pycom02g06420

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
N/A
Physical Location & Seq
Reverse (-)
4471158 .. 4479134
7977 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1629 bp
ATGCGAGCGTACATAGAAGTGGCTGTTTGCTTAATGTCTATTGCACTAGTTTCAGTAGTTCCGCAACAGAAAATCGTTATACCCAACAAACATGGCGAAAAGCTTGTGGGCATATTACATGAAACTGGTTCTGCGGAGATTGTAATCTTATGCCATGGTTTTCGAGCCTCCAAGGAAGAAACAACTATGGTGAACCTTGCTGTTGCATTGGAAAAGGAAGGAATTAGTTCCTTCCGTTTCGACTTTGCTGGAAATGGAGAGAGTGAAGGCACCTTTCTGTATGGCAACTATCGGAGAGAGGCCGAGGACTTGCATGCTGTGGTCCAACACTTCTCTAGGGCAAACCGGCCACCAACTGCAATTCTTGGGCACAGTAAAGGAGCTGGTGTGGTGCTGCTGTATGCTTCTAAGTATCATGACATTCGTACGGTTGTCAATCTTTCTGGACGTTATGATCTGAAGAAAGGCATTGCACAACAATTTGGGAAAGACTTCATGGAAATAATCAAGAAGGAAGGATTCATTGATGTTAAAACTAAGACAGGAAGTGTGATTTACCGTGTGACCGAGGAAGGTCTGATGGATCGCCTAAGTACTGATATGCACGAATCATGCCTTCATATTGACAAAGAATGCCGCATGACCGAGTTCATGGACTATTCTGTACAAAGGGTGTTGACAGTCCATGGTTCTGCTGATGAGGCCATCCCTGTCGAAGATGCATTCGAGTTTTCAAAGATAATACCAAACCACAAATTACATGTTATCGAAGGAGCTAACCATTGTTACACCTCACATCAAGCTGAGTTAGCGACAGTCGTTTTGGACTTCATAAAAGCAGCTCTGCAGCAGGACAAGAAAATCATCGTACCCAACAAACACGGTGAAAAGCTTGTGGGATTGTTACACGAAACCGGTTCCGCGGACCTCGTAATCTTATGTCATGGATTCCGAGACACCGAGGAAAACTATATTATGGTGAACCTTGCTGTTGCACTGGAAAATGAAGGGATCAGTTCCTTCCGTTTTGACTTTGCCGGAAATGGGGAAAGTGAAGGCACCTTTCAGTTTGGTAACTATCGGAGAGAGGTGGATGACTTGCATTCTGTGGTCGAGTACTTCTCTGGGGCAAAACGTGAACCCAGTGCAATTGTTGGACACAGTAAAGGAGGCGATGCTGTGCTCCTGTATGCTTCTACGTATCATGACATTCGTACGGTTGTCAATGTTTGTGGTCGTTATGATATGAAGAAGGGCATCGAAGAATGCTTGGGGAAAGACTTTATGGAAGTAATCAAGAAGGAAGGGTTCATTGATGTTAAGAATAAGTCAGGAGATGGTAATTATCGTGTGACCGAGGAGAGTTTGATGGATCGCCTAAGTACTGATATGCACGAATCATGCCTTCAGATTGACAAAGAATGCCGGGTGGTGACAATCCATGGAACTGCCGACGAGGTCATCCCTGTTGAAGATGCATTAGAGTTTGCCAAGATAATACCTAACCACAAATTACATCTTATAGAAGGCGCTAATCATTCGTACACCTCGCATCAAGCCGAGTTAGCATCGCTCGTCGTGGACTTCATCAAGGCAGCTCTGCAGCAGGACAAGGCTACTTCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

543

Amino Acids

60.33

Weight (kDa)

5.66

Isoelectric Point (pI)

34.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 46 - 262 2.2e-15 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 47 - 149 7.4e-08 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 48 - 271 6.4e-08 Alpha/beta hydrolase family
Hydrolase_4 PF12146 310 - 419 6.8e-13 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 269, 1058
AccII CGCG 1 cut(s) 923
AciI CCGC 5 cut(s) 62, 134, 637, 921, 923
AclWI GGATC 3 cut(s) 591, 1019, 1380
AcoI YGGCCR 1 cut(s) 347
AcuI CTGAAG 2 cut(s) 479, 1391
