pycom02g19180
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
N/A
Physical Location & Seq
Forward (+)
17138972 .. 17140894
1923 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1608 bp
ATGCAAACAGATCATGTGAACTTTGGACAGCTTGCACACAGCAGCTCACATCTCTGCTGTCTTTCAACATACTTTCAGCTAGCACATGGAGGAAACTTTAGTCTTTGTAGTTGCAGCATGTCCTGTTTTTATTTGAATTTCCAACACTTATCTTCTTCCCTTGTAAGGTTCTATGCATCTGTGAATAAAATATCAGGAAGTACCCCAAATGGGATCGAAAATCTTCAGAACCTGCAGAGTCTATGGCTGTCTACAAACCAACTTTCGGGTCACATCCCCAGCGATCTAGGAAAGCTTCAAAAGTTGTATACCTTAGATTTGGGTAACAACTCTCTCCGGGAACATCTACAAGAAAACAAGCTTCAAGGCAATATCCCTTCAAGTTTAGCTCAGTGTCAAAATCTGAAGATCTTGTCTCTTGGTATCAACAATTTCAGTGGTACCATTCCCCCAGAAGTGTTTGGTCTCTCGTCTTCATATGTTGTTTTGGATTTGTCAGGAAATCTTCTGACTGGTTCCATTCCAAAGGAAATCGGAAGTTTAATAAATATGGAGCACCTTGATGTTTCTGAAAACAAGGTATCTGGGGGAATTCCGGCAAGTCTCAGTAGTTGTATAAAAATAGAATTCTTAAACATGCAAAGGAATCTCCTCCAAGGTACCATTCCATTGTCTCTGGCCTCACTAAGAGGTATTCGAGAATTATATCTCGACCACAACAACTTATCAGGTACGATTCCAAAATTCTTGGAGACTTTTGAGTTTGTGCAATCTTTGAATCTTTCTTACAATAATTTCGAGGGCGAGGTACCAATGGAAGGAGTTTTCAAGAATGCAACTGCGACATTGGTTGAAGGAAATAGTAAGCTTTGTGGGGGCATACCTGAGTTTCAACTGCCAGAATGCAAACTTCAACATCAAGCAAAGGGAGGATTGAGCCGAACCTCCAATTTGATAATCTCTAACCTCCAAGTTGATAATCTCTCTAGTCAGTGGGCTACTTGGAATAAGTTTTGCACTATTAAGCAAACTTCAAGTACTGCTGAATGTGAGGCTTTGAGAAATATTAGACACCGTAATCTTTTAAAGGTTCTATCGGCATGTTCAAGTGTTGATTACCGTGGCTTTGATTTCAAGGCCATAATCTACAAATTTATGGTTAATGGGAGCTTAGAGGAATGGTTGCACCCAGCTCAGTTAATTGGTGAGACAAATGAGAGACCCAACCTTTTCGCAGAGCTTCATAACCATTGTGAATCACCAATAGTTCACTGTGACCTAAAACCGAGCAATGTTCTTCTCAATGATGACATGATTGGAAATGTAGGTGACTTTGGTTTGGTAAGATTCCTTCCTGGAACTTCAGAAAATGGTTCTGGTAAACAATCTATTTCCACCGGTCTTAAGGGAACTATTGTTCTCATTATCTTGTCTTTAAATAGCTTTTGGCATATAAATTTTGGAGGTAAGGCCCAGTGGGGGCACATGCCCCCTCTCATCCCTTTTTTTTTTTTTTTTTCATTACGAATTATTCAATTAATCATTCCCATTTTTTTGCCCCTCCATCTTGTATATTTTTCTCACGCACTAATGTCTAAACCTTGTTGA

Protein Analysis

536

Amino Acids

59.17

Weight (kDa)

6.67

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 342 - 456 2.9e-10 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 343 - 455 2.9e-08 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 240
Acc65I GGTACC 3 cut(s) 440, 659, 808
AccB1I GGYRCC 3 cut(s) 440, 659, 808
AccI GTMKAC 2 cut(s) 251, 308
AclWI GGATC 1 cut(s) 221
AcsI RAATTY 6 cut(s) 136, 591, 626, 743, 1151, 1456
