pycom02g02930

Nudix hydrolase 19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
N/A
Physical Location & Seq
Reverse (-)
1874436 .. 1875031
596 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 417 bp
ATGCTTTTGGTGTGTGATCAGGCCAGGGCTTTGCTAGAATGGCATAGTATATCACAATTTTGTGGACATTGTGGAGAAAAAACAGTCCCCAAGGAAGCTGGGAGGCGGAAGCAATGTTCCAGTGGGCTGTGCAGAAAGCGGGTCTACCCTCGTGTTGATCCGGTATGTTTATGCTTTATCAGGGAAAGCTTAGAAGAAGCAGTGAGGAGAGAAGCATGGGAAGAGACTGGTATTGAAGTAGGAGAAGTTGTCTATCATAGCTCTCAGCCATGGCCTGTTCCAATTTTGGTTTTGTTTCCAGTTGGACCAAATAGCATGCCAGGTCAGTTGATGGTTGGGTTCTTTGCATACGCAAAATCACTCGAAATAAATGTCGACGGGGAAGAGTTGGACGACATTATGGTTGTCTACTTGTAA

Protein Analysis

139

Amino Acids

15.59

Weight (kDa)

5.2

Isoelectric Point (pI)

51.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_NUD PF09297 18 - 41 4e-07 NADH pyrophosphatase zinc ribbon domain
NUDIX PF00293 61 - 133 1.7e-06 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 144, 375, 408
AciI CCGC 2 cut(s) 106, 139
AclWI GGATC 1 cut(s) 152
AgsI TTSAA 1 cut(s) 236
AjnI CCWGG 2 cut(s) 23, 319
AloI GAACNNNNNNTCC 2 cut(s) 100, 132
AluBI AGCT 3 cut(s) 98, 189, 261
AluI AGCT 3 cut(s) 98, 189, 261
Alw26I GTCTC 1 cut(s) 218
AlwI GGATC 1 cut(s) 152
AoxI GGCC 2 cut(s) 21, 272
ArsI GACNNNNNNTTYG 2 cut(s) 357, 389
AspS9I GGNCC 1 cut(s) 305
AvaII GGWCC 1 cut(s) 305
BauI CACGAG 1 cut(s) 150
BccI CCATC 1 cut(s) 325
BciT130I CCWGG 2 cut(s) 25, 321
BclI TGATCA 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 218
BfaI CTAG 1 cut(s) 35
Bme1390I CCNGG 2 cut(s) 25, 321
Bme18I GGWCC 1 cut(s) 305
BmgT120I GGNCC 1 cut(s) 305
BmrFI CCNGG 2 cut(s) 25, 321
BsaJI CCNNGG 3 cut(s) 24, 90, 269
BsaWI WCCGGW 1 cut(s) 160
BsaXI ACNNNNNCTCC 2 cut(s) 234, 264
Bse1I ACTGG 3 cut(s) 120, 232, 299
Bse3DI GCAATG 1 cut(s) 119
BseBI CCWGG 2 cut(s) 25, 321
BseDI CCNNGG 3 cut(s) 24, 90, 269
BseMI GCAATG 1 cut(s) 119
BseMII CTCAG 1 cut(s) 278
BseNI ACTGG 3 cut(s) 120, 232, 299
BseRI GAGGAG 1 cut(s) 220
BseYI CCCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 151
BshFI GGCC 2 cut(s) 23, 274
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 1 cut(s) 71
BsmAI GTCTC 1 cut(s) 218
BsmFI GGGAC 1 cut(s) 71
BsnI GGCC 2 cut(s) 23, 274
Bsp143I GATC 2 cut(s) 16, 157
Bsp19I CCATGG 1 cut(s) 269
BspACI CCGC 2 cut(s) 106, 139
BspANI GGCC 2 cut(s) 23, 274
BspCNI CTCAG 1 cut(s) 277
BspPI GGATC 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 119
BsrI ACTGG 3 cut(s) 120, 232, 299
BssECI CCNNGG 3 cut(s) 24, 90, 269
BssMI GATC 2 cut(s) 16, 157
BssSI CACGAG 1 cut(s) 150
BssT1I CCWWGG 2 cut(s) 90, 269
Bst2BI CACGAG 1 cut(s) 150
Bst2UI CCWGG 2 cut(s) 25, 321
Bst4CI ACNGT 1 cut(s) 85
Bst6I CTCTTC 2 cut(s) 216, 378
BstC8I GCNNGC 1 cut(s) 317
BstDEI CTNAG 2 cut(s) 190, 264
BstDSI CCRYGG 1 cut(s) 269
BstKTI GATC 2 cut(s) 19, 160