AfaI GTAC 9 cut(s) 11, 427, 595, 666, 870, 1118, 1216, 1384, 1544
AfiI CCNNNNNNNGG 1 cut(s) 1044
AflIII ACRYGT 1 cut(s) 760
AgeI ACCGGT 1 cut(s) 914
AgsI TTSAA 3 cut(s) 735, 1472, 1623
AhlI ACTAGT 1 cut(s) 46
AluBI AGCT 7 cut(s) 103, 383, 776, 803, 842, 892, 1598
AluI AGCT 7 cut(s) 103, 383, 776, 803, 842, 892, 1598
Alw21I GWGCWC 1 cut(s) 1185
Alw26I GTCTC 1 cut(s) 948
AlwI GGATC 3 cut(s) 591, 1019, 1380
AoxI GGCC 3 cut(s) 300, 347, 702
ApeKI GCWGC 5 cut(s) 394, 839, 847, 1595, 1603
ArsI GACNNNNNNTTYG 2 cut(s) 805, 837
AsiGI ACCGGT 1 cut(s) 914
Asp700I GAANNNNTTC 2 cut(s) 226, 491
AspLEI GCGC 1 cut(s) 1532
AspS9I GGNCC 2 cut(s) 322, 925
AsuC2I CCSGG 1 cut(s) 1427
AsuHPI GGTGA 4 cut(s) 202, 896, 991, 1444
AvaII GGWCC 2 cut(s) 322, 925
BaeGI GKGCMC 1 cut(s) 372
BanI GGYRCC 2 cut(s) 269, 1058
Bbv12I GWGCWC 1 cut(s) 1185
BbvI GCAGC 5 cut(s) 381, 851, 859, 1607, 1615
BccI CCATC 4 cut(s) 574, 713, 1331, 1363
BcnI CCSGG 1 cut(s) 1427
BcoDI GTCTC 1 cut(s) 948
BcuI ACTAGT 1 cut(s) 46
BfaI CTAG 3 cut(s) 47, 336, 1627
BfmI CTRYAG 2 cut(s) 845, 1601
BfoI RGCGCY 1 cut(s) 1533
BisI GCNGC 6 cut(s) 395, 637, 840, 848, 1596, 1604
BlsI GCNGC 6 cut(s) 396, 638, 841, 849, 1597, 1605
BmcAI AGTACT 3 cut(s) 595, 1118, 1384
Bme1390I CCNGG 1 cut(s) 1427
Bme18I GGWCC 2 cut(s) 322, 925
BmgT120I GGNCC 2 cut(s) 322, 925
BmiI GGNNCC 3 cut(s) 271, 919, 1060
BmrFI CCNGG 1 cut(s) 1427
BmrI ACTGGG 1 cut(s) 1137
BmsI GCATC 6 cut(s) 709, 1165, 1266, 1465, 1561, 1577
BmuI ACTGGG 1 cut(s) 1137
BplI GAGNNNNNCTC 2 cut(s) 1106, 1138
BpuMI CCSGG 1 cut(s) 1427
BsaAI YACGTR 1 cut(s) 1200
BsaBI GATNNNNATC 1 cut(s) 143
BsaJI CCNNGG 9 cut(s) 154, 171, 303, 567, 685, 921, 960, 1356, 1441
BsaWI WCCGGW 1 cut(s) 914
BsaXI ACNNNNNCTCC 2 cut(s) 372, 402
Bsc4I CCNNNNNNNGG 1 cut(s) 1044
Bse118I RCCGGY 2 cut(s) 345, 914
Bse1I ACTGG 3 cut(s) 130, 1002, 1143
Bse3DI GCAATG 1 cut(s) 468
Bse8I GATNNNNATC 1 cut(s) 143
BseDI CCNNGG 9 cut(s) 154, 171, 303, 567, 685, 921, 960, 1356, 1441
BseGI GGATG 3 cut(s) 705, 1099, 1461
BseJI GATNNNNATC 1 cut(s) 143
BseLI CCNNNNNNNGG 1 cut(s) 1044
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 1 cut(s) 795
BseNI ACTGG 3 cut(s) 130, 1002, 1143
BseRI GAGGAG 1 cut(s) 1373
BseSI GKGCMC 1 cut(s) 372
BseXI GCAGC 5 cut(s) 381, 851, 859, 1607, 1615
Bsh1236I CGCG 1 cut(s) 923
BshFI GGCC 3 cut(s) 302, 349, 704
BshNI GGYRCC 2 cut(s) 269, 1058
BshTI ACCGGT 1 cut(s) 914
BsiHKAI GWGCWC 1 cut(s) 1185
BsiSI CCGG 4 cut(s) 346, 915, 1038, 1426
BsiWI CGTACG 2 cut(s) 425, 1214