AcuI CTGAAG 3 cut(s) 209, 425, 1347
AfaI GTAC 6 cut(s) 202, 442, 661, 733, 810, 1039
AfiI CCNNNNNNNGG 5 cut(s) 165, 210, 265, 818, 1479
AflII CTTAAG 1 cut(s) 1403
AgeI ACCGGT 1 cut(s) 1397
AjnI CCWGG 1 cut(s) 1354
AjuI GAANNNNNNNTTGG 2 cut(s) 648, 680
AloI GAACNNNNNNTCC 2 cut(s) 1401, 1433
Alw21I GWGCWC 1 cut(s) 558
Alw26I GTCTC 7 cut(s) 420, 470, 608, 678, 746, 1202, 1213
AlwI GGATC 1 cut(s) 221
AlwNI CAGNNNCTG 1 cut(s) 232
AoxI GGCC 3 cut(s) 678, 1137, 1470
ApeKI GCWGC 2 cut(s) 42, 114
ApoI RAATTY 6 cut(s) 136, 591, 626, 743, 1151, 1456
AseI ATTAAT 1 cut(s) 1538
AsiGI ACCGGT 1 cut(s) 1397
Asp700I GAANNNNTTC 1 cut(s) 222
Asp718I GGTACC 3 cut(s) 440, 659, 808
AspS9I GGNCC 1 cut(s) 1471
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 3 cut(s) 1217, 1251, 1340
AsuNHI GCTAGC 1 cut(s) 79
BaeGI GKGCMC 1 cut(s) 1485
BanI GGYRCC 3 cut(s) 440, 659, 808
BarI GAAGNNNNNNTAC 2 cut(s) 1335, 1367
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 1 cut(s) 558
BbvI GCAGC 2 cut(s) 54, 126
BccI CCATC 1 cut(s) 1572
BciT130I CCWGG 1 cut(s) 1356
BcnI CCSGG 1 cut(s) 338
BcoDI GTCTC 7 cut(s) 420, 470, 608, 678, 746, 1202, 1213
BfaI CTAG 3 cut(s) 80, 287, 987
BfmI CTRYAG 1 cut(s) 233
BfrI CTTAAG 1 cut(s) 1403
BfuAI ACCTGC 1 cut(s) 240
BglII AGATCT 1 cut(s) 408
BisI GCNGC 2 cut(s) 43, 115
BlsI GCNGC 2 cut(s) 44, 116
BmcAI AGTACT 1 cut(s) 1039
Bme1390I CCNGG 2 cut(s) 338, 1356
BmgT120I GGNCC 1 cut(s) 1471
BmiI GGNNCC 4 cut(s) 442, 517, 661, 810
BmrFI CCNGG 2 cut(s) 338, 1356
BmrI ACTGGG 1 cut(s) 1468
BmsI GCATC 1 cut(s) 185
BmtI GCTAGC 1 cut(s) 83
BmuI ACTGGG 1 cut(s) 1468
BpiI GAAGAC 1 cut(s) 465
BpuMI CCSGG 1 cut(s) 338
BsaI GGTCTC 2 cut(s) 470, 1213
BsaJI CCNNGG 2 cut(s) 655, 1120
BsaWI WCCGGW 1 cut(s) 1397
Bsc4I CCNNNNNNNGG 5 cut(s) 165, 210, 265, 818, 1479
Bse118I RCCGGY 1 cut(s) 1397
Bse1I ACTGG 2 cut(s) 517, 1474
Bse3DI GCAATG 1 cut(s) 1297
BseBI CCWGG 1 cut(s) 1356
BseDI CCNNGG 2 cut(s) 655, 1120
BseGI GGATG 2 cut(s) 273, 1497
BseLI CCNNNNNNNGG 5 cut(s) 165, 210, 265, 818, 1479
BseMI GCAATG 1 cut(s) 1297
BseMII CTCAG 4 cut(s) 404, 619, 876, 1208
BseNI ACTGG 2 cut(s) 517, 1474
BseRI GAGGAG 1 cut(s) 641
BseSI GKGCMC 1 cut(s) 1485
BseXI GCAGC 2 cut(s) 54, 126
BseYI CCCAGC 2 cut(s) 278, 1189
BshFI GGCC 3 cut(s) 680, 1139, 1472
