pycom02g04730

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0029210
Physical Location & Seq
Reverse (-)
3067853 .. 3069010
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 435 bp
ATGCTTCGTGCTTTTGGCATGGAGCTCCTTTGCAAAAGAGATAATCCGGAATACAAGTATACTATAGCAATGATGGGTTATGGTCCCGAAGACAAAAGCGCCGTTCTGGAGTTGACATACAATTATGGGGTCACCGAATATAACAAAGGAAATGCTTATGCTCAGATAGCAATAGGCACAGATGATGTCTATAAAACTGCCGAAGCAATTAAACTCTATGGGGGCAAGATTACCCGGGAACCTGGACCATTACCTGGTCTCAACACGAAGATCACTGCTTGCTTGGATCCTGACGGATGGAAGTCGGTTTTCGTGGATAATGTTGATTTCCTCAAGGAATTGGACTCGGGCCAAGATGATTCTATGAAGCTGGTTCTCCTGAACGTACGTGTTGAATTCTGTTTTGTAGATAAAATTATAATTAGCTTTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

145

Amino Acids

16.16

Weight (kDa)

4.73

Isoelectric Point (pI)

17.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 4 - 101 5.6e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0029210)

Species Orthologous Gene IDs
pyrus_communis pycom02g04730
rosa_multiflora Rmu_co8095376.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 419
AccB7I CCANNNNNTGG 1 cut(s) 254
AccI GTMKAC 1 cut(s) 59
AccIII TCCGGA 1 cut(s) 46
AclWI GGATC 2 cut(s) 281, 294
AcsI RAATTY 1 cut(s) 395
AfaI GTAC 1 cut(s) 387
AfiI CCNNNNNNNGG 1 cut(s) 254
AflIII ACRYGT 1 cut(s) 388
AgsI TTSAA 2 cut(s) 395, 431
AjnI CCWGG 2 cut(s) 241, 253
AluBI AGCT 3 cut(s) 25, 370, 426
AluI AGCT 3 cut(s) 25, 370, 426
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 2 cut(s) 281, 294
Ama87I CYCGRG 2 cut(s) 234, 346
Aor13HI TCCGGA 1 cut(s) 46
AoxI GGCC 1 cut(s) 349
ApoI RAATTY 1 cut(s) 395
AspLEI GCGC 1 cut(s) 101
AspS9I GGNCC 3 cut(s) 83, 245, 349
AsuC2I CCSGG 2 cut(s) 235, 236
AsuHPI GGTGA 1 cut(s) 124
AvaI CYCGRG 2 cut(s) 234, 346
AvaII GGWCC 2 cut(s) 83, 245
BamHI GGATCC 1 cut(s) 286
BanII GRGCYC 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 27
BccI CCATC 2 cut(s) 67, 291
BceAI ACGGC 1 cut(s) 86
BciT130I CCWGG 2 cut(s) 243, 255
BcnI CCSGG 2 cut(s) 235, 236
BcoDI GTCTC 1 cut(s) 263
BfmI CTRYAG 1 cut(s) 63
BfoI RGCGCY 1 cut(s) 102
Bme1390I CCNGG 4 cut(s) 235, 236, 243, 255
Bme18I GGWCC 2 cut(s) 83, 245
BmeT110I CYCGRG 2 cut(s) 234, 346
BmgT120I GGNCC 3 cut(s) 83, 245, 349
BmiI GGNNCC 3 cut(s) 85, 240, 288
BmrFI CCNGG 4 cut(s) 235, 236, 243, 255
BpiI GAAGAC 1 cut(s) 96
BpmI CTGGAG 1 cut(s) 128
BpuEI CTTGAG 1 cut(s) 317
BpuMI CCSGG 2 cut(s) 235, 236
BsaAI YACGTR 1 cut(s) 389
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 1 cut(s) 234
BsaWI WCCGGW 1 cut(s) 46
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse3DI GCAATG 1 cut(s) 75
BseAI TCCGGA 1 cut(s) 46
BseBI CCWGG 2 cut(s) 243, 255
BseDI CCNNGG 1 cut(s) 234
BseGI GGATG 1 cut(s) 302
