pycom02g06430

Glycerol-3-phosphate dehydrogenase NAD( )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
N/A
Physical Location & Seq
Reverse (-)
4485481 .. 4486737
1257 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 825 bp
ATGCAGAGTCTCTTTTCAGAAAGCTCATCGCCTACTAACAATGATCCAAACATCCAGATTTGTCACATTACCTCTTACGCTGTGATCTTGGATAACGACACTATTGAACAAAGAGGACCACGCCCGCTTCTTCGACGGGCTCTACAACGACACTGTTCCCGGCCATTTCTCTTATGTATGCAACTCACCGAGGATGTGAATGTTGGAGTTCTTACCTTTTTGGGACTCCATGGACCAAGATTTGCGGTGCCAGAAAATATAATTGCAACAACTGATGCAGAAGCTGCTTTGCTGGGTGCCGATTATTGCTTTCATGCTGTCCCTGTGCAGGTTTTTTTCAGCTTGCCTTTCATATCAGTTAGCAAGAGTTTAGAGGTCAATACGCTGAGGACCGTGTCTCAGATCACTCCTCAAGCGTTGAAGAATCATCGGGAACCTTTCATTGCACTATCAGGGCCTTCTTTTGCTCTGGAGCTGATGAATAAATTACCAACAGGTATGTGGAAAAAAGAAACAACATTGTTGAATCAACATTCCACTAAATCATGTTTTAAAGAATTGGCAAATGCAACTCAGCAGCTTCTGGCTTGTGACTATCTGAGAATCAGCACTTCAAGGTTGTGGCAGATCCCAGGCCCATCTTCACGTGAAGATGGGCGCGAAGCCGGCAACAACCACCGGTCTACAAAGTTCCATGAATCACGTGGTAACGTTATGCGGTTTACTAGGTGGTTGAAGTATAATGTGAAGATGCCGGTTTTGAGGGCAGTAGCTCGGATTATTGACAACCAACGGACCCCGAAGAAAGCTGTTCTTCAAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01110 ko04011 map00564 map01110 map04011
Pfam Domains
Protein Families
NAD_Gly3P_dh_C NAD_Gly3P_dh_N

Protein Analysis

275

Amino Acids

30.84

Weight (kDa)

9.56

Isoelectric Point (pI)