BstMAI GTCTC 1 cut(s) 218
BstMBI GATC 2 cut(s) 16, 157
BstMWI GCNNNNNNNGC 1 cut(s) 40
BstNI CCWGG 2 cut(s) 25, 321
BstNSI RCATGY 1 cut(s) 319
BstSCI CCNGG 2 cut(s) 23, 319
BsuRI GGCC 2 cut(s) 23, 274
BtgI CCRYGG 1 cut(s) 269
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 2 cut(s) 127, 207
Cac8I GCNNGC 1 cut(s) 317
Cfr13I GGNCC 1 cut(s) 305
CviAII CATG 3 cut(s) 216, 270, 316
CviJI RGCY 8 cut(s) 23, 29, 98, 127, 189, 261, 268, 274
CviKI_1 RGCY 8 cut(s) 23, 29, 98, 127, 189, 261, 268, 274
DdeI CTNAG 2 cut(s) 190, 264
DpnI GATC 2 cut(s) 18, 159
DpnII GATC 2 cut(s) 16, 157
Eam1104I CTCTTC 2 cut(s) 216, 378
EarI CTCTTC 2 cut(s) 216, 378
EciI GGCGGA 1 cut(s) 121
Eco130I CCWWGG 2 cut(s) 90, 269
Eco47I GGWCC 1 cut(s) 305
EcoRII CCWGG 2 cut(s) 23, 319
EcoT14I CCWWGG 2 cut(s) 90, 269
ErhI CCWWGG 2 cut(s) 90, 269
FaeI CATG 3 cut(s) 219, 273, 319
FaqI GGGAC 1 cut(s) 71
FatI CATG 3 cut(s) 215, 269, 315
FauI CCCGC 1 cut(s) 132
FbaI TGATCA 1 cut(s) 16
FblI GTMKAC 3 cut(s) 144, 375, 408
FspBI CTAG 1 cut(s) 35
GsaI CCCAGC 1 cut(s) 102
HaeIII GGCC 2 cut(s) 23, 274
HapII CCGG 1 cut(s) 161
Hin1II CATG 3 cut(s) 219, 273, 319
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 1 cut(s) 187
HpaII CCGG 1 cut(s) 161
Hpy166II GTNNAC 4 cut(s) 65, 145, 376, 409
Hpy8I GTNNAC 4 cut(s) 65, 145, 376, 409
Hpy99I CGWCG 1 cut(s) 380
HpyCH4III ACNGT 1 cut(s) 85
HpyCH4V TGCA 2 cut(s) 132, 347
HpyF10VI GCNNNNNNNGC 1 cut(s) 40
HpyF3I CTNAG 2 cut(s) 190, 264
Hsp92II CATG 3 cut(s) 219, 273, 319
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 2 cut(s) 16, 157
MaeI CTAG 1 cut(s) 35
MalI GATC 2 cut(s) 18, 159
MboI GATC 2 cut(s) 16, 157
MboII GAAGA 3 cut(s) 206, 233, 395
MluCI AATT 2 cut(s) 56, 282
MmeI TCCRAC 2 cut(s) 283, 369
MnlI CCTC 3 cut(s) 96, 159, 198
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 2 cut(s) 25, 321
MvaI CCWGG 2 cut(s) 25, 321
MwoI GCNNNNNNNGC 1 cut(s) 40
NcoI CCATGG 1 cut(s) 269
NdeII GATC 2 cut(s) 16, 157
NlaIII CATG 3 cut(s) 219, 273, 319
NspI RCATGY 1 cut(s) 319
PaeI GCATGC 1 cut(s) 319
Psp6I CCWGG 2 cut(s) 23, 319
PspFI CCCAGC 1 cut(s) 98
PspGI CCWGG 2 cut(s) 23, 319
PspPI GGNCC 1 cut(s) 305
SalI GTCGAC 1 cut(s) 374
Sau3AI GATC 2 cut(s) 16, 157
Sau96I GGNCC 1 cut(s) 305
ScrFI CCNGG 2 cut(s) 25, 321
SetI ASST 4 cut(s) 100, 191, 263, 325
SinI GGWCC 1 cut(s) 305
SphI GCATGC 1 cut(s) 319
Sse9I AATT 2 cut(s) 56, 282
SsiI CCGC 2 cut(s) 106, 139
SspMI CTAG 1 cut(s) 35
StyD4I CCNGG 2 cut(s) 23, 319
StyI CCWWGG 2 cut(s) 90, 269
TaaI ACNGT 1 cut(s) 85
TaqI TCGA 2 cut(s) 363, 375
TasI AATT 2 cut(s) 56, 282
TscAI CASTG 2 cut(s) 127, 207
TspRI CASTG 2 cut(s) 127, 207
VpaK11BI GGWCC 1 cut(s) 305
XceI RCATGY 1 cut(s) 319
XmiI GTMKAC 3 cut(s) 144, 375, 408
XspI CTAG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.