BseLI CCNNNNNNNGG 1 cut(s) 254
BseMI GCAATG 1 cut(s) 75
BseMII CTCAG 1 cut(s) 176
BshFI GGCC 1 cut(s) 351
BsiHKAI GWGCWC 1 cut(s) 27
BsiHKCI CYCGRG 2 cut(s) 234, 346
BsiSI CCGG 2 cut(s) 47, 235
BsiWI CGTACG 1 cut(s) 385
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 1 cut(s) 254
BsmAI GTCTC 1 cut(s) 263
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 1 cut(s) 351
Bso31I GGTCTC 1 cut(s) 263
BsoBI CYCGRG 2 cut(s) 234, 346
Bsp1286I GDGCHC 1 cut(s) 27
Bsp13I TCCGGA 1 cut(s) 46
Bsp143I GATC 2 cut(s) 270, 286
BspANI GGCC 1 cut(s) 351
BspCNI CTCAG 1 cut(s) 175
BspEI TCCGGA 1 cut(s) 46
BspLI GGNNCC 3 cut(s) 85, 240, 288
BspPI GGATC 2 cut(s) 281, 294
BspTNI GGTCTC 1 cut(s) 263
BsrDI GCAATG 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 234
BssMI GATC 2 cut(s) 270, 286
BssNAI GTATAC 1 cut(s) 60
Bst1107I GTATAC 1 cut(s) 60
Bst2UI CCWGG 2 cut(s) 243, 255
BstBAI YACGTR 1 cut(s) 389
BstC8I GCNNGC 1 cut(s) 280
BstDEI CTNAG 1 cut(s) 162
BstEII GGTNACC 1 cut(s) 130
BstF5I GGATG 1 cut(s) 302
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 2 cut(s) 273, 289
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 2 cut(s) 270, 286
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstNI CCWGG 2 cut(s) 243, 255
BstPI GGTNACC 1 cut(s) 130
BstSCI CCNGG 4 cut(s) 233, 234, 241, 253
BstSFI CTRYAG 1 cut(s) 63
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
BstZ17I GTATAC 1 cut(s) 60
BsuRI GGCC 1 cut(s) 351
BtsCI GGATG 1 cut(s) 302
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 273
Cac8I GCNNGC 1 cut(s) 280
CfoI GCGC 1 cut(s) 101
Cfr13I GGNCC 3 cut(s) 83, 245, 349
Cfr9I CCCGGG 1 cut(s) 234
CsiI ACCWGGT 1 cut(s) 253
Csp6I GTAC 1 cut(s) 386
CviAII CATG 1 cut(s) 19
CviJI RGCY 4 cut(s) 25, 351, 370, 426
CviKI_1 RGCY 4 cut(s) 25, 351, 370, 426
CviQI GTAC 1 cut(s) 386
DdeI CTNAG 1 cut(s) 162
DpnI GATC 2 cut(s) 272, 288
DpnII GATC 2 cut(s) 270, 286
Ecl136II GAGCTC 1 cut(s) 25
Eco24I GRGCYC 1 cut(s) 27
Eco31I GGTCTC 1 cut(s) 263
Eco47I GGWCC 2 cut(s) 83, 245
Eco53kI GAGCTC 1 cut(s) 25
Eco88I CYCGRG 2 cut(s) 234, 346
Eco91I GGTNACC 1 cut(s) 130
EcoICRI GAGCTC 1 cut(s) 25
EcoO65I GGTNACC 1 cut(s) 130
EcoRI GAATTC 1 cut(s) 395
EcoRII CCWGG 2 cut(s) 241, 253
EcoT38I GRGCYC 1 cut(s) 27
FaeI CATG 1 cut(s) 22
FaqI GGGAC 1 cut(s) 69
FatI CATG 1 cut(s) 18
FblI GTMKAC 1 cut(s) 59
FokI GGATG 1 cut(s) 309
FriOI GRGCYC 1 cut(s) 27
GlaI GCGC 1 cut(s) 100
GsuI CTGGAG 1 cut(s) 128
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 1 cut(s) 351
HapII CCGG 2 cut(s) 47, 235
HhaI GCGC 1 cut(s) 101
Hin1II CATG 1 cut(s) 22
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HinfI GANTC 2 cut(s) 344, 359