58.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_Gly3P_dh_N PF01210 79 - 167 2.5e-09 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 319
AccB1I GGYRCC 2 cut(s) 247, 296
AccI GTMKAC 1 cut(s) 683
AccII CGCG 1 cut(s) 660
AciI CCGC 3 cut(s) 125, 245, 718
AclI AACGTT 1 cut(s) 711
AclWI GGATC 2 cut(s) 38, 622
AcoI YGGCCR 1 cut(s) 161
AcvI CACGTG 2 cut(s) 647, 704
AfiI CCNNNNNNNGG 1 cut(s) 328
AgeI ACCGGT 1 cut(s) 678
AgsI TTSAA 6 cut(s) 107, 421, 526, 615, 736, 818
AjnI CCWGG 1 cut(s) 631
AluBI AGCT 7 cut(s) 24, 284, 342, 475, 580, 773, 809
AluI AGCT 7 cut(s) 24, 284, 342, 475, 580, 773, 809
Alw26I GTCTC 2 cut(s) 14, 402
AlwI GGATC 2 cut(s) 38, 622
AlwNI CAGNNNCTG 2 cut(s) 284, 583
AoxI GGCC 3 cut(s) 161, 455, 634
ApeKI GCWGC 2 cut(s) 284, 577
AsiGI ACCGGT 1 cut(s) 678
AspLEI GCGC 1 cut(s) 660
AspS9I GGNCC 6 cut(s) 116, 233, 390, 455, 635, 795
AsuC2I CCSGG 1 cut(s) 160
AsuHPI GGTGA 1 cut(s) 178
AvaII GGWCC 4 cut(s) 116, 233, 390, 795
BanI GGYRCC 2 cut(s) 247, 296
BanII GRGCYC 1 cut(s) 142
BbrPI CACGTG 2 cut(s) 647, 704
BbvCI CCTCAGC 1 cut(s) 386
BbvI GCAGC 2 cut(s) 271, 589
BccI CCATC 2 cut(s) 646, 647
BciT130I CCWGG 1 cut(s) 633
BcnI CCSGG 1 cut(s) 160
BcoDI GTCTC 2 cut(s) 14, 402
BfaI CTAG 1 cut(s) 726
BfuAI ACCTGC 1 cut(s) 319
BisI GCNGC 2 cut(s) 285, 578
BlsI GCNGC 2 cut(s) 286, 579
Bme1390I CCNGG 2 cut(s) 160, 633
Bme18I GGWCC 4 cut(s) 116, 233, 390, 795
BmgT120I GGNCC 6 cut(s) 116, 233, 390, 455, 635, 795
BmiI GGNNCC 4 cut(s) 249, 298, 435, 797
BmrFI CCNGG 2 cut(s) 160, 633
BmsI GCATC 2 cut(s) 265, 741
BpmI CTGGAG 1 cut(s) 491
Bpu10I CCTNAGC 1 cut(s) 386
BpuEI CTTGAG 1 cut(s) 396
BpuMI CCSGG 1 cut(s) 160
BsaAI YACGTR 2 cut(s) 647, 704
BsaJI CCNNGG 3 cut(s) 189, 229, 631
BsaWI WCCGGW 1 cut(s) 678
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse118I RCCGGY 3 cut(s) 665, 678, 754
Bse3DI GCAATG 1 cut(s) 441
BseBI CCWGG 1 cut(s) 633
BseDI CCNNGG 3 cut(s) 189, 229, 631
BseGI GGATG 2 cut(s) 51, 199
BseLI CCNNNNNNNGG 1 cut(s) 328
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 4 cut(s) 377, 413, 587, 590
BseRI GAGGAG 1 cut(s) 399
BseXI GCAGC 2 cut(s) 271, 589
BseYI CCCAGC 1 cut(s) 292
BsgI GTGCAG 1 cut(s) 347
Bsh1236I CGCG 1 cut(s) 660
BshFI GGCC 3 cut(s) 163, 457, 636
BshNI GGYRCC 2 cut(s) 247, 296
BshTI ACCGGT 1 cut(s) 678
BsiSI CCGG 4 cut(s) 160, 666, 679, 755
BslFI GGGAC 2 cut(s) 237, 305
BslI CCNNNNNNNGG 1 cut(s) 328
BsmAI GTCTC 2 cut(s) 14, 402
BsmFI GGGAC 2 cut(s) 237, 305
BsnI GGCC 3 cut(s) 163, 457, 636
Bsp1286I GDGCHC 1 cut(s) 142
Bsp143I GATC 4 cut(s) 43, 84, 402, 627
Bsp19I CCATGG 1 cut(s) 229
BspACI CCGC 3 cut(s) 125, 245, 718
BspANI GGCC 3 cut(s) 163, 457, 636
BspCNI CTCAG 4 cut(s) 378, 412, 586, 591
BspFNI CGCG 1 cut(s) 660
BspLI GGNNCC 4 cut(s) 249, 298, 435, 797
BspMI ACCTGC 1 cut(s) 319
BspPI GGATC 2 cut(s) 38, 622
BspT107I GGYRCC 2 cut(s) 247, 296