BslI CCNNNNNNNGG 1 cut(s) 1044
BsmAI GTCTC 1 cut(s) 948
BsmI GAATGC 5 cut(s) 638, 722, 1102, 1271, 1427
BsnI GGCC 3 cut(s) 302, 349, 704
Bsp1286I GDGCHC 2 cut(s) 372, 1185
Bsp1407I TGTACA 1 cut(s) 664
Bsp143I GATC 4 cut(s) 454, 583, 1011, 1372
Bsp19I CCATGG 3 cut(s) 154, 685, 1441
BspACI CCGC 5 cut(s) 62, 134, 637, 921, 923
BspANI GGCC 3 cut(s) 302, 349, 704
BspCNI CTCAG 1 cut(s) 796
BspFNI CGCG 1 cut(s) 923
BspHI TCATGA 2 cut(s) 415, 1204
BspLI GGNNCC 3 cut(s) 271, 919, 1060
BspMAI CTGCAG 2 cut(s) 849, 1605
BspPI GGATC 3 cut(s) 591, 1019, 1380
BspT107I GGYRCC 2 cut(s) 269, 1058
BsrDI GCAATG 1 cut(s) 468
BsrFI RCCGGY 2 cut(s) 345, 914
BsrGI TGTACA 1 cut(s) 664
BsrI ACTGG 3 cut(s) 130, 1002, 1143
BssAI RCCGGY 2 cut(s) 345, 914
BssECI CCNNGG 9 cut(s) 154, 171, 303, 567, 685, 921, 960, 1356, 1441
BssMI GATC 4 cut(s) 454, 583, 1011, 1372
BssT1I CCWWGG 4 cut(s) 154, 171, 685, 1441
Bst4CI ACNGT 8 cut(s) 374, 430, 560, 682, 817, 884, 1163, 1219
BstAUI TGTACA 1 cut(s) 664
BstBAI YACGTR 1 cut(s) 1200
BstC8I GCNNGC 2 cut(s) 6, 315
BstDEI CTNAG 5 cut(s) 408, 537, 590, 804, 1379
BstDSI CCRYGG 4 cut(s) 154, 685, 921, 1441
BstF5I GGATG 3 cut(s) 705, 1099, 1461
BstFNI CGCG 1 cut(s) 923
BstH2I RGCGCY 1 cut(s) 1533
BstHHI GCGC 1 cut(s) 1532
BstKTI GATC 4 cut(s) 457, 586, 1014, 1375
BstMAI GTCTC 1 cut(s) 948
BstMBI GATC 4 cut(s) 454, 583, 1011, 1372
BstMWI GCNNNNNNNGC 3 cut(s) 701, 809, 1565
BstNSI RCATGY 2 cut(s) 317, 764
BstSCI CCNGG 1 cut(s) 1425
BstSFI CTRYAG 2 cut(s) 845, 1601
BstSLI GKGCMC 1 cut(s) 372
BstSNI TACGTA 1 cut(s) 1200
BstUI CGCG 1 cut(s) 923
BstV1I GCAGC 5 cut(s) 381, 851, 859, 1607, 1615
BsuRI GGCC 3 cut(s) 302, 349, 704
BtgI CCRYGG 4 cut(s) 154, 685, 921, 1441
BtgZI GCGATG 2 cut(s) 1188, 1554
BtsCI GGATG 3 cut(s) 705, 1099, 1461
BtsIMutI CAGTG 2 cut(s) 995, 1150
Cac8I GCNNGC 2 cut(s) 6, 315
CciI TCATGA 2 cut(s) 415, 1204
CfoI GCGC 1 cut(s) 1532
Cfr10I RCCGGY 2 cut(s) 345, 914
Cfr13I GGNCC 2 cut(s) 322, 925
Cfr42I CCGCGG 1 cut(s) 924
Csp6I GTAC 9 cut(s) 10, 426, 594, 665, 869, 1117, 1215, 1383, 1543
CspAI ACCGGT 1 cut(s) 914
CspCI CAANNNNNGTGG 2 cut(s) 1213, 1248
CviQI GTAC 9 cut(s) 10, 426, 594, 665, 869, 1117, 1215, 1383, 1543
DdeI CTNAG 5 cut(s) 408, 537, 590, 804, 1379
DpnI GATC 4 cut(s) 456, 585, 1013, 1374
DpnII GATC 4 cut(s) 454, 583, 1011, 1372
EaeI YGGCCR 1 cut(s) 347
Eco105I TACGTA 1 cut(s) 1200
Eco130I CCWWGG 4 cut(s) 154, 171, 685, 1441
Eco47I GGWCC 2 cut(s) 322, 925
Eco57I CTGAAG 2 cut(s) 479, 1391
