pycom02g06190

Glutamate-rich WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
N/A
Physical Location & Seq
Forward (+)
4233056 .. 4233927
872 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 318 bp
ATGGTCAGAAGCATCAAGAACCCTAAGAAAGCCAAAAGAAAGGCCAAGGGCTCAAAGAAAGGAGATGCGTCTTCTTCCTCTTCGGGACCGTCGATACCGGCGAAGGTATGGCAACCGGGAGTGGACAAGCTGGAGGAGGGTGAAGAGCTCCGGTGTGACCCTTCAGCTTACAATTCTCTCCATGCCTTCCATATCGGTTGGCCATGTTTAAGCTTCGATATTGTGCGAGAGACATTGGGGTTGGTTCGGACAGAGTTCCCCCATACCGTGTATTTCGTTGCAGGGACTCAGGCAGAAAAAGCTGCTTGGAATACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CAF1C_H4-bd WD40

Protein Analysis

106

Amino Acids

11.53

Weight (kDa)

9.39

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1C_H4-bd PF12265 48 - 104 1.8e-10 Histone-binding protein RBBP4 or subunit C of CAF1 complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 147
AluBI AGCT 5 cut(s) 130, 148, 167, 213, 302
AluI AGCT 5 cut(s) 130, 148, 167, 213, 302
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 1 cut(s) 224
AoxI GGCC 2 cut(s) 42, 200
ApeKI GCWGC 1 cut(s) 302
AspS9I GGNCC 1 cut(s) 86
AsuC2I CCSGG 1 cut(s) 117
AsuHPI GGTGA 1 cut(s) 152
AvaII GGWCC 1 cut(s) 86
BalI TGGCCA 1 cut(s) 202
BanII GRGCYC 2 cut(s) 53, 150
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 1 cut(s) 289
BcnI CCSGG 1 cut(s) 117
BcoDI GTCTC 1 cut(s) 224
BisI GCNGC 1 cut(s) 303
BlsI GCNGC 1 cut(s) 304
Bme1390I CCNGG 1 cut(s) 117
Bme18I GGWCC 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 1 cut(s) 117
BmsI GCATC 2 cut(s) 21, 55
BpiI GAAGAC 1 cut(s) 63
BpmI CTGGAG 1 cut(s) 152
BpuMI CCSGG 1 cut(s) 117
BsaJI CCNNGG 1 cut(s) 45
BsaWI WCCGGW 1 cut(s) 150
Bse118I RCCGGY 1 cut(s) 97
BseDI CCNNGG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 149
BseXI GCAGC 1 cut(s) 289
BshFI GGCC 2 cut(s) 44, 202
BsiHKAI GWGCWC 1 cut(s) 150
BsiSI CCGG 3 cut(s) 98, 116, 151
BslFI GGGAC 2 cut(s) 99, 298
BsmAI GTCTC 1 cut(s) 224
BsmFI GGGAC 2 cut(s) 99, 298
BsnI GGCC 2 cut(s) 44, 202
Bsp1286I GDGCHC 2 cut(s) 53, 150
BspANI GGCC 2 cut(s) 44, 202
BspCNI CTCAG 1 cut(s) 301
BspLI GGNNCC 1 cut(s) 87
BspQI GCTCTTC 1 cut(s) 138
BsrFI RCCGGY 1 cut(s) 97
BssAI RCCGGY 1 cut(s) 97
BssECI CCNNGG 1 cut(s) 45
BssT1I CCWWGG 1 cut(s) 45
Bst4CI ACNGT 2 cut(s) 90, 268
Bst6I CTCTTC 2 cut(s) 85, 138
BstDEI CTNAG 2 cut(s) 24, 288
BstMAI GTCTC 1 cut(s) 224
BstMWI GCNNNNNNNGC 1 cut(s) 299
BstSCI CCNGG 1 cut(s) 115
BstV1I GCAGC 1 cut(s) 289
BstV2I GAAGAC 1 cut(s) 63
BsuRI GGCC 2 cut(s) 44, 202
Cfr10I RCCGGY 1 cut(s) 97
Cfr13I GGNCC 1 cut(s) 86
CseI GACGC 1 cut(s) 57
CviAII CATG 2 cut(s) 182, 204
CviJI RGCY 9 cut(s) 32, 44, 51, 130, 148, 167, 202, 213, 302
CviKI_1 RGCY 9 cut(s) 32, 44, 51, 130, 148, 167, 202, 213, 302
DdeI CTNAG 2 cut(s) 24, 288
EaeI YGGCCR 1 cut(s) 200
Eam1104I CTCTTC 2 cut(s) 85, 138
