GCATGC 1 cut(s) 16
PfeI GAWTC 1 cut(s) 231
Pfl23II CGTACG 1 cut(s) 75
PflMI CCANNNNNTGG 1 cut(s) 64
PkrI GCNGC 1 cut(s) 117
PluTI GGCGCC 1 cut(s) 74
PsiI TTATAA 1 cut(s) 281
PspEI GGTNACC 1 cut(s) 253
PspLI CGTACG 1 cut(s) 75
PspN4I GGNNCC 1 cut(s) 72
RsaI GTAC 3 cut(s) 41, 77, 95
RsaNI GTAC 3 cut(s) 40, 76, 94
SaqAI TTAA 1 cut(s) 179
SatI GCNGC 1 cut(s) 116
Sau3AI GATC 1 cut(s) 265
SetI ASST 6 cut(s) 30, 65, 149, 210, 222, 279
SfaNI GCATC 1 cut(s) 13
SfoI GGCGCC 1 cut(s) 72
SphI GCATGC 1 cut(s) 16
Sse9I AATT 1 cut(s) 180
SsiI CCGC 2 cut(s) 52, 122
SspDI GGCGCC 1 cut(s) 70
TaaI ACNGT 1 cut(s) 202
TaiI ACGT 1 cut(s) 222
TaqI TCGA 2 cut(s) 7, 108
TasI AATT 1 cut(s) 180
TatI WGTACW 2 cut(s) 39, 93
TfiI GAWTC 1 cut(s) 231
Tru1I TTAA 1 cut(s) 179
Tru9I TTAA 1 cut(s) 179
TseI GCWGC 1 cut(s) 115
TspDTI ATGAA 2 cut(s) 33, 99
TspGWI ACGGA 1 cut(s) 224
Van91I CCANNNNNTGG 1 cut(s) 64
XceI RCATGY 1 cut(s) 16
Zsp2I ATGCAT 3 cut(s) 6, 14, 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.