EcoT14I CCWWGG 4 cut(s) 154, 171, 685, 1441
EcoT22I ATGCAT 2 cut(s) 724, 1480
ErhI CCWWGG 4 cut(s) 154, 171, 685, 1441
Fnu4HI GCNGC 6 cut(s) 395, 637, 840, 848, 1596, 1604
FokI GGATG 3 cut(s) 692, 1106, 1448
Fsp4HI GCNGC 6 cut(s) 395, 637, 840, 848, 1596, 1604
FspBI CTAG 3 cut(s) 47, 336, 1627
GlaI GCGC 1 cut(s) 1531
GluI GCNGC 6 cut(s) 395, 637, 840, 848, 1596, 1604
HaeII RGCGCY 1 cut(s) 1533
HaeIII GGCC 3 cut(s) 302, 349, 704
HapII CCGG 4 cut(s) 346, 915, 1038, 1426
HhaI GCGC 1 cut(s) 1532
Hin6I GCGC 1 cut(s) 1530
HinP1I GCGC 1 cut(s) 1530
HincII GTYRAC 1 cut(s) 678
HindII GTYRAC 1 cut(s) 678
HindIII AAGCTT 2 cut(s) 101, 890
HinfI GANTC 4 cut(s) 519, 608, 948, 1397
HpaII CCGG 4 cut(s) 346, 915, 1038, 1426
HphI GGTGA 4 cut(s) 202, 896, 991, 1444
Hpy166II GTNNAC 6 cut(s) 193, 678, 982, 1139, 1545, 1582
Hpy188I TCNGA 6 cut(s) 294, 459, 579, 953, 1083, 1410
Hpy188III TCNNGA 6 cut(s) 416, 444, 508, 1205, 1297, 1332
Hpy8I GTNNAC 6 cut(s) 193, 678, 982, 1139, 1545, 1582
Hpy99I CGWCG 2 cut(s) 1457, 1580
HpyCH4III ACNGT 8 cut(s) 374, 430, 560, 682, 817, 884, 1163, 1219
HpyCH4IV ACGT 3 cut(s) 448, 1135, 1199
HpyF10VI GCNNNNNNNGC 3 cut(s) 701, 809, 1565
HpyF3I CTNAG 5 cut(s) 408, 537, 590, 804, 1379
HpySE526I ACGT 3 cut(s) 448, 1135, 1199
HspAI GCGC 1 cut(s) 1530
KspI CCGCGG 1 cut(s) 924
Kzo9I GATC 4 cut(s) 454, 583, 1011, 1372
LmnI GCTCC 3 cut(s) 380, 773, 1188
Lsp1109I GCAGC 5 cut(s) 381, 851, 859, 1607, 1615
LweI GCATC 6 cut(s) 709, 1165, 1266, 1465, 1561, 1577
MaeI CTAG 3 cut(s) 47, 336, 1627
MaeII ACGT 3 cut(s) 448, 1135, 1199
MaeIII GTNAC 6 cut(s) 562, 785, 903, 1073, 1351, 1432
MalI GATC 4 cut(s) 456, 585, 1013, 1374
MboI GATC 4 cut(s) 454, 583, 1011, 1372
MboII GAAGA 6 cut(s) 188, 472, 728, 1261, 1274, 1484
MfeI CAATTG 1 cut(s) 1149
MhlI GDGCHC 2 cut(s) 372, 1185
MluCI AATT 7 cut(s) 222, 360, 479, 755, 1149, 1342, 1511
MmeI TCCRAC 2 cut(s) 349, 1135
Mph1103I ATGCAT 2 cut(s) 724, 1480
MroXI GAANNNNTTC 2 cut(s) 226, 491
MseI TTAA 3 cut(s) 32, 531, 1320
MslI CAYNNNNRTG 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 923
MspI CCGG 4 cut(s) 346, 915, 1038, 1426
MspR9I CCNGG 1 cut(s) 1427
MunI CAATTG 1 cut(s) 1149
Mva1269I GAATGC 5 cut(s) 638, 722, 1102, 1271, 1427
MvnI CGCG 1 cut(s) 923
MwoI GCNNNNNNNGC 3 cut(s) 701, 809, 1565
NciI CCSGG 1 cut(s) 1427
NcoI CCATGG 3 cut(s) 154, 685, 1441
NdeII GATC 4 cut(s) 454, 583, 1011, 1372
NlaIV GGNNCC 3 cut(s) 271, 919, 1060
NmeAIII GCCGAG 2 cut(s) 328, 1585
NmuCI GTSAC 3 cut(s) 562, 1351, 1432