BsrDI GCAATG 1 cut(s) 441
BsrFI RCCGGY 3 cut(s) 665, 678, 754
BssAI RCCGGY 3 cut(s) 665, 678, 754
BssECI CCNNGG 3 cut(s) 189, 229, 631
BssMI GATC 4 cut(s) 43, 84, 402, 627
BssT1I CCWWGG 1 cut(s) 229
Bst2UI CCWGG 1 cut(s) 633
Bst4CI ACNGT 2 cut(s) 155, 394
BstAPI GCANNNNNTGC 1 cut(s) 284
BstBAI YACGTR 2 cut(s) 647, 704
BstC8I GCNNGC 3 cut(s) 125, 344, 667
BstDEI CTNAG 4 cut(s) 386, 399, 573, 599
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 2 cut(s) 51, 199
BstFNI CGCG 1 cut(s) 660
BstHHI GCGC 1 cut(s) 660
BstKTI GATC 4 cut(s) 46, 87, 405, 630
BstMAI GTCTC 2 cut(s) 14, 402
BstMBI GATC 4 cut(s) 43, 84, 402, 627
BstMWI GCNNNNNNNGC 2 cut(s) 284, 666
BstNI CCWGG 1 cut(s) 633
BstSCI CCNGG 2 cut(s) 158, 631
BstUI CGCG 1 cut(s) 660
BstV1I GCAGC 2 cut(s) 271, 589
BstX2I RGATCY 1 cut(s) 627
BstYI RGATCY 1 cut(s) 627
BsuRI GGCC 3 cut(s) 163, 457, 636
BtgI CCRYGG 1 cut(s) 229
BtgZI GCGATG 1 cut(s) 12
BtsCI GGATG 2 cut(s) 51, 199
BtsIMutI CAGTG 1 cut(s) 151
BveI ACCTGC 1 cut(s) 319
Cac8I GCNNGC 3 cut(s) 125, 344, 667
CaiI CAGNNNCTG 2 cut(s) 284, 583
CfoI GCGC 1 cut(s) 660
Cfr10I RCCGGY 3 cut(s) 665, 678, 754
Cfr13I GGNCC 6 cut(s) 116, 233, 390, 455, 635, 795
CspAI ACCGGT 1 cut(s) 678
CviAII CATG 4 cut(s) 230, 314, 546, 695
DdeI CTNAG 4 cut(s) 386, 399, 573, 599
DpnI GATC 4 cut(s) 45, 86, 404, 629
DpnII GATC 4 cut(s) 43, 84, 402, 627
DraI TTTAAA 1 cut(s) 553
EaeI YGGCCR 1 cut(s) 161
Eco130I CCWWGG 1 cut(s) 229
Eco24I GRGCYC 1 cut(s) 142
Eco47I GGWCC 4 cut(s) 116, 233, 390, 795
Eco72I CACGTG 2 cut(s) 647, 704
EcoO109I RGGNCCY 1 cut(s) 455
EcoRII CCWGG 1 cut(s) 631
EcoT14I CCWWGG 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 229
FaeI CATG 4 cut(s) 233, 317, 549, 698
FalI AAGNNNNNCTT 1 cut(s) 798
FaqI GGGAC 2 cut(s) 237, 305
FatI CATG 4 cut(s) 229, 313, 545, 694
FauI CCCGC 1 cut(s) 132
FblI GTMKAC 1 cut(s) 683
Fnu4HI GCNGC 2 cut(s) 285, 578
FokI GGATG 2 cut(s) 38, 206
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 2 cut(s) 285, 578
FspBI CTAG 1 cut(s) 726
GlaI GCGC 1 cut(s) 659
GluI GCNGC 2 cut(s) 285, 578
GsaI CCCAGC 1 cut(s) 296
GsuI CTGGAG 1 cut(s) 491
HaeIII GGCC 3 cut(s) 163, 457, 636
HapII CCGG 4 cut(s) 160, 666, 679, 755
HhaI GCGC 1 cut(s) 660
Hin1II CATG 4 cut(s) 233, 317, 549, 698
Hin6I GCGC 1 cut(s) 658
HinP1I GCGC 1 cut(s) 658
HinfI GANTC 6 cut(s) 7, 225, 424, 526, 603, 698
HpaII CCGG 4 cut(s) 160, 666, 679, 755
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 2 cut(s) 684, 723
Hpy188I TCNGA 4 cut(s) 19, 402, 600, 777
Hpy188III TCNNGA 3 cut(s) 55, 431, 470
Hpy8I GTNNAC 2 cut(s) 684, 723
Hpy99I CGWCG 1 cut(s) 138
HpyAV CCTTC 1 cut(s) 468
HpyCH4III ACNGT 2 cut(s) 155, 394
HpyCH4IV ACGT 3 cut(s) 646, 703, 711
HpyCH4V TGCA 7 cut(s) 4, 181, 266, 278, 328, 446, 569
HpyF10VI GCNNNNNNNGC 2 cut(s) 284, 666
HpyF3I CTNAG 4 cut(s) 386, 399, 573, 599
HpySE526I ACGT 3 cut(s) 646, 703, 711