BshNI GGYRCC 3 cut(s) 440, 659, 808
BshTI ACCGGT 1 cut(s) 1397
BsiHKAI GWGCWC 1 cut(s) 558
BsiSI CCGG 3 cut(s) 337, 596, 1398
BslI CCNNNNNNNGG 5 cut(s) 165, 210, 265, 818, 1479
BsmAI GTCTC 7 cut(s) 420, 470, 608, 678, 746, 1202, 1213
BsmI GAATGC 2 cut(s) 838, 908
BsnI GGCC 3 cut(s) 680, 1139, 1472
Bso31I GGTCTC 2 cut(s) 470, 1213
Bsp1286I GDGCHC 2 cut(s) 558, 1485
Bsp143I GATC 4 cut(s) 10, 213, 283, 408
BspANI GGCC 3 cut(s) 680, 1139, 1472
BspCNI CTCAG 4 cut(s) 403, 618, 877, 1207
BspLI GGNNCC 4 cut(s) 442, 517, 661, 810
BspMAI CTGCAG 1 cut(s) 237
BspMI ACCTGC 1 cut(s) 240
BspOI GCTAGC 1 cut(s) 83
BspPI GGATC 1 cut(s) 221
BspT107I GGYRCC 3 cut(s) 440, 659, 808
BspTI CTTAAG 1 cut(s) 1403
BspTNI GGTCTC 2 cut(s) 470, 1213
BsrDI GCAATG 1 cut(s) 1297
BsrFI RCCGGY 1 cut(s) 1397
BsrI ACTGG 2 cut(s) 517, 1474
BssAI RCCGGY 1 cut(s) 1397
BssECI CCNNGG 2 cut(s) 655, 1120
BssMI GATC 4 cut(s) 10, 213, 283, 408
BssNAI GTATAC 1 cut(s) 309
BssT1I CCWWGG 1 cut(s) 655
Bst1107I GTATAC 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 1356
Bst4CI ACNGT 3 cut(s) 1076, 1121, 1274
BstAFI CTTAAG 1 cut(s) 1403
BstC8I GCNNGC 2 cut(s) 33, 81
BstDEI CTNAG 7 cut(s) 313, 390, 605, 686, 885, 1171, 1194
BstDSI CCRYGG 1 cut(s) 1120
BstF5I GGATG 2 cut(s) 273, 1497
BstKTI GATC 4 cut(s) 13, 216, 286, 411
BstMAI GTCTC 7 cut(s) 420, 470, 608, 678, 746, 1202, 1213
BstMBI GATC 4 cut(s) 10, 213, 283, 408
BstNI CCWGG 1 cut(s) 1356
BstNSI RCATGY 4 cut(s) 121, 640, 1104, 1489
BstSCI CCNGG 2 cut(s) 336, 1354
BstSFI CTRYAG 1 cut(s) 233
BstSLI GKGCMC 1 cut(s) 1485
BstV1I GCAGC 2 cut(s) 54, 126
BstV2I GAAGAC 1 cut(s) 465
BstX2I RGATCY 1 cut(s) 408
BstYI RGATCY 1 cut(s) 408
BstZ17I GTATAC 1 cut(s) 309
BsuRI GGCC 3 cut(s) 680, 1139, 1472
BtgI CCRYGG 1 cut(s) 1120
BtsCI GGATG 2 cut(s) 273, 1497
BtsIMutI CAGTG 5 cut(s) 398, 442, 998, 1270, 1481
BveI ACCTGC 1 cut(s) 240
Cac8I GCNNGC 2 cut(s) 33, 81
CaiI CAGNNNCTG 1 cut(s) 232
Cfr10I RCCGGY 1 cut(s) 1397
Cfr13I GGNCC 1 cut(s) 1471
Csp6I GTAC 6 cut(s) 201, 441, 660, 732, 809, 1038
CspAI ACCGGT 1 cut(s) 1397
CspCI CAANNNNNGTGG 2 cut(s) 418, 453
CviAII CATG 7 cut(s) 14, 86, 118, 637, 1101, 1312, 1486
CviQI GTAC 6 cut(s) 201, 441, 660, 732, 809, 1038
DdeI CTNAG 7 cut(s) 313, 390, 605, 686, 885, 1171, 1194