EarI CTCTTC 2 cut(s) 85, 138
Ecl136II GAGCTC 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 45
Eco24I GRGCYC 2 cut(s) 53, 150
Eco47I GGWCC 1 cut(s) 86
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 147
EcoICRI GAGCTC 1 cut(s) 148
EcoT14I CCWWGG 1 cut(s) 45
EcoT38I GRGCYC 2 cut(s) 53, 150
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 2 cut(s) 185, 207
FaiI YATR 5 cut(s) 109, 183, 192, 205, 264
FaqI GGGAC 2 cut(s) 99, 298
FatI CATG 2 cut(s) 181, 203
Fnu4HI GCNGC 1 cut(s) 303
FriOI GRGCYC 2 cut(s) 53, 150
Fsp4HI GCNGC 1 cut(s) 303
GluI GCNGC 1 cut(s) 303
GsuI CTGGAG 1 cut(s) 152
HaeIII GGCC 2 cut(s) 44, 202
HapII CCGG 3 cut(s) 98, 116, 151
HgaI GACGC 1 cut(s) 57
Hin1II CATG 2 cut(s) 185, 207
HindIII AAGCTT 1 cut(s) 211
HinfI GANTC 1 cut(s) 286
HpaII CCGG 3 cut(s) 98, 116, 151
HphI GGTGA 1 cut(s) 152
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 2 cut(s) 8, 249
Hpy188III TCNNGA 2 cut(s) 16, 84
Hpy8I GTNNAC 1 cut(s) 124
Hpy99I CGWCG 1 cut(s) 94
HpyAV CCTTC 3 cut(s) 97, 171, 196
HpyCH4III ACNGT 2 cut(s) 90, 268
HpyCH4V TGCA 1 cut(s) 281
HpyF10VI GCNNNNNNNGC 1 cut(s) 299
HpyF3I CTNAG 2 cut(s) 24, 288
Hsp92II CATG 2 cut(s) 185, 207
LguI GCTCTTC 1 cut(s) 138
LmnI GCTCC 1 cut(s) 153
LpnPI CCDG 6 cut(s) 111, 116, 129, 164, 267, 275
Lsp1109I GCAGC 1 cut(s) 289
LweI GCATC 2 cut(s) 21, 55
MaeIII GTNAC 1 cut(s) 155
MboII GAAGA 4 cut(s) 63, 66, 72, 155
MhlI GDGCHC 2 cut(s) 53, 150
MlsI TGGCCA 1 cut(s) 202
MluCI AATT 1 cut(s) 172
MluNI TGGCCA 1 cut(s) 202
MlyI GAGTC 1 cut(s) 280
MnlI CCTC 3 cut(s) 88, 127, 130
Mox20I TGGCCA 1 cut(s) 202
MscI TGGCCA 1 cut(s) 202
MseI TTAA 2 cut(s) 209, 316
Msp20I TGGCCA 1 cut(s) 202
MspI CCGG 3 cut(s) 98, 116, 151
MspR9I CCNGG 1 cut(s) 117
MwoI GCNNNNNNNGC 1 cut(s) 299
NciI CCSGG 1 cut(s) 117
NlaIII CATG 2 cut(s) 185, 207
NlaIV GGNNCC 1 cut(s) 87
NmuCI GTSAC 1 cut(s) 155
PciSI GCTCTTC 1 cut(s) 138
PcsI WCGNNNNNNNCGW 2 cut(s) 89, 98
PkrI GCNGC 1 cut(s) 304
PleI GAGTC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 280
Psp124BI GAGCTC 1 cut(s) 150
PspN4I GGNNCC 1 cut(s) 87
PspPI GGNCC 1 cut(s) 86
SacI GAGCTC 1 cut(s) 150
SapI GCTCTTC 1 cut(s) 138
SaqAI TTAA 2 cut(s) 209, 316
SatI GCNGC 1 cut(s) 303
Sau96I GGNCC 1 cut(s) 86
SchI GAGTC 1 cut(s) 280
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 2 cut(s) 53, 150
SetI ASST 6 cut(s) 108, 132, 150, 169, 215, 304
SfaNI GCATC 2 cut(s) 21, 55
SinI GGWCC 1 cut(s) 86
Sse9I AATT 1 cut(s) 172
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 1 cut(s) 115
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 2 cut(s) 90, 268
TaqI TCGA 2 cut(s) 92, 216
TasI AATT 1 cut(s) 172
Tru1I TTAA 2 cut(s) 209, 316
Tru9I TTAA 2 cut(s) 209, 316
TseFI GTSAC 1 cut(s) 155
TseI GCWGC 1 cut(s) 302
Tsp45I GTSAC 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.