NsiI ATGCAT 2 cut(s) 724, 1480
NspI RCATGY 2 cut(s) 317, 764
PaeI GCATGC 1 cut(s) 317
PagI TCATGA 2 cut(s) 415, 1204
PciI ACATGT 1 cut(s) 760
PctI GAATGC 5 cut(s) 638, 722, 1102, 1271, 1427
PdmI GAANNNNTTC 2 cut(s) 226, 491
PfeI GAWTC 4 cut(s) 519, 608, 948, 1397
Pfl23II CGTACG 2 cut(s) 425, 1214
PflFI GACNNNGTC 1 cut(s) 1457
PinAI ACCGGT 1 cut(s) 914
PkrI GCNGC 6 cut(s) 396, 638, 841, 849, 1597, 1605
Ppu21I YACGTR 1 cut(s) 1200
PscI ACATGT 1 cut(s) 760
PspLI CGTACG 2 cut(s) 425, 1214
PspN4I GGNNCC 3 cut(s) 271, 919, 1060
PspPI GGNCC 2 cut(s) 322, 925
PstI CTGCAG 2 cut(s) 849, 1605
PsyI GACNNNGTC 1 cut(s) 1457
RsaI GTAC 9 cut(s) 11, 427, 595, 666, 870, 1118, 1216, 1384, 1544
RsaNI GTAC 9 cut(s) 10, 426, 594, 665, 869, 1117, 1215, 1383, 1543
RseI CAYNNNNRTG 1 cut(s) 17
SacII CCGCGG 1 cut(s) 924
SaqAI TTAA 3 cut(s) 32, 531, 1320
SatI GCNGC 6 cut(s) 395, 637, 840, 848, 1596, 1604
Sau3AI GATC 4 cut(s) 454, 583, 1011, 1372
Sau96I GGNCC 2 cut(s) 322, 925
ScaI AGTACT 3 cut(s) 595, 1118, 1384
ScrFI CCNGG 1 cut(s) 1427
SduI GDGCHC 2 cut(s) 372, 1185
SfaNI GCATC 6 cut(s) 709, 1165, 1266, 1465, 1561, 1577
SfcI CTRYAG 2 cut(s) 845, 1601
Sfr303I CCGCGG 1 cut(s) 924
SgrBI CCGCGG 1 cut(s) 924
SinI GGWCC 2 cut(s) 322, 925
SmiMI CAYNNNNRTG 1 cut(s) 17
SnaBI TACGTA 1 cut(s) 1200
SpeI ACTAGT 1 cut(s) 46
SphI GCATGC 1 cut(s) 317
Sse9I AATT 7 cut(s) 222, 360, 479, 755, 1149, 1342, 1511
SsiI CCGC 5 cut(s) 62, 134, 637, 921, 923
SspMI CTAG 3 cut(s) 47, 336, 1627
StyD4I CCNGG 1 cut(s) 1425
StyI CCWWGG 4 cut(s) 154, 171, 685, 1441
TaaI ACNGT 8 cut(s) 374, 430, 560, 682, 817, 884, 1163, 1219
TaiI ACGT 3 cut(s) 451, 1138, 1202
TaqI TCGA 7 cut(s) 163, 240, 714, 726, 768, 1113, 1260
TaqII GACCGA 3 cut(s) 581, 659, 1370
TasI AATT 7 cut(s) 222, 360, 479, 755, 1149, 1342, 1511
TatI WGTACW 4 cut(s) 593, 664, 1116, 1382
TauI GCSGC 1 cut(s) 639
TfiI GAWTC 4 cut(s) 519, 608, 948, 1397
Tru1I TTAA 3 cut(s) 32, 531, 1320
Tru9I TTAA 3 cut(s) 32, 531, 1320
TscAI CASTG 2 cut(s) 1002, 1150
TseFI GTSAC 3 cut(s) 562, 1351, 1432
TseI GCWGC 5 cut(s) 394, 839, 847, 1595, 1603
Tsp45I GTSAC 3 cut(s) 562, 1351, 1432
TspGWI ACGGA 2 cut(s) 224, 1013
TspRI CASTG 2 cut(s) 1002, 1150
Tth111I GACNNNGTC 1 cut(s) 1457
VpaK11BI GGWCC 2 cut(s) 322, 925
XceI RCATGY 2 cut(s) 317, 764
XmnI GAANNNNTTC 2 cut(s) 226, 491
XspI CTAG 3 cut(s) 47, 336, 1627
ZrmI AGTACT 3 cut(s) 595, 1118, 1384
Zsp2I ATGCAT 2 cut(s) 724, 1480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.