DpnI GATC 4 cut(s) 12, 215, 285, 410
DpnII GATC 4 cut(s) 10, 213, 283, 408
DraI TTTAAA 2 cut(s) 1086, 1437
Eco130I CCWWGG 1 cut(s) 655
Eco31I GGTCTC 2 cut(s) 470, 1213
Eco57I CTGAAG 3 cut(s) 209, 425, 1347
EcoRI GAATTC 2 cut(s) 591, 626
EcoRII CCWGG 1 cut(s) 1354
EcoT14I CCWWGG 1 cut(s) 655
EcoT22I ATGCAT 1 cut(s) 178
ErhI CCWWGG 1 cut(s) 655
FaeI CATG 7 cut(s) 17, 89, 121, 640, 1104, 1315, 1489
FatI CATG 7 cut(s) 13, 85, 117, 636, 1100, 1311, 1485
FauNDI CATATG 1 cut(s) 478
FblI GTMKAC 2 cut(s) 251, 308
Fnu4HI GCNGC 2 cut(s) 43, 115
FokI GGATG 2 cut(s) 260, 1484
Fsp4HI GCNGC 2 cut(s) 43, 115
FspBI CTAG 3 cut(s) 80, 287, 987
GluI GCNGC 2 cut(s) 43, 115
GsaI CCCAGC 2 cut(s) 282, 1193
HaeIII GGCC 3 cut(s) 680, 1139, 1472
HapII CCGG 3 cut(s) 337, 596, 1398
Hin1II CATG 7 cut(s) 17, 89, 121, 640, 1104, 1315, 1489
HindIII AAGCTT 3 cut(s) 293, 359, 866
HinfI GANTC 6 cut(s) 238, 646, 736, 778, 1256, 1347
HpaII CCGG 3 cut(s) 337, 596, 1398
HphI GGTGA 3 cut(s) 1217, 1251, 1340
Hpy166II GTNNAC 5 cut(s) 19, 252, 309, 1270, 1382
Hpy188I TCNGA 6 cut(s) 228, 405, 510, 536, 571, 1366
Hpy188III TCNNGA 5 cut(s) 195, 498, 698, 710, 829
Hpy8I GTNNAC 5 cut(s) 19, 252, 309, 1270, 1382
HpyAV CCTTC 4 cut(s) 387, 812, 848, 1361
HpyCH4III ACNGT 3 cut(s) 1076, 1121, 1274
HpyF3I CTNAG 7 cut(s) 313, 390, 605, 686, 885, 1171, 1194
Hsp92II CATG 7 cut(s) 17, 89, 121, 640, 1104, 1315, 1489
KpnI GGTACC 3 cut(s) 444, 663, 812
Kzo9I GATC 4 cut(s) 10, 213, 283, 408
LmnI GCTCC 2 cut(s) 553, 1167
Lsp1109I GCAGC 2 cut(s) 54, 126
LweI GCATC 1 cut(s) 185
MaeI CTAG 3 cut(s) 80, 287, 987
MaeIII GTNAC 4 cut(s) 269, 323, 1274, 1328
MalI GATC 4 cut(s) 12, 215, 285, 410
MboI GATC 4 cut(s) 10, 213, 283, 408
MboII GAAGA 7 cut(s) 144, 147, 215, 418, 465, 497, 1289
MflI RGATCY 1 cut(s) 408
MhlI GDGCHC 2 cut(s) 558, 1485
MlyI GAGTC 1 cut(s) 247
MmeI TCCRAC 1 cut(s) 166
Mph1103I ATGCAT 1 cut(s) 178
MroXI GAANNNNTTC 1 cut(s) 222
MseI TTAA 9 cut(s) 542, 632, 1023, 1085, 1161, 1199, 1404, 1436, 1538
MslI CAYNNNNRTG 1 cut(s) 561
MspCI CTTAAG 1 cut(s) 1403
MspI CCGG 3 cut(s) 337, 596, 1398
MspR9I CCNGG 2 cut(s) 338, 1356
Mva1269I GAATGC 2 cut(s) 838, 908
MvaI CCWGG 1 cut(s) 1356
NciI CCSGG 1 cut(s) 338
NdeI CATATG 1 cut(s) 478
NdeII GATC 4 cut(s) 10, 213, 283, 408