Hsp92II CATG 4 cut(s) 233, 317, 549, 698
HspAI GCGC 1 cut(s) 658
KroI GCCGGC 1 cut(s) 665
KroNI GCCGGC 1 cut(s) 667
Kzo9I GATC 4 cut(s) 43, 84, 402, 627
LmnI GCTCC 1 cut(s) 472
Lsp1109I GCAGC 2 cut(s) 271, 589
LweI GCATC 2 cut(s) 265, 741
MaeI CTAG 1 cut(s) 726
MaeII ACGT 3 cut(s) 646, 703, 711
MaeIII GTNAC 3 cut(s) 62, 590, 707
MalI GATC 4 cut(s) 45, 86, 404, 629
MboI GATC 4 cut(s) 43, 84, 402, 627
MboII GAAGA 7 cut(s) 122, 433, 633, 662, 760, 806, 814
MflI RGATCY 1 cut(s) 627
MhlI GDGCHC 1 cut(s) 142
MluCI AATT 3 cut(s) 261, 485, 557
MlyI GAGTC 2 cut(s) 16, 219
MmeI TCCRAC 1 cut(s) 184
MnlI CCTC 7 cut(s) 82, 107, 184, 367, 381, 420, 756
MroNI GCCGGC 1 cut(s) 665
MseI TTAA 1 cut(s) 552
MspI CCGG 4 cut(s) 160, 666, 679, 755
MspR9I CCNGG 2 cut(s) 160, 633
MvaI CCWGG 1 cut(s) 633
MvnI CGCG 1 cut(s) 660
MwoI GCNNNNNNNGC 2 cut(s) 284, 666
NaeI GCCGGC 1 cut(s) 667
NciI CCSGG 1 cut(s) 160
NcoI CCATGG 1 cut(s) 229
NdeII GATC 4 cut(s) 43, 84, 402, 627
NgoMIV GCCGGC 1 cut(s) 665
NlaIII CATG 4 cut(s) 233, 317, 549, 698
NlaIV GGNNCC 4 cut(s) 249, 298, 435, 797
NmuCI GTSAC 2 cut(s) 62, 590
PdiI GCCGGC 1 cut(s) 667
PfeI GAWTC 4 cut(s) 424, 526, 603, 698
PflFI GACNNNGTC 1 cut(s) 394
PinAI ACCGGT 1 cut(s) 678
PkrI GCNGC 2 cut(s) 286, 579
PleI GAGTC 2 cut(s) 15, 219
PmaCI CACGTG 2 cut(s) 647, 704
PmlI CACGTG 2 cut(s) 647, 704
PpsI GAGTC 2 cut(s) 15, 219
Ppu21I YACGTR 2 cut(s) 647, 704
Psp1406I AACGTT 1 cut(s) 711
Psp6I CCWGG 1 cut(s) 631
PspCI CACGTG 2 cut(s) 647, 704
PspFI CCCAGC 1 cut(s) 292
PspGI CCWGG 1 cut(s) 631
PspN4I GGNNCC 4 cut(s) 249, 298, 435, 797
PspPI GGNCC 6 cut(s) 116, 233, 390, 455, 635, 795
PstNI CAGNNNCTG 2 cut(s) 284, 583
PsuI RGATCY 1 cut(s) 627
PsyI GACNNNGTC 1 cut(s) 394
SaqAI TTAA 1 cut(s) 552
SatI GCNGC 2 cut(s) 285, 578
Sau3AI GATC 4 cut(s) 43, 84, 402, 627
Sau96I GGNCC 6 cut(s) 116, 233, 390, 455, 635, 795
SchI GAGTC 2 cut(s) 16, 219
ScrFI CCNGG 2 cut(s) 160, 633
SduI GDGCHC 1 cut(s) 142
SfaNI GCATC 2 cut(s) 265, 741
SinI GGWCC 4 cut(s) 116, 233, 390, 795
SmlI CTYRAG 1 cut(s) 411
SmoI CTYRAG 1 cut(s) 411
Sse9I AATT 3 cut(s) 261, 485, 557
SsiI CCGC 3 cut(s) 125, 245, 718
SspMI CTAG 1 cut(s) 726
StyD4I CCNGG 2 cut(s) 158, 631
StyI CCWWGG 1 cut(s) 229
TaaI ACNGT 2 cut(s) 155, 394
TaiI ACGT 3 cut(s) 649, 706, 714
TaqI TCGA 1 cut(s) 133
TasI AATT 3 cut(s) 261, 485, 557
TfiI GAWTC 4 cut(s) 424, 526, 603, 698
Tru1I TTAA 1 cut(s) 552
Tru9I TTAA 1 cut(s) 552
TscAI CASTG 1 cut(s) 158
TseFI GTSAC 2 cut(s) 62, 590
TseI GCWGC 2 cut(s) 284, 577
Tsp45I GTSAC 2 cut(s) 62, 590
TspDTI ATGAA 5 cut(s) 302, 340, 430, 494, 711
TspGWI ACGGA 1 cut(s) 808
TspRI CASTG 1 cut(s) 158
Tth111I GACNNNGTC 1 cut(s) 394
VpaK11BI GGWCC 4 cut(s) 116, 233, 390, 795
XcmI CCANNNNNNNNNTGG 2 cut(s) 498, 701
XmiI GTMKAC 1 cut(s) 683
XspI CTAG 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.