HpaII CCGG 2 cut(s) 47, 235
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 2 cut(s) 60, 114
Hpy188I TCNGA 1 cut(s) 165
Hpy188III TCNNGA 5 cut(s) 47, 86, 107, 290, 379
Hpy8I GTNNAC 2 cut(s) 60, 114
HpyCH4IV ACGT 2 cut(s) 384, 388
HpyCH4V TGCA 1 cut(s) 33
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpyF3I CTNAG 1 cut(s) 162
HpySE526I ACGT 2 cut(s) 384, 388
Hsp92II CATG 1 cut(s) 22
HspAI GCGC 1 cut(s) 99
Kpn2I TCCGGA 1 cut(s) 46
Kzo9I GATC 2 cut(s) 270, 286
LmnI GCTCC 2 cut(s) 22, 30
MabI ACCWGGT 1 cut(s) 253
MaeII ACGT 2 cut(s) 384, 388
MaeIII GTNAC 1 cut(s) 130
MalI GATC 2 cut(s) 272, 288
MboI GATC 2 cut(s) 270, 286
MboII GAAGA 2 cut(s) 101, 280
MflI RGATCY 1 cut(s) 286
MhlI GDGCHC 1 cut(s) 27
MluCI AATT 6 cut(s) 121, 207, 338, 395, 414, 420
MlyI GAGTC 1 cut(s) 338
MnlI CCTC 1 cut(s) 341
MroI TCCGGA 1 cut(s) 46
MseI TTAA 1 cut(s) 210
MspI CCGG 2 cut(s) 47, 235
MspR9I CCNGG 4 cut(s) 235, 236, 243, 255
MvaI CCWGG 2 cut(s) 243, 255
MwoI GCNNNNNNNGC 1 cut(s) 167
NciI CCSGG 2 cut(s) 235, 236
NdeII GATC 2 cut(s) 270, 286
NlaIII CATG 1 cut(s) 22
NlaIV GGNNCC 3 cut(s) 85, 240, 288
NmuCI GTSAC 1 cut(s) 130
PfeI GAWTC 1 cut(s) 359
Pfl23II CGTACG 1 cut(s) 385
PflMI CCANNNNNTGG 1 cut(s) 254
PleI GAGTC 1 cut(s) 338
PpsI GAGTC 1 cut(s) 338
Ppu21I YACGTR 1 cut(s) 389
PsiI TTATAA 1 cut(s) 419
Psp124BI GAGCTC 1 cut(s) 27
Psp6I CCWGG 2 cut(s) 241, 253
PspEI GGTNACC 1 cut(s) 130
PspGI CCWGG 2 cut(s) 241, 253
PspLI CGTACG 1 cut(s) 385
PspN4I GGNNCC 3 cut(s) 85, 240, 288
PspPI GGNCC 3 cut(s) 83, 245, 349
PsuI RGATCY 1 cut(s) 286
RsaI GTAC 1 cut(s) 387
RsaNI GTAC 1 cut(s) 386
SacI GAGCTC 1 cut(s) 27
SaqAI TTAA 1 cut(s) 210
Sau3AI GATC 2 cut(s) 270, 286
Sau96I GGNCC 3 cut(s) 83, 245, 349
SchI GAGTC 1 cut(s) 338
ScrFI CCNGG 4 cut(s) 235, 236, 243, 255
SduI GDGCHC 1 cut(s) 27
SetI ASST 7 cut(s) 27, 244, 256, 372, 387, 391, 428
SexAI ACCWGGT 1 cut(s) 253
SfcI CTRYAG 1 cut(s) 63
SinI GGWCC 2 cut(s) 83, 245
SmaI CCCGGG 1 cut(s) 236
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
Sse9I AATT 6 cut(s) 121, 207, 338, 395, 414, 420
SstI GAGCTC 1 cut(s) 27
StyD4I CCNGG 4 cut(s) 233, 234, 241, 253
TaiI ACGT 2 cut(s) 387, 391
TasI AATT 6 cut(s) 121, 207, 338, 395, 414, 420
TfiI GAWTC 1 cut(s) 359
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 1 cut(s) 280
TseFI GTSAC 1 cut(s) 130
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 1 cut(s) 380
TspGWI ACGGA 1 cut(s) 309
TspMI CCCGGG 1 cut(s) 234
TspRI CASTG 1 cut(s) 280
Van91I CCANNNNNTGG 1 cut(s) 254
VpaK11BI GGWCC 2 cut(s) 83, 245
XapI RAATTY 1 cut(s) 395
XmaI CCCGGG 1 cut(s) 234
XmiI GTMKAC 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.