NheI GCTAGC 1 cut(s) 79
NlaIII CATG 7 cut(s) 17, 89, 121, 640, 1104, 1315, 1489
NlaIV GGNNCC 4 cut(s) 442, 517, 661, 810
NmuCI GTSAC 3 cut(s) 269, 1274, 1328
NsiI ATGCAT 1 cut(s) 178
NspI RCATGY 4 cut(s) 121, 640, 1104, 1489
PctI GAATGC 2 cut(s) 838, 908
PdmI GAANNNNTTC 1 cut(s) 222
PfeI GAWTC 5 cut(s) 646, 736, 778, 1256, 1347
PfoI TCCNGGA 2 cut(s) 336, 1354
PinAI ACCGGT 1 cut(s) 1397
PkrI GCNGC 2 cut(s) 44, 116
PleI GAGTC 1 cut(s) 246
PpsI GAGTC 1 cut(s) 246
PshBI ATTAAT 1 cut(s) 1538
Psp6I CCWGG 1 cut(s) 1354
PspFI CCCAGC 2 cut(s) 278, 1189
PspGI CCWGG 1 cut(s) 1354
PspN4I GGNNCC 4 cut(s) 442, 517, 661, 810
PspPI GGNCC 1 cut(s) 1471
PstI CTGCAG 1 cut(s) 237
PstNI CAGNNNCTG 1 cut(s) 232
PsuI RGATCY 1 cut(s) 408
RsaI GTAC 6 cut(s) 202, 442, 661, 733, 810, 1039
RsaNI GTAC 6 cut(s) 201, 441, 660, 732, 809, 1038
RseI CAYNNNNRTG 1 cut(s) 561
SaqAI TTAA 9 cut(s) 542, 632, 1023, 1085, 1161, 1199, 1404, 1436, 1538
SatI GCNGC 2 cut(s) 43, 115
Sau3AI GATC 4 cut(s) 10, 213, 283, 408
Sau96I GGNCC 1 cut(s) 1471
ScaI AGTACT 1 cut(s) 1039
SchI GAGTC 1 cut(s) 247
ScrFI CCNGG 2 cut(s) 338, 1356
SduI GDGCHC 2 cut(s) 558, 1485
SfaNI GCATC 1 cut(s) 185
SfcI CTRYAG 1 cut(s) 233
SmiMI CAYNNNNRTG 1 cut(s) 561
SmlI CTYRAG 1 cut(s) 1403
SmoI CTYRAG 1 cut(s) 1403
SspI AATATT 1 cut(s) 1066
SspMI CTAG 3 cut(s) 80, 287, 987
StyD4I CCNGG 2 cut(s) 336, 1354
StyI CCWWGG 1 cut(s) 655
TaaI ACNGT 3 cut(s) 1076, 1121, 1274
TaqI TCGA 4 cut(s) 216, 697, 711, 798
TatI WGTACW 1 cut(s) 1037
TfiI GAWTC 5 cut(s) 646, 736, 778, 1256, 1347
Tru1I TTAA 9 cut(s) 542, 632, 1023, 1085, 1161, 1199, 1404, 1436, 1538
Tru9I TTAA 9 cut(s) 542, 632, 1023, 1085, 1161, 1199, 1404, 1436, 1538
TscAI CASTG 5 cut(s) 398, 442, 998, 1277, 1481
TseFI GTSAC 3 cut(s) 269, 1274, 1328
TseI GCWGC 2 cut(s) 42, 114
Tsp45I GTSAC 3 cut(s) 269, 1274, 1328
TspDTI ATGAA 3 cut(s) 465, 1232, 1509
TspRI CASTG 5 cut(s) 398, 442, 998, 1277, 1481
Vha464I CTTAAG 1 cut(s) 1403
VspI ATTAAT 1 cut(s) 1538
XapI RAATTY 6 cut(s) 136, 591, 626, 743, 1151, 1456
XceI RCATGY 4 cut(s) 121, 640, 1104, 1489
XmiI GTMKAC 2 cut(s) 251, 308
XmnI GAANNNNTTC 1 cut(s) 222
XspI CTAG 3 cut(s) 80, 287, 987
ZrmI AGTACT 1 cut(s) 1039
Zsp2I ATGCAT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.